Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,048,273 A→T 5.4% T177T (ACT→ACA)  ygfB ← UPF0149 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,048,2730AT 5.4% 727.8 / 3.3 246T177T (ACT→ACA) ygfBUPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base A (71/157);  new base T (12/2);  total (87/159)
Fisher's exact test for biased strand distribution p-value = 7.05e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

CGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCC  >  CP009273/3048124‑3048422
                                                                                                                                                     |                                                                                                                                                     
  gcgcTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGt                                                                                                                                                     <  1:1339021/150‑1 (MQ=255)
   cgcTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTa                                                                                                                                                    <  1:989798/150‑1 (MQ=255)
      tGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAg                                                                                                                                                 <  2:3893222/150‑1 (MQ=255)
      tGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTGTTGGGCGGTGGGGTGCGGGTGAGTAAAg                                                                                                                                                 >  1:4135642/1‑150 (MQ=255)
        ccGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTCTGGCGCGGGGGGTGTGGGATGAGGaaacgg                                                                                                                                               >  1:1937272/1‑147 (MQ=255)
        ccGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgt                                                                                                                                               <  2:2727091/150‑1 (MQ=255)
        ccGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgt                                                                                                                                               >  1:3365969/1‑150 (MQ=255)
        ccGGGGTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgt                                                                                                                                               <  1:173606/150‑1 (MQ=255)
           ggTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTc                                                                                                                                            <  2:543845/150‑1 (MQ=255)
               gCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTgg                                                                                                                                        <  2:194167/150‑1 (MQ=255)
                cATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGc                                                                                                                                       <  2:1364353/150‑1 (MQ=255)
                 aTTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCa                                                                                                                                      <  2:65983/150‑1 (MQ=255)
                 aTTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCa                                                                                                                                      <  2:353909/150‑1 (MQ=255)
                 aTTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGGGGGTTGCGGTTGTGTAAAGGTGTCGTGGCa                                                                                                                                      >  2:559615/1‑150 (MQ=255)
                    tGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAGGTATGGCGCGGTGGGTTTGGGATGGGGTAAGGGGtggtgggctaa                                                                                                                                   >  1:522780/1‑145 (MQ=255)
                     gCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAAc                                                                                                                                  <  2:2002861/150‑1 (MQ=255)
                       tCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGGCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAAAAg                                                                                                                                >  1:3855685/1‑150 (MQ=255)
                         caccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcg                                                                                                                              <  2:2410263/150‑1 (MQ=255)
                         caccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcg                                                                                                                              <  2:2593740/150‑1 (MQ=255)
                              gggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCa                                                                                                                         <  1:4325358/150‑1 (MQ=255)
                               ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaa                                                                                                                        <  2:2116197/150‑1 (MQ=255)
                               ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTATTATTGCGCGGGGGGGTTCGGGTGAGTAAAAGTGTCGTGGGATAAAAGCGCGGCaa                                                                                                                        >  1:4268931/1‑150 (MQ=255)
                                 ccTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                                                      <  2:1372288/150‑1 (MQ=255)
                                  cTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAacac                                                                                                                     <  1:4311758/150‑1 (MQ=255)
                                   tGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACg                                                                                                                    <  1:3293681/150‑1 (MQ=255)
                                        gCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGt                                                                                                               <  1:1285588/150‑1 (MQ=255)
                                         cGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTACGTCTGGGGCGGGGGGTTGCGGGTGTGGGAAGGGGGCGTGGGGTAACAGCGCGGCAACACGAACgga                                                                                                              >  2:4287833/1‑148 (MQ=255)
                                          gACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTAt                                                                                                             <  1:3489802/150‑1 (MQ=255)
                                            cGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTc                                                                                                           <  2:1515544/150‑1 (MQ=255)
                                                gAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCgatg                                                                                                       <  2:733289/150‑1 (MQ=255)
                                                gAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCgatg                                                                                                       <  1:2866584/150‑1 (MQ=255)
                                                gAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACATCTGGCGCGGTGGGTTGCGGGTGAGTGAAGGTGTCGGGGCATAACAGCGCGGCCACACGAACGGATTCggtg                                                                                                       >  2:2471462/1‑150 (MQ=255)
                                                gAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTAATTCTGGGGCGGGCGGGTGCGGATGAGTAAAGGGGTGGTGGCATAACAGCGCGGGCACACGCACCGAGTCgatt                                                                                                       >  2:156838/1‑149 (MQ=255)
                                                 aaaCTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTACTTATGGCGCGGGCGGTTGCGGATGAGTAAAGGGGTAGGGGCAGAAAACCGCGGCAACACCGACGGATTAgatgt                                                                                                      >  2:893420/1‑149 (MQ=255)
                                                  aaCTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCgatgat                                                                                                     <  2:3277246/150‑1 (MQ=255)
                                                  aaCTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTATTATTGGGCGGTAGGGTGCGGGTGATTAAAGGTGTCGTGGGAAAAAAGCGCGGCAACACCAACCGATTAgatgat                                                                                                     >  1:292243/1‑150 (MQ=255)
                                                   aCTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGAtc                                                                                                    <  1:2395009/150‑1 (MQ=255)
                                                   aCTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTCTGGCGCGGTAGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGAtc                                                                                                    >  2:4169562/1‑150 (MQ=255)
                                                     tctTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGAtctc                                                                                                  <  1:1152267/150‑1 (MQ=255)
                                                      ctTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATctct                                                                                                 <  2:311487/150‑1 (MQ=255)
                                                      ctTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATctct                                                                                                 <  2:311547/150‑1 (MQ=255)
                                                      ctTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTGTTTTTTCTTAGTGGGCGGTCGGGTGCGGGTGAGTTAAGGTGTGGTTGCATAACCTCGCGGCAACACCCACGTATTCgatgatagat                                                                                                 >  1:3990922/1‑146 (MQ=255)
                                                        tGCCGGGATATCTCACTCATAACACTCTCCTTCCGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTc                                                                                               <  2:3859648/150‑1 (MQ=255)
                                                         gCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCa                                                                                              <  2:3879211/150‑1 (MQ=255)
                                                         gCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCa                                                                                              <  2:849639/150‑1 (MQ=255)
                                                          ccGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCaa                                                                                             <  2:2760311/150‑1 (MQ=255)
                                                            gggATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTAGGGCGCGGTCGGTTGCGGGTGAGTAAATGTGTCGTGGCATAAAAGCGCGGCAACAACAAAGTATTAGATGATCTCTTaaatc                                                                                           >  1:1110379/1‑148 (MQ=255)
                                                             ggATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCg                                                                                          <  2:3964852/150‑1 (MQ=255)
                                                             ggATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCg                                                                                          <  1:2854999/150‑1 (MQ=255)
                                                             ggATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCg                                                                                          <  2:2218008/150‑1 (MQ=255)
                                                               atatCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTATTGTGGGGGGGGCGGTTGGGGGTGGGTAAAGGTGGGGTGGCATAAAAGCGCGGCCACACCAAAGTATTAGATGATATATTCAAGCGAc                                                                                        >  1:2785011/1‑150 (MQ=255)
                                                                   ctcactcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACAttt                                                                                    <  2:2852450/150‑1 (MQ=255)
                                                                   ctcactcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTCTGGCGCGGGGGGTTGCGGGTGAGTAAATGTGTCGTGGCATAACAGCGCGGCAACACCAACGGAATAGATGATATATTCAATCCACAatt                                                                                    >  1:737879/1‑150 (MQ=255)
                                                                     cactcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCa                                                                                  <  1:4046064/150‑1 (MQ=255)
                                                                      actcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCaa                                                                                 <  1:4612275/150‑1 (MQ=255)
                                                                       ctcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAg                                                                                <  1:1461522/150‑1 (MQ=255)
                                                                        tcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGc                                                                               <  1:2317325/150‑1 (MQ=255)
                                                                         caTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTCTTGCGCGGTCGGTTGCGGGTGGGTAAAGGTGTGGGGGCCATACAACGCGGCCACACAAACGTATTGGATGTTAGATTAAAGCGACAATTAAAGcg                                                                              >  1:626300/1‑149 (MQ=255)
                                                                            aaCACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCtt                                                                           <  1:2714420/150‑1 (MQ=255)
                                                                             acacTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTc                                                                          <  1:3428289/150‑1 (MQ=255)
                                                                               acTCTCCTTACTTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCt                                                                        <  1:3512507/150‑1 (MQ=255)
                                                                               acTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCt                                                                        <  1:1025791/150‑1 (MQ=255)
                                                                                ctctCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTATGGCGCGGTCGGGTGGGGGTGTGTTAATGTGTAGTGGCATAACAGCGCGGCAACACGGACGTATTGGATTTTGTCTTCAAGCGCCCATTCAAGCTCttacgt                                                                       >  2:460941/1‑146 (MQ=255)
                                                                                  ctcCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAt                                                                     <  2:3799353/150‑1 (MQ=255)
                                                                                  ctcCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAt                                                                     <  2:3575166/150‑1 (MQ=255)
                                                                                   tcCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTCTGGCGCGGTCGGTTTCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGGATTAGATGATATCATCAAGCGACAATTAAAGCTCTTACAGAtc                                                                    >  2:3059581/1‑150 (MQ=255)
                                                                                   tcCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTATGGCGCGGTCGGTTGCGGATGTGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACCAACCTATTCGATGATCACTTAAAGCGACATTTAAAGCTCTTACTGAtc                                                                    >  2:581264/1‑150 (MQ=255)
                                                                                     cTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtctt                                                                  <  1:2951609/150‑1 (MQ=255)
                                                                                           ttttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt                                                            <  1:2401029/150‑1 (MQ=255)
                                                                                            tttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                                                           <  2:1051779/150‑1 (MQ=255)
                                                                                             ttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttcg                                                          <  2:1876083/150‑1 (MQ=255)
                                                                                              tttGTTTTTAGTGTAGAGTCGGTTTTTGTAATTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                                         <  1:3364295/150‑1 (MQ=255)
                                                                                                  tttttAGTGTAGAGTCGGTTTTTTTAATTATGGGGCGGTAGGTTTCGGATGTGTAAAGGTGTGGTTGCATAAAAGCGCGGCAAAAAGAACGGATTAGATGATCTATTAAAGCGACAATTAAAGCTCTTAATGATATTCGTGTTAGTCGTa                                                     >  2:484318/1‑150 (MQ=255)
                                                                                                  tttttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTa                                                     <  2:337498/150‑1 (MQ=255)
                                                                                                        gtgtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAg                                               <  2:164718/150‑1 (MQ=255)
                                                                                                         tgtAGAGTCGGTTTTTTTACATAAGGCGCGGGCGGTTTGGGATGAGTAAAGGTGTCGTGGCAGAAAAACGCCGCCACACCAACGTTTTATTTGATATCTTCAAGAGACATTTAAAGCACATACAGATCATCGTGTTCGTCGGAACCCAga                                              >  2:113024/1‑149 (MQ=255)
                                                                                                           tAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTg                                            <  2:4082317/150‑1 (MQ=255)
                                                                                                              agTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTgcgc                                         >  1:2257024/1‑150 (MQ=255)
                                                                                                              agTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTgcgc                                         <  2:1985314/150‑1 (MQ=255)
                                                                                                              agTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTgcgc                                         <  2:1435459/150‑1 (MQ=255)
                                                                                                                tCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCaa                                       >  1:3720918/1‑150 (MQ=255)
                                                                                                                tCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCaa                                       >  1:3720945/1‑150 (MQ=255)
                                                                                                                  ggTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATg                                     >  2:1817801/1‑150 (MQ=255)
                                                                                                                              tCTGGCGCTGTCGGTTGCGGTTGAGTAAAGGTGTCGTGTCATAACAGCGCGGCAACAAGATCTTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtcg                         <  1:4499488/150‑1 (MQ=255)
                                                                                                                              tCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtcg                         >  2:4239091/1‑150 (MQ=255)
                                                                                                                                   cgcgGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGAt                    <  2:1389015/150‑1 (MQ=255)
                                                                                                                                   cgcgGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGAt                    <  2:2417122/150‑1 (MQ=255)
                                                                                                                                    gcgGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATa                   >  2:4485070/1‑150 (MQ=255)
                                                                                                                                      ggtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGc                 >  1:3964964/1‑150 (MQ=255)
                                                                                                                                        tcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCtt               <  1:1348021/150‑1 (MQ=255)
                                                                                                                                        tcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCtt               <  1:1844016/150‑1 (MQ=255)
                                                                                                                                          ggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCa             >  1:2096809/1‑150 (MQ=255)
                                                                                                                                          ggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCa             >  2:3736802/1‑150 (MQ=255)
                                                                                                                                            ttGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCAcc           <  1:1715698/150‑1 (MQ=255)
                                                                                                                                               cGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGt        >  2:3009211/1‑150 (MQ=255)
                                                                                                                                                ggATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGtt       <  2:1911116/150‑1 (MQ=255)
                                                                                                                                                 gATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGttt      <  2:1105812/150‑1 (MQ=255)
                                                                                                                                                 gATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGttt      <  2:2785011/150‑1 (MQ=255)
                                                                                                                                                  aTGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTc     <  1:546336/150‑1 (MQ=255)
                                                                                                                                                     aGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGcc  >  1:2508664/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCC  >  CP009273/3048124‑3048422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑