Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,292,182 G→T 14.9% G8G (GGC→GGA)  yraR ← putative nucleoside‑diphosphate‑sugar epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,292,1820GT14.9% 984.5 / 100.2 294G8G (GGC→GGA) yraRputative nucleoside‑diphosphate‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base G (118/132);  new base T (17/27);  total (135/159)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 296 total aligned reads displayed.

CGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAACGCGGAGGAA  >  CP009273/3292035‑3292330
                                                                                                                                                   |                                                                                                                                                    
 gcCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCt                                                                                                                                                   <  1:232961/150‑1 (MQ=255)
  ccAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTg                                                                                                                                                  <  1:3765563/150‑1 (MQ=255)
     aCGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTaa                                                                                                                                               <  1:4437401/150‑1 (MQ=255)
     aCGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTaa                                                                                                                                               <  2:1978529/150‑1 (MQ=255)
      cgcgTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAAt                                                                                                                                              <  1:3214006/150‑1 (MQ=255)
      cgcgTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAAt                                                                                                                                              >  1:1361810/1‑150 (MQ=255)
       gcgTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATc                                                                                                                                             <  1:491625/150‑1 (MQ=255)
        cgTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCa                                                                                                                                            <  1:2566785/150‑1 (MQ=255)
          tCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGt                                                                                                                                          <  1:571062/150‑1 (MQ=255)
           cACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTa                                                                                                                                         <  2:1207064/150‑1 (MQ=255)
           cACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTa                                                                                                                                         >  1:4141546/1‑150 (MQ=255)
            aCTCAGTTGCGGATCATGGGGATTTAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTAc                                                                                                                                        <  1:1067638/150‑1 (MQ=255)
             cTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTAcc                                                                                                                                       >  1:2651922/1‑150 (MQ=255)
               cAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTg                                                                                                                                     <  1:3231801/150‑1 (MQ=255)
                 gTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGAc                                                                                                                                   <  2:2715366/150‑1 (MQ=255)
                     cGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCAt                                                                                                                               >  2:671948/1‑150 (MQ=255)
                       gATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCt                                                                                                                             <  1:3068377/150‑1 (MQ=255)
                        aTCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCtt                                                                                                                            >  1:2941431/1‑150 (MQ=255)
                        aTCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCtt                                                                                                                            >  1:2783932/1‑150 (MQ=255)
                         tCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTc                                                                                                                           <  1:1267786/150‑1 (MQ=255)
                         tCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTc                                                                                                                           <  2:2916532/150‑1 (MQ=255)
                         tCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTc                                                                                                                           >  1:1471817/1‑150 (MQ=255)
                          cATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCg                                                                                                                          <  2:1313635/150‑1 (MQ=255)
                             ggggATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTc                                                                                                                       <  2:2363074/150‑1 (MQ=255)
                             ggggATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTc                                                                                                                       >  2:2784529/1‑150 (MQ=255)
                             ggggATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTc                                                                                                                       >  1:2364408/1‑150 (MQ=255)
                               ggATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCt                                                                                                                     >  2:3334159/1‑150 (MQ=255)
                               ggATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCt                                                                                                                     <  1:248849/150‑1 (MQ=255)
                               ggATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCt                                                                                                                     <  1:20738/150‑1 (MQ=255)
                               ggATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCt                                                                                                                     <  1:3390218/150‑1 (MQ=255)
                                gATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCtt                                                                                                                    <  1:4629028/150‑1 (MQ=255)
                                 aTTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCttt                                                                                                                   >  2:143036/1‑150 (MQ=255)
                                 aTTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCttt                                                                                                                   <  1:328074/150‑1 (MQ=255)
                                  ttAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTa                                                                                                                  <  1:2255624/150‑1 (MQ=255)
                                  ttAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTa                                                                                                                  <  2:3694943/150‑1 (MQ=255)
                                     aaCACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAg                                                                                                               <  2:1525039/150‑1 (MQ=255)
                                        acCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAAt                                                                                                            >  2:2401862/1‑150 (MQ=255)
                                          cccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTg                                                                                                          <  2:3788119/150‑1 (MQ=255)
                                            cGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTc                                                                                                        <  1:219914/150‑1 (MQ=255)
                                             gggCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCt                                                                                                       <  1:3385298/150‑1 (MQ=255)
                                                     cGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcg                                                                                               <  2:3610192/150‑1 (MQ=255)
                                                      gCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcgc                                                                                              >  2:2793069/1‑150 (MQ=255)
                                                        ccAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTc                                                                                            >  1:4310768/1‑150 (MQ=255)
                                                         cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTcc                                                                                           <  2:615848/150‑1 (MQ=255)
                                                          aaCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCa                                                                                          >  1:229746/1‑150 (MQ=255)
                                                          aaCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCa                                                                                          >  1:229443/1‑150 (MQ=255)
                                                          aaCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCa                                                                                          <  1:648516/150‑1 (MQ=255)
                                                           aCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCAc                                                                                         >  1:157484/1‑150 (MQ=255)
                                                            cGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACg                                                                                        >  1:846724/1‑150 (MQ=255)
                                                              gACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                                                                                      >  2:2646444/1‑150 (MQ=255)
                                                              gACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                                                                                      >  1:1922633/1‑150 (MQ=255)
                                                                  tcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt                                                                                  <  2:2526417/150‑1 (MQ=255)
                                                                    gcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAtac                                                                                <  2:4353363/150‑3 (MQ=255)
                                                                     cgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCa                                                                               >  2:2456288/1‑150 (MQ=255)
                                                                      gtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAc                                                                              >  1:679051/1‑150 (MQ=255)
                                                                      gtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAc                                                                              >  1:3495634/1‑150 (MQ=255)
                                                                        cgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCa                                                                            >  2:3016112/1‑150 (MQ=255)
                                                                           cgcAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGc                                                                         <  1:1673381/150‑1 (MQ=255)
                                                                            gcagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCt                                                                        <  2:410896/150‑1 (MQ=255)
                                                                              agcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTa                                                                      >  1:3153763/1‑150 (MQ=255)
                                                                              agcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTa                                                                      <  1:1846190/150‑1 (MQ=255)
                                                                              agcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTa                                                                      <  1:3336587/150‑1 (MQ=255)
                                                                                caATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAt                                                                    >  2:3573012/1‑150 (MQ=255)
                                                                                caATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAt                                                                    <  2:3397641/150‑1 (MQ=255)
                                                                                 aaTGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATa                                                                   <  1:923610/150‑1 (MQ=255)
                                                                                 aaTGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATa                                                                   <  1:3223838/150‑1 (MQ=255)
                                                                                      cGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCa                                                              >  1:1541121/1‑150 (MQ=255)
                                                                                      cGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCa                                                              <  1:1590869/150‑1 (MQ=255)
                                                                                      cGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCa                                                              <  1:1757282/150‑1 (MQ=255)
                                                                                             tttCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTa                                                       <  2:4400992/150‑1 (MQ=255)
                                                                                                cGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAAtt                                                    >  2:1225192/1‑150 (MQ=255)
                                                                                                   ttCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATc                                                 <  2:3198805/150‑1 (MQ=255)
                                                                                                      gTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATcccc                                              <  2:2066959/150‑1 (MQ=255)
                                                                                                      gTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATcccc                                              <  2:460703/150‑1 (MQ=255)
                                                                                                       ttAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCg                                             <  2:3092705/150‑1 (MQ=255)
                                                                                                       ttAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCg                                             >  1:2839770/1‑150 (MQ=255)
                                                                                                        tAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGa                                            >  1:4001573/1‑150 (MQ=255)
                                                                                                          aTCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGAtt                                          <  1:1745524/150‑1 (MQ=255)
                                                                                                             aaCATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTGTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATtgtg                                       >  2:3758631/1‑150 (MQ=255)
                                                                                                              aCATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTgg                                      <  1:87796/150‑1 (MQ=255)
                                                                                                               cATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTggg                                     >  2:955485/1‑150 (MQ=255)
                                                                                                                aTCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGa                                    <  2:2294372/150‑1 (MQ=255)
                                                                                                                aTCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGa                                    <  2:174605/150‑1 (MQ=255)
                                                                                                                aTCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGa                                    >  1:2216580/1‑150 (MQ=255)
                                                                                                                 tCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGaa                                   <  2:2808959/150‑1 (MQ=255)
                                                                                                                 tCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGaa                                   <  2:1004592/150‑1 (MQ=255)
                                                                                                                 tCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGaa                                   <  2:3983072/150‑1 (MQ=255)
                                                                                                                  ccGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGaaa                                  <  2:3851863/150‑1 (MQ=255)
                                                                                                                     cagcagGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTc                               >  1:1779808/1‑150 (MQ=255)
                                                                                                                     cagcagGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTc                               <  1:3590097/150‑1 (MQ=255)
                                                                                                                     cagcagGTGACAGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCAACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTc                               <  1:1005354/150‑1 (MQ=255)
                                                                                                                      agcagGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCg                              >  2:3489522/1‑150 (MQ=255)
                                                                                                                         agGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCt                           <  1:143036/150‑1 (MQ=255)
                                                                                                                                cGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCaa                    >  1:472663/1‑150 (MQ=255)
                                                                                                                                cGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCaa                    >  2:775597/1‑150 (MQ=255)
                                                                                                                                    caccaGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGca                <  2:3919452/150‑1 (MQ=255)
                                                                                                                                         gCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCaacaac           <  1:4173212/150‑1 (MQ=255)
                                                                                                                                          ccccGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAAcg          >  1:2106318/1‑150 (MQ=255)
                                                                                                                                           cccGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAAcgc         >  1:2721551/1‑150 (MQ=255)
                                                                                                                                                  cgcCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAACGCGGAGGaa  <  1:3434354/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAACGCGGAGGAA  >  CP009273/3292035‑3292330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑