BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,292,914	C→A	55.6%	D121Y (GAT→TAT)	yhbP ←	UPF0306 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,292,914	0	C	A	55.6%	‑3.5 / inf	288	D121Y (GAT→TAT)	yhbP	UPF0306 family protein
Reads supporting (aligned to +/- strand): ref base C (68/60); new base A (91/69); total (159/129)
Fisher's exact test for biased strand distribution p-value = 5.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

TTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCG  >  CP009273/3292766‑3293063

    tAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGcc                                                                                                                                                  <  2:3588969/150‑1 (MQ=255)

    tAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGcc                                                                                                                                                  >  2:3773571/1‑150 (MQ=255)

    tAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGcc                                                                                                                                                  >  1:885686/1‑150 (MQ=255)

     aaaGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCg                                                                                                                                                 >  1:3589370/1‑150 (MQ=255)

       aGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGa                                                                                                                                               >  2:2703053/1‑150 (MQ=255)

       aGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGa                                                                                                                                               <  1:1894073/150‑1 (MQ=255)

        gATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGAt                                                                                                                                              >  1:1783124/1‑150 (MQ=255)

           cAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTc                                                                                                                                           >  1:649591/1‑150 (MQ=255)

           cAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTc                                                                                                                                           >  2:814765/1‑150 (MQ=255)

            aGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTcc                                                                                                                                          >  2:2474878/1‑150 (MQ=255)

              cTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCca                                                                                                                                        >  1:2659794/1‑150 (MQ=255)

                  ttACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACAcc                                                                                                                                    <  1:4518323/150‑1 (MQ=255)

                   tACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCg                                                                                                                                   >  1:942145/1‑150 (MQ=255)

                   tACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCg                                                                                                                                   >  2:3033025/1‑150 (MQ=255)

                     ccAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCggg                                                                                                                                 <  2:784742/150‑1 (MQ=255)

                       aGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGc                                                                                                                               >  2:584160/1‑150 (MQ=255)

                       aGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGc                                                                                                                               >  2:2938196/1‑150 (MQ=255)

                        gCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGcc                                                                                                                              <  1:3423020/150‑1 (MQ=255)

                         ccGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCg                                                                                                                             <  1:2760824/150‑1 (MQ=255)

                           ggACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGAc                                                                                                                           >  1:1000957/1‑150 (MQ=255)

                            gACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACa                                                                                                                          >  1:3992556/1‑150 (MQ=255)

                             acacCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAg                                                                                                                         >  2:4449789/1‑150 (MQ=255)

                                  ggACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTc                                                                                                                    >  2:1994780/1‑150 (MQ=255)

                                    aCGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTg                                                                                                                  <  2:4525602/150‑1 (MQ=255)

                                     cGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTgg                                                                                                                 >  2:4280830/1‑150 (MQ=255)

                                     cGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTgg                                                                                                                 <  2:3568565/150‑1 (MQ=255)

                                     cGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTgg                                                                                                                 <  2:928831/150‑1 (MQ=255)

                                         cTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAAc                                                                                                             >  1:210623/1‑150 (MQ=255)

                                           gCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCg                                                                                                           <  2:1482556/150‑1 (MQ=255)

                                             cAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGa                                                                                                         >  1:3809228/1‑150 (MQ=255)

                                                cGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAg                                                                                                      <  1:227242/150‑1 (MQ=255)

                                                 gTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGc                                                                                                     <  2:1944529/150‑1 (MQ=255)

                                                   ttAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGTATTTGATTTCATCGAGCCGTATTTCCCACACCGGTGCCGACAGCAATCTGGCAACCGGAAAGcga                                                                                                   >  1:1479223/1‑150 (MQ=255)

                                                   ttAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGcga                                                                                                   >  2:3679967/1‑150 (MQ=255)

                                                    tAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGcgac                                                                                                  <  1:3278956/150‑1 (MQ=255)

                                                     aaCCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGACGCGTAGCCAAAACATTTTTTTACCAAAGCCCAGCGGGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCACAAACCTGGGCCGACAGCATTCTGGCAACCGGAAAGcgacg                                                                                                 >  1:3872767/1‑150 (MQ=255)

                                                      aCCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGcgacga                                                                                                >  2:3153835/1‑150 (MQ=255)

                                                         gcgAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTg                                                                                             >  2:62967/1‑150 (MQ=255)

                                                         gcgAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTg                                                                                             <  1:1070637/150‑1 (MQ=255)

                                                             ggCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACg                                                                                         >  2:478758/1‑150 (MQ=255)

                                                               cGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAACGCGACGATTGTACGcc                                                                                       <  2:140209/150‑1 (MQ=255)

                                                                gTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCt                                                                                      <  2:859563/150‑1 (MQ=255)

                                                                 ttACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCtt                                                                                     >  1:3823958/1‑150 (MQ=255)

                                                                 ttACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCtt                                                                                     >  2:1215628/1‑150 (MQ=255)

                                                                  tACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTg                                                                                    <  1:2405385/150‑1 (MQ=255)

                                                                        tGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGcgcgcg                                                                              <  2:3166974/150‑1 (MQ=255)

                                                                          cTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAg                                                                            >  1:800486/1‑150 (MQ=255)

                                                                           tCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAgg                                                                           >  2:3450309/1‑150 (MQ=255)

                                                                             ggTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTc                                                                         >  2:2373668/1‑150 (MQ=255)

                                                                               tGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGc                                                                       >  1:2996205/1‑150 (MQ=255)

                                                                                 ccTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCtt                                                                     <  2:3049376/150‑1 (MQ=255)

                                                                                 ccTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCtt                                                                     <  2:1806538/150‑1 (MQ=255)

                                                                                  cTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCttt                                                                    >  1:833406/1‑150 (MQ=255)

                                                                                   tGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTc                                                                   >  2:3683601/1‑150 (MQ=255)

                                                                                   tGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTc                                                                   >  2:2739980/1‑150 (MQ=255)

                                                                                     aGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCt                                                                 >  2:4351770/1‑150 (MQ=255)

                                                                                       tCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCa                                                               >  1:840388/1‑150 (MQ=255)

                                                                                        cgcgTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCAc                                                              <  1:1839578/150‑1 (MQ=255)

                                                                                           gTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCtt                                                           >  2:310204/1‑150 (MQ=255)

                                                                                            tAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTc                                                          >  2:1279038/1‑150 (MQ=255)

                                                                                                 aaaTCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGcc                                                     >  2:2236214/1‑150 (MQ=255)

                                                                                                 aaaTCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGcc                                                     >  2:4387635/1‑150 (MQ=255)

                                                                                                   aTCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTg                                                   >  2:397628/1‑150 (MQ=255)

                                                                                                    tCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGc                                                  <  2:4166018/150‑1 (MQ=255)

                                                                                                     cATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCTGATTTCCCACACCGTGGCCGACAGCATTCTTGCAACCGGAAAGCGACGATTGTACTCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCg                                                 >  2:2971150/1‑150 (MQ=255)

                                                                                                              aCCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGTCAACCGGAAAGCGACGATTGTACGCCTTGCGCTCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCAcc                                        >  2:1419913/1‑150 (MQ=255)

                                                                                                               ccAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCt                                       <  1:1984386/150‑1 (MQ=255)

                                                                                                               ccAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCt                                       >  1:1515057/1‑150 (MQ=255)

                                                                                                               ccAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCt                                       >  2:3084765/1‑150 (MQ=255)

                                                                                                               ccAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCt                                       <  1:2834255/150‑1 (MQ=255)

                                                                                                               ccAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCt                                       >  1:4415792/1‑150 (MQ=255)

                                                                                                               ccAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCt                                       <  2:1377154/150‑1 (MQ=255)

                                                                                                                   aGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTaa                                   >  1:1281688/1‑150 (MQ=255)

                                                                                                                   aGCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTaa                                   >  1:4254123/1‑150 (MQ=255)

                                                                                                                    gCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTaaa                                  <  1:2158650/150‑1 (MQ=255)

                                                                                                                    gCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTaaa                                  <  1:232747/150‑1 (MQ=255)

                                                                                                                    gCCCAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTaaa                                  >  1:1601841/1‑150 (MQ=255)

                                                                                                                     cccAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAAc                                 <  1:2588800/150‑1 (MQ=255)

                                                                                                                     cccAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTACCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAAc                                 <  1:1191739/150‑1 (MQ=255)

                                                                                                                      ccAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACt                                <  1:3447316/150‑1 (MQ=255)

                                                                                                                       cAGCGTGTTGTCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTg                               >  1:4157812/1‑150 (MQ=255)

                                                                                                                        aGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGc                              <  1:982594/150‑1 (MQ=255)

                                                                                                                            tgttgtCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCacac                          <  2:3341077/150‑1 (MQ=255)

                                                                                                                             gttgtCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACAcc                         >  1:3523042/1‑150 (MQ=255)

                                                                                                                             gttgtCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACAcc                         <  2:4188292/150‑1 (MQ=255)

                                                                                                                              ttgtCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACcg                        >  2:1860830/1‑150 (MQ=255)

                                                                                                                              ttgtCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACcg                        <  1:1412766/150‑1 (MQ=255)

                                                                                                                               tgtCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACcgc                       >  2:1879003/1‑150 (MQ=255)

                                                                                                                               tgtCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACcgc                       >  2:1601383/1‑150 (MQ=255)

                                                                                                                               tgtCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACcgc                       >  1:3466941/1‑150 (MQ=255)

                                                                                                                                gtCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACcgcg                      <  2:2087145/150‑1 (MQ=255)

                                                                                                                                gtCGGTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACcgcg                      <  2:112497/150‑1 (MQ=255)

                                                                                                                                   ggTGAATTTGATTTCATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAAt                   <  1:2465252/150‑1 (MQ=255)

                                                                                                                                    gTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAAtt                  <  2:4010065/150‑1 (MQ=255)

                                                                                                                                         tttGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGc             >  2:4141983/1‑150 (MQ=255)

                                                                                                                                           tGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTa           <  1:1203148/150‑1 (MQ=255)

                                                                                                                                            gATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTAc          <  2:331798/150‑1 (MQ=255)

                                                                                                                                             aTTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTAcc         >  2:4631358/1‑150 (MQ=255)

                                                                                                                                               ttCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGt       >  2:2530359/1‑150 (MQ=255)

                                                                                                                                                 cATAGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGttt     <  2:3355800/150‑1 (MQ=255)

TTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCG  >  CP009273/3292766‑3293063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`