BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,085,649	C→A	14.2%	T138K (ACG→AAG)	gshB →	glutathione synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,085,649	0	C	A	14.2%	1017.5 / 95.1	300	T138K (ACG→AAG)	gshB	glutathione synthetase
Reads supporting (aligned to +/- strand): ref base C (129/128); new base A (13/30); total (142/158)
Fisher's exact test for biased strand distribution p-value = 2.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 301 total aligned reads displayed.

CCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCCTG  >  CP009273/3085500‑3085797

     cgTTAGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAAAAAGCCGCAGAGCCTGCGCTAAGGTAACGAGAAACTGTTTACCGCCTGGTTCTCAGACTAAACGCCAGAAACGCggg                                                                                                                                                 >  1:151241/1‑150 (MQ=255)

           aTACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTAcgc                                                                                                                                           >  2:563772/1‑150 (MQ=255)

           aTACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTAcgc                                                                                                                                           >  1:2955474/1‑150 (MQ=255)

             aCCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACgcgc                                                                                                                                         >  1:1607826/1‑150 (MQ=255)

             aCCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACgcgc                                                                                                                                         >  1:2267611/1‑150 (MQ=255)

              ccGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCa                                                                                                                                        <  2:2679257/150‑1 (MQ=255)

               cGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCaa                                                                                                                                       >  1:4177558/1‑150 (MQ=255)

               cGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCaa                                                                                                                                       <  1:826710/150‑1 (MQ=255)

               cGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCaa                                                                                                                                       >  1:3042146/1‑150 (MQ=255)

                gAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAAt                                                                                                                                      <  2:1066140/150‑1 (MQ=255)

                 aGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATa                                                                                                                                     <  2:3518635/150‑1 (MQ=255)

                   tttATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATaaa                                                                                                                                   <  1:3815479/150‑1 (MQ=255)

                     tATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAgc                                                                                                                                 >  1:2764196/1‑150 (MQ=255)

                      aTCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAgcg                                                                                                                                <  1:2624275/150‑1 (MQ=255)

                              gACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTaaa                                                                                                                        >  1:431706/1‑150 (MQ=255)

                              gACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTaaa                                                                                                                        >  2:2801044/1‑150 (MQ=255)

                               aCCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTaaaa                                                                                                                       >  2:1017500/1‑150 (MQ=255)

                               aCCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTaaaa                                                                                                                       <  2:4164426/150‑1 (MQ=255)

                                ccTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTACCGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAg                                                                                                                      >  2:198487/1‑150 (MQ=255)

                                ccTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAg                                                                                                                      >  1:4419901/1‑150 (MQ=255)

                                 atataTTCTGGAACGTGACTAAGAGATAGTGACGATGATCGTTAACAAGCCTAAGATCATTATCGACTGTAACGAGAAAATGTTTACCGCCTGGTTCTCTGACTTAACGCCAGATACGCTGGTTACTCTCAATAAAGCGCAGCTATAAGc                                                                                                                     <  2:2422227/149‑1 (MQ=255)

                                      ttCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCt                                                                                                                <  1:3277806/150‑1 (MQ=255)

                                      ttCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCt                                                                                                                <  1:3530607/150‑1 (MQ=255)

                                         tGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTggg                                                                                                             <  1:1010836/150‑1 (MQ=255)

                                           gAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGgag                                                                                                           <  2:872779/150‑1 (MQ=255)

                                            aaCGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGgaga                                                                                                          <  2:1771547/150‑1 (MQ=255)

                                            aaCGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGgaga                                                                                                          <  2:2535631/150‑1 (MQ=255)

                                                 gCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAacac                                                                                                     >  2:3317658/1‑150 (MQ=255)

                                                 gCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAacac                                                                                                     <  2:1642592/150‑1 (MQ=255)

                                                    gAAGTGAAAGGGACTCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAAATGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAATAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGc                                                                                                  <  1:114074/150‑1 (MQ=255)

                                                     aaGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCg                                                                                                 <  2:4075815/150‑1 (MQ=255)

                                                      agagAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGa                                                                                                >  2:1716676/1‑150 (MQ=255)

                                                      agagAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGa                                                                                                <  1:805992/150‑1 (MQ=255)

                                                         gaAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAcat                                                                                             <  1:241554/150‑1 (MQ=255)

                                                         gaAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAcat                                                                                             >  1:2373690/1‑150 (MQ=255)

                                                          aaaGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAcatc                                                                                            <  2:2281861/150‑1 (MQ=255)

                                                          aaaGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAcatc                                                                                            >  2:2517682/1‑150 (MQ=255)

                                                            aGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGAcatcat                                                                                          >  2:3336297/1‑150 (MQ=255)

                                                             gggACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCAtt                                                                                         <  1:1022345/150‑1 (MQ=255)

                                                              ggACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTc                                                                                        <  1:1091520/150‑1 (MQ=255)

                                                                aCGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCtt                                                                                      <  1:4019021/150‑1 (MQ=255)

                                                                    tGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGc                                                                                  >  2:2190826/1‑150 (MQ=255)

                                                                    tGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGc                                                                                  >  2:3011382/1‑150 (MQ=255)

                                                                    tGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGc                                                                                  <  1:4246751/150‑1 (MQ=255)

                                                                    tGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGc                                                                                  <  1:2480732/150‑1 (MQ=255)

                                                                      aTCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCg                                                                                <  2:1454435/150‑1 (MQ=255)

                                                                         gTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTg                                                                             <  1:128490/150‑1 (MQ=255)

                                                                         gTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTg                                                                             >  2:346131/1‑150 (MQ=255)

                                                                          ttAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTgg                                                                            <  1:842558/150‑1 (MQ=255)

                                                                           tAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGa                                                                           >  1:2683996/1‑150 (MQ=255)

                                                                            aaCAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGAc                                                                          >  2:3761307/1‑150 (MQ=255)

                                                                            aaCAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGAc                                                                          >  2:1125731/1‑150 (MQ=255)

                                                                            aaCAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGAc                                                                          <  2:3972003/150‑1 (MQ=255)

                                                                             aCAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACg                                                                         <  2:3677219/150‑1 (MQ=255)

                                                                             aCAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACg                                                                         <  1:748958/150‑1 (MQ=255)

                                                                             aCAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACg                                                                         >  2:3125254/1‑150 (MQ=255)

                                                                                   cGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATgg                                                                   <  1:1232728/150‑1 (MQ=255)

                                                                                   cGCAGAGCCTGAGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATgg                                                                   <  1:4509385/150‑1 (MQ=255)

                                                                                        agCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGggcggc                                                              >  1:1432997/1‑150 (MQ=255)

                                                                                         gCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGTGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGgcggcg                                                             <  2:4204097/150‑1 (MQ=255)

                                                                                         gCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGgcggcg                                                             <  1:465598/150‑1 (MQ=255)

                                                                                         gCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGgcggcg                                                             <  1:19963/150‑1 (MQ=255)

                                                                                             gcgcGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTc                                                         >  1:4611720/1‑150 (MQ=255)

                                                                                                 gACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGAtt                                                     >  1:523166/1‑150 (MQ=255)

                                                                                                 gACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGAtt                                                     >  1:2219263/1‑150 (MQ=255)

                                                                                                    tGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTc                                                  >  2:925988/1‑150 (MQ=255)

                                                                                                    tGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTc                                                  >  1:1889730/1‑150 (MQ=255)

                                                                                                    tGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTc                                                  <  1:2213720/150‑1 (MQ=255)

                                                                                                      tAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCcg                                                <  2:3795511/150‑1 (MQ=255)

                                                                                                       aaCGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCcgc                                               >  2:1787330/1‑150 (MQ=255)

                                                                                                               aCTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAaaga                                       <  2:2660289/150‑1 (MQ=255)

                                                                                                                cTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAaagaa                                      <  1:3134483/150‑1 (MQ=255)

                                                                                                                 tGTTTACAGCCTGGTTCTCTGACTTAACGACAGAAACGCTGGTTACGCGCAAGAAAGCGCAGCTTAAAGCGTTCTGTGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAaagaag                                     <  2:3833087/150‑1 (MQ=255)

                                                                                                                    ttACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCg                                  <  2:4565552/150‑1 (MQ=255)

                                                                                                                      aCCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGAt                                <  1:1700779/150‑1 (MQ=255)

                                                                                                                       ccGCCTGGTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATc                               <  1:3660785/150‑1 (MQ=255)

                                                                                                                          ccTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCaa                            <  1:129570/150‑1 (MQ=255)

                                                                                                                          ccTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCaa                            <  1:4213247/150‑1 (MQ=255)

                                                                                                                           cTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCaaa                           >  1:605604/1‑150 (MQ=255)

                                                                                                                             ggTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAAcc                         >  1:574092/1‑150 (MQ=255)

                                                                                                                             ggTTCTCTGACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAAcc                         <  1:2325099/150‑1 (MQ=255)

                                                                                                                              gTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCt                        <  1:3408907/150‑1 (MQ=255)

                                                                                                                               ttCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTc                       <  2:758340/150‑1 (MQ=255)

                                                                                                                                  tctGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGc                    <  1:2563808/150‑1 (MQ=255)

                                                                                                                                  tctGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGc                    <  2:4393384/150‑1 (MQ=255)

                                                                                                                                   ctGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCg                   <  1:3335763/150‑1 (MQ=255)

                                                                                                                                   ctGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCg                   >  2:4149926/1‑150 (MQ=255)

                                                                                                                                    tGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGt                  >  2:1095378/1‑150 (MQ=255)

                                                                                                                                    tGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGt                  >  1:921146/1‑150 (MQ=255)

                                                                                                                                    tGACTTAACGACAGAAAAGCTGTTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCTAACCTCGGCGt                  <  2:137970/150‑1 (MQ=255)

                                                                                                                                     gACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTg                 >  1:112878/1‑150 (MQ=255)

                                                                                                                                     gACTTAACGCCAGAAAAGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTg                 >  1:1826083/1‑150 (MQ=255)

                                                                                                                                       cTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGAt               <  2:3796339/150‑1 (MQ=255)

                                                                                                                                        ttAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGAtt              >  2:3800007/1‑150 (MQ=255)

                                                                                                                                        ttAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGAtt              >  2:1213328/1‑150 (MQ=255)

                                                                                                                                           aCGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGcc           <  2:3960688/150‑1 (MQ=255)

                                                                                                                                                aGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAAc      <  2:3472849/150‑1 (MQ=255)

                                                                                                                                                 gAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTCTGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAAcc     >  1:674254/1‑150 (MQ=255)

                                                                                                                                                  aaaCGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAAccc    >  2:3385948/1‑150 (MQ=255)

                                                                                                                                                    aCGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCctg  <  1:4250158/150‑1 (MQ=255)

CCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTTTACCGCCTGGTTCTCTGACTTAACGCCAGAAACGCTGGTTACGCGCAATAAAGCGCAGCTAAAAGCGTTCTGGGAGAAACACAGCGACATCATTCTTAAGCCGCTGGACGGTATGGGCGGCGCGTCGATTTTCCGCGTGAAAGAAGGCGATCCAAACCTCGGCGTGATTGCCGAAACCCTG  >  CP009273/3085500‑3085797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`