BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,048,490	A→T	10.2%	L105* (TTG→TAG)	ygfB ←	UPF0149 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,048,490	0	A	T	10.2%	934.5 / 77.9	299	L105* (TTG→TAG)	ygfB	UPF0149 family protein
Reads supporting (aligned to +/- strand): ref base A (145/123); new base T (20/11); total (165/134)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

CAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCG  >  CP009273/3048341‑3048639

cAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCa                                                                                                                                                       <  2:199985/150‑1 (MQ=255)

cAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTGTCACCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCa                                                                                                                                                       <  2:4135642/150‑1 (MQ=255)

  aGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAAt                                                                                                                                                     <  1:3521328/150‑1 (MQ=255)

  aGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAAt                                                                                                                                                     <  2:1464918/150‑1 (MQ=255)

  aGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAAt                                                                                                                                                     <  2:2568898/150‑1 (MQ=255)

   gCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATg                                                                                                                                                    >  2:2826175/1‑150 (MQ=255)

     tctTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCa                                                                                                                                                  <  2:4268931/150‑1 (MQ=255)

      ctTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCAt                                                                                                                                                 >  1:703180/1‑150 (MQ=255)

      ctTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTACGCCGGACACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCAGCCTATGCAt                                                                                                                                                 >  1:3326797/1‑150 (MQ=255)

       ttCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATc                                                                                                                                                >  1:2872027/1‑150 (MQ=255)

       ttCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCGTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATc                                                                                                                                                <  2:685123/150‑1 (MQ=255)

            gATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGccc                                                                                                                                           <  2:2809950/150‑1 (MQ=255)

               cttcgtcttcgtCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGAt                                                                                                                                        <  1:2095769/150‑1 (MQ=255)

               cttcgtcttcgtCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGAt                                                                                                                                        >  1:2160936/1‑150 (MQ=255)

               cttcgtcttcgtCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGAt                                                                                                                                        <  1:715079/150‑1 (MQ=255)

                ttcgtcttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATc                                                                                                                                       <  1:584359/150‑1 (MQ=255)

                   gtcttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGaa                                                                                                                                    >  1:1645533/1‑150 (MQ=255)

                   gtcttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGaa                                                                                                                                    >  2:1056924/1‑150 (MQ=255)

                      ttcgtcGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAAc                                                                                                                                 <  2:3688637/150‑1 (MQ=255)

                       tcgtcgTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGATACACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGTAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACg                                                                                                                                >  2:901913/1‑150 (MQ=255)

                         gtcgtAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCt                                                                                                                              >  1:818720/1‑150 (MQ=255)

                         gtcgtAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTACCAATGCATCAGCCCGATCGAAAACGCt                                                                                                                              >  1:4156841/1‑150 (MQ=255)

                           cgtAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGa                                                                                                                            >  1:516383/1‑150 (MQ=255)

                                cccAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGAcatca                                                                                                                       >  1:2075245/1‑150 (MQ=255)

                                   aGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCg                                                                                                                    >  1:663599/1‑150 (MQ=255)

                                    gTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGc                                                                                                                   <  1:1971024/150‑1 (MQ=255)

                                     ttGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGcc                                                                                                                  <  2:3990922/150‑1 (MQ=255)

                                      tGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCa                                                                                                                 <  2:2257024/150‑1 (MQ=255)

                                        cgcAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATc                                                                                                               >  1:639425/1‑150 (MQ=255)

                                        cgcAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATc                                                                                                               >  1:3486775/1‑150 (MQ=255)

                                         gcAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCa                                                                                                              <  1:3059581/150‑1 (MQ=255)

                                          cAAAGTTACGCAGATCGTCGATATCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTAGCGTAACGCCAAGACCAAGCAGGAAGTTATTGACCCAACCTGCCAATGCATCACCCCGAACGAAAACGCTGACAACATCGCCATCAg                                                                                                             >  2:2092865/1‑150 (MQ=255)

                                           aaTGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAgg                                                                                                            >  1:1697798/1‑150 (MQ=255)

                                           aaTGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAgg                                                                                                            >  1:3676524/1‑150 (MQ=255)

                                                  cGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATaa                                                                                                     >  2:1817870/1‑150 (MQ=255)

                                                     aGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGc                                                                                                  >  2:3127644/1‑150 (MQ=255)

                                                     aGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGc                                                                                                  >  2:4407131/1‑150 (MQ=255)

                                                      gATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCACTGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCt                                                                                                 <  1:3993943/150‑1 (MQ=255)

                                                        tcgtcgATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGa                                                                                               <  1:4258272/150‑1 (MQ=255)

                                                         cgtcgATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGaa                                                                                              >  1:1072577/1‑150 (MQ=255)

                                                            cgATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGaaaaa                                                                                           >  2:984900/1‑150 (MQ=255)

                                                            cgATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGaaaaa                                                                                           >  1:3240296/1‑150 (MQ=255)

                                                               tAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATTGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGa                                                                                        >  1:3477270/1‑150 (MQ=255)

                                                               tAGCATCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGa                                                                                        >  1:3084141/1‑150 (MQ=255)

                                                                aGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGaa                                                                                       <  2:4085185/150‑1 (MQ=255)

                                                                 gCTTCACCGGTGTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAg                                                                                      <  1:1563940/150‑1 (MQ=255)

                                                                  cTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGNCATCAGGCAGATAAAGCTGAAAAAGGAAGc                                                                                     <  1:4287833/150‑1 (MQ=255)

                                                                   ttCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGcc                                                                                    <  2:2762503/150‑1 (MQ=255)

                                                                    tCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCg                                                                                   <  1:4239091/150‑1 (MQ=255)

                                                                          gTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATcc                                                                             >  1:1588816/1‑150 (MQ=255)

                                                                             tCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGc                                                                          >  2:3713490/1‑150 (MQ=255)

                                                                              cGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCa                                                                         >  2:3508882/1‑150 (MQ=255)

                                                                              cGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCa                                                                         >  2:1201499/1‑150 (MQ=255)

                                                                               gCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAg                                                                        >  1:2413686/1‑150 (MQ=255)

                                                                                 cGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAggg                                                                      >  1:1205045/1‑150 (MQ=255)

                                                                                  ggTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGc                                                                     >  1:1027084/1‑150 (MQ=255)

                                                                                  ggTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGc                                                                     >  1:1584177/1‑150 (MQ=255)

                                                                                  ggTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGc                                                                     >  1:4380572/1‑150 (MQ=255)

                                                                                     cACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATc                                                                  <  1:313778/150‑1 (MQ=255)

                                                                                     cACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATc                                                                  <  2:3370728/150‑1 (MQ=255)

                                                                                     cACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATc                                                                  <  1:2269682/150‑1 (MQ=255)

                                                                                       cTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGc                                                                <  1:1261240/150‑1 (MQ=255)

                                                                                       cTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGc                                                                <  1:2651285/150‑1 (MQ=255)

                                                                                        tttATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCt                                                               <  2:601772/150‑1 (MQ=255)

                                                                                          tATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCtgg                                                             <  1:4376405/150‑1 (MQ=255)

                                                                                                gCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCag                                                       >  1:4063521/1‑150 (MQ=255)

                                                                                                gCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCATCAGCCCGAGCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCag                                                       >  1:3829917/1‑150 (MQ=255)

                                                                                                 cTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCaga                                                      >  1:391139/1‑150 (MQ=255)

                                                                                                  ttCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCagag                                                     <  1:297573/150‑1 (MQ=255)

                                                                                                  ttCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCagag                                                     <  1:4566826/150‑1 (MQ=255)

                                                                                                      gTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCa                                                 >  1:2905955/1‑150 (MQ=255)

                                                                                                            tAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTAc                                           >  1:3681986/1‑150 (MQ=255)

                                                                                                            tAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTAc                                           <  1:1817801/150‑1 (MQ=255)

                                                                                                               cGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCa                                        <  1:113024/150‑1 (MQ=255)

                                                                                                                gCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAg                                       >  2:4534122/1‑150 (MQ=255)

                                                                                                                 ccAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCTATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGt                                      <  1:4485070/150‑1 (MQ=255)

                                                                                                                 ccAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGt                                      >  2:1370704/1‑150 (MQ=255)

                                                                                                                  cAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTg                                     >  2:4177547/1‑150 (MQ=255)

                                                                                                                   aaGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGc                                    >  1:1308110/1‑150 (MQ=255)

                                                                                                                   aaGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGc                                    <  1:2615497/150‑1 (MQ=255)

                                                                                                                    aGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGcc                                   >  1:1361605/1‑150 (MQ=255)

                                                                                                                     gACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCt                                  <  2:1638383/150‑1 (MQ=255)

                                                                                                                      aCCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCtg                                 >  2:631819/1‑150 (MQ=255)

                                                                                                                       ccAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCtgt                                <  2:1408063/150‑1 (MQ=255)

                                                                                                                        cAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCtgtg                               <  2:2160418/150‑1 (MQ=255)

                                                                                                                        cAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCtgtg                               >  1:1834291/1‑150 (MQ=255)

                                                                                                                          aGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGcc                             >  2:3966605/1‑150 (MQ=255)

                                                                                                                              ggAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCATAGTGCATTTTACGCAGTGCCTGTGCCAGCt                         <  2:1510895/150‑1 (MQ=255)

                                                                                                                              ggAAGTGATTGACCCAACCTGCCAATGCATCAGCCAGATCGATTACTCTGACATCATCGCCATCAGGCAGATAAAGCTTAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCt                         <  2:3720944/150‑1 (MQ=255)

                                                                                                                                 aGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCAt                      >  1:3409138/1‑150 (MQ=255)

                                                                                                                                   tGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGa                    <  2:1292103/150‑1 (MQ=255)

                                                                                                                                     aTTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGAcc                  <  2:3890177/150‑1 (MQ=255)

                                                                                                                                     aTTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGAcc                  <  1:2239494/150‑1 (MQ=255)

                                                                                                                                       tGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGa                >  2:2412270/1‑150 (MQ=255)

                                                                                                                                            cAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGcc           >  2:251776/1‑150 (MQ=255)

                                                                                                                                              aCCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCAt         <  2:2905868/150‑1 (MQ=255)

                                                                                                                                                 tGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGcc      >  2:3779881/1‑150 (MQ=255)

                                                                                                                                                    cAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCttc   <  2:3263714/150‑1 (MQ=255)

                                                                                                                                                    cAATGCATCAGCCCGATAGAAAACGCTGACCTCCTCGCATTCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCttc   <  1:901913/150‑1 (MQ=255)

                                                                                                                                                     aaTGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCttcg  >  1:523325/1‑150 (MQ=255)

CAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCACTTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCG  >  CP009273/3048341‑3048639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`