Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,169,865 T→G 10.2% I110L (ATC→CTC)  cpdA ← 3',5' cAMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,169,8650TG10.2% 731.6 / 85.6 275I110L (ATC→CTC) cpdA3',5' cAMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (117/130);  new base G (14/14);  total (131/144)
Fisher's exact test for biased strand distribution p-value = 8.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

Only 100 of 279 total aligned reads displayed.

CGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCG  >  CP009273/3169716‑3170013
                                                                                                                                                     |                                                                                                                                                    
  tATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATAc                                                                                                                                                    >  1:1004376/1‑150 (MQ=255)
  tATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATAc                                                                                                                                                    >  2:554781/1‑150 (MQ=255)
   aTGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGAGAcc                                                                                                                                                   >  2:3569792/1‑150 (MQ=255)
    tGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATAccc                                                                                                                                                  <  2:1076181/150‑1 (MQ=255)
    tGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATAccc                                                                                                                                                  <  1:810380/150‑1 (MQ=255)
       cGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCa                                                                                                                                               >  2:1828435/1‑150 (MQ=255)
        gTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGAGACCCGCAt                                                                                                                                              >  2:149388/1‑150 (MQ=255)
          tCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATcc                                                                                                                                            >  1:4543985/1‑150 (MQ=255)
                 cATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAAcg                                                                                                                                     >  2:4476587/1‑150 (MQ=255)
                 cATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAAcg                                                                                                                                     >  1:4499231/1‑150 (MQ=255)
                  aTCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAAcgc                                                                                                                                    >  1:945415/1‑150 (MQ=255)
                  aTCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAAcgc                                                                                                                                    <  1:4566900/150‑1 (MQ=255)
                     ggCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCt                                                                                                                                 <  2:600623/150‑1 (MQ=255)
                       ccAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGt                                                                                                                               >  1:2447014/1‑150 (MQ=255)
                        cAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTa                                                                                                                              >  2:4187738/1‑150 (MQ=255)
                               cGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATcgcg                                                                                                                       >  2:1426170/1‑150 (MQ=255)
                                 ttCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCggg                                                                                                                     >  2:467147/1‑150 (MQ=255)
                                 ttCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGAGACCCGCATCCTGTAACGCGCTGTACATCGCggg                                                                                                                     <  1:2301987/150‑1 (MQ=255)
                                   ccAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCt                                                                                                                   <  1:3344642/150‑1 (MQ=255)
                                       ccACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGaa                                                                                                               >  2:4246925/1‑150 (MQ=255)
                                       ccACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGaa                                                                                                               >  2:2383385/1‑150 (MQ=255)
                                        cACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGaaa                                                                                                              >  1:1470780/1‑150 (MQ=255)
                                        cACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGAGACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGaaa                                                                                                              >  2:223458/1‑150 (MQ=255)
                                          cTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATc                                                                                                            <  1:12059/150‑1 (MQ=255)
                                             aaGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTg                                                                                                         <  1:1921229/150‑1 (MQ=255)
                                              aGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTgg                                                                                                        <  1:2539161/150‑1 (MQ=255)
                                              aGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTgg                                                                                                        >  1:1476019/1‑150 (MQ=255)
                                                cTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACCCGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGtt                                                                                                      <  2:2980099/150‑1 (MQ=255)
                                                cTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGtt                                                                                                      <  2:2870514/150‑1 (MQ=255)
                                                   aaaCTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGcc                                                                                                   <  1:3194628/150‑1 (MQ=255)
                                                     aCTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGTATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGTAATCGTGGTTGCCCg                                                                                                 <  1:2734499/150‑1 (MQ=255)
                                                     aCTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCg                                                                                                 <  1:453932/150‑1 (MQ=255)
                                                      cTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCgg                                                                                                >  1:1918160/1‑150 (MQ=255)
                                                        cGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCa                                                                                              <  2:715725/150‑1 (MQ=255)
                                                         gctcagctcaCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAg                                                                                             >  1:755402/1‑150 (MQ=255)
                                                           tcagctcaCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGAGACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGcc                                                                                           <  1:296478/150‑1 (MQ=255)
                                                            cagctcaCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCa                                                                                          <  1:162635/150‑1 (MQ=255)
                                                             agctcaCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAg                                                                                         >  1:697669/1‑150 (MQ=255)
                                                                tcaCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACg                                                                                      <  2:1707067/150‑1 (MQ=255)
                                                                    cGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAgg                                                                                  <  2:664623/150‑1 (MQ=255)
                                                                     gTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAggg                                                                                 >  1:1161093/1‑150 (MQ=255)
                                                                      tGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGgc                                                                                <  2:3508244/150‑1 (MQ=255)
                                                                       gCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGAGACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGgcg                                                                               <  2:2006748/150‑1 (MQ=255)
                                                                         ggCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGAGACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCa                                                                             <  1:2138891/150‑1 (MQ=255)
                                                                           cACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACg                                                                           >  2:3358147/1‑150 (MQ=255)
                                                                           cACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACg                                                                           >  2:4073133/1‑150 (MQ=255)
                                                                           cACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACg                                                                           <  2:1284897/150‑1 (MQ=255)
                                                                              gCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGaaa                                                                        >  1:1516728/1‑150 (MQ=255)
                                                                               ccAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGaaaa                                                                       >  2:1234027/1‑150 (MQ=255)
                                                                                   acacCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTg                                                                   <  1:3504737/150‑1 (MQ=255)
                                                                                    cacCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGc                                                                  >  2:2661661/1‑150 (MQ=255)
                                                                                    cacCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGc                                                                  >  2:816696/1‑150 (MQ=255)
                                                                                       cTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGAt                                                               >  1:1785006/1‑150 (MQ=255)
                                                                                       cTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGAt                                                               <  2:931822/150‑1 (MQ=255)
                                                                                       cTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGAt                                                               <  2:1475971/150‑1 (MQ=255)
                                                                                        tGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATg                                                              <  1:4291749/150‑1 (MQ=255)
                                                                                          gCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACCCGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGcc                                                            <  2:4554115/150‑1 (MQ=255)
                                                                                           cTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATAGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCt                                                           >  1:1002998/1‑150 (MQ=255)
                                                                                            tATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATTCCCGAATCCTGTAACGCGCTGTACATCGCGTGCTGGAAATCGTGGTTGCCCGGCAGACAGACGCAGGGCGCACGAAAACTTGCGATGCCtt                                                          <  2:1139329/150‑1 (MQ=255)
                                                                                            tATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCtt                                                          <  2:3164379/150‑1 (MQ=255)
                                                                                            tATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCtt                                                          <  2:3221487/150‑1 (MQ=255)
                                                                                               ccAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAg                                                       <  1:2899751/150‑1 (MQ=255)
                                                                                               ccAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAg                                                       <  2:1739856/150‑1 (MQ=255)
                                                                                               ccAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAg                                                       <  1:2899276/150‑1 (MQ=255)
                                                                                                 aGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCg                                                     <  1:3013481/150‑1 (MQ=255)
                                                                                                  gCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGa                                                    >  2:2117607/1‑150 (MQ=255)
                                                                                                  gCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGa                                                    <  1:2033089/150‑1 (MQ=255)
                                                                                                   cAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGaa                                                   >  1:2066735/1‑150 (MQ=255)
                                                                                                   cAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGaa                                                   >  1:4396668/1‑150 (MQ=255)
                                                                                                      cAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATg                                                <  1:3931496/150‑1 (MQ=255)
                                                                                                      cAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATg                                                >  2:3419951/1‑150 (MQ=255)
                                                                                                         gATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTg                                             <  1:1372246/150‑1 (MQ=255)
                                                                                                           tttGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGAt                                           <  1:554781/150‑1 (MQ=255)
                                                                                                           tttGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGAGACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGAt                                           <  2:3755072/150‑1 (MQ=255)
                                                                                                            ttGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATa                                          >  1:3840885/1‑150 (MQ=255)
                                                                                                             tGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAg                                         >  1:3262516/1‑150 (MQ=255)
                                                                                                              gCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAgg                                        <  2:4004566/150‑1 (MQ=255)
                                                                                                                cATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGcc                                      >  1:1272338/1‑150 (MQ=255)
                                                                                                                 aTTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCg                                     <  2:315491/150‑1 (MQ=255)
                                                                                                                 aTTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCg                                     <  2:3166311/150‑1 (MQ=255)
                                                                                                                 aTTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCg                                     <  1:3974988/150‑1 (MQ=255)
                                                                                                                   tGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCa                                   <  1:2347305/150‑1 (MQ=255)
                                                                                                                   tGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCa                                   <  2:4132903/150‑1 (MQ=255)
                                                                                                                    gCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCag                                  <  2:4486699/150‑1 (MQ=255)
                                                                                                                        aCCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGa                              <  1:3614404/150‑1 (MQ=255)
                                                                                                                            aTAAACACGCGCTTCGCCGGGGAGAGACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGa                          >  1:469846/1‑150 (MQ=255)
                                                                                                                              aaaCACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATc                        >  2:3816332/1‑150 (MQ=255)
                                                                                                                               aaCACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATcc                       <  2:1132097/150‑1 (MQ=255)
                                                                                                                                      gcTTCGCCGGGGAGAGACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTa                >  1:4030698/1‑150 (MQ=255)
                                                                                                                                         tCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAAt             <  2:2524245/150‑1 (MQ=255)
                                                                                                                                         tCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAAt             >  2:1800566/1‑150 (MQ=255)
                                                                                                                                         tCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAAt             <  2:2718153/150‑1 (MQ=255)
                                                                                                                                         tCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAAt             <  1:382463/150‑1 (MQ=255)
                                                                                                                                          cGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATc            >  1:2782117/1‑150 (MQ=255)
                                                                                                                                            ccGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCAc          <  2:2193146/150‑1 (MQ=255)
                                                                                                                                              ggggAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCt        >  2:3923071/1‑150 (MQ=255)
                                                                                                                                               gggAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTg       >  2:223920/1‑150 (MQ=255)
                                                                                                                                               gggAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTg       <  1:1105881/150‑1 (MQ=255)
                                                                                                                                                  agaTACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTcg    <  1:3066533/150‑1 (MQ=255)
                                                                                                                                                   gaTACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTcgc   >  1:939212/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCG  >  CP009273/3169716‑3170013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑