Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,065,300 C→A 15.8% D228Y (GAT→TAT)  pgk ← phosphoglycerate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,065,3000CA15.8% 927.1 / 95.5 284D228Y (GAT→TAT) pgkphosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base C (133/105);  new base A (27/19);  total (160/124)
Fisher's exact test for biased strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

CACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGTTCTT  >  CP009273/3065151‑3065446
                                                                                                                                                     |                                                                                                                                                  
cacaTCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATc                                                                                                                                                    >  1:2995640/1‑150 (MQ=255)
  caTCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGt                                                                                                                                                  >  1:387389/1‑150 (MQ=255)
      gTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGcc                                                                                                                                              <  1:967636/150‑1 (MQ=255)
      gTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGcc                                                                                                                                              <  1:1646070/150‑1 (MQ=255)
       ttAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCt                                                                                                                                             <  2:2459542/150‑1 (MQ=255)
          aCAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTtgt                                                                                                                                          >  1:1289895/1‑150 (MQ=255)
          aCAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTtgt                                                                                                                                          >  2:1707510/1‑150 (MQ=255)
           cAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTtgtg                                                                                                                                         <  2:2499529/150‑1 (MQ=255)
            aGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGc                                                                                                                                        <  2:297997/150‑1 (MQ=255)
                ttCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcgcg                                                                                                                                    <  1:3004506/150‑1 (MQ=255)
                ttCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcgcg                                                                                                                                    >  2:2660926/1‑150 (MQ=255)
                ttCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcgcg                                                                                                                                    <  1:558080/150‑1 (MQ=255)
                 tCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGa                                                                                                                                   >  1:52360/1‑150 (MQ=255)
                 tCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACAGCGTGGCCTTGTGCCGCGa                                                                                                                                   <  2:3576011/150‑1 (MQ=255)
                   aGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATa                                                                                                                                 >  1:4509682/1‑150 (MQ=255)
                      gTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAg                                                                                                                              <  1:2416029/150‑1 (MQ=255)
                       tAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAgg                                                                                                                             <  2:1329799/150‑1 (MQ=255)
                       tAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAgg                                                                                                                             >  2:1126471/1‑150 (MQ=255)
                          ccGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGgtgt                                                                                                                          >  1:4109050/1‑150 (MQ=255)
                           cGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGtt                                                                                                                         <  2:2742201/150‑1 (MQ=255)
                           cGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGtt                                                                                                                         >  2:3091412/1‑150 (MQ=255)
                           aggTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGTTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAATgtgta                                                                                                                         >  2:939453/2‑149 (MQ=255)
                            ggTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTa                                                                                                                        >  2:3923571/1‑150 (MQ=255)
                             gTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAg                                                                                                                       >  1:249126/1‑150 (MQ=255)
                             gTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAg                                                                                                                       >  2:3908062/1‑150 (MQ=255)
                                 aGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGAt                                                                                                                   <  1:2930760/150‑1 (MQ=255)
                                 aGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGAt                                                                                                                   <  1:1111185/150‑1 (MQ=255)
                                    ttCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATacc                                                                                                                <  1:1457229/150‑1 (MQ=255)
                                     tCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATacca                                                                                                               >  1:3105955/1‑150 (MQ=255)
                                        gagaACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATaccacca                                                                                                            <  2:279995/150‑1 (MQ=255)
                                               cGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACcaacaa                                                                                                     >  1:3725059/1‑150 (MQ=255)
                                                ggTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAAt                                                                                                    <  1:3307982/150‑1 (MQ=255)
                                                  ttGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCa                                                                                                  <  2:2876217/150‑1 (MQ=255)
                                                      tACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTg                                                                                              >  1:1067353/1‑150 (MQ=255)
                                                         gcgAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTc                                                                                           <  2:2757014/150‑1 (MQ=255)
                                                             aCATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCg                                                                                       >  1:3413865/1‑150 (MQ=255)
                                                             aCATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCg                                                                                       <  2:3717301/150‑1 (MQ=255)
                                                              cATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGa                                                                                      >  1:3608144/1‑150 (MQ=255)
                                                              cATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGa                                                                                      <  2:3806381/150‑1 (MQ=255)
                                                              cATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGa                                                                                      >  1:4133824/1‑150 (MQ=255)
                                                              cATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGa                                                                                      >  1:2303390/1‑150 (MQ=255)
                                                               aTCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAt                                                                                     <  2:4458494/150‑1 (MQ=255)
                                                                tCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAtt                                                                                    <  2:3149212/150‑1 (MQ=255)
                                                                tCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAtt                                                                                    >  1:3284761/1‑150 (MQ=255)
                                                                   gACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTa                                                                                 >  1:2376783/1‑150 (MQ=255)
                                                                        aaCCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAg                                                                            >  1:3366496/1‑150 (MQ=255)
                                                                           cGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGa                                                                         >  2:193499/1‑150 (MQ=255)
                                                                              gATGTTGCAGGTGGTCAGCAGACGTTTAGCTGCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTc                                                                      >  2:2259114/1‑150 (MQ=255)
                                                                                 gTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAg                                                                   >  2:1523458/1‑150 (MQ=255)
                                                                                    gCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAAc                                                                <  1:742327/150‑1 (MQ=255)
                                                                                      aGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACgg                                                              <  1:370106/150‑1 (MQ=255)
                                                                                      aGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACgg                                                              >  1:726947/1‑150 (MQ=255)
                                                                                         tggtCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCa                                                           <  1:1427858/150‑1 (MQ=255)
                                                                                         tggtCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCa                                                           <  2:3731743/150‑1 (MQ=255)
                                                                                         tggtCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCa                                                           <  2:1234653/150‑1 (MQ=255)
                                                                                         tggtCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCANCGATTTTAGACAGGGAGTCCAGAACGGTCa                                                           <  1:4284464/150‑1 (MQ=255)
                                                                                           gtCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGt                                                         <  1:2221432/150‑1 (MQ=255)
                                                                                            tCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGtt                                                        >  1:2514866/1‑150 (MQ=255)
                                                                                             cagcagACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGttt                                                       >  2:3727268/1‑150 (MQ=255)
                                                                                              agcagACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTg                                                      <  2:1398521/150‑1 (MQ=255)
                                                                                                cagACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGt                                                    >  1:1866410/1‑150 (MQ=255)
                                                                                                    cGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCTATACCACCACCAACAATCAGCTGGTCAGCGATATTAGACAGTGAGTCCAGAACGGTCAGTTTGGTAGAt                                                >  1:1812822/1‑150 (MQ=255)
                                                                                                    cGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGAt                                                >  2:736660/1‑150 (MQ=255)
                                                                                                     gTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCTGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATa                                               >  1:2134934/1‑150 (MQ=255)
                                                                                                     gTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCCGAACGGTCAGTTTGGTAGATa                                               >  1:4348129/1‑150 (MQ=255)
                                                                                                      tttAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATAc                                              <  1:1616562/150‑1 (MQ=255)
                                                                                                      tttAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATAc                                              <  2:2783838/150‑1 (MQ=255)
                                                                                                           cTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAg                                         >  2:454265/1‑150 (MQ=255)
                                                                                                               gTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAacc                                     >  2:1450547/1‑150 (MQ=255)
                                                                                                                 cAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAaccac                                   >  2:918088/1‑150 (MQ=255)
                                                                                                                 cAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAaccac                                   >  2:723927/1‑150 (MQ=255)
                                                                                                                  aaCCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAaccacc                                  >  1:2585713/1‑150 (MQ=255)
                                                                                                                   aCCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAAccacca                                 <  1:28222/150‑1 (MQ=255)
                                                                                                                    ccAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCaa                                >  1:1034271/1‑150 (MQ=255)
                                                                                                                         tCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATa                           >  1:2767765/1‑150 (MQ=255)
                                                                                                                         tCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATa                           >  1:3921681/1‑150 (MQ=255)
                                                                                                                           aGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGc                         >  1:2050581/1‑150 (MQ=255)
                                                                                                                           aGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGc                         <  1:1712393/150‑1 (MQ=255)
                                                                                                                            gCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGcc                        >  1:83780/1‑150 (MQ=255)
                                                                                                                              ttCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGccac                      >  2:2100857/1‑150 (MQ=255)
                                                                                                                              ttCGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGccac                      >  1:459336/1‑150 (MQ=255)
                                                                                                                                cGTACAGGGATTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGccacca                    >  1:1969358/1‑150 (MQ=255)
                                                                                                                                 gTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCAt                   >  2:3439535/1‑150 (MQ=255)
                                                                                                                                  tACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATc                  >  2:534642/1‑150 (MQ=255)
                                                                                                                                   aCAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCg                 <  2:1849864/150‑1 (MQ=255)
                                                                                                                                    cAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCgg                >  2:1975210/1‑150 (MQ=255)
                                                                                                                                     aGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCggg               <  1:119016/150‑1 (MQ=255)
                                                                                                                                      gggATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGc              <  2:1987477/150‑1 (MQ=255)
                                                                                                                                      gggATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCAACACCAACAAACATCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGTTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGc              >  1:1757041/1‑150 (MQ=255)
                                                                                                                                         aTTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAg           >  2:4075085/1‑150 (MQ=255)
                                                                                                                                         aTTTACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAg           >  2:2838606/1‑150 (MQ=255)
                                                                                                                                            tACCCACATAGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAg        <  2:586341/150‑1 (MQ=255)
                                                                                                                                             aCCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAgg       >  2:1838015/1‑150 (MQ=255)
                                                                                                                                             aCCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACGTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAgg       >  2:233723/1‑150 (MQ=255)
                                                                                                                                              cccACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGt      >  2:4274868/1‑150 (MQ=255)
                                                                                                                                              cccACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGt      <  1:2660926/150‑1 (MQ=255)
                                                                                                                                               ccACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGtt     >  2:3534004/1‑150 (MQ=255)
                                                                                                                                               ccACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGtt     <  1:2041271/150‑1 (MQ=255)
                                                                                                                                               ccACATCGTGGCCTTGTGCCACGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGtt     <  1:1323028/150‑1 (MQ=255)
                                                                                                                                                  caTCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGTTCtt  >  1:4028396/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGTCCAGAACGGTCAGTTTGGTAGATACTTTAGAACCACCAACGATAGCCACCATCGGGCGAGCAGGTTCTT  >  CP009273/3065151‑3065446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑