Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,365,189 C→A 7.7% A249S (GCG→TCG)  nanT ← sialic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,365,1890CA 7.7% 1021.8 / 37.4 273A249S (GCG→TCG) nanTsialic acid transporter
Reads supporting (aligned to +/- strand):  ref base C (123/129);  new base A (17/4);  total (140/133)
Fisher's exact test for biased strand distribution p-value = 5.55e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 282 total aligned reads displayed.

AGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGTTTCTCTTTCCAG  >  CP009273/3365041‑3365337
                                                                                                                                                    |                                                                                                                                                    
 gcagcGCCTGAATCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATcgcg                                                                                                                                                     <  2:3937111/149‑1 (MQ=255)
  cagcGCCTGAATCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCgg                                                                                                                                                    >  1:1838106/1‑149 (MQ=255)
    gcgcCTGAATCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCa                                                                                                                                                  <  1:1133814/149‑1 (MQ=255)
     cgcCTGAATCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCAt                                                                                                                                                 <  1:196360/149‑1 (MQ=255)
      gcCTGAATCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCAtt                                                                                                                                                <  2:670852/149‑1 (MQ=255)
      gcCTGAATCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCAtt                                                                                                                                                >  2:4161510/1‑149 (MQ=255)
        cTGAATCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCAtttt                                                                                                                                              >  2:2491621/1‑149 (MQ=255)
         tGAATCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTg                                                                                                                                             >  2:817711/1‑149 (MQ=255)
          gAATCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGc                                                                                                                                            <  2:4225277/149‑1 (MQ=255)
            aTCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAg                                                                                                                                          >  1:262244/1‑149 (MQ=255)
             tCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGAGGCATTTTGCAgg                                                                                                                                         >  2:3281229/1‑149 (MQ=255)
              cGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGt                                                                                                                                        <  1:4727395/149‑1 (MQ=255)
               ggCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGtt                                                                                                                                       >  2:2439838/1‑149 (MQ=255)
               ggCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGtt                                                                                                                                       <  1:4579512/149‑1 (MQ=255)
               ggCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGAGGCATTTTGCAGGtt                                                                                                                                       >  1:1250059/1‑149 (MQ=255)
                gCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTa                                                                                                                                      >  1:4307102/1‑149 (MQ=255)
                  cATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTAcc                                                                                                                                    <  2:3106204/149‑1 (MQ=255)
                  cATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTAcc                                                                                                                                    >  1:3206450/1‑149 (MQ=255)
                   aTGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCg                                                                                                                                   <  1:1811916/149‑1 (MQ=255)
                   aTGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCg                                                                                                                                   <  1:677451/149‑1 (MQ=255)
                    tGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCgg                                                                                                                                  >  2:3521064/1‑149 (MQ=255)
                    tGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCgg                                                                                                                                  >  1:596953/1‑149 (MQ=255)
                     gAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGc                                                                                                                                 >  1:3677806/1‑149 (MQ=255)
                     gAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGAGGCATTTTGCAGGTTACCGGc                                                                                                                                 >  2:2327768/1‑149 (MQ=255)
                          gagGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGc                                                                                                                            <  2:156964/149‑1 (MQ=255)
                              aaaGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGTGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGaa                                                                                                                        >  1:4335743/1‑149 (MQ=255)
                                aGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAAcc                                                                                                                      >  1:3906117/1‑149 (MQ=255)
                                 gCAAACAACCCGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACca                                                                                                                     <  2:3971232/149‑1 (MQ=255)
                                 gCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACca                                                                                                                     <  2:2562701/149‑1 (MQ=255)
                                   aaaCAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACcaca                                                                                                                   <  1:1071940/149‑1 (MQ=255)
                                       aacaCGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAgcgc                                                                                                               >  2:1779240/1‑149 (MQ=255)
                                       aacaCGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAgcgc                                                                                                               <  2:4268035/149‑1 (MQ=255)
                                        acacGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATAGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCa                                                                                                              >  1:3071794/1‑149 (MQ=255)
                                          acGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGt                                                                                                            >  2:3803244/1‑149 (MQ=255)
                                             accaccATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAgc                                                                                                         >  1:1553160/1‑149 (MQ=255)
                                              ccaccaTCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAgcc                                                                                                        >  1:2171092/1‑149 (MQ=255)
                                              ccaccaTCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAgcc                                                                                                        <  1:954603/149‑1 (MQ=255)
                                                  caTCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAgccgccg                                                                                                    >  1:1121596/1‑149 (MQ=255)
                                                    tCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAgccgccgcc                                                                                                  <  2:2805444/149‑1 (MQ=255)
                                                    tCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAgccgccgcc                                                                                                  >  1:773023/1‑149 (MQ=255)
                                                    tCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGAGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAgccgccgcc                                                                                                  >  1:4505324/1‑149 (MQ=255)
                                                          ttACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGAGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTc                                                                                            >  2:210040/1‑149 (MQ=255)
                                                             cGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCAtt                                                                                         <  2:3241311/149‑1 (MQ=255)
                                                             cGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCAtt                                                                                         <  2:2925479/149‑1 (MQ=255)
                                                              gCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTa                                                                                        >  1:3287715/1‑149 (MQ=255)
                                                                          gCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCaa                                                                            >  1:1716665/1‑149 (MQ=255)
                                                                          gCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCaa                                                                            >  1:3045875/1‑149 (MQ=255)
                                                                             tttGCCTGCACTCTGCACCATAAAGCTTATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACAGGCGAAGCAGAACCACAGCTCAGTAGCCGCCGAAAGTGTCATTACGATATTGTCAATGc                                                                         <  2:4520741/149‑1 (MQ=255)
                                                                             tttGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGc                                                                         <  2:3890846/149‑1 (MQ=255)
                                                                               tGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGa                                                                       <  2:447637/149‑1 (MQ=255)
                                                                                gCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACCGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCAGTACGATATTGGCAATGCGAt                                                                      >  2:4071097/1‑149 (MQ=255)
                                                                                gCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGAt                                                                      >  2:4369117/1‑149 (MQ=255)
                                                                                gCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGAt                                                                      >  2:332688/1‑149 (MQ=255)
                                                                                   tGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGtt                                                                   >  1:3028524/1‑149 (MQ=255)
                                                                                    gCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTc                                                                  >  2:4684365/1‑149 (MQ=255)
                                                                                      aCTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTcac                                                                <  2:1022367/149‑1 (MQ=255)
                                                                                      aCTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTcac                                                                >  1:4524609/1‑149 (MQ=255)
                                                                                      aCTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTcac                                                                >  1:1699225/1‑149 (MQ=255)
                                                                                             aCCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTa                                                         >  1:4284621/1‑149 (MQ=255)
                                                                                                ataccgCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGAGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTagag                                                      <  2:1009281/144‑1 (MQ=255)
                                                                                                 tAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACTATATTGGCAATGCGATGTTCACCACGGTAgaga                                                     <  1:3955939/149‑1 (MQ=255)
                                                                                                     gCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAatat                                                 >  1:3790221/1‑149 (MQ=255)
                                                                                                        gATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATcca                                              >  1:2279188/1‑149 (MQ=255)
                                                                                                        gATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATcca                                              <  2:2767897/149‑1 (MQ=255)
                                                                                                        gATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATcca                                              <  2:2657829/149‑1 (MQ=255)
                                                                                                         aTAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATccac                                             >  2:1975027/1‑149 (MQ=255)
                                                                                                            aaGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATccaccct                                          >  2:3443109/1‑147 (MQ=255)
                                                                                                            aaGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCAt                                          <  1:1011836/149‑1 (MQ=255)
                                                                                                            aaGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGAGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCAt                                          >  1:1912331/1‑149 (MQ=255)
                                                                                                             aGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCAtt                                         >  1:4091278/1‑149 (MQ=255)
                                                                                                                ttGCGGCGCATAACAGCCCAAGAACAGCGTCGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATtgtg                                      >  2:1259148/1‑149 (MQ=255)
                                                                                                                ttGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATtgtg                                      >  1:1470921/1‑149 (MQ=255)
                                                                                                                  gcggcgCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCg                                    >  2:4553831/1‑149 (MQ=255)
                                                                                                                   cggcgCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGt                                   <  1:1342709/149‑1 (MQ=255)
                                                                                                                    ggcgCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACTATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTa                                  <  1:3827499/149‑1 (MQ=255)
                                                                                                                    ggcgCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTa                                  <  1:1759585/149‑1 (MQ=255)
                                                                                                                    ggcgCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCACTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTa                                  <  1:2402736/149‑1 (MQ=255)
                                                                                                                     gcgcATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTAc                                 >  1:1227044/1‑149 (MQ=255)
                                                                                                                         aTAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGt                             <  2:4022797/149‑1 (MQ=255)
                                                                                                                          tAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTg                            >  2:3039399/1‑149 (MQ=255)
                                                                                                                           aaCAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGc                           <  1:3439329/149‑1 (MQ=255)
                                                                                                                            aCAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCt                          <  2:4449342/149‑1 (MQ=255)
                                                                                                                            aCAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCt                          <  2:2088107/149‑1 (MQ=255)
                                                                                                                            aCAGCCCAAGAACAGCGACGATCGAGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCt                          >  1:2725475/1‑149 (MQ=255)
                                                                                                                               gCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACTATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTa                       <  2:1838106/149‑1 (MQ=255)
                                                                                                                               gCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTa                       >  2:1739406/1‑149 (MQ=255)
                                                                                                                                cccAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTAc                      <  2:2896666/149‑1 (MQ=255)
                                                                                                                                      aaCCGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCgt                <  1:93675/149‑1 (MQ=255)
                                                                                                                                        cAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCgtgt              <  2:2152597/149‑1 (MQ=255)
                                                                                                                                         aGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGtt             <  2:2967165/149‑1 (MQ=255)
                                                                                                                                          gCGACGATCGAGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGttt            >  1:1453523/1‑149 (MQ=255)
                                                                                                                                          gCGACGATCGAGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGttt            >  2:1469443/1‑149 (MQ=255)
                                                                                                                                           cgacgaTCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGTTtc           >  2:3870461/1‑149 (MQ=255)
                                                                                                                                           cgacgaTCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGTTtc           <  2:3641335/149‑1 (MQ=255)
                                                                                                                                            gacgaTCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGTTtct          <  1:3497519/149‑1 (MQ=255)
                                                                                                                                            gacgaTCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGTTtct          <  2:569154/149‑1 (MQ=255)
                                                                                                                                              cgaTCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGTTTctct        <  2:2171038/149‑1 (MQ=255)
                                                                                                                                                   gcgGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGTTTCTCTTTCCa   <  1:4049147/149‑1 (MQ=255)
                                                                                                                                                   gcgGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGTTTCTCTTTCCa   <  1:4171141/149‑1 (MQ=255)
                                                                                                                                                   gcgGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGTTTCTCTTTCCa   <  1:3407196/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGCAGCGCCTGAATCGGCCATGAGTAGAGGAAAGCAAACAACACGACCACCATCAGCATTACGCCCGTTGGCCAGCGTTTGCCTGCACTCTGCACCATAAAGCTGATAAAGATTGCGGCGCATAACAGCCCAAGAACAGCGACGATCGCGGCATTTTGCAGGTTACCGGCGAAGCAGAACCACAGCGCAGTAGCCGCCGCCAGTGTCATTACGATATTGGCAATGCGATGTTCACCACGGTAGAGAATATCCACCATTGTGCGTACTGGTGCTTTACCTGCGTGTTTCTCTTTCCAG  >  CP009273/3365041‑3365337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑