New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 3330820	316 (1.130)	3 (0.010)	3/256	NT	1.1%	coding (431/636 nt)	mlaC	ABC transporter maintaining OM lipid asymmetry, periplasmic binding protein
	?	CP009273	3331036 =	249 (0.960)					coding (215/636 nt)	mlaC	ABC transporter maintaining OM lipid asymmetry, periplasmic binding protein
Rejected: Frequency below/above cutoff threshold.

GCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGCCACTGGAAGTCCAGACGCACCGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/3330665‑3330820 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcaccggcgTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTCTG  >  CP009273/3331036‑3331181                                                                                                                                                                                                                                                                                                                GCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGCCACTGGAAGTCCAGACGC                                                                                                                                                           >  1:2828332/1‑149    CAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGCCACTGGAAGTCCAGACGCACC                                                                                                                                                        <  2:4130424/149‑1    CAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGCCACTGGAAGTCCAGACGCACC                                                                                                                                                        <  1:3724002/149‑1    CAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGCCACTGGAAGTCCAGACGCACC                                                                                                                                                        <  1:1171510/149‑1    CAGTCAGGCCGTCGATACCTTGGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGCCACTGGAAGTCCAGACGCACC                                                                                                                                                        <  2:3871553/149‑1                                                                       tgttaaGCCGTCAGGACTCATTTCTTTAGCCAGCCACTTGCCCGGCCGGGAGTTTTTACGCCACTGGAAGTCCAGTCGCACCGGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTG                                                                                     <  2:2385372/143‑1                                                                                  CAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGCCACTGGAAGTCCAGACGCACCGGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTG                                                                          >  1:2200506/1‑149                                                                                                         CAATTGCCCGTCTGGGAGTTTTTACGCCACTGGAAGTCCAGACGCACCGGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGAT                                                   <  2:2200506/149‑1                                                                                                                                                   CGCACCGGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGAT         <  1:1604332/149‑1                                                                                                                                                   CGCACCGGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGAT         <  1:2000055/149‑1                                                                                                                                                      ACCGGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGG      >  1:3093851/1‑149                                                                                                                                                       CCGGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGT     >  1:271175/1‑149                                                                                                                                                       CCGGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGT     >  1:271049/1‑149                                                                                                                                                        CGGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTC    >  1:2473491/1‑149                                                                                                                                                         GGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTCT   <  2:3842219/149‑1                                                                                                                                                         GGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTCT   <  2:3841499/149‑1                                                                                                                                                         GGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTCT   <  2:4217486/149‑1                                                                                                                                                         GGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTCT   <  2:2492632/149‑1                                                                                                                                                         GGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTCT   <  2:2029701/149‑1                                                                                                                                                         GGCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTCT   >  2:1357624/1‑149                                                                                                                                                          GCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACGATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTCTG  >  1:4127917/1‑149                                                                                                                                                          GCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACGATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTCTG  >  1:4127882/1‑149                                                                                                                                                          GCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGACCGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGCTTGGTCTG  >  2:3474252/1‑149                                                                                                                                                          GCGTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTCTG  >  1:3335169/1‑149                                                                                                                                                                                                                                                                                                                GCGCAGTCAGGCCGTCGATACCTTTGGTACGCAGCAGCGTTCCCCACTCGTTTTGTTTGGTGGTGATCATACTGACGCCTTCAGCAATCATGTCGTAAGCCTGCCAATTGCCCGTCTGGGAGTTTTTACGCCACTGGAAGTCCAGACGCACCGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/3330665‑3330820 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcaccggcgTATTTCACCTGTACGTATGGCAGCAGTTCCTGATCAACAATGGTACGCAGATAATCCGGGTTGGCCCGAATTTGCGGTTGCTCATTCTTCAGGCGATCGAACGTTTTCTGCGCCGCCTCGTCCATCAGCTTATACGGATTGGTCTG  >  CP009273/3331036‑3331181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.