Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 29,139 G→T 100% G256C (GGT→TGT)  dapB → dihydrodipicolinate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927329,1390GT99.4% 1060.7 / ‑2.2 312G256C (GGT→TGT) dapBdihydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (121/189);  total (123/189)
Fisher's exact test for biased strand distribution p-value = 1.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 312 total aligned reads displayed.

GTTTTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGGTGT  >  CP009273/28993‑29284
                                                                                                                                                  |                                                                                                                                                 
gTTTTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGt                                                                                                                                                 >  2:638174/1‑149 (MQ=255)
 ttttGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTa                                                                                                                                                >  1:2196207/1‑149 (MQ=255)
        aCCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAg                                                                                                                                         <  2:1411809/149‑1 (MQ=255)
          cgtgcgtgCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCg                                                                                                                                       >  2:339403/1‑149 (MQ=255)
             gcgtgcAGGTGACATCGTTGGTGAACATACCTCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTc                                                                                                                                    <  2:1132659/149‑1 (MQ=255)
              cgtgcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCt                                                                                                                                   <  2:3454165/149‑1 (MQ=255)
               gtgcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCtt                                                                                                                                  <  2:2875754/149‑1 (MQ=255)
               gtgcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCtt                                                                                                                                  <  1:4422175/149‑1 (MQ=255)
                tgcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCttt                                                                                                                                 <  1:1601424/149‑1 (MQ=255)
                 gcAGGTGACATCGTTGGTGAACATACCGCGTTGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCtttt                                                                                                                                <  1:3227589/149‑1 (MQ=255)
                    ggTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGa                                                                                                                             >  1:1708481/1‑149 (MQ=255)
                    ggTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGa                                                                                                                             >  1:4664204/1‑149 (MQ=255)
                    ggTGACATCGTTGGTGAACATACCGCGATGTGTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGa                                                                                                                             <  2:2450171/149‑1 (MQ=255)
                     gTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGat                                                                                                                            <  1:2075986/149‑1 (MQ=255)
                      tGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGata                                                                                                                           >  2:2792501/1‑149 (MQ=255)
                      tGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGata                                                                                                                           >  2:326718/1‑149 (MQ=255)
                      tGACATCGTTGGTGAACATACCGCGATGTGTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGata                                                                                                                           <  2:1055095/149‑1 (MQ=255)
                         cATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGc                                                                                                                        >  1:1808591/1‑149 (MQ=255)
                         cATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGc                                                                                                                        <  1:84679/149‑1 (MQ=255)
                              ttGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGAt                                                                                                                   >  2:1762996/1‑149 (MQ=255)
                              ttGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGAt                                                                                                                   <  2:1352480/149‑1 (MQ=255)
                              ttGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGAt                                                                                                                   >  2:2992837/1‑149 (MQ=255)
                                ggTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGt                                                                                                                 >  1:107814/1‑149 (MQ=255)
                                  tGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTAc                                                                                                               >  2:2804410/1‑149 (MQ=255)
                                   gAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACt                                                                                                              >  2:3967527/1‑149 (MQ=255)
                                   gAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACt                                                                                                              <  1:3880730/149‑1 (MQ=255)
                                    aaCATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACtt                                                                                                             >  1:3531590/1‑149 (MQ=255)
                                        tACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGAtc                                                                                                         <  1:3653845/149‑1 (MQ=255)
                                        tACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACCTgctg                                                                                                         >  2:2349044/1‑146 (MQ=255)
                                            gcgATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCaa                                                                                                     >  2:4667549/1‑149 (MQ=255)
                                              gATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCaata                                                                                                   >  2:89351/1‑149 (MQ=255)
                                               aTGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTGTTTGATATGCGAGATGTACTTGATCTCaataa                                                                                                  >  1:406861/1‑149 (MQ=255)
                                                   ttGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTg                                                                                              <  2:2157213/149‑1 (MQ=255)
                                                     gCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTa                                                                                            >  1:4698998/1‑149 (MQ=255)
                                                      ccGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTaa                                                                                           <  2:2875103/149‑1 (MQ=255)
                                                      ccGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTaa                                                                                           >  1:4566285/1‑149 (MQ=255)
                                                        gATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAAcc                                                                                         <  1:1348548/149‑1 (MQ=255)
                                                           atTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAAAcaca                                                                                      >  2:689913/1‑149 (MQ=255)
                                                            ttGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACaa                                                                                     >  2:47520/1‑149 (MQ=255)
                                                              ggCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACaaaa                                                                                   >  2:4231568/1‑149 (MQ=255)
                                                               gCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAat                                                                                  <  1:2289883/149‑1 (MQ=255)
                                                                cGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAata                                                                                 <  1:2856754/149‑1 (MQ=255)
                                                                cGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAata                                                                                 <  1:2167828/149‑1 (MQ=255)
                                                                      tCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGtt                                                                           <  1:3815429/149‑1 (MQ=255)
                                                                        tGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTAt                                                                         <  2:4125512/149‑1 (MQ=255)
                                                                        tGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTAt                                                                         >  1:96433/1‑149 (MQ=255)
                                                                         ggAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATg                                                                        <  1:3341987/149‑1 (MQ=255)
                                                                            gaTCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTg                                                                     >  1:3357122/1‑149 (MQ=255)
                                                                            gaTCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTg                                                                     <  1:4783904/149‑1 (MQ=255)
                                                                             aTCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGc                                                                    >  1:1313687/1‑149 (MQ=255)
                                                                               cACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCaa                                                                  <  1:396347/149‑1 (MQ=255)
                                                                                aCCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCaaa                                                                 >  1:4122153/1‑149 (MQ=255)
                                                                                  ccATAAGGCGTCCAGCCGTATGACAGTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCaaaaa                                                               <  2:3198587/149‑1 (MQ=255)
                                                                                      aaGGCGTCCAGCCGTATGACTTTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAac                                                           <  1:3399335/149‑1 (MQ=255)
                                                                                      aaGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAac                                                           <  1:4720081/149‑1 (MQ=255)
                                                                                      aaGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAac                                                           <  1:1483239/149‑1 (MQ=255)
                                                                                        ggCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAacac                                                         >  1:2323533/1‑149 (MQ=255)
                                                                                        ggCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAacac                                                         <  2:1323241/149‑1 (MQ=255)
                                                                                        ggCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAacac                                                         <  2:4371864/149‑1 (MQ=255)
                                                                                        ggCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAacac                                                         >  1:2844202/1‑149 (MQ=255)
                                                                                            tCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACattt                                                     >  1:2526350/1‑149 (MQ=255)
                                                                                            tCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACattt                                                     <  1:1174701/149‑1 (MQ=255)
                                                                                            tCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACattt                                                     <  1:2812963/149‑1 (MQ=255)
                                                                                            tCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACattt                                                     <  1:1009531/149‑1 (MQ=255)
                                                                                              cAGCCTTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaa                                                   <  1:3450737/149‑1 (MQ=255)
                                                                                               aGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaat                                                  <  2:1595119/149‑1 (MQ=255)
                                                                                               aGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaat                                                  >  2:186164/1‑149 (MQ=255)
                                                                                               aGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaat                                                  <  1:1784919/149‑1 (MQ=255)
                                                                                                 ccGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaattt                                                <  1:282942/149‑1 (MQ=255)
                                                                                                    tATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTAtt                                             >  2:233027/1‑149 (MQ=255)
                                                                                                    tATGACATTTGATAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTAAATAATTTGTAACCACAAAATATTTGTTATGTTGCAAAAATAACACATTTAATTTAtt                                             >  2:232889/1‑149 (MQ=255)
                                                                                                     aTGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTg                                            >  2:519650/1‑149 (MQ=255)
                                                                                                       gACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGAt                                          <  1:3422733/149‑1 (MQ=255)
                                                                                                       gACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGAt                                          <  2:4042631/149‑1 (MQ=255)
                                                                                                       gACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGAt                                          <  2:3796995/149‑1 (MQ=255)
                                                                                                        aCATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGAtt                                         <  1:3131292/149‑1 (MQ=255)
                                                                                                        aCATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGAtt                                         <  2:1058718/149‑1 (MQ=255)
                                                                                                          aTTTGCTACCGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATtat                                       <  2:4713657/149‑1 (MQ=255)
                                                                                                            ttGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATaa                                     <  1:2982170/149‑1 (MQ=255)
                                                                                                             tGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATaaa                                    <  2:785367/149‑1 (MQ=255)
                                                                                                             tGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATaaa                                    <  1:4410634/149‑1 (MQ=255)
                                                                                                                 aaCGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACACAATATGTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGc                                >  1:4688304/1‑149 (MQ=255)
                                                                                                                  aCGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCt                               <  1:1986717/149‑1 (MQ=255)
                                                                                                                     gcgcGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATGTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTa                            >  2:1803835/1‑149 (MQ=255)
                                                                                                                         ggTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAttt                        <  2:961765/149‑1 (MQ=255)
                                                                                                                           tAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAttttt                      <  2:2728065/149‑1 (MQ=255)
                                                                                                                            aaGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTg                     >  1:1444904/1‑149 (MQ=255)
                                                                                                                              gATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGc                   <  2:1565636/149‑1 (MQ=255)
                                                                                                                               aTCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGcc                  >  1:2778893/1‑149 (MQ=255)
                                                                                                                               aTCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGcc                  >  2:887416/1‑149 (MQ=255)
                                                                                                                                  ggCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGGCCtt               >  1:3077090/1‑149 (MQ=255)
                                                                                                                                   gCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCttt              <  2:3666696/149‑1 (MQ=255)
                                                                                                                                   gCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCttt              <  2:2779214/149‑1 (MQ=255)
                                                                                                                                   gCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCttt              >  1:4011589/1‑149 (MQ=255)
                                                                                                                                   gCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCttt              >  1:1623676/1‑149 (MQ=255)
                                                                                                                                     tttGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTa            <  1:718215/149‑1 (MQ=255)
                                                                                                                                        gtgGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTAttt         <  2:257662/149‑1 (MQ=255)
                                                                                                                                        gtgGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTAttt         <  1:1860505/149‑1 (MQ=255)
                                                                                                                                             tGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGgt    >  2:4519001/1‑149 (MQ=255)
                                                                                                                                               aGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGgtgt  >  2:1497259/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTTTTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGGTGT  >  CP009273/28993‑29284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑