Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 279,800 G→T 91.3% E297* (GAA→TAA)  yagF → CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273279,8000GT91.3% 697.3 / 60.4 253E297* (GAA→TAA) yagFCP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base G (10/12);  new base T (120/111);  total (130/123)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

Only 100 of 254 total aligned reads displayed.

CTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGT  >  CP009273/279656‑279948
                                                                                                                                                |                                                                                                                                                    
cTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTaaaa                                                                                                                                                  <  2:3169877/149‑1 (MQ=255)
 tGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAAc                                                                                                                                                 <  1:4470615/149‑1 (MQ=255)
   ggTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAAcgc                                                                                                                                               <  2:3811338/149‑1 (MQ=255)
    gTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCGGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAAcgcg                                                                                                                                              >  1:2831366/1‑149 (MQ=255)
        ggCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATgg                                                                                                                                          <  2:2500040/149‑1 (MQ=255)
        ggCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATgg                                                                                                                                          <  2:3766129/149‑1 (MQ=255)
        ggCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATgg                                                                                                                                          <  1:1828352/149‑1 (MQ=255)
          cgcTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTg                                                                                                                                        <  2:951128/149‑1 (MQ=255)
          cgcTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTg                                                                                                                                        <  2:2601804/149‑1 (MQ=255)
          cgcTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTg                                                                                                                                        <  2:557611/149‑1 (MQ=255)
               ccGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCa                                                                                                                                   >  1:577244/1‑149 (MQ=255)
                  cACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCAcgc                                                                                                                                >  1:1426600/1‑149 (MQ=255)
                  cACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCAcgc                                                                                                                                <  1:4096020/149‑1 (MQ=255)
                  cACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCAcgc                                                                                                                                <  1:2713927/149‑1 (MQ=255)
                   aCTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCAcgcg                                                                                                                               >  1:2435243/1‑149 (MQ=255)
                   aCTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCAcgcg                                                                                                                               >  1:2562143/1‑149 (MQ=255)
                     tCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcggc                                                                                                                             >  1:835088/1‑149 (MQ=255)
                     tCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcggc                                                                                                                             >  1:344056/1‑149 (MQ=255)
                       cgcgCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGt                                                                                                                           <  2:3715575/149‑1 (MQ=255)
                       cgcgCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGt                                                                                                                           <  2:1492076/149‑1 (MQ=255)
                        gcgcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGtt                                                                                                                          <  1:1334896/149‑1 (MQ=255)
                               cgcCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCggcggc                                                                                                                   <  2:4653266/149‑1 (MQ=255)
                               cgcCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCggcggc                                                                                                                   <  1:496121/149‑1 (MQ=255)
                                  cGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTcc                                                                                                                <  2:2127336/149‑1 (MQ=255)
                                   gTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTcca                                                                                                               >  1:1713665/1‑149 (MQ=255)
                                    tCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTccac                                                                                                              >  1:208106/1‑149 (MQ=255)
                                      cGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTccacca                                                                                                            <  2:4214578/149‑1 (MQ=255)
                                       gggCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCaa                                                                                                           <  2:242137/149‑1 (MQ=255)
                                         gCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAAtt                                                                                                         <  2:3592000/149‑1 (MQ=255)
                                         gCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAAtt                                                                                                         <  2:4113319/149‑1 (MQ=255)
                                          cAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAAttt                                                                                                        <  1:2499771/149‑1 (MQ=255)
                                            ggCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTAc                                                                                                      >  1:4234289/1‑149 (MQ=255)
                                            ggCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTAc                                                                                                      >  1:4234273/1‑149 (MQ=255)
                                            ggCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTAc                                                                                                      >  1:3083798/1‑149 (MQ=255)
                                                 tgtgGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTActgctg                                                                                                 >  1:187173/1‑149 (MQ=255)
                                                 tgtgGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTActgctg                                                                                                 >  1:4704913/1‑149 (MQ=255)
                                                  gtgGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACtgctgc                                                                                                >  1:1037872/1‑149 (MQ=255)
                                                    ggCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGcac                                                                                              <  2:81873/149‑1 (MQ=255)
                                                    ggCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGcac                                                                                              >  1:3376570/1‑149 (MQ=255)
                                                     gCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGcaca                                                                                             <  2:1147328/149‑1 (MQ=255)
                                                      cTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACAt                                                                                            <  1:395417/149‑1 (MQ=255)
                                                      cTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACAt                                                                                            >  2:897347/1‑149 (MQ=255)
                                                         gagaTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTcc                                                                                         <  1:195303/149‑1 (MQ=255)
                                                             tCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGcc                                                                                     <  2:4715724/149‑1 (MQ=255)
                                                                cccgccAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATc                                                                                  <  2:1267727/149‑1 (MQ=255)
                                                                    ccAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGccc                                                                              <  1:3526073/149‑1 (MQ=255)
                                                                     cAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCa                                                                             >  2:4585338/1‑149 (MQ=255)
                                                                     cAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCa                                                                             >  1:322383/1‑149 (MQ=255)
                                                                       gTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCAcg                                                                           >  1:3049106/1‑149 (MQ=255)
                                                                           gcgcgcgcGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACgcggc                                                                       <  1:697296/149‑1 (MQ=255)
                                                                             gcgcgcgGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGcggcgg                                                                     >  1:2202598/1‑149 (MQ=255)
                                                                               gcgcgGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCCAGTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGc                                                                   >  1:2927259/1‑149 (MQ=255)
                                                                                  cgGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGc                                                                <  2:3974563/149‑1 (MQ=255)
                                                                                    gTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCAc                                                              >  2:3833900/1‑149 (MQ=255)
                                                                                    gTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCAc                                                              >  2:22803/1‑149 (MQ=255)
                                                                                       aGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGAt                                                           >  1:4415132/1‑149 (MQ=255)
                                                                                        gCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATc                                                          <  2:309292/149‑1 (MQ=255)
                                                                                        gCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATc                                                          >  1:160912/1‑149 (MQ=255)
                                                                                         cGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCTATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATcc                                                         <  1:453506/149‑1 (MQ=255)
                                                                                         cGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATcc                                                         >  2:1698822/1‑149 (MQ=255)
                                                                                          gAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATccc                                                        <  2:3518042/149‑1 (MQ=255)
                                                                                          gAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATccc                                                        <  1:4144702/149‑1 (MQ=255)
                                                                                          gAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATccc                                                        <  1:3522486/149‑1 (MQ=255)
                                                                                          gAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCAGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATccc                                                        >  1:2896212/1‑149 (MQ=255)
                                                                                            gCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCgg                                                      >  2:3637132/1‑149 (MQ=255)
                                                                                            gCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCgg                                                      >  2:1854073/1‑149 (MQ=255)
                                                                                             cTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGa                                                     <  1:1164572/149‑1 (MQ=255)
                                                                                                gATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGt                                                  >  1:3841086/1‑149 (MQ=255)
                                                                                                gATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCAAGATCCCGGACGt                                                  <  1:1486158/149‑1 (MQ=255)
                                                                                                 aTAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGtt                                                 <  1:1697452/149‑1 (MQ=255)
                                                                                                 aTAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGtt                                                 <  2:837385/149‑1 (MQ=255)
                                                                                                  tAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTg                                                >  2:4140013/1‑149 (MQ=255)
                                                                                                   aGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGa                                               >  1:1754041/1‑149 (MQ=255)
                                                                                                   aGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGa                                               <  1:3639255/149‑1 (MQ=255)
                                                                                                    gCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAg                                              >  1:2647183/1‑149 (MQ=255)
                                                                                                    gCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAg                                              >  1:377510/1‑149 (MQ=255)
                                                                                                     ccGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGc                                             <  2:4035560/149‑1 (MQ=255)
                                                                                                          gCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTgg                                        >  1:4106945/1‑149 (MQ=255)
                                                                                                            aTCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGAc                                      >  1:3181911/1‑149 (MQ=255)
                                                                                                            aTCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGAc                                      >  1:4065039/1‑149 (MQ=255)
                                                                                                                       ggATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAcc                           <  1:4621007/149‑1 (MQ=255)
                                                                                                                        gATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCg                          >  2:2901617/1‑149 (MQ=255)
                                                                                                                           atCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTaa                       <  2:489930/149‑1 (MQ=255)
                                                                                                                            tCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTaaa                      >  2:2881109/1‑149 (MQ=255)
                                                                                                                               tctcCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTACGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTg                   >  2:4395792/1‑149 (MQ=255)
                                                                                                                                ctcCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGc                  <  1:1625475/149‑1 (MQ=255)
                                                                                                                                   cGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCcgc               >  2:2238496/1‑149 (MQ=255)
                                                                                                                                   cGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCcgc               >  2:2238905/1‑149 (MQ=255)
                                                                                                                                    gATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCcgcg              >  2:1440565/1‑149 (MQ=255)
                                                                                                                                     aTAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGt             <  2:1037872/149‑1 (MQ=255)
                                                                                                                                       aaaGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCt           <  2:89057/149‑1 (MQ=255)
                                                                                                                                       aaaGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGAACATTCCGGCCATCGCCCACTCGGCGGGCTGCACGATACCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCt           >  1:1374918/1‑149 (MQ=255)
                                                                                                                                        aaGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTg          >  1:4159805/1‑149 (MQ=255)
                                                                                                                                          gCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGt        >  2:3059449/1‑149 (MQ=255)
                                                                                                                                            cATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGa      >  2:1195691/1‑149 (MQ=255)
                                                                                                                                            cATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGa      <  1:231701/149‑1 (MQ=255)
                                                                                                                                            cATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGa      <  2:3454219/149‑1 (MQ=255)
                                                                                                                                            cATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGa      <  1:2623922/149‑1 (MQ=255)
                                                                                                                                             aTCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAg     >  1:94365/1‑149 (MQ=255)
                                                                                                                                                gAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGt  >  2:1578352/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGT  >  CP009273/279656‑279948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑