BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,551,133	C→A	6.2%	A102A (GCG→GCT)	rtcB ←	RNA‑splicing ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,551,133	0	C	A	6.2%	1108.9 / 8.5	362	A102A (GCG→GCT)	rtcB	RNA‑splicing ligase
Reads supporting (aligned to +/- strand): ref base C (161/157); new base A (41/3); total (202/160)
Fisher's exact test for biased strand distribution p-value = 1.79e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 368 total aligned reads displayed.

GGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTT  >  CP009273/3550985‑3551281

ggAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATc                                                                                                                                                      <  1:1561632/149‑1 (MQ=255)

  aaaCGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATAGc                                                                                                                                                    >  1:2486364/1‑149 (MQ=255)

   aaCGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGcc                                                                                                                                                   <  2:1896802/149‑1 (MQ=255)

    aCGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCt                                                                                                                                                  <  2:4002105/149‑1 (MQ=255)

     cGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTg                                                                                                                                                 <  2:3664313/149‑1 (MQ=255)

     cGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTg                                                                                                                                                 <  2:2411863/149‑1 (MQ=255)

       gggATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCAGAc                                                                                                                                               >  2:2684626/1‑149 (MQ=255)

          atatTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCa                                                                                                                                            <  1:2188690/149‑1 (MQ=255)

            atTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGc                                                                                                                                          >  2:4161288/1‑149 (MQ=255)

              ttttGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGctc                                                                                                                                        <  2:4425273/149‑1 (MQ=255)

               tttGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAAACGCCAGACGCAGcact                                                                                                                                       >  1:469735/1‑149 (MQ=255)

                ttGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCGTACGCAGCTCTg                                                                                                                                      >  1:2977212/1‑149 (MQ=255)

                  gCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGcc                                                                                                                                    <  2:1478447/149‑1 (MQ=255)

                   cGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCa                                                                                                                                   <  1:2741257/149‑1 (MQ=255)

                    gTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAg                                                                                                                                  >  2:3188679/1‑149 (MQ=255)

                     ttAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAgg                                                                                                                                 <  2:1008942/149‑1 (MQ=255)

                     ttAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAgg                                                                                                                                 <  1:2861893/149‑1 (MQ=255)

                     ttAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTGTCAATCGCCTGACGCAGCTCTGCCAgg                                                                                                                                 >  1:4410848/1‑149 (MQ=255)

                        aCCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCCGCTCTGCCAGGgtt                                                                                                                              >  2:524733/1‑149 (MQ=255)

                        aCCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGttt                                                                                                                              <  2:2923558/149‑1 (MQ=255)

                         ccACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTGTCAATCGCCAGACGCAGCTCTGCCAGGtttt                                                                                                                             >  1:1754670/1‑149 (MQ=255)

                              gATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTaaggc                                                                                                                        >  1:1870994/1‑149 (MQ=255)

                                 aaCCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCCGGTTTTcaggccgg                                                                                                                     >  2:3820715/1‑149 (MQ=255)

                                 aaCCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTcaggcagg                                                                                                                     >  2:1320884/1‑149 (MQ=255)

                                  aCCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATAGCCAGACGCAGCTCTGCCAGGTTTTCAGGCAGGt                                                                                                                    >  2:1607044/1‑149 (MQ=255)

                                   ccGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTc                                                                                                                   <  2:832524/149‑1 (MQ=255)

                                       cTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTcc                                                                                                               <  1:2909147/149‑1 (MQ=255)

                                       cTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTcc                                                                                                               <  1:1505656/149‑1 (MQ=255)

                                        ttCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTccg                                                                                                              >  2:3095011/1‑149 (MQ=255)

                                        ttCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATAGCCAGACCCAGCACTGCCACGTTTTAAGGCAGGTCttacc                                                                                                              >  1:2114194/1‑146 (MQ=255)

                                          cAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATAGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTacgcc                                                                                                            >  1:3131417/1‑149 (MQ=255)

                                            aGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGt                                                                                                          <  1:381199/149‑1 (MQ=255)

                                            aGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGt                                                                                                          <  1:1998030/149‑1 (MQ=255)

                                             gCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTAAATCGCCAGTCCCCGCTCTGCCCCGTTTTAAGGCAGGTCGTacgccggt                                                                                                         >  1:434703/1‑147 (MQ=255)

                                               tCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATAGCCGGAAGCACCGCAGCCACGTTTTAATGCAGGTATTAAGCCGGTaa                                                                                                       >  1:3603598/1‑149 (MQ=255)

                                               tCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTGTCAAAAGCCTGAAGCCCCTCGGCCAGGTTTTGAGGCAGGTCTTacgcccgtaa                                                                                                       >  1:1214419/1‑144 (MQ=255)

                                                     cATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGt                                                                                                 >  2:3120548/1‑149 (MQ=255)

                                                      aTTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTa                                                                                                >  1:712122/1‑149 (MQ=255)

                                                       gttAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTAc                                                                                               <  2:4555458/148‑1 (MQ=255)

                                                              tCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAgcgc                                                                                        <  1:3494604/149‑1 (MQ=255)

                                                              tCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCACTGCCACGTTTTAAGGCAGGTCTTCCGCCGTTAAAGCGGTACGCAgcgc                                                                                        >  2:3258786/1‑149 (MQ=255)

                                                                gACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGt                                                                                      <  2:2082346/149‑1 (MQ=255)

                                                                    ttAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCAt                                                                                  >  2:2475396/1‑149 (MQ=255)

                                                                    ttAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTAAGGCAGGTCTTCCGCCGTTAAAGCGGTACGCAGCGCGTTCAt                                                                                  >  2:233903/1‑149 (MQ=255)

                                                                       aCAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTcc                                                                               >  2:4339688/1‑149 (MQ=255)

                                                                       aCAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTcc                                                                               <  2:484567/149‑1 (MQ=255)

                                                                           gTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAg                                                                           <  1:3541036/149‑1 (MQ=255)

                                                                           gTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCAGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAg                                                                           >  1:4755060/1‑149 (MQ=255)

                                                                               aTTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCaa                                                                       >  2:767208/1‑149 (MQ=255)

                                                                               aTTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCaa                                                                       <  1:1093720/149‑1 (MQ=255)

                                                                               aTTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCaa                                                                       <  1:1162100/149‑1 (MQ=255)

                                                                                ttttCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAat                                                                      <  2:2179122/149‑1 (MQ=255)

                                                                                ttttCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATAGCCTGAAGCAGCACTGCCAGGTTTTAAGGCAGGTCTTACGCCGTTTAACCGGTACGCAGCGCGTTAATTAAAAAGCCaaa                                                                      >  1:3316530/1‑148 (MQ=255)

                                                                                ttttCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTGTCAAACGCCCGACCCCGCACTGCCCCGTTTTAAGGCAGGTCTTACGCCCGTAAAGCGGTACCCAGCGCGGTAATTACACAccccaa                                                                      >  1:839039/1‑146 (MQ=255)

                                                                                   tCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTACGCCGTTAACGCGGTACGCAGCGCGTTCAATCCACAGCCAAAATc                                                                   >  1:1953164/1‑149 (MQ=255)

                                                                                   tCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTAAAAAGCCGGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTACGCCGTTAAAGCGGTACGCAGCGCGTTCAATCCACAccccaaaaa                                                                   >  1:2819470/1‑143 (MQ=255)

                                                                                    cccAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCAGACCCAGCAATGCCAGGTTTTAAGGCAGGTCTTCCGCCGTTAACGCGGGACGCAGCGCGGTCATTCCACAGCCCAAATcc                                                                  >  1:4503609/1‑149 (MQ=255)

                                                                                      cAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCAc                                                                <  2:339014/149‑1 (MQ=255)

                                                                                       aGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACg                                                               >  2:4031812/1‑149 (MQ=255)

                                                                                         gCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCAGACGCAGCTCTGCCCGGTTTTAAGGCAGGTCTTCCGCCGTTAACGCGGGACGCAGCGCGTTCATTCCACAGCCAAAATCCAcccc                                                             >  1:3331417/1‑149 (MQ=255)

                                                                                          cACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAAAAGCCTGACGCAGCTCTGCCAGGTTTTAAGGCAGGTCTTACCCCGGTAACGCGGTACGCCGCGCGGTCATTCCACAGCCAATAACCACcccc                                                            >  1:93232/1‑149 (MQ=255)

                                                                                            ccTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCAGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCCGCGCGTTCATTCCACAGCCAATATCCACGCCCAc                                                          >  1:2704420/1‑149 (MQ=255)

                                                                                            ccTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCAGACGCAGCTCTGCCAGGTTGTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAAAATCCACGccccc                                                          >  2:4652402/1‑147 (MQ=255)

                                                                                              tttATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCCATATCCACGCCCAccg                                                        >  2:2037106/1‑149 (MQ=255)

                                                                                                tATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCAccgcc                                                      <  2:4384337/149‑1 (MQ=255)

                                                                                                  tCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCAccgccgc                                                    <  1:1302210/149‑1 (MQ=255)

                                                                                                    aCGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCAccgccgccg                                                  >  2:1140798/1‑149 (MQ=255)

                                                                                                    aCGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATAGCCGGACGCCGCTCGGCCCGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGGTCATTCCACACCCAAAAAACACCCcccccgcccccg                                                  >  1:4148935/1‑149 (MQ=255)

                                                                                                      gTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAAACGCCAGACGCAGCTCTTCCAGGTTTTCAGGCAGGTAATCCGCCGTTAAAGCGGTACGCACCGCGTTCATTACACAGCCAAAATCCACGCCCACCCCCGCCGGa                                                >  1:3075844/1‑149 (MQ=255)

                                                                                                            aaCGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATc                                          >  2:4601213/1‑149 (MQ=255)

                                                                                                            aaCGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATc                                          >  2:882264/1‑149 (MQ=255)

                                                                                                             aCGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCg                                         >  2:1193624/1‑149 (MQ=255)

                                                                                                                gCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGccc                                      <  1:4031812/149‑1 (MQ=255)

                                                                                                                 ccAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGcccc                                     <  2:2736815/149‑1 (MQ=255)

                                                                                                                  cAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCt                                    <  2:4730825/149‑1 (MQ=255)

                                                                                                                  cAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCt                                    <  2:3541417/149‑1 (MQ=255)

                                                                                                                  cAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCt                                    <  2:3234456/149‑1 (MQ=255)

                                                                                                                  cAGTGGTACGCCCGTGCGGCACGGCCGTTTAAATCGCCTGTCGCAGCTATGCCAGGGTTTCAGGCCGGTCTTCCGCCGTTAACGCGGTACGCCGCGCGTTCAATCCACCGCCACTATCCACGCCCACCGCCGCCGGCAAAATCGCCCCt                                    >  2:846904/1‑149 (MQ=255)

                                                                                                                   aGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCtt                                   <  2:2395044/149‑1 (MQ=255)

                                                                                                                   aGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCtt                                   <  1:1024915/149‑1 (MQ=255)

                                                                                                                   aGTGGTACGCCCGTGCGGCACGGCCGTTTCAATAGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGGTAACGCGGTACGCAGCGCGTTCAATCCCCAGCCAAAATCCACGCCCCCCCCCGCCGGAATAATCGccccgt                                   >  1:3785632/1‑147 (MQ=255)

                                                                                                                         aCGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCg                             >  2:4351843/1‑149 (MQ=255)

                                                                                                                         aCGCCCGTGCGGCACGGCCGTTTCAATAGCCAGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTACACAGCCAAAAACCACCCCCACCCCCGCCGGAATAATCGCCCCGTTGGTCg                             >  1:623093/1‑149 (MQ=255)

                                                                                                                          cGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCgg                            <  1:3895150/149‑1 (MQ=255)

                                                                                                                           gCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCGCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGGTAACGCGGTACGCCGCGCGTTCATTCCACAGCCCATAACCCCCCCCCCCGCCGCCCGAATAATCGCCCCTTTGGTCggg                           >  2:4057295/1‑149 (MQ=255)

                                                                                                                             ccGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGAt                         <  1:1705111/149‑1 (MQ=255)

                                                                                                                             ccGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGAt                         >  2:1929415/1‑149 (MQ=255)

                                                                                                                                    gCACGGCCGTTTCAATAGCCAGACGCAGCTCTGCCAGGTTTTAAGGCAGGTCTTACGCCGTTAACGCGGTACGCAGCGCGTTAATTCCACAGCCAAAATACACGCCCACCGCCGCCGGGATAATAGCCCCGTTGGTCGGGATAACGCTa                  >  2:1313616/1‑149 (MQ=255)

                                                                                                                                      aCGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTAcc                <  1:1136787/149‑1 (MQ=255)

                                                                                                                                         gCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCGTTGGTCGGGATCACGCTACCAAt             >  2:2881319/1‑149 (MQ=255)

                                                                                                                                          ccGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAAtg            >  2:4195940/1‑149 (MQ=255)

                                                                                                                                           cGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATCATCGCCCCGTTGGTCGGGATCACGCTACCAAtgg           >  2:922169/1‑149 (MQ=255)

                                                                                                                                           cGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAAtgg           >  2:2113419/1‑149 (MQ=255)

                                                                                                                                            gTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAAtggt          >  2:1838425/1‑149 (MQ=255)

                                                                                                                                            gTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAAtggt          >  1:4770760/1‑149 (MQ=255)

                                                                                                                                              ttCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCATGCTACCAAtggtgg        >  1:4594922/1‑149 (MQ=255)

                                                                                                                                               tCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGa       >  1:2349184/1‑149 (MQ=255)

                                                                                                                                               tCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGa       <  1:1121610/149‑1 (MQ=255)

                                                                                                                                                 aaTCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAAc     <  2:4504334/149‑1 (MQ=255)

                                                                                                                                                  aTCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAAcc    >  2:4255395/1‑149 (MQ=255)

GGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACGCGGTACGCAGCGCGTTCATTCCACAGCCAATATCCACGCCCACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTT  >  CP009273/3550985‑3551281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`