Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,219,616 A→T 16.3% T8T (ACA→ACT)  ygjI → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,219,6160AT16.3% 795.2 / 128.3 286T8T (ACA→ACT) ygjIputative transporter
Reads supporting (aligned to +/- strand):  ref base A (124/115);  new base T (18/29);  total (142/144)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

AACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATC  >  CP009273/3219468‑3219763
                                                                                                                                                    |                                                                                                                                                   
aaCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACaa                                                                                                                                                    <  2:3189764/150‑1 (MQ=255)
  cgcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATc                                                                                                                                                  <  2:4093930/150‑1 (MQ=255)
  cgcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATc                                                                                                                                                  <  2:371221/150‑1 (MQ=255)
           aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTc                                                                                                                                         >  1:3545593/1‑150 (MQ=255)
           aaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTc                                                                                                                                         <  2:1695264/150‑1 (MQ=255)
               gTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCt                                                                                                                                     <  2:3949568/150‑1 (MQ=255)
               gTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCt                                                                                                                                     <  2:3213527/150‑1 (MQ=255)
                tGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCtt                                                                                                                                    <  1:3052475/150‑1 (MQ=255)
                   ttCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGct                                                                                                                                 <  1:4257163/150‑1 (MQ=255)
                    tctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGctc                                                                                                                                >  2:4110150/1‑150 (MQ=255)
                    tctcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGctc                                                                                                                                >  2:112026/1‑150 (MQ=255)
                      tcAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCtctc                                                                                                                              <  2:565660/150‑1 (MQ=255)
                       cAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCg                                                                                                                             >  2:1468779/1‑150 (MQ=255)
                         aaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCt                                                                                                                           <  2:4392591/150‑1 (MQ=255)
                          aGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTg                                                                                                                          <  1:1950992/150‑1 (MQ=255)
                             cACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACt                                                                                                                       <  2:3634798/150‑1 (MQ=255)
                             cACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACt                                                                                                                       >  2:107326/1‑150 (MQ=255)
                               ccATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACttt                                                                                                                     >  1:1095109/1‑150 (MQ=255)
                                 aTCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTg                                                                                                                   <  2:4554624/150‑1 (MQ=255)
                                   cGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTgcc                                                                                                                 <  1:3368316/150‑1 (MQ=255)
                                    gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTgccg                                                                                                                <  1:2354337/150‑1 (MQ=255)
                                     aaGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTgccgc                                                                                                               >  2:1576551/1‑150 (MQ=255)
                                      aGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTgccgcc                                                                                                              <  2:4168945/150‑1 (MQ=255)
                                      aGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTgccgcc                                                                                                              <  2:1757937/150‑1 (MQ=255)
                                       gATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGTAGTTTGCAGCCACCGCTACCCTACTCATTTTCGGAGAAGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGccgccg                                                                                                             >  1:2167180/1‑150 (MQ=255)
                                         tGGTTATTTCCATACCAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGtt                                                                                                           <  2:2116024/150‑1 (MQ=255)
                                         tGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGtt                                                                                                           <  1:3832585/150‑1 (MQ=255)
                                         tGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGtt                                                                                                           >  2:928791/1‑150 (MQ=255)
                                         tGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGtt                                                                                                           >  2:4529564/1‑150 (MQ=255)
                                             tATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACa                                                                                                       <  1:4167421/150‑1 (MQ=255)
                                             tATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACa                                                                                                       >  2:1671015/1‑150 (MQ=255)
                                                ttCCATAACAAATAACAACTACGGCGGCAAAAGNAGTTTGCCGNCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCt                                                                                                    <  1:3393172/150‑1 (MQ=255)
                                                ttCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCt                                                                                                    >  1:644845/1‑150 (MQ=255)
                                                   cATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTa                                                                                                 >  1:4495454/1‑150 (MQ=255)
                                                    ataacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTaa                                                                                                <  1:1612661/150‑1 (MQ=255)
                                                    ataacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTaa                                                                                                <  1:4644706/150‑1 (MQ=255)
                                                     taacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTaac                                                                                               >  1:3605978/1‑150 (MQ=255)
                                                      aacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTAATTTTAGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCACATTCGGCTTGCTCTCGCTGACTTTTGCCGACGTATACAGCTTTaaca                                                                                              >  2:1443053/1‑150 (MQ=255)
                                                                aacTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCaa                                                                                    >  2:2273683/1‑150 (MQ=255)
                                                                aacTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGGAATACTATCGGCAAATTCGGATTGCTCTCGCGGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCaa                                                                                    >  1:2218046/1‑150 (MQ=255)
                                                                        cggcAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAataataata                                                                            >  2:1191141/1‑150 (MQ=255)
                                                                          gcAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATAtt                                                                          <  2:2461622/150‑1 (MQ=255)
                                                                            aaaaGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGa                                                                        >  2:1818111/1‑150 (MQ=255)
                                                                            aaaaGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGa                                                                        >  2:4279603/1‑150 (MQ=255)
                                                                             aaaGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAg                                                                       <  2:2833375/150‑1 (MQ=255)
                                                                             aaaGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAg                                                                       >  1:315842/1‑150 (MQ=255)
                                                                             aaaGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAg                                                                       >  1:1684462/1‑150 (MQ=255)
                                                                               aGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCt                                                                     <  1:2431844/150‑1 (MQ=255)
                                                                                ggAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCtt                                                                    <  1:3439412/150‑1 (MQ=255)
                                                                                ggAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCtt                                                                    >  2:669212/1‑150 (MQ=255)
                                                                                  aGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTgg                                                                  <  1:1468779/150‑1 (MQ=255)
                                                                                   gTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTATCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGa                                                                 >  2:4498495/1‑150 (MQ=255)
                                                                                        ccgccACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGc                                                            <  2:1913927/150‑1 (MQ=255)
                                                                                        ccgccACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGc                                                            <  1:2364413/150‑1 (MQ=255)
                                                                                          gccACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCt                                                          <  2:790875/150‑1 (MQ=255)
                                                                                            cACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCg                                                        <  1:983673/150‑1 (MQ=255)
                                                                                             aCCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCgg                                                       >  2:4238047/1‑150 (MQ=255)
                                                                                                 cTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCAcc                                                   <  1:3022482/150‑1 (MQ=255)
                                                                                                 cTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCAcc                                                   <  1:2330543/150‑1 (MQ=255)
                                                                                                  tACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGACACCg                                                  >  2:12234/1‑150 (MQ=255)
                                                                                                  tACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGACACCg                                                  >  2:2322506/1‑150 (MQ=255)
                                                                                                   aCCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGa                                                 >  2:483852/1‑150 (MQ=255)
                                                                                                        aCTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGttt                                            >  2:1206404/1‑150 (MQ=255)
                                                                                                        aCTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGttt                                            >  2:1204613/1‑150 (MQ=255)
                                                                                                         cTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGtttt                                           <  2:4507075/150‑1 (MQ=255)
                                                                                                         cTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGtttt                                           >  1:3049361/1‑150 (MQ=255)
                                                                                                            aTTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTcc                                        >  2:3483589/1‑150 (MQ=255)
                                                                                                              tttCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTc                                      <  1:1161360/150‑1 (MQ=255)
                                                                                                              tttCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTc                                      <  2:3404468/150‑1 (MQ=255)
                                                                                                               ttCGGAGATGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTcg                                     <  1:2581362/150‑1 (MQ=255)
                                                                                                                 cGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTcgcg                                   >  2:1043278/1‑150 (MQ=255)
                                                                                                                    agaTGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCACTAATCAGATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGcgacg                                >  2:2033583/1‑150 (MQ=255)
                                                                                                                     gaTGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGcgacga                               <  2:1249066/150‑1 (MQ=255)
                                                                                                                      aTGTGTTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAt                              <  2:4020330/150‑1 (MQ=255)
                                                                                                                         tgtTATGTCTGATACCAAACGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAtttt                           <  1:1539669/150‑1 (MQ=255)
                                                                                                                         tgtTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAtttt                           <  1:2472852/150‑1 (MQ=255)
                                                                                                                          gtTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAttttt                          >  1:2066159/1‑150 (MQ=255)
                                                                                                                           ttATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAtttttt                         <  2:4287837/150‑1 (MQ=255)
                                                                                                                           ttATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGAtttttt                         <  2:1088144/150‑1 (MQ=255)
                                                                                                                            tATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTtttta                        <  2:3502619/150‑1 (MQ=255)
                                                                                                                            tATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTtttta                        >  1:2302043/1‑150 (MQ=255)
                                                                                                                            tATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTtttta                        >  1:3634835/1‑150 (MQ=255)
                                                                                                                               gTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTttttattt                     >  1:852194/1‑150 (MQ=255)
                                                                                                                                tCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTttttatttt                    <  1:1663282/150‑1 (MQ=255)
                                                                                                                                tCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTttttatttt                    <  1:3917900/150‑1 (MQ=255)
                                                                                                                                tCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTttttatttt                    >  1:2912549/1‑150 (MQ=255)
                                                                                                                                  tGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTtttattttat                  <  1:107326/150‑1 (MQ=255)
                                                                                                                                  tGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTtttattttat                  >  1:1445698/1‑150 (MQ=255)
                                                                                                                                    aTACCAAACGTAATCAAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTc                <  1:435150/150‑1 (MQ=255)
                                                                                                                                    aTACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTc                >  1:1732953/1‑150 (MQ=255)
                                                                                                                                     tACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTcc               <  1:366344/150‑1 (MQ=255)
                                                                                                                                       ccAACCGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCt             <  2:2366773/150‑1 (MQ=255)
                                                                                                                                         aaaCGTAATACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTtc           >  1:2972328/1‑150 (MQ=255)
                                                                                                                                          aaCGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTtct          <  1:2784977/150‑1 (MQ=255)
                                                                                                                                           aCGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTtctg         <  2:2749423/150‑1 (MQ=255)
                                                                                                                                           aCGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTtctg         <  1:1886817/150‑1 (MQ=255)
                                                                                                                                           aCGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTtctg         <  2:3759390/150‑1 (MQ=255)
                                                                                                                                            cGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTtctgt        <  1:1167659/150‑1 (MQ=255)
                                                                                                                                              tAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTtctgtct      <  1:714756/150‑1 (MQ=255)
                                                                                                                                               aaTACTATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTtctgtctg     <  1:328245/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATC  >  CP009273/3219468‑3219763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑