Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,504,143 G→T 100% R65S (CGT→AGT)  yhfX ← putative pyridoxal 5'‑phosphate binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,504,1430GT98.6% 932.8 / 3.3 286R65S (CGT→AGT) yhfXputative pyridoxal 5'‑phosphate binding protein
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (128/154);  total (130/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 290 total aligned reads displayed.

AACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCG  >  CP009273/3503997‑3504288
                                                                                                                                                  |                                                                                                                                                 
 aCCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCCCGGATTACTAcc                                                                                                                                                >  1:636606/1‑149 (MQ=255)
    tgatgaCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCaaa                                                                                                                                             <  2:13515/149‑1 (MQ=255)
      atgaCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACt                                                                                                                                           <  2:1121141/149‑1 (MQ=255)
       tgaCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTg                                                                                                                                          >  1:2718501/1‑149 (MQ=255)
        gaCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGc                                                                                                                                         <  1:4763684/149‑1 (MQ=255)
          cAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCtt                                                                                                                                       >  2:2096515/1‑149 (MQ=255)
           aaGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTg                                                                                                                                      >  2:2294992/1‑149 (MQ=255)
           aaGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTg                                                                                                                                      <  1:3752358/149‑1 (MQ=255)
            aGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTgg                                                                                                                                     >  1:274191/1‑149 (MQ=255)
               gATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGtca                                                                                                                                  <  2:4697459/149‑1 (MQ=255)
               gATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGtca                                                                                                                                  <  1:2450586/149‑1 (MQ=255)
                aTTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGtcat                                                                                                                                 >  1:3227622/1‑149 (MQ=255)
                    gTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGa                                                                                                                             >  1:910760/1‑149 (MQ=255)
                        cAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACa                                                                                                                         <  1:4679580/149‑1 (MQ=255)
                         aGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAg                                                                                                                        >  2:1532739/1‑149 (MQ=255)
                          gATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGt                                                                                                                       <  2:320305/149‑1 (MQ=255)
                          gATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGt                                                                                                                       <  2:4353785/149‑1 (MQ=255)
                          gATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGt                                                                                                                       <  1:1023815/149‑1 (MQ=255)
                            tGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTc                                                                                                                     >  1:1773276/1‑149 (MQ=255)
                            tGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTc                                                                                                                     <  1:3043963/149‑1 (MQ=255)
                              ccccTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCaa                                                                                                                   <  2:1752649/149‑1 (MQ=255)
                                ccTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATg                                                                                                                 <  1:218715/149‑1 (MQ=255)
                                  tGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGcc                                                                                                               >  2:4286088/1‑149 (MQ=255)
                                   gATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCg                                                                                                              >  2:1859355/1‑149 (MQ=255)
                                      gcgcCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTaa                                                                                                           >  1:469178/1‑149 (MQ=255)
                                      gcgcCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTaa                                                                                                           >  1:1727344/1‑149 (MQ=255)
                                          cacaGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGcc                                                                                                       <  1:3115609/149‑1 (MQ=255)
                                           acaGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAACCTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCcg                                                                                                      <  2:1896075/149‑1 (MQ=255)
                                           acaGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCcg                                                                                                      >  1:1238597/1‑149 (MQ=255)
                                            caGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCcgc                                                                                                     >  2:2347913/1‑149 (MQ=255)
                                             aGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACACTGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGAGTACTACCAACCTGCTTGGTCATCAGATACAGTTCAATGCCGTACAGCcgcg                                                                                                    >  1:1403689/1‑149 (MQ=255)
                                              ggCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCgcgc                                                                                                   >  1:4058486/1‑149 (MQ=255)
                                               gCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGcc                                                                                                  <  1:228368/149‑1 (MQ=255)
                                                  aaCCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGtc                                                                                               >  1:1828025/1‑149 (MQ=255)
                                                   aCCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGtct                                                                                              <  2:4222461/149‑1 (MQ=255)
                                                   aCCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGtct                                                                                              <  1:1451683/149‑1 (MQ=255)
                                                    ccAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGtctc                                                                                             <  2:3524241/149‑1 (MQ=255)
                                                      aGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCaa                                                                                           <  2:4661305/149‑1 (MQ=255)
                                                       gcgcGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAAt                                                                                          >  1:1023716/1‑149 (MQ=255)
                                                        cgcgGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATc                                                                                         <  1:2820603/149‑1 (MQ=255)
                                                           ggcgCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGc                                                                                      <  1:3830579/149‑1 (MQ=255)
                                                              gcATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGc                                                                                   >  1:532659/1‑149 (MQ=255)
                                                                  gACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTAc                                                                               >  2:876153/1‑149 (MQ=255)
                                                                  gACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTAc                                                                               >  2:2940219/1‑149 (MQ=255)
                                                                     tCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGt                                                                            <  1:4375773/149‑1 (MQ=255)
                                                                     tCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGt                                                                            <  2:4454448/149‑1 (MQ=255)
                                                                      cgcgCCTCTTTGTAATCCACCGACACAATGCCGCTGTATCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCAGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGtt                                                                           <  2:3656193/149‑1 (MQ=255)
                                                                       gcgcCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGttt                                                                          <  1:3922417/149‑1 (MQ=255)
                                                                        cgcCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGtttt                                                                         <  2:278454/149‑1 (MQ=255)
                                                                         gcCTCTTTGTAATCCACCGCCACCATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCACACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTc                                                                        <  2:3344365/149‑1 (MQ=255)
                                                                          ccTCTTTGTCATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTcc                                                                       <  1:317615/149‑1 (MQ=255)
                                                                          ccTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTcc                                                                       >  2:3992857/1‑149 (MQ=255)
                                                                          ccTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTcc                                                                       <  2:274139/149‑1 (MQ=255)
                                                                          ccTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTcc                                                                       <  1:1510770/149‑1 (MQ=255)
                                                                          ccTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTcc                                                                       >  2:2576059/1‑149 (MQ=255)
                                                                            tctTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAg                                                                     <  2:2920164/149‑1 (MQ=255)
                                                                                tGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATc                                                                 >  2:315422/1‑149 (MQ=255)
                                                                                 ggaaTCCACCGCCACAATGCCGCTGGAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCt                                                                <  2:740765/147‑1 (MQ=255)
                                                                                      ccACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATcc                                                           <  2:3390494/149‑1 (MQ=255)
                                                                                        aCCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCAc                                                         <  2:3887211/149‑1 (MQ=255)
                                                                                        aCCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCAc                                                         >  1:4096744/1‑149 (MQ=255)
                                                                                        aCCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCAc                                                         >  1:452712/1‑149 (MQ=255)
                                                                                          cGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGt                                                       <  2:2663093/149‑1 (MQ=255)
                                                                                          cGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGt                                                       >  1:3522562/1‑149 (MQ=255)
                                                                                              acaATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGAt                                                   <  1:1880541/149‑1 (MQ=255)
                                                                                               caATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATc                                                  <  1:3460769/149‑1 (MQ=255)
                                                                                               caATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATc                                                  <  1:786570/149‑1 (MQ=255)
                                                                                                 aTGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCAc                                                <  2:3566348/149‑1 (MQ=255)
                                                                                                   gCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCAccc                                              >  2:1448268/1‑149 (MQ=255)
                                                                                                      gCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGc                                           <  1:420775/149‑1 (MQ=255)
                                                                                                           aGCCTAATGCCAGAAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTcc                                      <  1:2992043/149‑1 (MQ=255)
                                                                                                            gCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCg                                     >  1:4268168/1‑149 (MQ=255)
                                                                                                              cTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCggg                                   <  2:369266/149‑1 (MQ=255)
                                                                                                              cTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCggg                                   <  1:3806978/149‑1 (MQ=255)
                                                                                                              cTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCggg                                   >  1:1990836/1‑149 (MQ=255)
                                                                                                               tAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCgggg                                  >  2:1275039/1‑149 (MQ=255)
                                                                                                                 aTGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCg                                <  1:1334817/149‑1 (MQ=255)
                                                                                                                   gCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGAt                              >  2:3095143/1‑149 (MQ=255)
                                                                                                                   gCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGAt                              <  2:4314162/149‑1 (MQ=255)
                                                                                                                    ccAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATc                             >  2:2225944/1‑149 (MQ=255)
                                                                                                                     cAGCAATTTTTCCGCCAGCCACGGATTACTACCAACCTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCt                            <  2:2550796/149‑1 (MQ=255)
                                                                                                                     cAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCt                            >  2:3861290/1‑149 (MQ=255)
                                                                                                                     cAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCt                            <  2:551364/149‑1 (MQ=255)
                                                                                                                     cAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCt                            >  1:245075/1‑149 (MQ=255)
                                                                                                                     cAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCt                            >  1:2939569/1‑149 (MQ=255)
                                                                                                                     cAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCt                            <  1:1568270/149‑1 (MQ=255)
                                                                                                                     cAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCt                            <  1:3956935/149‑1 (MQ=255)
                                                                                                                       gCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTg                          >  2:214379/1‑149 (MQ=255)
                                                                                                                               tCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCctgctg                  >  1:2544355/1‑149 (MQ=255)
                                                                                                                                 cGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGcc                >  2:2626230/1‑149 (MQ=255)
                                                                                                                                 cGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGGCCGGGGCGATCTTGCCCTGCTGcc                <  1:262350/149‑1 (MQ=255)
                                                                                                                                    cagccaCGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCcaca             >  1:1634466/1‑149 (MQ=255)
                                                                                                                                    cagccaCGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCcaca             >  1:203524/1‑149 (MQ=255)
                                                                                                                                    cagccaCGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCcaca             >  1:242865/1‑149 (MQ=255)
                                                                                                                                    cagccaCGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCcaca             >  2:2473910/1‑149 (MQ=255)
                                                                                                                                       ccaCGGATTACTACCAATCTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGc          <  2:4544272/149‑1 (MQ=255)
                                                                                                                                       ccaCGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGc          <  1:1718684/149‑1 (MQ=255)
                                                                                                                                        caCGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCt         <  1:2347913/149‑1 (MQ=255)
                                                                                                                                           ggATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAg      >  1:3854357/1‑149 (MQ=255)
                                                                                                                                               tactacCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTgcg  >  1:1274524/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCG  >  CP009273/3503997‑3504288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑