BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,096,445	A→T	21.4%	Q25L (CAA→CTA)	mutY →	adenine DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,096,445	0	A	T	21.4%	602.2 / 174.2	267	Q25L (CAA→CTA)	mutY	adenine DNA glycosylase
Reads supporting (aligned to +/- strand): ref base A (115/95); new base T (34/23); total (149/118)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01

Only 100 of 276 total aligned reads displayed.

CTGCGGAAAGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCC  >  CP009273/3096296‑3096594

cTGCGGAAAGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCa                                                                                                                                                       <  2:4315760/150‑1 (MQ=255)

 tGCGGAAAGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCaa                                                                                                                                                      <  2:3291024/150‑1 (MQ=255)

     gAAAGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTg                                                                                                                                                  <  1:2468505/150‑1 (MQ=255)

       aaGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGAc                                                                                                                                                <  2:4633763/150‑1 (MQ=255)

          ttCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAg                                                                                                                                             >  1:1052242/1‑150 (MQ=255)

          ttCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAg                                                                                                                                             >  2:4230206/1‑150 (MQ=255)

          ttCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAg                                                                                                                                             <  1:1145907/150‑1 (MQ=255)

             cGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAAAAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACg                                                                                                                                          >  1:730425/1‑150 (MQ=255)

              ggTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGc                                                                                                                                         <  2:1528697/150‑1 (MQ=255)

                tttACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGccc                                                                                                                                       <  1:3130404/150‑1 (MQ=255)

                 ttACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCt                                                                                                                                      >  1:507624/1‑150 (MQ=255)

                  tACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTa                                                                                                                                     >  2:3962996/1‑150 (MQ=255)

                   acacCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTAc                                                                                                                                    <  2:299971/150‑1 (MQ=255)

                       ccTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAg                                                                                                                                >  1:641017/1‑150 (MQ=255)

                          gCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTAt                                                                                                                             <  1:1363086/150‑1 (MQ=255)

                          gCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTAt                                                                                                                             <  1:1843763/150‑1 (MQ=255)

                             gTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGc                                                                                                                          >  1:2958711/1‑150 (MQ=255)

                               cGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGctc                                                                                                                        >  2:4562364/1‑150 (MQ=255)

                                gCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGctct                                                                                                                       <  2:2571863/150‑1 (MQ=255)

                                    tgctgcAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGa                                                                                                                   >  1:909537/1‑150 (MQ=255)

                                    tgctgcAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGa                                                                                                                   >  2:2833172/1‑150 (MQ=255)

                                      ctgcAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAg                                                                                                                 <  2:1911882/150‑1 (MQ=255)

                                       tgcAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGt                                                                                                                >  2:1888948/1‑150 (MQ=255)

                                       tgcAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGt                                                                                                                >  2:2276153/1‑150 (MQ=255)

                                         cAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGa                                                                                                              >  1:3653482/1‑150 (MQ=255)

                                              ttGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTg                                                                                                         >  2:2742332/1‑150 (MQ=255)

                                              ttGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTg                                                                                                         >  2:96345/1‑150 (MQ=255)

                                                 cccccAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGcaa                                                                                                      >  2:363873/1‑150 (MQ=255)

                                                  ccccAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGcaac                                                                                                     >  2:4060974/1‑150 (MQ=255)

                                                  ccccAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGcaac                                                                                                     <  2:1078306/150‑1 (MQ=255)

                                                  ccccAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGcaac                                                                                                     <  1:2926115/150‑1 (MQ=255)

                                                  ccccAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGcaac                                                                                                     <  2:363668/150‑1 (MQ=255)

                                                      aacaacAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAAc                                                                                                 >  1:2135895/1‑150 (MQ=255)

                                                         aacaGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCa                                                                                              >  2:1375360/1‑150 (MQ=255)

                                                          acaGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAg                                                                                             >  1:2055850/1‑150 (MQ=255)

                                                           caGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAgg                                                                                            <  2:2266850/150‑1 (MQ=255)

                                                            aGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGt                                                                                           <  1:3719305/150‑1 (MQ=255)

                                                              tGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTg                                                                                         <  1:1441436/150‑1 (MQ=255)

                                                              tGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTg                                                                                         >  2:1483508/1‑150 (MQ=255)

                                                               gAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGc                                                                                        <  1:3006787/150‑1 (MQ=255)

                                                                 aTTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGa                                                                                      <  1:1421664/150‑1 (MQ=255)

                                                                 aTTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGa                                                                                      <  1:2408741/150‑1 (MQ=255)

                                                                   tCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGAcc                                                                                    <  2:2462035/150‑1 (MQ=255)

                                                                   tCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGAcc                                                                                    >  1:1782116/1‑150 (MQ=255)

                                                                     ggTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGt                                                                                  <  2:3984995/150‑1 (MQ=255)

                                                                      gTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGtt                                                                                 >  1:2394291/1‑150 (MQ=255)

                                                                      gTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGtt                                                                                 <  1:2928702/150‑1 (MQ=255)

                                                                        gACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTAt                                                                               >  1:2712236/1‑150 (MQ=255)

                                                                        gACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTAt                                                                               <  2:880569/150‑1 (MQ=255)

                                                                        gACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTAt                                                                               <  2:1828503/150‑1 (MQ=255)

                                                                         aCCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTCCAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATc                                                                              >  2:3891857/1‑150 (MQ=255)

                                                                             tGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCt                                                                          <  2:587272/150‑1 (MQ=255)

                                                                             tGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCt                                                                          >  2:2910649/1‑150 (MQ=255)

                                                                              gCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTa                                                                         <  1:1633999/150‑1 (MQ=255)

                                                                              gCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTa                                                                         <  1:4067568/150‑1 (MQ=255)

                                                                                aaGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTAtt                                                                       <  1:879341/150‑1 (MQ=255)

                                                                                 aGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTAttt                                                                      <  2:392827/150‑1 (MQ=255)

                                                                                 aGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTAttt                                                                      >  1:1779099/1‑150 (MQ=255)

                                                                                  gCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTAtttt                                                                     <  1:417593/150‑1 (MQ=255)

                                                                                  gCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTAtttt                                                                     <  2:662157/150‑1 (MQ=255)

                                                                                      cGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAAc                                                                 >  2:4453500/1‑150 (MQ=255)

                                                                                        cAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAATTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCACATATATTGAACGc                                                               >  1:3247571/1‑150 (MQ=255)

                                                                                           ttttCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCttt                                                            >  2:4310765/1‑150 (MQ=255)

                                                                                            tctCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTa                                                           >  2:2087111/3‑150 (MQ=255)

                                                                                               cAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATgg                                                        >  1:2569371/1‑150 (MQ=255)

                                                                                                aGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGgc                                                       <  1:4412473/150‑1 (MQ=255)

                                                                                                aGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGgc                                                       >  2:2700086/1‑150 (MQ=255)

                                                                                                aGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGgc                                                       >  2:1925536/1‑150 (MQ=255)

                                                                                                   ccAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGgcgcg                                                    >  2:1715452/1‑150 (MQ=255)

                                                                                                    cAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGgcgcgc                                                   >  2:2812296/1‑150 (MQ=255)

                                                                                                     aGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCt                                                  >  2:3597710/1‑150 (MQ=255)

                                                                                                       gTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTc                                                >  1:3705291/1‑150 (MQ=255)

                                                                                                          cTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCg                                             >  1:2536850/1‑150 (MQ=255)

                                                                                                           tGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGa                                            <  1:1033973/150‑1 (MQ=255)

                                                                                                               cTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGt                                        <  1:160828/150‑1 (MQ=255)

                                                                                                                   tACGATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGTCGCGCTTCCCGACGGTGAcc                                    >  1:4554751/1‑150 (MQ=255)

                                                                                                                   tACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGAcc                                    <  2:1228997/150‑1 (MQ=255)

                                                                                                                    aCGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGCTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCg                                   >  2:4267086/1‑150 (MQ=255)

                                                                                                                      gATAAATACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGAt                                 >  1:3291360/1‑150 (MQ=255)

                                                                                                                      gATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGAt                                 >  2:721192/1‑150 (MQ=255)

                                                                                                                       aTAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGAtc                                >  1:532518/1‑150 (MQ=255)

                                                                                                                         aaaTACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGAtctc                              >  2:139797/1‑150 (MQ=255)

                                                                                                                          aaTACGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCg                             >  2:335693/1‑150 (MQ=255)

                                                                                                                           aTACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGc                            <  1:2232152/150‑1 (MQ=255)

                                                                                                                             aCGGGCGAAAAACTCTGCCCTGGCTAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCa                          >  1:2939446/1‑150 (MQ=255)

                                                                                                                             aCGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCa                          >  2:23396/1‑150 (MQ=255)

                                                                                                                              cGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCaa                         >  2:4603395/1‑150 (MQ=255)

                                                                                                                               gggCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAAt                        <  2:383437/150‑1 (MQ=255)

                                                                                                                               gggCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAAt                        >  1:3217891/1‑150 (MQ=255)

                                                                                                                                 gCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATgc                      <  1:2833172/150‑1 (MQ=255)

                                                                                                                                  cGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATgcg                     <  1:972376/150‑1 (MQ=255)

                                                                                                                                   gAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCGTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATgcgc                    >  2:744534/1‑150 (MQ=255)

                                                                                                                                       aaCTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCt                >  2:3673717/1‑150 (MQ=255)

                                                                                                                                       aaCTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCt                >  1:3799426/1‑150 (MQ=255)

                                                                                                                                        aCTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTc               >  1:2427159/1‑150 (MQ=255)

                                                                                                                                         ctctGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTcg              <  2:730047/150‑1 (MQ=255)

                                                                                                                                          tctGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTcga             >  2:4026227/1‑150 (MQ=255)

                                                                                                                                                 tGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGtt      >  1:4267183/1‑150 (MQ=255)

                                                                                                                                                    cAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTtctc   >  2:1622209/1‑150 (MQ=255)

                                                                                                                                                     aaaTTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTcc  <  1:2812249/150‑1 (MQ=255)

CTGCGGAAAGTTCCGGTTTACACCCTGCCGTCGCTGTGCTGCAATCTTGCCCCCAACAACAGTGAATTCGGTGACCATGCAAGCGTCGCAATTTTCAGCCCAGGTTCTGGACTGGTACGATAAATACGGGCGAAAAACTCTGCCCTGGCAAATTGACAAGACGCCCTACAAAGTATGGCTCTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTTATCCCCTATTTTGAACGCTTTATGGCGCGCTTCCCGACGGTGACCGATCTCGCCAATGCGCCGCTCGACGAAGTTCTCC  >  CP009273/3096296‑3096594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`