Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 263,270 C→A 10.1% V149V (GTG→GTT)  yafZ ← CP4‑6 prophage; conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273263,2700CA10.1% 1073.0 / 55.4 304V149V (GTG→GTT) yafZCP4‑6 prophage; conserved protein
Reads supporting (aligned to +/- strand):  ref base C (137/135);  new base A (17/15);  total (154/150)
Fisher's exact test for biased strand distribution p-value = 8.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

CCGGCTGGTGGTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATTT  >  CP009273/263122‑263418
                                                                                                                                                    |                                                                                                                                                    
ccGGCTGGTGGTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATc                                                                                                                                                      <  2:4365362/149‑1 (MQ=255)
ccGGCTGGTGGTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATc                                                                                                                                                      >  2:3969963/1‑149 (MQ=255)
ccGGCTGGTGGTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATc                                                                                                                                                      >  1:3864356/1‑149 (MQ=255)
  ggCTGGTGGTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCTACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCAc                                                                                                                                                    <  2:3578522/149‑1 (MQ=255)
   gCTGGTGGTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCAcc                                                                                                                                                   <  2:3094799/149‑1 (MQ=255)
    cTGGTGGTCTTAACCAAAGGGGTATGTGTGGGGGGGTTTTGCCTGGGCGTGCTGTGCGGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCt                                                                                                                                                  <  1:925092/149‑1 (MQ=25)
     tggtggTCTTCACCAAAGGGGTATGTGAGGGCGGCTTTTGCCTGTGCGTGCTGTGCCGGGGGTGGCTACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTg                                                                                                                                                 <  2:3910020/149‑1 (MQ=25)
     tggtggTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTg                                                                                                                                                 >  2:787695/1‑149 (MQ=255)
            cTTCACCAACGGGGTTTGTGAGGGCGGCTTTTGCCTGTGCCTGGTGTGCGGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTcacc                                                                                                                                          <  1:3482654/149‑1 (MQ=25)
            cTTCACCAAAGCGGTTGGGGAGGGCGGCTTTTGCCCGTGCCTGCTGTGCGGGGGGTGGAAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTcacc                                                                                                                                          <  1:1093923/149‑1 (MQ=25)
             ttCACCAAAGCGGTTTGTGAGGGGGGCTTTTGCCTGTGCCTGGTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTcacca                                                                                                                                         <  2:1779478/149‑1 (MQ=255)
                 ccAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACAACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTc                                                                                                                                     >  2:4389688/1‑149 (MQ=255)
                   aaaGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTccc                                                                                                                                   >  2:3827054/1‑149 (MQ=255)
                   aaaGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTccc                                                                                                                                   >  2:2433892/1‑149 (MQ=255)
                     cgCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGAAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTccccc                                                                                                                                 <  1:2755259/148‑1 (MQ=255)
                     aGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTccccc                                                                                                                                 <  1:362721/149‑1 (MQ=255)
                      gCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCt                                                                                                                                >  1:3455065/1‑149 (MQ=255)
                        ggTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTtg                                                                                                                              <  2:1915184/149‑1 (MQ=255)
                         gTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTtgt                                                                                                                             >  1:1607851/1‑149 (MQ=255)
                         gTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTtgt                                                                                                                             >  2:4107745/1‑149 (MQ=255)
                         gTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTtgt                                                                                                                             >  2:3786633/1‑149 (MQ=255)
                          tATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTtgtg                                                                                                                            >  1:4348164/1‑149 (MQ=255)
                           aTGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTtgtgt                                                                                                                           >  1:1001988/1‑149 (MQ=255)
                            tgtgAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTtgtgtg                                                                                                                          >  1:862780/1‑149 (MQ=255)
                              tgAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGc                                                                                                                        <  2:1673380/149‑1 (MQ=255)
                              tgAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGc                                                                                                                        >  2:639214/1‑149 (MQ=255)
                              tgAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTGGTGTGGc                                                                                                                        >  2:2417635/1‑149 (MQ=255)
                                  ggcggcTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCAccc                                                                                                                    <  2:3425011/149‑1 (MQ=255)
                                   gcggcTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCg                                                                                                                   >  1:3774880/1‑149 (MQ=255)
                                      gcTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCAc                                                                                                                >  1:1459000/1‑149 (MQ=255)
                                       cTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCAcc                                                                                                               >  2:2940775/1‑149 (MQ=255)
                                        ttttGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCt                                                                                                              <  1:1161195/149‑1 (MQ=255)
                                        ttttGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCt                                                                                                              >  1:1485540/1‑149 (MQ=255)
                                        ttttGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCt                                                                                                              >  2:3876947/1‑149 (MQ=255)
                                         tttGCCTGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTc                                                                                                             <  1:469919/149‑1 (MQ=255)
                                          ttGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCg                                                                                                            >  2:4231585/1‑149 (MQ=255)
                                                gggCCTGCTGGGCCGGGGGTGGAAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAAc                                                                                                      <  1:2538565/147‑1 (MQ=255)
                                                  gCCTGGTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGa                                                                                                    <  1:3484834/149‑1 (MQ=255)
                                                    ctgctgTGCGGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACt                                                                                                  <  2:2624132/149‑1 (MQ=255)
                                                              gggggTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGAc                                                                                        >  1:2653799/1‑149 (MQ=255)
                                                                  gTGGCAACCGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGc                                                                                    <  2:4425121/149‑1 (MQ=255)
                                                                  gTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGc                                                                                    <  2:3321459/149‑1 (MQ=255)
                                                                    ggCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGccc                                                                                  >  2:3387349/1‑149 (MQ=255)
                                                                    ggCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCACCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTGGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGccc                                                                                  >  1:2338771/1‑149 (MQ=255)
                                                                     gCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCg                                                                                 >  1:3734948/1‑149 (MQ=255)
                                                                       aaCAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGtt                                                                               >  2:3355921/1‑149 (MQ=255)
                                                                       aaCAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGtt                                                                               >  2:3729646/1‑149 (MQ=255)
                                                                        aCAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTc                                                                              <  2:3059364/149‑1 (MQ=255)
                                                                         cAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTGGTGTGGCACCCGCACTTCGCCAAACGACTCACCGCAGACGAGCCCGTTCt                                                                             <  2:4053586/149‑1 (MQ=255)
                                                                          aGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTg                                                                            <  1:4553944/149‑1 (MQ=255)
                                                                            cAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGAc                                                                          <  2:4482181/149‑1 (MQ=255)
                                                                             aaCGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACa                                                                         >  2:3241220/1‑149 (MQ=255)
                                                                              aCGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACaa                                                                        >  1:2444276/1‑149 (MQ=255)
                                                                                gACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAAc                                                                      >  1:335085/1‑149 (MQ=255)
                                                                                   tGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACACACCGc                                                                   >  1:2179699/1‑149 (MQ=255)
                                                                                       tGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGa                                                               <  2:2111791/149‑1 (MQ=255)
                                                                                        ggCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGaa                                                              <  1:505643/149‑1 (MQ=255)
                                                                                        ggCATCCCGTTTCTCCTCCACCCGGTAAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGaa                                                              <  1:2898317/149‑1 (MQ=255)
                                                                                         gCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGCGCCCGTTCTGACAAACCGCACGaaa                                                             >  1:2984107/1‑149 (MQ=255)
                                                                                            tCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAg                                                          >  2:2020444/1‑149 (MQ=255)
                                                                                                gTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTccc                                                      <  1:4065085/149‑1 (MQ=255)
                                                                                                 tttCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCg                                                     >  1:2116567/1‑149 (MQ=255)
                                                                                                     tcctccACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCaa                                                 <  2:4103478/149‑1 (MQ=255)
                                                                                                      cctccACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAAc                                                <  1:127686/149‑1 (MQ=255)
                                                                                                          cACCGGGTCAAAAATCCCCAGCACCTCATACGCGCATTCAATCACCTGACTCACCACTTCCCCCTTTTGTGGCACCCGCACCTCGCCACACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCt                                            <  1:3224932/149‑1 (MQ=255)
                                                                                                            cccTGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCTCGTCCCCCTTGTGTGGCACCCGAACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGa                                          <  2:2197279/149‑1 (MQ=255)
                                                                                                            cccGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGa                                          >  1:1522251/1‑149 (MQ=255)
                                                                                                             ccGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGAt                                         >  2:4738404/1‑149 (MQ=255)
                                                                                                              cGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATa                                        <  2:2767678/149‑1 (MQ=255)
                                                                                                              cGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATa                                        <  2:2814846/149‑1 (MQ=255)
                                                                                                                   aaaaaTCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAAc                                   <  2:1271001/149‑1 (MQ=255)
                                                                                                                    aaaaTCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTCGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACt                                  >  2:154777/1‑149 (MQ=255)
                                                                                                                     aaaTCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTg                                 >  1:246808/1‑149 (MQ=255)
                                                                                                                          cccAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTcc                            <  2:2146451/149‑1 (MQ=255)
                                                                                                                           ccAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCa                           >  2:4649391/1‑149 (MQ=255)
                                                                                                                           ccAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCa                           >  1:2715153/1‑149 (MQ=255)
                                                                                                                            cAGCACCTCATACGCGCCTTCAATAACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCAt                          <  1:4780332/149‑1 (MQ=255)
                                                                                                                             aGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATc                         <  2:1079436/149‑1 (MQ=255)
                                                                                                                               cACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGt                       <  1:4284260/149‑1 (MQ=255)
                                                                                                                                 ccTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTga                     <  2:4618113/149‑1 (MQ=255)
                                                                                                                                 ccTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTga                     >  1:3174435/1‑149 (MQ=255)
                                                                                                                                  cTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTgag                    <  2:3615614/149‑1 (MQ=255)
                                                                                                                                   tCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTgaga                   <  2:3455314/149‑1 (MQ=255)
                                                                                                                                     aTACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGt                 <  2:2243103/149‑1 (MQ=255)
                                                                                                                                        cgcgCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCTTGAGAGTTGa              <  2:291655/149‑1 (MQ=255)
                                                                                                                                        cgcgCCTTCAATCACCTGACTCACCACGTCCCCCTGGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGa              <  1:570986/149‑1 (MQ=255)
                                                                                                                                         gcgcCTTCAATAACCTGCCTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAg             <  1:179447/149‑1 (MQ=255)
                                                                                                                                          cgcCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGt            <  2:3864356/149‑1 (MQ=255)
                                                                                                                                          cgcCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGt            >  2:4173493/1‑149 (MQ=255)
                                                                                                                                          cgcCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGt            >  2:1392664/1‑149 (MQ=255)
                                                                                                                                           gcCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACAACTGATACGAACTGGTTCCATCGTGAGAGTTGAGTa           >  1:2603134/1‑149 (MQ=255)
                                                                                                                                            ccTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAg          >  1:1854841/1‑149 (MQ=255)
                                                                                                                                              ttCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGaa        <  1:4228055/149‑1 (MQ=255)
                                                                                                                                              ttCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGaa        <  1:1415210/149‑1 (MQ=255)
                                                                                                                                                cAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGaata      >  2:4568725/1‑149 (MQ=255)
                                                                                                                                                 aaTCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCACCATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGaataa     >  1:131907/1‑149 (MQ=255)
                                                                                                                                                 aaTCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGaataa     <  1:6106/149‑1 (MQ=255)
                                                                                                                                                  aTCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGaataat    <  1:3969963/149‑1 (MQ=255)
                                                                                                                                                  aTCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGaataat    <  2:3255143/149‑1 (MQ=255)
                                                                                                                                                   tCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAAtt   <  1:4138553/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGGCTGGTGGTCTTCACCAAAGCGGTATGTGAGGGCGGCTTTTGCCAGTGCCTGCTGTGCCGGGGGTGGCAACAGCAACGACTGCATGGCATCCCGTTTCTCCTCCACCCGGTCAAAAATCCCCAGCACCTCATACGCGCCTTCAATCACCTGACTCACCACGTCCCCCTTGTGTGGCACCCGCACCTCGCCAAACGACTCACCGCAGACGAGCCCGTTCTGACAAACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATTT  >  CP009273/263122‑263418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑