Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,598,440 C→A 88.6% N34K (AAC→AAA)  yhhL → DUF1145 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,598,4400CA88.6% 633.7 / 75.3 248N34K (AAC→AAA) yhhLDUF1145 family protein
Reads supporting (aligned to +/- strand):  ref base C (13/16);  new base A (109/110);  total (122/126)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAGA  >  CP009273/3598295‑3598585
                                                                                                                                                 |                                                                                                                                                 
 gTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTgg                                                                                                                                               <  1:239860/149‑1 (MQ=255)
 gTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTgg                                                                                                                                               >  2:4721839/1‑149 (MQ=255)
     cTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCt                                                                                                                                           >  2:2625775/1‑149 (MQ=255)
      ttATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTg                                                                                                                                          >  1:3611388/1‑149 (MQ=255)
       tATCGGCTGTATCAACGCGAAGCACAAGGAGATAGTGTTGCTTATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATATTCGTTAACGTGGCGCTGa                                                                                                                                         <  1:4280270/149‑1 (MQ=255)
         tCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGAtt                                                                                                                                       >  2:1367997/1‑149 (MQ=255)
           ggCTGTATCCACGCGAAGCACAAGGACAAAGAGATGCTGATTAATATTGGTCGTTTGTAAATGCTCTGCGTTAGGGGATTTTTAATCCTCAACCTGCTGCATCCCTTCCCACGCCCACAGAATATCTACGTTAAAGAGGCTCTGAattt                                                                                                                                     >  2:3394288/1‑149 (MQ=255)
             cTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTa                                                                                                                                   <  1:326960/149‑1 (MQ=255)
             cTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTa                                                                                                                                   <  2:2906944/149‑1 (MQ=255)
              tGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTAc                                                                                                                                  >  2:2279825/1‑149 (MQ=255)
              tGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTAc                                                                                                                                  <  1:2479014/149‑1 (MQ=255)
               gTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTaac                                                                                                                                 >  1:2179092/1‑147 (MQ=255)
                tATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCg                                                                                                                                <  2:1598378/149‑1 (MQ=255)
                 aTCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGt                                                                                                                               >  1:918654/1‑149 (MQ=255)
                   cAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGc                                                                                                                             <  1:2029888/149‑1 (MQ=255)
                    aaCGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCt                                                                                                                            <  1:453972/149‑1 (MQ=255)
                      cgcgAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGa                                                                                                                          <  1:3332179/149‑1 (MQ=255)
                      cgcgAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGa                                                                                                                          >  2:433949/1‑149 (MQ=255)
                       gcgAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGAt                                                                                                                         <  2:2451662/149‑1 (MQ=255)
                             cacaAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATgg                                                                                                                   <  1:1456864/149‑1 (MQ=255)
                              tcaAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGt                                                                                                                  <  1:2882344/148‑1 (MQ=255)
                              acaAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGt                                                                                                                  >  2:586117/1‑149 (MQ=255)
                                aaGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTAt                                                                                                                <  1:4038891/149‑1 (MQ=255)
                                aaGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTAt                                                                                                                >  1:4613235/1‑149 (MQ=255)
                                 aGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATg                                                                                                               <  1:2110806/149‑1 (MQ=255)
                                 aGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATg                                                                                                               <  2:1303365/149‑1 (MQ=255)
                                  ggAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGc                                                                                                              >  1:353734/1‑149 (MQ=255)
                                   gagaAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTAACGTGCTGATGCATGGTATGCa                                                                                                             >  2:3666021/1‑149 (MQ=255)
                                    agaAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATACTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTTACGTGCTGATTCATGGTATTCAg                                                                                                            >  1:4014203/1‑149 (MQ=255)
                                     gataGTGATGGTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCGCGCTGTATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGc                                                                                                           <  2:4567691/146‑1 (MQ=255)
                                     gaAAGTGATGCTGATTAATATTGGTCGTTGGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGc                                                                                                           <  2:3425282/149‑1 (MQ=255)
                                      aaaGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCt                                                                                                          <  1:2995605/149‑1 (MQ=255)
                                      aaaGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCt                                                                                                          <  1:4748033/149‑1 (MQ=255)
                                         gTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGgc                                                                                                       <  2:4752009/149‑1 (MQ=255)
                                            aTGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCt                                                                                                    <  2:1778588/149‑1 (MQ=255)
                                            aTGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCt                                                                                                    <  1:3147551/149‑1 (MQ=255)
                                               cTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTAtt                                                                                                 <  1:4729963/149‑1 (MQ=255)
                                                tGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTg                                                                                                >  1:1070450/1‑149 (MQ=255)
                                                tGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTg                                                                                                >  2:4409961/1‑149 (MQ=255)
                                                 gATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGa                                                                                               <  2:2870288/149‑1 (MQ=255)
                                                 gATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCATCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTAGTGa                                                                                               >  1:94477/1‑149 (MQ=255)
                                                    tAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAAt                                                                                            <  2:3780050/149‑1 (MQ=255)
                                                         ttGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACt                                                                                       <  1:4419128/149‑1 (MQ=255)
                                                              cGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTAcc                                                                                  <  1:3621206/149‑1 (MQ=255)
                                                                tttGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTAACGTGCTGATGCATGGTATGCAGCTGGCGCTATTGACATCCACTTTACCGa                                                                                >  2:4486698/1‑149 (MQ=255)
                                                                  tGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGaaa                                                                              <  2:3991731/149‑1 (MQ=255)
                                                                    ttAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGa                                                                            >  2:4470216/1‑149 (MQ=255)
                                                                          ctctGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGccc                                                                      <  1:3845489/149‑1 (MQ=255)
                                                                             tGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTCTGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCa                                                                   >  2:814998/1‑149 (MQ=255)
                                                                             tGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCa                                                                   >  1:553447/1‑149 (MQ=255)
                                                                                 tttGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATg                                                               >  1:4173552/1‑149 (MQ=255)
                                                                                 tttGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATg                                                               >  1:3144970/1‑149 (MQ=255)
                                                                                 tttGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATg                                                               >  1:1005539/1‑149 (MQ=255)
                                                                                     gggATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGacca                                                           <  1:137326/149‑1 (MQ=255)
                                                                                      ggATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGaccac                                                          <  2:3572005/149‑1 (MQ=255)
                                                                                      ggATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGaccac                                                          >  2:558445/1‑149 (MQ=255)
                                                                                      ggATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGaccac                                                          <  2:4609888/149‑1 (MQ=255)
                                                                                      ggATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGaccac                                                          <  1:969781/149‑1 (MQ=255)
                                                                                       gATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATTGCCCGCAGATGaccacc                                                         >  1:3210552/1‑149 (MQ=255)
                                                                                       gATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGaccacc                                                         >  1:3785474/1‑149 (MQ=255)
                                                                                         tttttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCAccgc                                                       >  1:87381/1‑149 (MQ=255)
                                                                                          ttttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCAccgcc                                                      <  1:2594054/149‑1 (MQ=255)
                                                                                          ttttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCAccgcc                                                      <  1:234873/149‑1 (MQ=255)
                                                                                               aTCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGaaaa                                                 <  1:1422696/149‑1 (MQ=255)
                                                                                                     aaCCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACg                                           >  1:884377/1‑149 (MQ=255)
                                                                                                      aCCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACgg                                          >  1:1109431/1‑149 (MQ=255)
                                                                                                         tGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGAtt                                       <  2:3888340/149‑1 (MQ=255)
                                                                                                            tGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTc                                    >  1:4133735/1‑149 (MQ=255)
                                                                                                             gCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTcc                                   <  1:728173/149‑1 (MQ=255)
                                                                                                                tCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCttt                                >  2:1848205/1‑149 (MQ=255)
                                                                                                                tCCCTTACCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCttt                                <  2:3752384/149‑1 (MQ=255)
                                                                                                                 cccTTCCCACGCCCGCTTAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCtttt                               <  1:3481607/149‑1 (MQ=255)
                                                                                                                   cTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTTCTGATGCATGGTATGCAGCTGGCGCTATTGAAATACACTGTACCGAAAGATGGCCCGCATATGACCACCCCCGAAAAGGTACGGATTTTCCTTTTCg                             >  1:112521/1‑149 (MQ=255)
                                                                                                                   cTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCg                             >  1:1872388/1‑149 (MQ=255)
                                                                                                                   cTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACGTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCg                             >  1:2265196/1‑149 (MQ=255)
                                                                                                                    ttCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCgg                            >  2:3603954/1‑149 (MQ=255)
                                                                                                                    ttCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCgg                            <  2:1057988/149‑1 (MQ=255)
                                                                                                                        cACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTTCTGATGAATGGTATGCAGCTTGCGCTATTGAAATACACTTTACCGAAAGAAGGCCCGCAGATGACCACCCCCGAAAAGGTAAGGAATTTCCTTTTAGGCgtg                        >  1:1466015/1‑149 (MQ=255)
                                                                                                                        cACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCgtg                        >  1:2913511/1‑149 (MQ=255)
                                                                                                                        cACGCCCGCTCAAAAACTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCgtg                        >  2:1946831/1‑149 (MQ=255)
                                                                                                                          cGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGtt                      <  1:3298638/149‑1 (MQ=255)
                                                                                                                           gCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGttt                     <  1:940511/149‑1 (MQ=255)
                                                                                                                            cccGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTg                    <  1:59500/149‑1 (MQ=255)
                                                                                                                               gCTGAATATCTTCGTTAACGTGGCGCTGTTTTTTCCCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAAc                 <  2:1993765/149‑1 (MQ=255)
                                                                                                                               gCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAAc                 <  1:4372936/149‑1 (MQ=255)
                                                                                                                               gCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAAc                 <  2:4230896/149‑1 (MQ=255)
                                                                                                                                  gAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActgc              <  2:3494108/149‑1 (MQ=255)
                                                                                                                                  gAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActgc              >  2:2254758/1‑149 (MQ=255)
                                                                                                                                    atatCTTCGTTAAAGTGGCGCTGATTTTTCCCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActgctg            <  2:1864173/149‑1 (MQ=255)
                                                                                                                                     tatCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctgg           >  2:1444815/1‑149 (MQ=255)
                                                                                                                                     tatCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctgg           <  1:3715514/149‑1 (MQ=255)
                                                                                                                                     tatCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctgg           >  2:891538/1‑149 (MQ=255)
                                                                                                                                       tCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGCAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGcgggcc         >  2:4185248/1‑149 (MQ=255)
                                                                                                                                       tCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGACAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcc         >  2:3290467/1‑149 (MQ=255)
                                                                                                                                       tCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcc         <  2:3964754/149‑1 (MQ=255)
                                                                                                                                       tCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcc         <  1:824830/149‑1 (MQ=255)
                                                                                                                                            gTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGTCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCa    <  1:1542122/149‑1 (MQ=255)
                                                                                                                                             ttAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCag   <  2:3988860/149‑1 (MQ=255)
                                                                                                                                             ttAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAAACCACTTTACCGAAATATGGCCCGCAGATTACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCag   >  1:4120025/1‑149 (MQ=255)
                                                                                                                                              tAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCaga  >  2:1767222/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAGA  >  CP009273/3598295‑3598585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑