Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,100,053 C→T 16.2% I327I (ATC→ATT)  nupG → nucleoside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,100,0530CT16.2% 821.1 / 121.2 297I327I (ATC→ATT) nupGnucleoside transporter
Reads supporting (aligned to +/- strand):  ref base C (121/127);  new base T (25/23);  total (147/150)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 305 total aligned reads displayed.

TACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCAT  >  CP009273/3099904‑3100202
                                                                                                                                                     |                                                                                                                                                     
  cGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTAAGGTTGCGCATTCGACTTCTTCAACAtctc                                                                                                                                                     >  1:185442/1‑150 (MQ=255)
    gTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTg                                                                                                                                                   >  2:4451056/1‑150 (MQ=255)
       ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGtt                                                                                                                                                <  1:4592952/150‑1 (MQ=255)
        tAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTc                                                                                                                                               <  2:2551658/150‑1 (MQ=255)
          aGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCgg                                                                                                                                             <  2:2411413/150‑1 (MQ=255)
           gAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGgt                                                                                                                                            <  1:2721185/150‑1 (MQ=255)
           gAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGgt                                                                                                                                            <  2:2990942/150‑1 (MQ=255)
            aaCGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGgtg                                                                                                                                           >  1:3905770/1‑150 (MQ=255)
            aaCGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGgtg                                                                                                                                           >  2:2618081/1‑150 (MQ=255)
                tAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTg                                                                                                                                       <  2:1195230/150‑1 (MQ=255)
                  atgatgATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTc                                                                                                                                     >  1:4307756/1‑150 (MQ=255)
                    gatgatCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGa                                                                                                                                   <  2:4173649/150‑1 (MQ=255)
                    gatgatCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGa                                                                                                                                   >  2:30642/1‑150 (MQ=255)
                     atgatCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGaa                                                                                                                                  <  2:1288351/150‑1 (MQ=255)
                       gatCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGaaaa                                                                                                                                <  1:1208033/150‑1 (MQ=255)
                         tCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAaag                                                                                                                              >  2:2280284/1‑150 (MQ=255)
                          cAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAaaga                                                                                                                             <  2:216413/150‑1 (MQ=255)
                           aGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAaagaa                                                                                                                            <  1:3337976/150‑1 (MQ=255)
                           aGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAaagaa                                                                                                                            <  1:1014788/150‑1 (MQ=255)
                           aGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAaagaa                                                                                                                            <  1:2315246/150‑1 (MQ=255)
                            gTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAaagaag                                                                                                                           <  1:3996261/150‑1 (MQ=255)
                            gTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAaagaag                                                                                                                           >  1:962252/1‑150 (MQ=255)
                               ttGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTa                                                                                                                        <  1:2624919/150‑1 (MQ=255)
                               ttGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTa                                                                                                                        >  1:560609/1‑150 (MQ=255)
                                tgtgGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAg                                                                                                                       <  2:1492848/150‑1 (MQ=255)
                                tgtgGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAg                                                                                                                       >  2:2007158/1‑150 (MQ=255)
                                   ggCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGccc                                                                                                                    >  2:3782666/1‑150 (MQ=255)
                                    gCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCg                                                                                                                   <  2:329077/150‑1 (MQ=255)
                                       tGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCa                                                                                                                <  1:3184804/150‑1 (MQ=255)
                                       tGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCa                                                                                                                <  1:3352714/150‑1 (MQ=255)
                                       tGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCa                                                                                                                >  1:5760/1‑150 (MQ=255)
                                       tGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCa                                                                                                                >  1:4567402/1‑150 (MQ=255)
                                          aTCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAAtt                                                                                                             >  2:3681147/1‑150 (MQ=255)
                                          aTCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAAtt                                                                                                             <  1:3676010/150‑1 (MQ=255)
                                            ccTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTcg                                                                                                           <  2:3497945/150‑1 (MQ=255)
                                            ccTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTcg                                                                                                           >  2:3084857/1‑150 (MQ=255)
                                             cTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTcgc                                                                                                          <  1:553426/150‑1 (MQ=255)
                                             cTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTcgc                                                                                                          <  1:2097310/150‑1 (MQ=255)
                                                cGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGcc                                                                                                       >  1:255359/1‑150 (MQ=255)
                                                 gTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTAGAAAAAGAAGTTAGCCCGGCAATTCGCGCCa                                                                                                      >  2:1333811/1‑150 (MQ=255)
                                                 gTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGAATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCa                                                                                                      >  2:916781/1‑150 (MQ=255)
                                                  ttttGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAg                                                                                                     >  2:545166/1‑150 (MQ=255)
                                                   tttGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTATCCCGGCAATTCGCGCCAGt                                                                                                    <  2:765871/150‑1 (MQ=255)
                                                    ttGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTg                                                                                                   <  2:3421505/150‑1 (MQ=255)
                                                        gcTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGcaca                                                                                               >  1:3006485/1‑150 (MQ=255)
                                                        gcTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGcaca                                                                                               <  2:1714009/150‑1 (MQ=255)
                                                         cTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACaa                                                                                              >  1:1385731/1‑150 (MQ=255)
                                                          tGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAg                                                                                             >  2:4006955/1‑150 (MQ=255)
                                                          tGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAg                                                                                             >  1:3456982/1‑150 (MQ=255)
                                                          tGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAg                                                                                             <  2:3253423/150‑1 (MQ=255)
                                                           gTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAgg                                                                                            >  1:1083700/1‑150 (MQ=255)
                                                            tttGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAggg                                                                                           <  2:3384110/150‑1 (MQ=255)
                                                                     ggCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCtg                                                                                  >  1:3652788/1‑150 (MQ=255)
                                                                     ggCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCtg                                                                                  >  2:2121005/1‑150 (MQ=255)
                                                                     ggCCACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCACAGGTTCGGTGTTTGTAGAAAAAGAAGTTAGCCCGGCAATTAGCGCCAGTGCACAAGGGATGTTCCtg                                                                                  >  2:844707/1‑150 (MQ=255)
                                                                      gCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCtga                                                                                 <  1:703861/150‑1 (MQ=255)
                                                                        gACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCtgatg                                                                               >  2:380669/1‑150 (MQ=255)
                                                                        gACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCtgatg                                                                               >  1:432757/1‑150 (MQ=255)
                                                                        gACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCtgatg                                                                               <  1:1579996/150‑1 (MQ=255)
                                                                          cccGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCtgatgat                                                                             <  2:580366/150‑1 (MQ=255)
                                                                            cGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCtgatgatga                                                                           <  1:31620/150‑1 (MQ=255)
                                                                             gACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGAc                                                                          >  1:2904774/1‑150 (MQ=255)
                                                                              aCTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACt                                                                         <  2:628863/150‑1 (MQ=255)
                                                                               cTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTa                                                                        >  1:2308473/1‑150 (MQ=255)
                                                                                tCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTaa                                                                       >  2:4295866/1‑150 (MQ=255)
                                                                                  cGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACg                                                                     <  1:3489221/150‑1 (MQ=255)
                                                                                  cGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACg                                                                     <  1:1687624/150‑1 (MQ=255)
                                                                                      cGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCtt                                                                 >  1:3090079/1‑150 (MQ=255)
                                                                                       ggTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTc                                                                >  1:1499206/1‑150 (MQ=255)
                                                                                       ggTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTc                                                                >  1:4254580/1‑150 (MQ=255)
                                                                                          actgtactgCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGc                                                             >  2:2227336/1‑150 (MQ=255)
                                                                                          actgtactgCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGc                                                             >  2:2894225/1‑150 (MQ=255)
                                                                                             gtactgCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGt                                                          <  1:1470418/150‑1 (MQ=255)
                                                                                                  gctgGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCt                                                     <  1:1324590/150‑1 (MQ=255)
                                                                                                   ctgGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATTTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAAATCGCGCCAGTGCACCAGGGATGTTCCAGATGATGACTAACGGCTTCGGCTGTATCCTc                                                    >  1:3597215/1‑150 (MQ=255)
                                                                                                   ctgGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCTATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTc                                                    <  1:4640949/150‑1 (MQ=255)
                                                                                                            tCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATc                                           <  1:1774026/150‑1 (MQ=255)
                                                                                                                   tCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCg                                    >  1:2475248/1‑150 (MQ=255)
                                                                                                                   tCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCg                                    >  1:3254416/1‑150 (MQ=255)
                                                                                                                      tttACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTa                                 >  2:3859008/1‑150 (MQ=255)
                                                                                                                          cGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAgt                             >  1:4067300/1‑150 (MQ=255)
                                                                                                                           ggTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAgtt                            <  1:3470929/150‑1 (MQ=255)
                                                                                                                                cgcATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTga                       <  2:4551061/150‑1 (MQ=255)
                                                                                                                                  cATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTgaga                     >  1:552831/1‑150 (MQ=255)
                                                                                                                                   aTTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGAt                    >  1:4228842/1‑150 (MQ=255)
                                                                                                                                   aTTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGAt                    <  2:1919542/150‑1 (MQ=255)
                                                                                                                                       gACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTac                >  2:3039311/1‑150 (MQ=255)
                                                                                                                                        aCTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTTTCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTaca               <  1:3408810/150‑1 (MQ=255)
                                                                                                                                        aCTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTaca               >  1:1649665/1‑150 (MQ=255)
                                                                                                                                         cttcttCAACATTTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTacac              >  1:1932938/1‑150 (MQ=255)
                                                                                                                                          ttcttcAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGGTGTACAcc             >  2:1822927/1‑150 (MQ=255)
                                                                                                                                           tcttcAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACAccc            <  2:2021471/150‑1 (MQ=255)
                                                                                                                                                aaCATCTCTGGTTCGGTGTTTGTCGAAAAAGATGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAAc       <  1:2373190/150‑1 (MQ=255)
                                                                                                                                                aaCATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCACAAc       >  1:828273/1‑150 (MQ=255)
                                                                                                                                                aaCATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAAc       <  1:1244530/150‑1 (MQ=255)
                                                                                                                                                 aCATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACg      <  1:2007169/150‑1 (MQ=255)
                                                                                                                                                 aCATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACg      >  1:3718626/1‑150 (MQ=255)
                                                                                                                                                  cATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACgg     >  2:2347238/1‑150 (MQ=255)
                                                                                                                                                  cATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACgg     >  2:2714841/1‑150 (MQ=255)
                                                                                                                                                  cATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACgg     <  1:3051373/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGGTACTGTCGATGATCGTTTACGGTTGCGCATTCGACTTCTTCAACATCTCTGGTTCGGTGTTTGTCGAAAAAGAAGTTAGCCCGGCAATTCGCGCCAGTGCACAAGGGATGTTCCTGATGATGACTAACGGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCAT  >  CP009273/3099904‑3100202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑