| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,217,098 | C→A | 18.4% | I369I (ATC→ATA) | ebgA → | evolved beta‑D‑galactosidase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,217,098 | 0 | C | A | 18.4% | 794.6 / 114.8 | 261 | I369I (ATC→ATA) | ebgA | evolved beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (90/123); new base A (27/21); total (117/144) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 264 total aligned reads displayed.
TCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCA > CP009273/3216950‑3217247 | aaaGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATAAAc > 2:3050714/1‑150 (MQ=255) gTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTcc > 1:985521/1‑150 (MQ=255) tgagcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACTATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGAGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCg < 2:3510631/147‑1 (MQ=255) gcgcGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGt < 2:862495/150‑1 (MQ=255) gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGc < 1:3713152/150‑1 (MQ=255) cgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCg < 2:4353385/150‑1 (MQ=255) gACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGt > 2:448551/1‑150 (MQ=255) aCGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTa > 1:3442939/1‑150 (MQ=255) cGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTAc > 1:2326861/1‑150 (MQ=255) gTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCg > 1:3598897/1‑150 (MQ=255) tGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTc > 1:1500849/1‑150 (MQ=255) gTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCa < 1:259172/150‑1 (MQ=255) tGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTAc < 2:350112/150‑1 (MQ=255) aTCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCg > 1:3399411/1‑150 (MQ=255) cAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGaa < 2:2183693/150‑1 (MQ=255) aaTAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAAc < 2:2741359/150‑1 (MQ=255) aaTAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAAc > 1:3658073/1‑150 (MQ=255) aaTAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAAc > 2:226992/1‑150 (MQ=255) aaCCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGAt < 1:1898519/150‑1 (MQ=255) ggtATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCcgc > 2:3357518/3‑150 (MQ=255) gTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCcgc < 1:1245921/150‑1 (MQ=255) tATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGt > 2:761103/1‑150 (MQ=255) tgtgATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGttt < 1:400147/150‑1 (MQ=255) tgtgATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGttt < 1:1988924/150‑1 (MQ=255) tgtgATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGttt < 1:1988913/150‑1 (MQ=255) tgtgATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGttt > 2:2035733/1‑150 (MQ=255) tgtgATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGttt < 1:639873/150‑1 (MQ=255) tgATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGttttt > 2:2168801/1‑150 (MQ=255) tgATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGttttt < 1:3309440/150‑1 (MQ=255) gATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTa > 1:2607166/1‑150 (MQ=255) aTGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTAc > 1:1575112/1‑150 (MQ=255) aCGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgt > 2:1357675/1‑150 (MQ=255) ggCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgtgt > 1:254402/1‑150 (MQ=255) ggCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgtgt < 1:1929921/150‑1 (MQ=255) ggCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgtgt > 2:4123246/1‑150 (MQ=255) gCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgtgtg < 1:2959418/150‑1 (MQ=255) gCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgtgtg < 1:312984/150‑1 (MQ=255) gTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGat < 1:4235536/150‑1 (MQ=255) tCAACCGTCACGACAACGATCAGCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGTTGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCTCTACCCGAACGATCCGCGTTTTTACGAACTGTGTGata < 1:4211918/150‑1 (MQ=255) cAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGatat < 1:1085058/150‑1 (MQ=255) aaCCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGTACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATc < 2:3133013/150‑1 (MQ=255) gTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACg > 1:1373684/1‑150 (MQ=255) cACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGc > 2:4485259/1‑150 (MQ=255) cGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCt > 1:2367282/1‑150 (MQ=255) aCAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGt < 1:3972331/150‑1 (MQ=255) cAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGtt < 2:4413746/150‑1 (MQ=255) aaCGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGACCGATCCGCGATTTTACGAACTGTGTGATATCTACGGCCTGttt < 2:1221352/150‑1 (MQ=255) aaCGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGttt < 2:1468936/150‑1 (MQ=255) cGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTtgt < 1:4299973/150‑1 (MQ=255) cGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTtgt < 1:2678162/150‑1 (MQ=255) atcatcGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGAGTTTTTACGAACTGTGTGATATCTAAGGCCTGTTTGTGa > 2:2240408/1‑150 (MQ=255) tcGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGc < 1:240376/150‑1 (MQ=255) tcGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGc < 1:4382258/150‑1 (MQ=255) cGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATggag > 2:1400127/1‑148 (MQ=255) gCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCgg > 1:749385/1‑150 (MQ=255) aaaGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGaa > 2:3939561/1‑150 (MQ=255) aaGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGaaa > 1:2165745/1‑150 (MQ=255) ggCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAAcc > 1:3009754/1‑150 (MQ=255) ggCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAAcc > 1:2497739/1‑150 (MQ=255) cgcgCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGAc < 2:3806979/150‑1 (MQ=255) gcgcCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACg < 2:338181/150‑1 (MQ=255) gcCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTc < 2:2108001/150‑1 (MQ=255) ccGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCg > 2:133696/1‑150 (MQ=255) gTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGaa > 1:3231551/1‑150 (MQ=255) ggAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCg < 2:3405360/150‑1 (MQ=255) ggAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCg < 2:3405381/150‑1 (MQ=255) ggAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCg < 1:3325616/150‑1 (MQ=255) aaTGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCa < 1:1426948/150‑1 (MQ=255) aTCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCt < 1:2843723/150‑1 (MQ=255) tCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCtt < 2:972476/150‑1 (MQ=255) gTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCt > 1:611524/1‑150 (MQ=255) gagaAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAAt < 2:1852607/150‑1 (MQ=255) agaAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATg > 2:1417364/1‑150 (MQ=255) agaAAGATCTCCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATg > 2:1108784/1‑150 (MQ=255) aGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCgg > 2:391197/1‑150 (MQ=255) ctcCAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGat > 2:2406925/1‑150 (MQ=255) tcCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGata < 1:448541/150‑1 (MQ=255) ccAGTTGATGAAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGatat > 1:3257213/1‑150 (MQ=255) cAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATAtt > 1:1602359/1‑150 (MQ=255) aGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTa < 1:4328652/150‑1 (MQ=255) tgatgaAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGcc < 1:566441/150‑1 (MQ=255) gatgaAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGCTCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTTCTGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCg < 1:4509888/150‑1 (MQ=255) gatgaAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCg > 1:881688/1‑150 (MQ=255) atgaAGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGt > 1:2101440/1‑150 (MQ=255) aaGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTAtt < 2:2581084/150‑1 (MQ=255) aaGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTAtt < 1:1760326/150‑1 (MQ=255) aaGCAGCACAATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTAtt > 2:4413438/1‑150 (MQ=255) cagcaCAATATCAACTCCGTGCGTACCGCTCACTTCCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAATCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAcc < 2:2832567/150‑1 (MQ=255) cagcaCAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTAcc < 1:226999/150‑1 (MQ=255) agcaCAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcg < 2:13224/150‑1 (MQ=255) cacaATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgac < 2:1563262/150‑1 (MQ=255) cacaATATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgac < 2:356884/150‑1 (MQ=255) acaATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcgacg > 1:123479/1‑150 (MQ=255) aaTATAAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGAt > 2:2651054/1‑150 (MQ=255) atatCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATc > 1:3952832/1‑150 (MQ=255) atatCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATc > 1:4574016/1‑150 (MQ=255) tatCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATcc < 2:633598/150‑1 (MQ=255) tatCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATcc < 2:3993936/150‑1 (MQ=255) cAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCa < 1:374763/150‑1 (MQ=255) cAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCa > 1:4196741/1‑150 (MQ=255) | TCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCA > CP009273/3216950‑3217247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |