BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,173,537	G→T	5.3%	P145P (CCG→CCT)	ygiB →	DUF1190 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,173,537	0	G	T	5.3%	1016.5 / 21.8	263	P145P (CCG→CCT)	ygiB	DUF1190 family protein
Reads supporting (aligned to +/- strand): ref base G (120/128); new base T (11/3); total (131/132)
Fisher's exact test for biased strand distribution p-value = 5.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 265 total aligned reads displayed.

CAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTA  >  CP009273/3173388‑3173685

cAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCg                                                                                                                                                      <  1:2237382/150‑1 (MQ=255)

cAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCg                                                                                                                                                      <  1:1792911/150‑1 (MQ=255)

   tGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTg                                                                                                                                                   >  1:797492/1‑150 (MQ=255)

   tGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTg                                                                                                                                                   >  2:670137/1‑150 (MQ=255)

    gCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGt                                                                                                                                                  >  2:2882705/1‑150 (MQ=255)

      cagcagGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTtc                                                                                                                                                >  2:4472478/1‑150 (MQ=255)

         cagGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTctcc                                                                                                                                             >  1:466583/1‑150 (MQ=255)

          agGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTctcct                                                                                                                                            <  1:3395744/150‑1 (MQ=255)

           ggCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTctcctc                                                                                                                                           <  1:1147520/150‑1 (MQ=255)

           ggCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTctcctc                                                                                                                                           <  1:3246369/150‑1 (MQ=255)

             cACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGa                                                                                                                                         <  2:2057978/150‑1 (MQ=255)

             cACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGa                                                                                                                                         >  2:3695411/1‑150 (MQ=255)

              aCCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGaa                                                                                                                                        >  1:421406/1‑150 (MQ=255)

               ccAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGaaa                                                                                                                                       <  1:3362021/150‑1 (MQ=255)

                 aGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGaaaaa                                                                                                                                     <  2:1765919/150‑1 (MQ=255)

                 aGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGaaaaa                                                                                                                                     >  1:1448023/1‑150 (MQ=255)

                        gCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACccagcc                                                                                                                              <  1:4183649/150‑1 (MQ=255)

                        gCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACccagcc                                                                                                                              >  2:2429863/1‑150 (MQ=255)

                         cTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACccagcca                                                                                                                             <  1:1028782/150‑1 (MQ=255)

                            gcatggcaCCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTc                                                                                                                          <  1:703285/150‑1 (MQ=255)

                            gcatggcaCCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTc                                                                                                                          <  1:893217/150‑1 (MQ=255)

                              atggcaCCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCg                                                                                                                        <  1:4520543/150‑1 (MQ=255)

                               tggcaCCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCgg                                                                                                                       <  1:4573357/150‑1 (MQ=255)

                                  caCCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCtt                                                                                                                    <  1:4141790/150‑1 (MQ=255)

                                      aGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACgg                                                                                                                >  2:3960458/1‑150 (MQ=255)

                                         aaaCCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCTCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTaa                                                                                                             <  2:4140907/150‑1 (MQ=255)

                                         aaaCCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCTCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTaa                                                                                                             >  1:4036154/1‑150 (MQ=255)

                                            ccAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAata                                                                                                          >  1:3103704/1‑150 (MQ=255)

                                              aGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAAtata                                                                                                        >  2:3551417/1‑150 (MQ=255)

                                                gcgaAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTTATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGACAGTCCGGCTTACGGTATATATAcc                                                                                                      <  2:1457687/150‑1 (MQ=255)

                                                 cgcAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACcg                                                                                                     >  1:1720967/1‑150 (MQ=255)

                                                  gcAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACcga                                                                                                    >  1:2902847/1‑150 (MQ=255)

                                                  gcAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACcga                                                                                                    >  1:2300763/1‑150 (MQ=255)

                                                   cAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACcgac                                                                                                   <  1:3830171/150‑1 (MQ=255)

                                                    aGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATAAACcgacg                                                                                                  >  2:674842/1‑150 (MQ=255)

                                                         cAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACcgacgcgacg                                                                                             >  1:1050774/1‑150 (MQ=255)

                                                            cAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGt                                                                                          >  2:3246640/1‑150 (MQ=255)

                                                            cAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGt                                                                                          >  2:3588722/1‑150 (MQ=255)

                                                             aaTCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTa                                                                                         >  2:922772/1‑150 (MQ=255)

                                                             aaTCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTa                                                                                         >  1:153057/1‑150 (MQ=255)

                                                              aTCCAGGGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACTGTAAATATACCGACGCGACGGGTaa                                                                                        <  1:2376745/150‑1 (MQ=255)

                                                              aTCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCAAGTCCGGCTTACGGTAAATATACCGACTCGACGGGTat                                                                                        <  2:2088031/150‑2 (MQ=255)

                                                                 cAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTaaaaa                                                                                     >  1:2195351/1‑150 (MQ=255)

                                                                   gCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACt                                                                                   >  1:670133/1‑150 (MQ=255)

                                                                     gggAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTAt                                                                                 <  1:2662710/150‑1 (MQ=255)

                                                                     gggAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTAt                                                                                 >  2:1969890/1‑150 (MQ=255)

                                                                      ggAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATg                                                                                >  2:2389107/1‑150 (MQ=255)

                                                                         gTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGgcg                                                                             <  2:2842576/150‑1 (MQ=255)

                                                                          tttCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGgcgc                                                                            >  2:2189149/1‑150 (MQ=255)

                                                                            tCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGcag                                                                          <  2:1367835/150‑1 (MQ=255)

                                                                             cTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGcagc                                                                         >  2:423698/1‑150 (MQ=255)

                                                                                gATGCCGCTGATGGCCTGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGTTATTTGCACAGCAGCCGATGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGcagccca                                                                      <  1:1168904/150‑1 (MQ=255)

                                                                                 aTGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCTCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGcagcccag                                                                     >  1:1319480/1‑150 (MQ=255)

                                                                                 aTGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGcagcccag                                                                     <  2:1519502/150‑1 (MQ=255)

                                                                                    ccGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCccagcca                                                                  >  2:38055/1‑150 (MQ=255)

                                                                                     cGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCccagccag                                                                 <  2:479807/150‑1 (MQ=255)

                                                                                           tGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCa                                                           <  1:1251041/150‑1 (MQ=255)

                                                                                             gCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCAcc                                                         <  1:2937733/150‑1 (MQ=255)

                                                                                             gCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCAcc                                                         <  1:1009897/150‑1 (MQ=255)

                                                                                              ccGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCa                                                        >  1:3183334/1‑150 (MQ=255)

                                                                                                ggTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATg                                                      <  1:2944991/150‑1 (MQ=255)

                                                                                                  ttACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGAc                                                    <  2:3704818/150‑1 (MQ=255)

                                                                                                   tACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGAcc                                                   <  1:1492220/150‑1 (MQ=255)

                                                                                                   tACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGAcc                                                   <  2:1815580/150‑1 (MQ=255)

                                                                                                       tgatgGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTAc                                               <  2:2817016/150‑1 (MQ=255)

                                                                                                       tgatgGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTAc                                               >  1:4229111/1‑150 (MQ=255)

                                                                                                        gatgGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTAcc                                              <  1:1612335/150‑1 (MQ=255)

                                                                                                         atgGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCg                                             >  2:3764237/1‑150 (MQ=255)

                                                                                                          tgGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGTGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGa                                            >  1:4071023/1‑150 (MQ=255)

                                                                                                          tgGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGa                                            >  1:4418236/1‑150 (MQ=255)

                                                                                                           ggggCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGac                                           >  1:3470981/1‑149 (MQ=255)

                                                                                                           ggggCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGaa                                           <  2:2097318/150‑1 (MQ=255)

                                                                                                           ggggCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGaa                                           >  1:2052959/1‑150 (MQ=255)

                                                                                                            gggCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAg                                          >  1:835398/1‑150 (MQ=255)

                                                                                                                 tCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCTGCTTACGGTATATATACCGACGCGACGGGTAAATACTATGGCGAAGCCCTGCAAGGCCGCACCATGACCGTACCGAAGACGGc                                     <  1:4324287/150‑1 (MQ=255)

                                                                                                                   tGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCaa                                   >  1:593993/1‑150 (MQ=255)

                                                                                                                    gATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTATGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAAt                                  >  2:4032977/1‑150 (MQ=255)

                                                                                                                     aTGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATg                                 <  1:497095/150‑1 (MQ=255)

                                                                                                                       gggCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGc                               <  2:4574093/150‑1 (MQ=255)

                                                                                                                       gggCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGc                               <  2:3914713/150‑1 (MQ=255)

                                                                                                                         gcggcggcgCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCAc                             <  2:4418236/150‑1 (MQ=255)

                                                                                                                         gcggcggcgCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCAc                             <  2:4071008/150‑1 (MQ=255)

                                                                                                                          cggcggcgCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCAcc                            <  1:3609661/150‑1 (MQ=255)

                                                                                                                           ggcggcgCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCa                           <  2:1050774/150‑1 (MQ=255)

                                                                                                                             cggcgCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCaaa                         >  1:2476768/1‑150 (MQ=255)

                                                                                                                                 gcgGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCg                     >  1:854535/1‑150 (MQ=255)

                                                                                                                                   gggATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGc                   >  1:1126583/1‑150 (MQ=255)

                                                                                                                                   gggATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGc                   <  2:543049/150‑1 (MQ=255)

                                                                                                                                    ggATTTGCACAGCAGCCTCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGct                  >  2:1736664/1‑149 (MQ=255)

                                                                                                                                    ggATTTGCACAGCAGCCTCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGct                  >  2:1736877/1‑149 (MQ=255)

                                                                                                                                    ggATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGct                  >  1:2553707/1‑149 (MQ=255)

                                                                                                                                    ggATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGct                  >  1:216825/1‑149 (MQ=255)

                                                                                                                                       tttGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTacc               >  2:3364998/1‑150 (MQ=255)

                                                                                                                                             cagcagCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACt         >  1:1694726/1‑150 (MQ=255)

                                                                                                                                                cagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTAcc      <  1:596420/150‑1 (MQ=255)

                                                                                                                                                cagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTAcc      <  1:3558359/150‑1 (MQ=255)

                                                                                                                                                cagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTAcc      >  2:3433063/1‑150 (MQ=255)

                                                                                                                                                   ccGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGtt   <  2:179120/150‑1 (MQ=255)

                                                                                                                                                    cGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTa  <  2:3183334/150‑1 (MQ=255)

                                                                                                                                                    cGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTa  <  1:746895/150‑1 (MQ=255)

CAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTA  >  CP009273/3173388‑3173685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`