Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,473,139 G→T 100% I276I (ATC→ATA)  kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,473,1390GT99.6% 834.8 / ‑4.1 251I276I (ATC→ATA) kefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (104/146);  total (105/146)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 257 total aligned reads displayed.

TGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGG  >  CP009273/3472993‑3473284
                                                                                                                                                  |                                                                                                                                                 
tGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGatat                                                                                                                                                 >  2:866861/1‑149 (MQ=255)
 gCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAtata                                                                                                                                                >  1:911678/1‑149 (MQ=255)
   aTCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATaaa                                                                                                                                              >  2:4373007/1‑149 (MQ=255)
     ccGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGa                                                                                                                                            <  1:1187446/149‑1 (MQ=255)
      cGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGaa                                                                                                                                           <  2:1370286/149‑1 (MQ=255)
       gCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAAc                                                                                                                                          <  2:148535/149‑1 (MQ=255)
       gCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAAc                                                                                                                                          >  2:1909742/1‑149 (MQ=255)
        ctctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACa                                                                                                                                         >  1:1944333/1‑149 (MQ=255)
         tctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACaa                                                                                                                                        >  1:333267/1‑149 (MQ=255)
          ctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACaaa                                                                                                                                       <  2:707727/149‑1 (MQ=255)
           tGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAAc                                                                                                                                      >  1:316657/1‑149 (MQ=255)
           tGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAAc                                                                                                                                      <  1:2118573/149‑1 (MQ=255)
               cTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGag                                                                                                                                  <  1:3767811/149‑1 (MQ=255)
                 acgcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGagca                                                                                                                                <  2:3846579/149‑1 (MQ=255)
                  cgcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGagcag                                                                                                                               >  1:1007176/1‑149 (MQ=255)
                     acgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCaa                                                                                                                            <  2:3250593/149‑1 (MQ=255)
                        ccATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGcc                                                                                                                         <  2:430208/149‑1 (MQ=255)
                          aTACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCtt                                                                                                                       <  1:4631981/149‑1 (MQ=255)
                           tACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCttt                                                                                                                      <  1:3056137/149‑1 (MQ=255)
                            aCAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTg                                                                                                                     <  2:824286/149‑1 (MQ=255)
                               aTCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAg                                                                                                                  <  2:2943985/149‑1 (MQ=255)
                                tCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACACCAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAgg                                                                                                                 <  2:1763629/149‑1 (MQ=255)
                                tCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAgg                                                                                                                 <  2:1250439/149‑1 (MQ=255)
                                 cgcgCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAggg                                                                                                                >  2:4683307/1‑149 (MQ=255)
                                  gcgcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGa                                                                                                               <  1:1339531/149‑1 (MQ=255)
                                   cgcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGAt                                                                                                              <  1:480081/149‑1 (MQ=255)
                                       agcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGAt                                                                                                          >  1:1659903/1‑149 (MQ=255)
                                       agcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGAt                                                                                                          >  1:3593752/1‑149 (MQ=255)
                                              aCAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGtt                                                                                                   <  2:575966/149‑1 (MQ=255)
                                               cAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGttt                                                                                                  <  2:1419971/149‑1 (MQ=255)
                                                aGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTc                                                                                                 <  2:911678/149‑1 (MQ=255)
                                                 gCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTcc                                                                                                >  2:2231941/1‑149 (MQ=255)
                                                  cACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCa                                                                                               >  2:2337086/1‑149 (MQ=255)
                                                  cACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCa                                                                                               <  1:3446543/149‑1 (MQ=255)
                                                    cGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGt                                                                                             <  1:1203890/149‑1 (MQ=255)
                                                     gagaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGtt                                                                                            >  2:3933833/1‑149 (MQ=255)
                                                        aaTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCAt                                                                                         <  2:2268721/149‑1 (MQ=255)
                                                        aaTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCAt                                                                                         <  2:2204119/149‑1 (MQ=255)
                                                          ttttCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATgg                                                                                       <  1:1079592/149‑1 (MQ=255)
                                                              cACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGat                                                                                   <  1:1977012/149‑1 (MQ=255)
                                                              cACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGat                                                                                   <  2:3181425/149‑1 (MQ=255)
                                                               aCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGata                                                                                  <  2:1956248/149‑1 (MQ=255)
                                                                   ccaccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCa                                                                              >  2:1993268/1‑149 (MQ=255)
                                                                    caccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGATAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCAc                                                                             <  1:4043123/149‑1 (MQ=255)
                                                                     accaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACt                                                                            >  2:1503887/1‑149 (MQ=255)
                                                                        aGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTc                                                                         <  1:4298557/149‑1 (MQ=255)
                                                                         gCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTcc                                                                        >  2:4342752/1‑149 (MQ=255)
                                                                         gCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTcc                                                                        >  1:2517190/1‑149 (MQ=255)
                                                                            caACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGTCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                     >  2:3785059/1‑149 (MQ=255)
                                                                            caACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGTCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                     >  2:2582557/1‑149 (MQ=255)
                                                                            caACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                     >  2:3112128/1‑149 (MQ=255)
                                                                                cACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcagca                                                                 <  1:1852060/149‑1 (MQ=255)
                                                                                cACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcagca                                                                 <  1:2912068/149‑1 (MQ=255)
                                                                                cACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcagca                                                                 <  1:2912058/149‑1 (MQ=255)
                                                                                cACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcagca                                                                 <  1:4670934/149‑1 (MQ=255)
                                                                                  cGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCagcagc                                                               <  1:183775/149‑1 (MQ=255)
                                                                                     ttATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACg                                                            >  1:3746607/1‑149 (MQ=255)
                                                                                      tATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGc                                                           >  1:1435742/1‑149 (MQ=255)
                                                                                       aTCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGcc                                                          >  2:4242894/1‑149 (MQ=255)
                                                                                        tCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGccc                                                         <  1:2742359/149‑1 (MQ=255)
                                                                                        tCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGccc                                                         >  1:2792998/1‑149 (MQ=255)
                                                                                        tCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGccc                                                         <  1:350465/149‑1 (MQ=255)
                                                                                            tACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCaa                                                     <  2:4188143/149‑1 (MQ=255)
                                                                                            tACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCaa                                                     <  1:3212646/149‑1 (MQ=255)
                                                                                             aCCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAAt                                                    >  1:1278225/1‑149 (MQ=255)
                                                                                               ccACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATaa                                                  >  2:3517169/1‑149 (MQ=255)
                                                                                                cacaACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATaaa                                                 <  1:3369247/149‑1 (MQ=255)
                                                                                                  caacaGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATg                                               <  1:2286346/149‑1 (MQ=255)
                                                                                                   aacaGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGt                                              >  1:3912618/1‑149 (MQ=255)
                                                                                                   aacaGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGt                                              >  2:774550/1‑149 (MQ=255)
                                                                                                   aacaGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGt                                              >  2:4402023/1‑149 (MQ=255)
                                                                                                     caGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCCTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCACTAAATGTAc                                            >  2:1114658/1‑149 (MQ=255)
                                                                                                      aGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTAcc                                           <  1:604936/149‑1 (MQ=255)
                                                                                                       gATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCg                                          >  2:852529/1‑149 (MQ=255)
                                                                                                         tGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAg                                        >  1:4261669/1‑149 (MQ=255)
                                                                                                          gggTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAgc                                       <  2:3063628/149‑1 (MQ=255)
                                                                                                          gggTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAgc                                       <  2:3703642/149‑1 (MQ=255)
                                                                                                              ataAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCa                                   >  2:2491023/1‑149 (MQ=255)
                                                                                                              ataAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCa                                   >  2:3641225/1‑149 (MQ=255)
                                                                                                               taAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCAt                                  >  2:1446076/1‑149 (MQ=255)
                                                                                                               taAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCAt                                  >  2:1446383/1‑149 (MQ=255)
                                                                                                               taAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCAt                                  >  1:471973/1‑149 (MQ=255)
                                                                                                                aaaGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATc                                 <  2:810589/149‑1 (MQ=255)
                                                                                                                 aaGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCg                                >  2:1907515/1‑149 (MQ=255)
                                                                                                                   gCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGAc                              <  1:803922/149‑1 (MQ=255)
                                                                                                                     aCCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAg                            >  1:2460789/1‑149 (MQ=255)
                                                                                                                     aCCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAg                            >  1:2998141/1‑149 (MQ=255)
                                                                                                                         cGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGcccc                        <  1:4378199/149‑1 (MQ=255)
                                                                                                                         cGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGcccc                        <  1:1988641/149‑1 (MQ=255)
                                                                                                                         cGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGcccc                        <  2:299166/149‑1 (MQ=255)
                                                                                                                         cGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGcccc                        <  2:2845805/149‑1 (MQ=255)
                                                                                                                         cGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGcccc                        <  2:2693214/149‑1 (MQ=255)
                                                                                                                                gAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCAt                 >  1:990173/1‑149 (MQ=255)
                                                                                                                                gAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCAt                 <  1:4477917/149‑1 (MQ=255)
                                                                                                                                  gTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATcc               >  1:177338/1‑149 (MQ=255)
                                                                                                                                   tGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCa              <  2:2246927/149‑1 (MQ=255)
                                                                                                                                        tGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAAc         >  1:3686041/1‑149 (MQ=255)
                                                                                                                                           cGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAAt      <  2:3958316/149‑1 (MQ=255)
                                                                                                                                           cGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAAt      >  2:1411131/1‑149 (MQ=255)
                                                                                                                                            gACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATg     >  2:2462867/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGG  >  CP009273/3472993‑3473284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑