BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,248,241	G→A	37.7%	G188G (GGG→GGA)	yhaK →	redox‑sensitive bicupin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,248,241	0	G	A	37.7%	296.8 / inf	286	G188G (GGG→GGA)	yhaK	redox‑sensitive bicupin
Reads supporting (aligned to +/- strand): ref base G (80/96); new base A (56/51); total (136/150)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01

Only 100 of 288 total aligned reads displayed.

AAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTA  >  CP009273/3248092‑3248388

aaGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACggg                                                                                                                                                     <  2:284160/150‑1 (MQ=255)

    agcaATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAAt                                                                                                                                                 >  1:2031595/1‑150 (MQ=255)

      caATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAAttt                                                                                                                                               <  1:1029515/150‑1 (MQ=255)

      caATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAAttt                                                                                                                                               >  2:1721930/1‑150 (MQ=255)

       aaTTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTc                                                                                                                                              >  2:2391081/1‑150 (MQ=255)

        aTTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCa                                                                                                                                             <  1:751210/150‑1 (MQ=255)

         ttAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGGAAGTGTGTCTGCACCATATCGTGCTCGACAAAGGCGTAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCAt                                                                                                                                            <  2:817988/150‑1 (MQ=255)

           aaTCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATgc                                                                                                                                          <  1:174983/150‑1 (MQ=255)

           aaTCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATgc                                                                                                                                          >  2:1377965/1‑150 (MQ=255)

              cgcctcgccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCt                                                                                                                                       >  2:1318897/1‑150 (MQ=255)

              cgcctcgccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCt                                                                                                                                       <  1:3737646/150‑1 (MQ=255)

               gcctcgccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTAGTTGCACTCGATTCACGGAAAATTTCATGCGCtt                                                                                                                                      >  2:4553673/1‑150 (MQ=255)

                cctcgccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTa                                                                                                                                     <  1:1643140/150‑1 (MQ=255)

                 ctcgccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTAc                                                                                                                                    <  1:488837/150‑1 (MQ=255)

                 ctcgccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTAc                                                                                                                                    >  1:3876221/1‑150 (MQ=255)

                    gccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGca                                                                                                                                 <  2:3092474/150‑1 (MQ=255)

                    gccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGca                                                                                                                                 <  2:266675/150‑1 (MQ=255)

                    gccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATGTCCAGTTGCATGGGCCACGCGCGTAGTTGCACTCGATTCACGGAAAATTTCATGCGCTTACGca                                                                                                                                 >  1:3444996/1‑150 (MQ=255)

                     ccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGcat                                                                                                                                <  2:1310088/150‑1 (MQ=255)

                     ccAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACAATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGcat                                                                                                                                <  2:583708/150‑1 (MQ=255)

                       agagGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGcatca                                                                                                                              >  1:336489/1‑150 (MQ=255)

                        gagGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGcatcat                                                                                                                             <  1:3193453/150‑1 (MQ=255)

                        gagGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGcatcat                                                                                                                             <  2:365482/150‑1 (MQ=255)

                         agGGTGCGATGGGAAGCCTGCAATTACGCCAGCTAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATg                                                                                                                            <  1:407563/150‑1 (MQ=255)

                         agGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATg                                                                                                                            >  1:1818815/1‑150 (MQ=255)

                         agGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATg                                                                                                                            <  2:4644570/150‑1 (MQ=255)

                         agGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCAAATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCAAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACTGGAAAATTCATGCGCTTACTCATAATg                                                                                                                            >  2:4278917/1‑150 (MQ=255)

                            gTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAag                                                                                                                         <  1:3616421/150‑1 (MQ=255)

                            gTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAag                                                                                                                         <  2:3000763/150‑1 (MQ=255)

                            gTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAag                                                                                                                         <  2:4206959/150‑1 (MQ=255)

                             tGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAaga                                                                                                                        <  2:692652/150‑1 (MQ=255)

                              gCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAagag                                                                                                                       >  1:854406/1‑150 (MQ=255)

                                gATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGaa                                                                                                                     <  1:596762/150‑1 (MQ=255)

                                 aTGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGaaa                                                                                                                    <  2:2586272/150‑1 (MQ=255)

                                 aTGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGaaa                                                                                                                    <  2:1459535/150‑1 (MQ=255)

                                  tGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAg                                                                                                                   <  1:1029199/150‑1 (MQ=255)

                                    ggATGCCTGCTATTACGACAGCAAGTGTGGCTGCACCATATCGTGCTCGACTAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGTTTCACGGGATATTTCATGCGCTTTCGCATCATGAAGAGAAAgcg                                                                                                                 <  1:2798769/150‑1 (MQ=255)

                                     gAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAgcgg                                                                                                                >  2:3428211/1‑150 (MQ=255)

                                      aaGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAgcggc                                                                                                               >  2:2408608/1‑150 (MQ=255)

                                       aGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAgcggcg                                                                                                              >  1:1351968/1‑150 (MQ=255)

                                          cTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTg                                                                                                           <  1:2253700/150‑1 (MQ=255)

                                               aTTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTg                                                                                                      >  2:1695145/1‑150 (MQ=255)

                                               aTTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTg                                                                                                      >  2:2155043/1‑150 (MQ=255)

                                                    gCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTg                                                                                                 <  1:2164989/150‑1 (MQ=255)

                                                     ccAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGa                                                                                                >  2:4531709/1‑150 (MQ=255)

                                                         cAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGTGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATggg                                                                                            >  1:4515706/1‑150 (MQ=255)

                                                         cAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATggg                                                                                            <  1:69146/150‑1 (MQ=255)

                                                         cAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATggg                                                                                            <  2:3966542/150‑1 (MQ=255)

                                                             tgtgGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGt                                                                                        >  2:702748/1‑150 (MQ=255)

                                                             tgtgGCTGCACCATATCGTGCTCGACAAAGACGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGt                                                                                        >  1:4290424/1‑150 (MQ=255)

                                                               tgGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGttt                                                                                      <  1:2900565/150‑1 (MQ=255)

                                                                ggCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTa                                                                                     <  1:3400256/150‑1 (MQ=255)

                                                                ggCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTa                                                                                     >  2:3166138/1‑150 (MQ=255)

                                                                ggCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTa                                                                                     <  1:2477094/150‑1 (MQ=255)

                                                                       ccATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGa                                                                              >  1:1579236/1‑150 (MQ=255)

                                                                          tatCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGa                                                                           >  1:3559545/1‑150 (MQ=255)

                                                                           atCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAg                                                                          <  1:807012/150‑1 (MQ=255)

                                                                            tCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAgg                                                                         >  1:4129208/1‑150 (MQ=255)

                                                                              gTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCt                                                                       <  2:1856729/150‑1 (MQ=255)

                                                                              gTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCt                                                                       >  2:3420274/1‑150 (MQ=255)

                                                                               tGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTa                                                                      <  2:4018474/150‑1 (MQ=255)

                                                                               tGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTa                                                                      <  2:1097123/150‑1 (MQ=255)

                                                                                      cAACGGGGAAAGTGCGAATTTCCAGTTGCTTGGGCCACGCGCGTATTTGCAATCGTTTCACGGGAAATTTCATGCGTTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTAc                                                               <  1:1719387/150‑1 (MQ=255)

                                                                                      cAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTAc                                                               >  1:4452817/1‑150 (MQ=255)

                                                                                       aaaGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACg                                                              >  2:3812330/1‑150 (MQ=255)

                                                                                         aGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCt                                                            <  1:2630950/150‑1 (MQ=255)

                                                                                         aGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCt                                                            >  1:1922398/1‑150 (MQ=255)

                                                                                            cGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGt                                                         >  1:4168290/1‑150 (MQ=255)

                                                                                             gAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGtt                                                        >  2:1051565/1‑150 (MQ=255)

                                                                                             gAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGtt                                                        <  2:1236472/150‑1 (MQ=255)

                                                                                                 gTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCg                                                    >  2:4347533/1‑150 (MQ=255)

                                                                                                  tGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGa                                                   <  1:3780792/150‑1 (MQ=255)

                                                                                                  tGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGa                                                   <  1:3780510/150‑1 (MQ=255)

                                                                                                      aaTTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTcc                                               >  2:3490408/1‑150 (MQ=255)

                                                                                                      aaTTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTcc                                               >  1:2261063/1‑150 (MQ=255)

                                                                                                      aaTTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTcc                                               >  1:116613/1‑150 (MQ=255)

                                                                                                      aaTTTACAGTTGCATGGGCCACGCGCGTATTTGCAATCCATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTcc                                               >  2:2889493/1‑150 (MQ=255)

                                                                                                       aTTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTccc                                              >  2:1622283/1‑150 (MQ=255)

                                                                                                        tttCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTcccc                                             <  1:4370725/150‑1 (MQ=255)

                                                                                                           ccAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACt                                          <  1:1318897/150‑1 (MQ=255)

                                                                                                           ccAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACt                                          >  2:4435419/1‑150 (MQ=255)

                                                                                                           ccAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACt                                          <  1:2127026/150‑1 (MQ=255)

                                                                                                           ccAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACt                                          <  2:1493618/150‑1 (MQ=255)

                                                                                                              gTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTgcg                                       <  1:3054867/150‑1 (MQ=255)

                                                                                                                tGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTgcgcg                                     <  2:2686234/150‑1 (MQ=255)

                                                                                                                  cATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCt                                   >  2:326474/1‑150 (MQ=255)

                                                                                                                  cATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCt                                   <  2:3939583/150‑1 (MQ=255)

                                                                                                                   aTGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCtt                                  >  2:661398/1‑150 (MQ=255)

                                                                                                                      gggcACGCGCGTATTTGCACTAGATTCACGGGAAATTTCAGGCGCTTACGAATCATGAAGTGAATGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCCCTGCGCGCTTTGc                               <  2:576861/147‑1 (MQ=255)

                                                                                                                      ggCCACGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGc                               >  1:3669551/1‑150 (MQ=255)

                                                                                                                        ccACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTg                             <  2:2774308/150‑1 (MQ=255)

                                                                                                                         cACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGa                            >  1:3948798/1‑150 (MQ=255)

                                                                                                                          aCGCGCGTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGAt                           <  2:1970299/150‑1 (MQ=255)

                                                                                                                           cgcgcgTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATa                          >  2:4313533/1‑150 (MQ=255)

                                                                                                                            gcgcgTATTTGCAATCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAg                         >  1:1580357/1‑150 (MQ=255)

                                                                                                                                        aaTCGATTCACGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCTATTCCCCACTGCGCGCTTTGCTGATAGATTTGACTGTCt             >  2:4586412/1‑150 (MQ=255)

                                                                                                                                            gATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAgtt         >  1:3257009/1‑150 (MQ=255)

                                                                                                                                                 aCGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGtttt    >  1:3057469/1‑150 (MQ=255)

                                                                                                                                                 aCGGAAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGtttt    <  2:2612180/150‑1 (MQ=255)

                                                                                                                                                  cGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGttttt   <  1:1389863/150‑1 (MQ=255)

AAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCACGGGAAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTA  >  CP009273/3248092‑3248388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`