Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,587,949 C→A 8.0% E298* (GAA→TAA)  livM ← leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,587,9490CA 8.0% 978.3 / 38.0 263E298* (GAA→TAA) livMleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (119/123);  new base A (5/16);  total (124/139)
Fisher's exact test for biased strand distribution p-value = 3.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 266 total aligned reads displayed.

GATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTATTTCAGGCCAAAGAAAT  >  CP009273/3587801‑3588095
                                                                                                                                                    |                                                                                                                                                  
   tCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATc                                                                                                                                                 <  2:1704910/149‑1 (MQ=255)
    cGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCt                                                                                                                                                >  2:4370979/1‑149 (MQ=255)
      gCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTc                                                                                                                                              >  1:2199322/1‑149 (MQ=255)
      gCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTc                                                                                                                                              >  2:3400819/1‑149 (MQ=255)
      gCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTc                                                                                                                                              >  1:732825/1‑149 (MQ=255)
        aaaGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGTTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCAc                                                                                                                                            <  1:577433/149‑1 (MQ=255)
          aGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCTAACGGCAGGCGATTTCATCTTCACGc                                                                                                                                          >  1:1852519/1‑149 (MQ=255)
           ggTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCa                                                                                                                                         >  1:439456/1‑149 (MQ=255)
            gTGATGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCaa                                                                                                                                        <  1:4589689/149‑1 (MQ=255)
              gAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACg                                                                                                                                      >  1:2884156/1‑149 (MQ=255)
                aGGATTCCGGGATGACAACGCCCTGACGCGCCGCAAAAAGCGTTCCGGAAAAACCGCAAAACTCGGAAATTATGGTAAAGGCAGTCAGTTTGATACTACGCGGGTTTAAGCCCTGCTAACGGCAGGCGATTTCATCTTCACGCAACGCt                                                                                                                                    <  1:4171461/149‑1 (MQ=255)
                 ggATTCAGGGCTGACAAAGCACTGACGCGCCTCAAAAAGAGTTCCGTAAAAACCGGCAAACGCGGCACTTATGTTAATGTCTTTAAGCTTGTTACGACGCTTGATTATGCCTAGCGAACGGCAGGCGATTTCATCTTATAGGAACGCtt                                                                                                                                   <  2:2603087/149‑1 (MQ=255)
                  gagtCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTGATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTc                                                                                                                                  <  2:1294113/146‑1 (MQ=255)
                  gATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTc                                                                                                                                  >  1:2583761/1‑149 (MQ=255)
                  gATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTc                                                                                                                                  <  2:3684311/149‑1 (MQ=255)
                  gATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTc                                                                                                                                  >  2:11321/1‑149 (MQ=255)
                    ttCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTccc                                                                                                                                >  2:3074873/1‑149 (MQ=255)
                    ttCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTccc                                                                                                                                <  1:1562692/149‑1 (MQ=255)
                    ttCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTccc                                                                                                                                >  1:1433972/1‑149 (MQ=255)
                       cGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTAATCTTCACGCAACGCTTCCcacg                                                                                                                             <  2:662388/149‑1 (MQ=255)
                            tGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCacgcacgc                                                                                                                        >  1:394930/1‑149 (MQ=255)
                             gACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGcc                                                                                                                       >  1:2006121/1‑149 (MQ=255)
                              aCAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAACCGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGccc                                                                                                                      <  1:4463989/149‑1 (MQ=255)
                              aCAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGccc                                                                                                                      >  1:873678/1‑149 (MQ=255)
                              aCAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTAATCTTCACGCAACGCTTCCCACGCACGccc                                                                                                                      <  2:3353247/149‑1 (MQ=255)
                               cAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGcccc                                                                                                                     >  2:4184509/1‑149 (MQ=255)
                                 aaGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAg                                                                                                                   <  2:4611030/149‑1 (MQ=255)
                                 aaGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAg                                                                                                                   >  2:1400190/1‑149 (MQ=255)
                                  aGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGc                                                                                                                  >  2:3286765/1‑149 (MQ=255)
                                  aGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGc                                                                                                                  >  2:138115/1‑149 (MQ=255)
                                  aGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACGTAGGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCCCGCAACGCGTCCCCCGCACGCCCCAGc                                                                                                                  <  2:4468269/149‑1 (MQ=255)
                                   gCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTAATCTTCACGCAACGCTTCCCACGCACGCCCCAGCg                                                                                                                 <  1:4334072/149‑1 (MQ=255)
                                     ccTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGc                                                                                                               <  2:1715565/149‑1 (MQ=255)
                                     ccTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGc                                                                                                               >  1:3569713/1‑149 (MQ=255)
                                       tGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCAt                                                                                                             <  1:659890/149‑1 (MQ=255)
                                        gACGCGCCGCAAACCGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATc                                                                                                            <  2:6395/149‑1 (MQ=255)
                                        gACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATc                                                                                                            <  1:1252890/149‑1 (MQ=255)
                                        gACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATc                                                                                                            >  1:1245997/1‑149 (MQ=255)
                                                 cAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGTCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAgg                                                                                                   >  1:101109/1‑149 (MQ=255)
                                                   aaCAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCg                                                                                                 >  2:1000894/1‑149 (MQ=255)
                                                     cAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGt                                                                                               <  1:3530748/149‑1 (MQ=255)
                                                     cAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGt                                                                                               >  1:3396817/1‑149 (MQ=255)
                                                     cAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGt                                                                                               <  2:2427784/149‑1 (MQ=255)
                                                      aGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGtt                                                                                              <  1:1687780/149‑1 (MQ=255)
                                                       gCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTa                                                                                             >  1:3668904/1‑149 (MQ=255)
                                                       gCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTa                                                                                             >  2:4661937/1‑149 (MQ=255)
                                                         gTTCCGGCAAAACCGGCAAACGCGGCACAAAAGGAAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAAt                                                                                           >  1:961629/1‑149 (MQ=255)
                                                            ccGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGAc                                                                                        >  2:3638298/1‑149 (MQ=255)
                                                              ggaaaaaCCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATATTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACaa                                                                                      <  1:3566520/146‑1 (MQ=255)
                                                              ggCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACaa                                                                                      <  1:3761768/149‑1 (MQ=255)
                                                                cAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAAc                                                                                    >  2:378616/1‑149 (MQ=255)
                                                                  aaaCGGGCAAACGCGTCTCTTTTGTTAAAGGAAGTCAGCTTGATACGAGGCGGGCTTAAGCCCAGCTAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAg                                                                                  <  1:4063980/149‑1 (MQ=255)
                                                                  aaaCCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAg                                                                                  <  1:4248206/149‑1 (MQ=255)
                                                                   aaCCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAACGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAgg                                                                                 >  2:2141756/1‑149 (MQ=255)
                                                                   aaCCGGCAAACGCGGAACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAgg                                                                                 <  1:4433187/149‑1 (MQ=255)
                                                                       ggCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTa                                                                             <  1:11321/149‑1 (MQ=255)
                                                                        gCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAg                                                                            <  2:2983079/149‑1 (MQ=255)
                                                                        gCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAg                                                                            >  2:2275204/1‑149 (MQ=255)
                                                                          aaaCGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGca                                                                          >  1:3933639/1‑149 (MQ=255)
                                                                           aaCGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGcac                                                                         >  1:799061/1‑149 (MQ=255)
                                                                                ggCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCAccacca                                                                    >  1:4543119/1‑149 (MQ=255)
                                                                                ggCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTAATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCAccacca                                                                    >  2:2259544/1‑149 (MQ=255)
                                                                                 gCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACcag                                                                   >  2:2873612/1‑149 (MQ=255)
                                                                                      tATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTAATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAgcagca                                                              <  1:3182318/149‑1 (MQ=255)
                                                                                        tGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAAc                                                            <  2:938457/149‑1 (MQ=255)
                                                                                          gTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGc                                                          <  1:3141123/149‑1 (MQ=255)
                                                                                             aaGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGccac                                                       >  2:2904694/1‑149 (MQ=255)
                                                                                             aaGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGccac                                                       >  1:159125/1‑149 (MQ=255)
                                                                                             aaGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTAATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGccac                                                       >  1:2101487/1‑149 (MQ=255)
                                                                                                  aGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGt                                                  <  1:995523/149‑1 (MQ=255)
                                                                                                  aGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTAATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGt                                                  <  2:2471568/149‑1 (MQ=255)
                                                                                                   gTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTa                                                 >  1:4694611/1‑149 (MQ=255)
                                                                                                      aGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTagag                                              <  2:2014164/149‑1 (MQ=255)
                                                                                                      aGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTagag                                              >  1:271261/1‑149 (MQ=255)
                                                                                                       gCTTGATACGACGCGGGCTTAAGCCCAGCGCACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAgg                                             <  1:3314199/149‑1 (MQ=255)
                                                                                                               cgacgCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGac                                     <  1:3487750/149‑1 (MQ=255)
                                                                                                                gacgCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGaca                                    <  1:348617/149‑1 (MQ=255)
                                                                                                                 acgCGGTATTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGacac                                   <  1:818848/149‑1 (MQ=255)
                                                                                                                 acgCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGacac                                   <  1:1737427/149‑1 (MQ=255)
                                                                                                                 acgCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGacac                                   <  1:2026380/149‑1 (MQ=255)
                                                                                                                     gggCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATc                               >  1:1762122/1‑149 (MQ=255)
                                                                                                                     gggCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATc                               >  1:721886/1‑149 (MQ=255)
                                                                                                                         ttAAGCCCAGCGAACGGCAGGCGATTTAATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAg                           <  1:2754362/149‑1 (MQ=255)
                                                                                                                          tAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAgg                          >  2:2864902/1‑149 (MQ=255)
                                                                                                                           aaGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAggg                         >  1:585291/1‑149 (MQ=255)
                                                                                                                             gCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGAt                       >  2:2880857/1‑149 (MQ=255)
                                                                                                                             gCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGAt                       <  2:2640937/149‑1 (MQ=255)
                                                                                                                              cccAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATc                      <  2:2557638/149‑1 (MQ=255)
                                                                                                                                cAGCGCACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGt                    <  1:2408475/149‑1 (MQ=255)
                                                                                                                                 aGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTa                   <  2:1885767/149‑1 (MQ=255)
                                                                                                                                 aGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTa                   >  1:3849680/1‑149 (MQ=255)
                                                                                                                                  gCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTAt                  <  1:3074873/149‑1 (MQ=255)
                                                                                                                                  gCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTAt                  <  1:3339890/149‑1 (MQ=255)
                                                                                                                                       cGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTATTTCAg             <  1:2758432/149‑1 (MQ=255)
                                                                                                                                       cGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTATTTCAg             <  1:1370359/149‑1 (MQ=255)
                                                                                                                                         gCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTATTTCAGGc           <  2:4443953/149‑1 (MQ=255)
                                                                                                                                         gCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTATTTCAGGc           >  2:503346/1‑149 (MQ=255)
                                                                                                                                          cAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTATTTCAGGcc          <  1:4770403/149‑1 (MQ=255)
                                                                                                                                           aGGCGATTTAATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTATTTCAGGCCa         <  1:1858075/149‑1 (MQ=255)
                                                                                                                                            ggCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTATTTCAGGCCaa        >  1:845348/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAACAGCGTTCCGGCAAAACCGGCAAACGCGGCACTTATGGTAAAGGCAGTCAGCTTGATACGACGCGGGCTTAAGCCCAGCGAACGGCAGGCGATTTCATCTTCACGCAACGCTTCCCACGCACGCCCCAGCGGCATCCGCAGCAGGCGGTTAATGACAAACAGGCTTAGCACCACCAGCAGCAACGCCACCAGGTAGAGGAAGATGACACGATCGGAGGGATCGTATTTCAGGCCAAAGAAAT  >  CP009273/3587801‑3588095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑