Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,561,574 C→A 100% W372L (TGG→TTG)  glgC ← glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,561,5740CA95.4% 790.8 / 27.5 261W372L (TGG→TTG) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (5/7);  new base A (125/124);  total (130/131)
Fisher's exact test for biased strand distribution p-value = 7.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 261 total aligned reads displayed.

TTTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACC  >  CP009273/3561428‑3561719
                                                                                                                                                  |                                                                                                                                                 
     cgcgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTc                                                                                                                                            <  2:1127913/149‑1 (MQ=255)
       cgTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCg                                                                                                                                          <  1:1398125/149‑1 (MQ=255)
         ttACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGt                                                                                                                                        >  2:2551244/1‑149 (MQ=255)
          tACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTa                                                                                                                                       <  2:3509675/149‑1 (MQ=255)
          tACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTa                                                                                                                                       >  1:2753681/1‑149 (MQ=255)
            ccAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAAc                                                                                                                                     >  1:3651655/1‑149 (MQ=255)
                    aggCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcggc                                                                                                                             <  1:2817434/147‑1 (MQ=255)
                     tGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcggcg                                                                                                                            >  2:560568/1‑149 (MQ=255)
                       ccTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGa                                                                                                                          >  1:3815111/1‑149 (MQ=255)
                       ccTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGa                                                                                                                          >  2:2899582/1‑149 (MQ=255)
                        cttcttCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGaa                                                                                                                         <  1:4706075/149‑1 (MQ=255)
                        cttcttCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGaa                                                                                                                         <  1:1760557/149‑1 (MQ=255)
                         ttcttcTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAAt                                                                                                                        >  1:1185197/1‑149 (MQ=255)
                          tcttctGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGACTc                                                                                                                       >  2:367246/1‑149 (MQ=255)
                          tcttctGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATc                                                                                                                       >  2:3075246/1‑149 (MQ=255)
                           cttctGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCa                                                                                                                      >  1:583913/1‑149 (MQ=255)
                             tctGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAAt                                                                                                                    <  1:1953497/149‑1 (MQ=255)
                               tGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGt                                                                                                                  >  2:1737919/1‑149 (MQ=255)
                                gAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATTGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGtt                                                                                                                 >  1:2635614/1‑149 (MQ=255)
                                gAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGtt                                                                                                                 >  1:2323528/1‑149 (MQ=255)
                                  aCGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGc                                                                                                               <  1:3582601/149‑1 (MQ=255)
                                  aCGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGc                                                                                                               <  2:4659290/149‑1 (MQ=255)
                                  aCGATAGAAACGACGAGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACACGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACAGCGGAATCAATGTTGc                                                                                                               <  2:2346847/149‑1 (MQ=255)
                                   cGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCa                                                                                                              <  1:3840005/149‑1 (MQ=255)
                                    gATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAg                                                                                                             <  1:1451678/149‑1 (MQ=255)
                                     aTAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAga                                                                                                            >  1:2199367/1‑149 (MQ=255)
                                      tAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGTGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaa                                                                                                           >  2:1897274/1‑149 (MQ=255)
                                      tAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGTGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaa                                                                                                           >  2:2218350/1‑149 (MQ=255)
                                      tAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaa                                                                                                           >  1:208928/1‑149 (MQ=255)
                                       aGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAAGACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaat                                                                                                          <  1:1534482/149‑1 (MQ=255)
                                           acgacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatgaat                                                                                                      >  1:3872772/1‑149 (MQ=255)
                                            cgacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAAtt                                                                                                     <  1:4121902/149‑1 (MQ=255)
                                             gacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTc                                                                                                    <  2:3599245/149‑1 (MQ=255)
                                             gacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTc                                                                                                    >  2:3762235/1‑149 (MQ=255)
                                              acgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCa                                                                                                   <  2:3925728/149‑1 (MQ=255)
                                               cgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAc                                                                                                  <  2:2418977/149‑1 (MQ=255)
                                                 tGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcgc                                                                                                <  1:1685378/149‑1 (MQ=255)
                                                    aTCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGACTGAATTCACGCGaa                                                                                             >  2:3704641/1‑149 (MQ=255)
                                                    aTCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGaa                                                                                             <  2:1501092/149‑1 (MQ=255)
                                                    aTCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGaa                                                                                             >  2:16058/1‑149 (MQ=255)
                                                    aTCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGaa                                                                                             >  1:2451895/1‑149 (MQ=255)
                                                     tCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAc                                                                                            >  1:3279131/1‑149 (MQ=255)
                                                     tCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAc                                                                                            <  2:1620611/149‑1 (MQ=255)
                                                       ttCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcgc                                                                                          <  2:933391/149‑1 (MQ=255)
                                                          ctctGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcgcgcg                                                                                       <  1:750116/149‑1 (MQ=255)
                                                             tGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCgaga                                                                                    >  1:4388093/1‑149 (MQ=255)
                                                                gTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaaca                                                                                 <  2:1680667/149‑1 (MQ=255)
                                                                 ttttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATTAATTCACGCGAACGCGCGagaacag                                                                                >  2:739641/1‑149 (MQ=255)
                                                                 ttttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACCGCGCGagaacag                                                                                >  2:410059/1‑149 (MQ=255)
                                                                    tCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacagaac                                                                             <  1:3704801/149‑1 (MQ=255)
                                                                     cACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACg                                                                            <  2:4569066/149‑1 (MQ=255)
                                                                      aCCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACgg                                                                           <  2:84643/149‑1 (MQ=255)
                                                                       ccAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGa                                                                          <  1:346405/149‑1 (MQ=255)
                                                                        cAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGAc                                                                         >  1:4559243/1‑149 (MQ=255)
                                                                          aTCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGACCAGAACGGACTg                                                                       >  2:2426465/1‑149 (MQ=255)
                                                                                   ccTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcaccaccac                                                              >  1:723332/1‑149 (MQ=255)
                                                                                    cTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCaccaccacc                                                             <  1:874986/149‑1 (MQ=255)
                                                                                     ttCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCg                                                            >  2:3368404/1‑149 (MQ=255)
                                                                                       ccGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGaa                                                          >  1:3556138/1‑149 (MQ=255)
                                                                                       ccGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGaa                                                          >  1:1168260/1‑149 (MQ=255)
                                                                                        cGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAc                                                         <  2:1070999/149‑1 (MQ=255)
                                                                                        cGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAc                                                         <  2:901011/149‑1 (MQ=255)
                                                                                        cGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAc                                                         <  1:2165938/149‑1 (MQ=255)
                                                                                          gAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCg                                                       >  1:4741224/1‑149 (MQ=255)
                                                                                               acacAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGAt                                                  <  1:4003680/149‑1 (MQ=255)
                                                                                               acacAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGAt                                                  <  1:2988913/149‑1 (MQ=255)
                                                                                                cacaAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATc                                                 >  2:220207/1‑149 (MQ=255)
                                                                                                cacaAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATc                                                 >  2:3271595/1‑149 (MQ=255)
                                                                                                   aaGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcaca                                              >  1:3663440/1‑149 (MQ=255)
                                                                                                    aGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacac                                             >  2:4108360/1‑149 (MQ=255)
                                                                                                       aCGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAc                                          >  2:2749116/1‑149 (MQ=255)
                                                                                                          atcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccgc                                       >  1:481342/1‑149 (MQ=255)
                                                                                                           tcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccgcc                                      <  1:1801933/149‑1 (MQ=255)
                                                                                                             gatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCgg                                    <  1:789854/149‑1 (MQ=255)
                                                                                                              atGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGa                                   >  1:2335408/1‑149 (MQ=255)
                                                                                                              atGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGa                                   <  2:4474318/149‑1 (MQ=255)
                                                                                                               tGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGaa                                  >  1:3357581/1‑149 (MQ=255)
                                                                                                               tGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGaa                                  >  1:1490178/1‑149 (MQ=255)
                                                                                                                  cGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACCGCGCGAGACCAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAAcc                               >  2:3227378/1‑149 (MQ=255)
                                                                                                                    cAGCGGTGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAg                             >  1:258736/1‑149 (MQ=255)
                                                                                                                            cAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTaa                     <  2:4344628/149‑1 (MQ=255)
                                                                                                                             aGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAg                    <  1:650013/149‑1 (MQ=255)
                                                                                                                              gACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAgg                   >  2:2012749/1‑149 (MQ=255)
                                                                                                                              gACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAgg                   >  1:1866373/1‑149 (MQ=255)
                                                                                                                              gACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAgg                   >  1:2175029/1‑149 (MQ=255)
                                                                                                                              gACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAgg                   >  2:606369/1‑149 (MQ=255)
                                                                                                                               aCGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAggg                  <  2:1185084/149‑1 (MQ=255)
                                                                                                                                cGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGt                 <  1:3019093/149‑1 (MQ=255)
                                                                                                                                    aCGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATc             >  1:2308850/1‑149 (MQ=255)
                                                                                                                                     cGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACCGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATcc            >  2:1381541/1‑149 (MQ=255)
                                                                                                                                     cGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATcc            >  2:1381421/1‑149 (MQ=255)
                                                                                                                                      gAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATccc           <  1:1020768/149‑1 (MQ=255)
                                                                                                                                      gAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATccc           <  1:1585375/149‑1 (MQ=255)
                                                                                                                                      gAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATccc           <  2:4652703/149‑1 (MQ=255)
                                                                                                                                      gAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATccc           <  1:2448477/149‑1 (MQ=255)
                                                                                                                                      gAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATccc           >  1:4258439/1‑149 (MQ=255)
                                                                                                                                         cGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTg        <  1:2226349/149‑1 (MQ=255)
                                                                                                                                           aCCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGc      <  1:1008411/149‑1 (MQ=255)
                                                                                                                                              tACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTAc   >  2:1976998/1‑149 (MQ=255)
                                                                                                                                               aCCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTAcc  >  2:2664273/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACC  >  CP009273/3561428‑3561719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑