Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,076,526 C→A 100% D211Y (GAC→TAC)  speB ← agmatinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,076,5260CA97.1% 909.4 / 15.9 274D211Y (GAC→TAC) speBagmatinase
Reads supporting (aligned to +/- strand):  ref base C (3/5);  new base A (130/135);  total (133/141)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 274 total aligned reads displayed.

GTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGT  >  CP009273/3076379‑3076672
                                                                                                                                                   |                                                                                                                                                  
gTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAAt                                                                                                                                                  <  2:948389/150‑1 (MQ=255)
gTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAAt                                                                                                                                                  >  2:3233369/1‑150 (MQ=255)
gTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAAt                                                                                                                                                  >  2:1455646/1‑150 (MQ=255)
   ccAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCa                                                                                                                                               <  2:554589/150‑1 (MQ=255)
   ccAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCa                                                                                                                                               <  2:3004890/150‑1 (MQ=255)
    cAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCAc                                                                                                                                              <  2:1267822/150‑1 (MQ=255)
    cAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCAc                                                                                                                                              <  2:1393752/150‑1 (MQ=255)
     aGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACg                                                                                                                                             >  2:392346/1‑150 (MQ=255)
       tttAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCt                                                                                                                                           <  1:2360034/150‑1 (MQ=255)
       tttAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCt                                                                                                                                           >  2:4598498/1‑150 (MQ=255)
        ttAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTg                                                                                                                                          <  1:72749/150‑1 (MQ=255)
         tAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGc                                                                                                                                         >  2:3307454/1‑150 (MQ=255)
          aaTAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCg                                                                                                                                        <  1:2448380/150‑1 (MQ=255)
          aaTAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCg                                                                                                                                        <  2:3090239/150‑1 (MQ=255)
             aGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATc                                                                                                                                     <  1:3659858/150‑1 (MQ=255)
                 cgATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTc                                                                                                                                 >  2:2532010/1‑150 (MQ=255)
                  gATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCa                                                                                                                                >  2:1768321/1‑150 (MQ=255)
                  gATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCa                                                                                                                                <  2:464113/150‑1 (MQ=255)
                        aGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGc                                                                                                                          <  2:153115/150‑1 (MQ=255)
                         ggTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCa                                                                                                                         >  1:4409832/1‑150 (MQ=255)
                         ggTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCa                                                                                                                         <  2:3741664/150‑1 (MQ=255)
                          gTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAg                                                                                                                        <  1:4225529/150‑1 (MQ=255)
                            cAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGc                                                                                                                      >  1:207876/1‑150 (MQ=255)
                             aGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCg                                                                                                                     <  2:3439123/150‑1 (MQ=255)
                              ggCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGt                                                                                                                    >  2:3183026/1‑150 (MQ=255)
                              ggCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGt                                                                                                                    <  1:1823507/150‑1 (MQ=255)
                                    cAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCa                                                                                                              >  2:619969/1‑150 (MQ=255)
                                    cAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCa                                                                                                              >  2:2124567/1‑150 (MQ=255)
                                        cACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGt                                                                                                          <  2:925323/150‑1 (MQ=255)
                                           tGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCAAATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTaaa                                                                                                       >  1:3859607/1‑150 (MQ=255)
                                            ggCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAg                                                                                                      >  2:3694639/1‑150 (MQ=255)
                                             gCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGc                                                                                                     <  2:2906015/150‑1 (MQ=255)
                                               gTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCg                                                                                                   >  1:1258906/1‑150 (MQ=255)
                                                 aCCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGtt                                                                                                 >  2:233762/1‑150 (MQ=255)
                                                          tGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATc                                                                                        <  1:997896/150‑1 (MQ=255)
                                                          tGGTGAAAAAGAATGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATc                                                                                        <  1:613431/150‑1 (MQ=255)
                                                           ggTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCa                                                                                       >  1:956151/1‑150 (MQ=255)
                                                           ggTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCa                                                                                       <  1:105779/150‑1 (MQ=255)
                                                            gTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCaa                                                                                      <  1:1551039/150‑1 (MQ=255)
                                                             tGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCaaa                                                                                     >  1:1759390/1‑150 (MQ=255)
                                                             tGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCaaa                                                                                     >  1:3016075/1‑150 (MQ=255)
                                                                 aaaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCg                                                                                 <  2:2170138/150‑1 (MQ=255)
                                                                   aGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGt                                                                               >  2:1903485/1‑150 (MQ=255)
                                                                   aGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGt                                                                               <  1:2689970/150‑1 (MQ=255)
                                                                    gCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTa                                                                              <  1:4098875/150‑1 (MQ=255)
                                                                    gCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTa                                                                              <  2:1898005/150‑1 (MQ=255)
                                                                     cAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTAc                                                                             <  1:1502133/150‑1 (MQ=255)
                                                                       ggATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGa                                                                           <  1:1273113/150‑1 (MQ=255)
                                                                          tCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATa                                                                        >  2:4576397/1‑150 (MQ=255)
                                                                                  gTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTg                                                                <  1:1996897/150‑1 (MQ=255)
                                                                                    cGATATCAAATGTCATGTAATCCGTAATATCACCCAAAATCTGTTTGACTTGGGCGATAACGTAATCAACTCTGCGATCGTTCACCTGGCAGGCGTCTTGCTGGTTAAAGCCGTTTTCTTTATCAAACTCGGTTCGTATACCAATCTGCa                                                              <  1:431064/150‑1 (MQ=255)
                                                                                    cGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTAGTCTTTATCAAACTCGGTACGAATACCAAACTGCa                                                              >  1:3180100/1‑150 (MQ=255)
                                                                                     gATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCAc                                                             <  2:2595345/150‑1 (MQ=255)
                                                                                     gATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCAc                                                             <  2:153545/150‑1 (MQ=255)
                                                                                         tCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGaa                                                         >  1:1358656/1‑150 (MQ=255)
                                                                                           aaaaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATg                                                       <  2:2728571/150‑1 (MQ=255)
                                                                                           aaaaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATg                                                       <  1:3290611/150‑1 (MQ=255)
                                                                                           aaaaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATg                                                       <  2:3592691/150‑1 (MQ=255)
                                                                                             aaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAt                                                     >  2:4209345/1‑150 (MQ=255)
                                                                                             aaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAt                                                     >  1:112048/1‑150 (MQ=255)
                                                                                             aaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAt                                                     >  1:2227229/1‑150 (MQ=255)
                                                                                              aGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAtt                                                    <  1:1147910/150‑1 (MQ=255)
                                                                                              aGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAtt                                                    <  1:3407932/150‑1 (MQ=255)
                                                                                              aGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAtt                                                    <  1:680933/150‑1 (MQ=255)
                                                                                               gTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTc                                                   <  1:810057/150‑1 (MQ=255)
                                                                                                tCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCg                                                  <  1:926659/150‑1 (MQ=255)
                                                                                                  aGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCggg                                                >  1:1873734/1‑150 (MQ=255)
                                                                                                     tAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCg                                             >  1:3644208/1‑150 (MQ=255)
                                                                                                      aaaCCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGa                                            >  2:2326337/1‑150 (MQ=255)
                                                                                                      aaaCCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGa                                            >  2:4471050/1‑150 (MQ=255)
                                                                                                          cGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAg                                        >  1:1764038/1‑150 (MQ=255)
                                                                                                          cGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAg                                        <  1:3383738/150‑1 (MQ=255)
                                                                                                           ggCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCCATCTGCACGGACTGATTCGGGTCGATCAGa                                       >  1:4540470/1‑150 (MQ=255)
                                                                                                           ggCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGa                                       >  1:2317353/1‑150 (MQ=255)
                                                                                                           ggCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGa                                       <  1:3218981/150‑1 (MQ=255)
                                                                                                             cATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGAcc                                     >  2:247793/1‑150 (MQ=255)
                                                                                                              atatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACct                                    <  2:4625679/150‑1 (MQ=255)
                                                                                                               tatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACctt                                   >  1:795863/1‑150 (MQ=255)
                                                                                                               tatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACctt                                   <  2:4435390/150‑1 (MQ=255)
                                                                                                               tatCACCCACAATCGGTTTCACTTGGGCGATAACGTAATCCTCGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTGTTTATCAAACTCGGTACGTATACCACTCTGCACGGAATGATTCGGGTCGATCAGACctt                                   <  2:704109/150‑1 (MQ=255)
                                                                                                                atCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACcttc                                  <  2:4320060/150‑1 (MQ=255)
                                                                                                                 tCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACcttct                                 >  2:205586/1‑150 (MQ=255)
                                                                                                                  cACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACcttctt                                >  2:4283273/1‑150 (MQ=255)
                                                                                                                  cACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACcttctt                                <  2:2424977/150‑1 (MQ=255)
                                                                                                                     ccACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCg                             >  2:665798/1‑150 (MQ=255)
                                                                                                                      cacaATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCgg                            >  1:3555743/1‑150 (MQ=255)
                                                                                                                      cacaATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCgg                            <  2:1486814/150‑1 (MQ=255)
                                                                                                                         aaTCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGgcgc                         >  1:2840681/1‑150 (MQ=255)
                                                                                                                          aTCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGcgcg                        <  1:4598495/150‑1 (MQ=255)
                                                                                                                          aTCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGcgcg                        >  2:1290219/1‑150 (MQ=255)
                                                                                                                           tCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCgg                       >  2:4183946/1‑150 (MQ=255)
                                                                                                                           tCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCgg                       >  2:1080451/1‑150 (MQ=255)
                                                                                                                                  cACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGaa                <  1:3233369/150‑1 (MQ=255)
                                                                                                                                  cACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGaa                <  1:1455646/150‑1 (MQ=255)
                                                                                                                                    cTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACa              >  2:3668858/1‑150 (MQ=255)
                                                                                                                                      tGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATa            <  1:399995/150‑1 (MQ=255)
                                                                                                                                         gCGATAACGTTATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTTCACGGAATGATTCGGGTCGATCAGTCCTTCTTTCGGCGCGGTATAGAACATAGTg         <  2:2256234/150‑1 (MQ=255)
                                                                                                                                          cGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTATTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGc        >  1:3499514/1‑150 (MQ=255)
                                                                                                                                           gATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGcc       <  2:1698308/150‑1 (MQ=255)
                                                                                                                                           gATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGcc       >  2:2043267/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGT  >  CP009273/3076379‑3076672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑