Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,245,848 G→T 100% L62L (CTG→CTT)  yhaH → DUF805 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,245,8480GT95.6% 843.1 / 26.0 266L62L (CTG→CTT) yhaHDUF805 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (8/4);  new base T (156/98);  total (164/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 268 total aligned reads displayed.

TTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGAAA  >  CP009273/3245701‑3245993
                                                                                                                                                   |                                                                                                                                                 
 ttCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTtgtg                                                                                                                                                >  2:3334849/1‑150 (MQ=255)
      gggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGttttt                                                                                                                                           <  2:1514526/150‑1 (MQ=255)
       ggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCTTGCTGGGGATGCTGGATAAAATGTTAGGCTGGCAAAGCGCTGGTGGCGAAGGCATCATGACGACAATATACGGTATTCTTGTGTATTTa                                                                                                                                          >  2:401414/1‑150 (MQ=255)
       ggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTa                                                                                                                                          <  1:2284399/150‑1 (MQ=255)
          cGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCg                                                                                                                                       <  1:4048661/150‑1 (MQ=255)
            tGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCgtg                                                                                                                                     >  2:1961981/1‑150 (MQ=255)
            tGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCgtg                                                                                                                                     <  2:403394/150‑1 (MQ=255)
            tGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCgtg                                                                                                                                     <  1:943045/150‑1 (MQ=255)
               gcggcgCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtggtg                                                                                                                                  >  2:1608840/1‑150 (MQ=255)
                    gcAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCgg                                                                                                                             >  2:2603684/1‑150 (MQ=255)
                     cAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGt                                                                                                                            >  2:1710798/1‑150 (MQ=255)
                      aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGtt                                                                                                                           >  1:44475/1‑150 (MQ=255)
                      aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGtt                                                                                                                           <  1:1215858/150‑1 (MQ=255)
                      aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGtt                                                                                                                           >  1:3613178/1‑150 (MQ=255)
                       aaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTc                                                                                                                          >  2:4108466/1‑150 (MQ=255)
                        agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCa                                                                                                                         >  1:3755669/1‑150 (MQ=255)
                         gagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTAGTCTTGTGTTTTTACCGTGGTGGGCGGTTCAg                                                                                                                        <  1:3847/150‑1 (MQ=255)
                           gTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGtt                                                                                                                      <  1:2856954/150‑1 (MQ=255)
                            tACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTc                                                                                                                     >  2:4561634/1‑150 (MQ=255)
                              cTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccg                                                                                                                   >  2:29137/1‑150 (MQ=255)
                                ggATGTTTATTCTGGTCAACATCATCTTTACGTTTGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgcc                                                                                                                 >  1:187230/1‑150 (MQ=255)
                                   tGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCgccgcc                                                                                                              >  2:3149931/1‑150 (MQ=255)
                                        aTTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCAc                                                                                                         <  2:2962853/150‑1 (MQ=255)
                                         ttCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACg                                                                                                        >  2:526112/1‑150 (MQ=255)
                                            tGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGaca                                                                                                     >  2:1789936/1‑150 (MQ=255)
                                            tGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGaca                                                                                                     >  2:4196842/1‑150 (MQ=255)
                                            tGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGaca                                                                                                     <  1:2573576/150‑1 (MQ=255)
                                               tCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccg                                                                                                  >  1:2919449/1‑150 (MQ=255)
                                               tCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccg                                                                                                  >  1:593697/1‑150 (MQ=255)
                                               tCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccg                                                                                                  >  2:2850275/1‑150 (MQ=255)
                                                cAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccga                                                                                                 >  2:4348646/1‑150 (MQ=255)
                                                cAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccga                                                                                                 >  1:4528754/1‑150 (MQ=255)
                                                 aaCATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccgac                                                                                                >  2:787273/1‑150 (MQ=255)
                                                  aCATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccgacc                                                                                               <  2:3554727/150‑1 (MQ=255)
                                                    atcatcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGc                                                                                             >  2:299372/1‑150 (MQ=255)
                                                      catcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTc                                                                                           >  2:3283953/1‑150 (MQ=255)
                                                           ttACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgt                                                                                      >  2:3625474/1‑150 (MQ=255)
                                                             aCGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtgg                                                                                    >  1:4281153/1‑150 (MQ=255)
                                                              cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggt                                                                                   >  2:2166319/1‑150 (MQ=255)
                                                               gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                  >  2:3218071/1‑150 (MQ=255)
                                                                     gCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACt                                                                            >  1:1079633/1‑150 (MQ=255)
                                                                     gCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACt                                                                            <  2:1498546/150‑1 (MQ=255)
                                                                       tGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGc                                                                          >  2:234299/1‑150 (MQ=255)
                                                                       tGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGc                                                                          >  2:2466481/1‑150 (MQ=255)
                                                                       tGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGAACGCTCGGCGTGGTGGGCACTGc                                                                          >  2:1223815/1‑150 (MQ=255)
                                                                        ggggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCt                                                                         >  1:4641373/1‑150 (MQ=255)
                                                                         gggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTa                                                                        >  1:2990527/1‑150 (MQ=255)
                                                                         gggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTa                                                                        >  1:1149989/1‑150 (MQ=255)
                                                                          ggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAt                                                                       <  1:3218071/150‑1 (MQ=255)
                                                                           gctgctGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAtt                                                                      >  1:2060665/1‑150 (MQ=255)
                                                                           gctgctGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAtt                                                                      >  2:4003929/1‑150 (MQ=255)
                                                                            ctgctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTc                                                                     <  1:3933641/150‑1 (MQ=255)
                                                                               ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTa                                                                  <  1:234528/150‑1 (MQ=255)
                                                                               ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTa                                                                  <  1:417277/150‑1 (MQ=255)
                                                                                 ggATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAAt                                                                <  2:4532548/150‑1 (MQ=255)
                                                                                       aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGtt                                                          >  2:4499918/1‑150 (MQ=255)
                                                                                        aTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGttt                                                         <  1:1003544/150‑1 (MQ=255)
                                                                                         tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTa                                                        <  2:1858203/150‑1 (MQ=255)
                                                                                          gTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTAt                                                       <  2:661053/150‑1 (MQ=255)
                                                                                           ttAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATc                                                      <  2:4281153/150‑1 (MQ=255)
                                                                                                cTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctg                                                 >  2:2457543/1‑150 (MQ=255)
                                                                                                cTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctg                                                 <  1:1039663/150‑1 (MQ=255)
                                                                                                 tGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctgg                                                >  2:8549/1‑150 (MQ=255)
                                                                                                  gtcAACGCGCTGGTGGCGAAGGCATAGTGACGACAATCTTCGGTTTTCTTGTTTTTTTTCTGGGGGGGGCGGTTCAGTTCCGCCGGCTGCACGTCTCCGACCGCTCGGCGTGGTGGGCACTTCTATTCTCAATCCCGTTTATCggctggc                                               <  1:2721621/148‑1 (MQ=255)
                                                                                                  ggCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctggc                                               >  1:833047/1‑150 (MQ=255)
                                                                                                     aaCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                                            >  1:4000507/1‑150 (MQ=255)
                                                                                                      aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttat                                           >  1:4459394/1‑150 (MQ=255)
                                                                                                       cgcgCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTATTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatt                                          <  2:4264013/150‑1 (MQ=255)
                                                                                                          gcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTTTTAATCCCGTTTATCGGCTGGCTTATTatc                                       >  2:4585733/1‑150 (MQ=255)
                                                                                                            tggtggCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatcat                                     <  1:4114608/150‑1 (MQ=255)
                                                                                                            tggtggCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatcat                                     >  1:2651116/1‑150 (MQ=255)
                                                                                                             ggtggCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatcatc                                    <  2:2556785/150‑1 (MQ=255)
                                                                                                              gtggCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCg                                   <  2:4615211/150‑1 (MQ=255)
                                                                                                                 gCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCt                                <  1:3334849/150‑1 (MQ=255)
                                                                                                                   gAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCttt                              >  1:3695818/1‑150 (MQ=255)
                                                                                                                   gAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCttt                              >  1:3695807/1‑150 (MQ=255)
                                                                                                                    aaGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTa                             >  2:2709939/1‑150 (MQ=255)
                                                                                                                     aGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTaa                            >  2:768342/1‑150 (MQ=255)
                                                                                                                        cATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTg                         >  1:502704/1‑150 (MQ=255)
                                                                                                                        cATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTg                         >  1:3390904/1‑150 (MQ=255)
                                                                                                                           ccTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCa                      >  2:3115556/1‑150 (MQ=255)
                                                                                                                           ccTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCa                      <  2:1317095/150‑1 (MQ=255)
                                                                                                                           ccTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCa                      <  1:2680554/150‑1 (MQ=255)
                                                                                                                            cTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAg                     >  2:136472/1‑150 (MQ=255)
                                                                                                                             tGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAgg                    <  1:2151431/150‑1 (MQ=255)
                                                                                                                              gacgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGc                   >  1:1646673/1‑150 (MQ=255)
                                                                                                                              gacgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGc                   >  1:1481202/1‑150 (MQ=255)
                                                                                                                               acgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCg                  <  1:1878140/150‑1 (MQ=255)
                                                                                                                                cgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCgg                 >  1:2027663/1‑150 (MQ=255)
                                                                                                                                cgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCgg                 >  1:4301340/1‑150 (MQ=255)
                                                                                                                                 gacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCggg                >  2:984497/1‑150 (MQ=255)
                                                                                                                                   cAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTa              >  1:643657/1‑150 (MQ=255)
                                                                                                                                    aaTCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTAc             <  2:4137080/150‑1 (MQ=255)
                                                                                                                                    aaTCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTAc             <  2:541806/150‑1 (MQ=255)
                                                                                                                                     aTCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACg            <  2:2047512/150‑1 (MQ=255)
                                                                                                                                     aTCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACg            >  1:16790/1‑150 (MQ=255)
                                                                                                                                       cTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGcc          <  2:1152543/150‑1 (MQ=255)
                                                                                                                                         aCGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAg        >  1:1401437/1‑150 (MQ=255)
                                                                                                                                          cGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAgg       <  1:1774119/150‑1 (MQ=255)
                                                                                                                                             tATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGa    <  1:3828217/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGAAA  >  CP009273/3245701‑3245993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑