Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,129,198 C→A 32.6% R178L (CGA→CTA)  pitB ← phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,129,1980CA32.6% 519.2 / 244.7 276R178L (CGA→CTA) pitBphosphate transporter
Reads supporting (aligned to +/- strand):  ref base C (98/88);  new base A (54/36);  total (152/124)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 280 total aligned reads displayed.

TCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGA  >  CP009273/3129049‑3129345
                                                                                                                                                     |                                                                                                                                                   
      cGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCAGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCgcagca                                                                                                                                               >  2:4616440/1‑150 (MQ=255)
                   cacgccCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGcc                                                                                                                                  >  1:2294805/1‑150 (MQ=255)
                    acgccCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCt                                                                                                                                 <  2:80397/150‑1 (MQ=255)
                         cGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGc                                                                                                                            <  1:28311/150‑1 (MQ=255)
                            aGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAAt                                                                                                                         >  2:2803888/1‑150 (MQ=255)
                            aGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAAt                                                                                                                         >  2:3701225/1‑150 (MQ=255)
                              cGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGa                                                                                                                       <  2:4388081/150‑1 (MQ=255)
                              cGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGa                                                                                                                       <  2:2869629/150‑1 (MQ=255)
                              cGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGACTATCAGGCCTCCCGCAATGa                                                                                                                       >  1:2348772/1‑150 (MQ=255)
                              cGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGa                                                                                                                       >  1:3293832/1‑150 (MQ=255)
                              cGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGa                                                                                                                       >  2:2152233/1‑150 (MQ=255)
                               ggAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGAc                                                                                                                      >  2:2033904/1‑150 (MQ=255)
                               ggAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTAGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGAc                                                                                                                      >  1:4267698/1‑150 (MQ=255)
                                 aaaCAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCa                                                                                                                    <  1:1529780/150‑1 (MQ=255)
                                  aaCAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAg                                                                                                                   <  1:1365538/150‑1 (MQ=255)
                                  aaCAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAg                                                                                                                   <  2:3534064/150‑1 (MQ=255)
                                   aCAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAgg                                                                                                                  <  2:2949701/150‑1 (MQ=255)
                                        cAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCcgac                                                                                                             <  2:1881137/150‑1 (MQ=255)
                                        cAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCcgac                                                                                                             <  1:1287019/150‑1 (MQ=255)
                                        cAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCcgac                                                                                                             <  2:2474460/150‑1 (MQ=255)
                                         aGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCcgacg                                                                                                            >  2:1983180/1‑150 (MQ=255)
                                            gcAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATa                                                                                                         >  1:203196/1‑150 (MQ=255)
                                            gcAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATa                                                                                                         <  1:3781272/150‑1 (MQ=255)
                                                  cgcgTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGaa                                                                                                   <  1:2626770/150‑1 (MQ=255)
                                                   gcgTCCAGAAGGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGaaa                                                                                                  <  2:1202510/150‑1 (MQ=255)
                                                    cgTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAAc                                                                                                 >  2:2678259/1‑150 (MQ=255)
                                                    cgTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAAc                                                                                                 >  1:1588807/1‑150 (MQ=255)
                                                     gTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACa                                                                                                <  2:1492892/150‑1 (MQ=255)
                                                      tCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACaa                                                                                               >  2:2346840/1‑150 (MQ=255)
                                                       ccAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAAt                                                                                              >  2:2200894/1‑150 (MQ=255)
                                                        cAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATc                                                                                             >  1:4624467/1‑150 (MQ=255)
                                                         aGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCa                                                                                            >  1:4322378/1‑150 (MQ=255)
                                                         aGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCa                                                                                            <  2:106118/150‑1 (MQ=255)
                                                          gAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAg                                                                                           <  2:1948284/150‑1 (MQ=255)
                                                          gAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAg                                                                                           >  2:3183361/1‑150 (MQ=255)
                                                            aTGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCg                                                                                         <  1:4378768/150‑1 (MQ=255)
                                                              ggCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCgag                                                                                       <  1:1863723/150‑1 (MQ=255)
                                                                cGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGAGAGGGGAAACAATCAGCgagga                                                                                     >  2:1536654/1‑150 (MQ=255)
                                                                cGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCgagga                                                                                     >  1:775990/1‑150 (MQ=255)
                                                                    ttACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGaaa                                                                                 <  2:1139077/150‑1 (MQ=255)
                                                                     tACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGaaaa                                                                                >  2:3336823/1‑150 (MQ=255)
                                                                      aCGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAAt                                                                               >  2:1743642/1‑150 (MQ=255)
                                                                         tttGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAAtttt                                                                            >  1:9329/1‑150 (MQ=255)
                                                                          ttGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTg                                                                           <  1:3441254/150‑1 (MQ=255)
                                                                             ccTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTc                                                                        >  1:4272624/1‑150 (MQ=255)
                                                                             ccTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTc                                                                        >  1:4272585/1‑150 (MQ=255)
                                                                             ccTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTc                                                                        >  1:1466043/1‑150 (MQ=255)
                                                                               ttttttCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCAc                                                                      >  2:3401367/1‑150 (MQ=255)
                                                                                tttttCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACt                                                                     >  2:3488121/1‑150 (MQ=255)
                                                                                  tttCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTc                                                                   >  2:1358022/1‑150 (MQ=255)
                                                                                   ttCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCa                                                                  >  1:3610923/1‑150 (MQ=255)
                                                                                         ttGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAgg                                                            <  2:46719/150‑1 (MQ=255)
                                                                                          tGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGt                                                           <  2:4532674/150‑1 (MQ=255)
                                                                                           gCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGtt                                                          >  1:1911805/1‑150 (MQ=255)
                                                                                            cGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGttt                                                         <  2:3947095/150‑1 (MQ=255)
                                                                                             gATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTa                                                        <  1:3766571/150‑1 (MQ=255)
                                                                                                cTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACg                                                     >  1:3537293/1‑150 (MQ=255)
                                                                                                cTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACg                                                     >  2:628086/1‑150 (MQ=255)
                                                                                                 ttCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGc                                                    >  1:3794856/1‑150 (MQ=255)
                                                                                                   ccGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGcat                                                  >  1:3093937/1‑150 (MQ=255)
                                                                                                    cGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGcatc                                                 >  2:2300854/1‑150 (MQ=255)
                                                                                                    cGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGcatc                                                 >  1:2876922/1‑150 (MQ=255)
                                                                                                     ggAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGcatcc                                                >  2:3021324/1‑150 (MQ=255)
                                                                                                     ggAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGcatcc                                                <  1:4172635/150‑1 (MQ=255)
                                                                                                      gAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGcatcca                                               <  1:2752135/150‑1 (MQ=255)
                                                                                                      gAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGcatcca                                               <  1:3010510/150‑1 (MQ=255)
                                                                                                      gAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGcatcca                                               <  1:690391/150‑1 (MQ=255)
                                                                                                       aaTGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGcatccat                                              >  1:999319/1‑150 (MQ=255)
                                                                                                            ggTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCg                                         >  2:3269050/1‑150 (MQ=255)
                                                                                                            ggTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCg                                         >  2:3199319/1‑150 (MQ=255)
                                                                                                              tGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGAt                                       <  2:3307725/150‑1 (MQ=255)
                                                                                                              tGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGAt                                       <  1:1732282/150‑1 (MQ=255)
                                                                                                               gAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATg                                      >  1:4519210/1‑150 (MQ=255)
                                                                                                                aaTACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGa                                     <  1:556483/150‑1 (MQ=255)
                                                                                                                aaTACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGa                                     <  2:4079889/150‑1 (MQ=255)
                                                                                                                 aTACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAg                                    >  1:2653884/1‑150 (MQ=255)
                                                                                                                  tACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGc                                   >  1:3585570/1‑150 (MQ=255)
                                                                                                                     ggTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCgg                                >  2:1079680/1‑150 (MQ=255)
                                                                                                                     ggTCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCgg                                >  1:2043611/1‑150 (MQ=255)
                                                                                                                       tCACGCTTTTTCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGtt                              <  1:1005545/150‑1 (MQ=255)
                                                                                                                       tCACGATTTTTCGTCCCGCTCCAGTATCGTCTCAGCAGGAATATCAGGCCTACATCAATGACATGGCAGACTATAGGGGAAACAATCAGAGAGTAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATAAACGATGAGCCGGtt                              <  2:1393114/150‑1 (MQ=255)
                                                                                                                        cACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTa                             <  2:2534973/150‑1 (MQ=255)
                                                                                                                        cACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTa                             <  2:1228630/150‑1 (MQ=255)
                                                                                                                          cGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAAc                           >  1:1080594/1‑150 (MQ=255)
                                                                                                                          cGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAAc                           <  2:2052752/150‑1 (MQ=255)
                                                                                                                             tttttCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAgc                        >  2:1657712/1‑150 (MQ=255)
                                                                                                                               tttCGTCCCGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAgcgc                      >  2:983609/1‑150 (MQ=255)
                                                                                                                                  cGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGtt                   >  2:4236622/1‑150 (MQ=255)
                                                                                                                                      ccGCTCCAGTAGCGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGtt               >  1:1157066/1‑150 (MQ=255)
                                                                                                                                       cGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTa              <  2:2890880/150‑1 (MQ=255)
                                                                                                                                       cGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTa              <  2:571668/150‑1 (MQ=255)
                                                                                                                                       cGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTa              >  1:1258371/1‑150 (MQ=255)
                                                                                                                                             aGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGa        >  1:573574/1‑150 (MQ=255)
                                                                                                                                             aGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGa        <  1:390073/150‑1 (MQ=255)
                                                                                                                                             aGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGa        >  1:2492596/1‑150 (MQ=255)
                                                                                                                                             aGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGa        >  1:2311316/1‑150 (MQ=255)
                                                                                                                                               tAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATg      >  2:4322678/1‑150 (MQ=255)
                                                                                                                                                aGCGTCGCTGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGTTTTAACGCATCCATCACCGATGAGCCGGATAACAGCGCGTTGGTTAAACAGATga     >  2:1305932/1‑149 (MQ=255)
                                                                                                                                                  cGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCg   <  1:2993055/150‑1 (MQ=255)
                                                                                                                                                  cGTAGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCg   <  1:2789699/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACGTTTGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTCACGCTTTTTCGTCCCGCTCCAGTAGCGTCGCAGCAGGAATATCAGGCCTCCCGCAATGACCAGGCCGACGATAGGGGAAACAATCAGCGAGGAGAAAATTTTGGTCACTTCACGCAGGTTTAACGCATCCATCACCGATGAGCCGGTTAACAGCGCGTTGGTTAAACCGATGCCGA  >  CP009273/3129049‑3129345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑