Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,638,926 A→T 8.6% Y61F (TAT→TTT)  rlmJ → 23S rRNA m(6)A2030 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,638,9260AT 8.6% 942.8 / 62.7 310Y61F (TAT→TTT) rlmJ23S rRNA m(6)A2030 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (157/124);  new base T (8/19);  total (166/144)
Fisher's exact test for biased strand distribution p-value = 1.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 318 total aligned reads displayed.

AACCACGCCGACGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCTGCTACTGCGTGA  >  CP009273/3638778‑3639073
                                                                                                                                                    |                                                                                                                                                   
aaCCACGCCGACGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAata                                                                                                                                                     <  1:1225997/149‑1 (MQ=255)
    aCGCCGACGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATAtctc                                                                                                                                                 >  2:1777123/1‑149 (MQ=255)
       ccGACGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGaa                                                                                                                                              <  1:4251364/149‑1 (MQ=255)
        cGACGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGACAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAg                                                                                                                                             <  2:276625/149‑1 (MQ=255)
        cGACGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAg                                                                                                                                             <  2:2670744/149‑1 (MQ=255)
        cGACGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTGATCAGTTAGGCAGCGCACAAGCCGCGCGTAACGGCGAATATCTCGAAg                                                                                                                                             >  1:476522/1‑149 (MQ=255)
         gACGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTATGCAGCGAACATTCCCAGCGTAACCGCGAATATCTCGAAgg                                                                                                                                            >  1:502151/1‑149 (MQ=255)
         gACGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAgg                                                                                                                                            <  1:1647516/149‑1 (MQ=255)
          aCGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAAACGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTATGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGc                                                                                                                                           >  1:1684954/1‑149 (MQ=255)
          aCGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAAACCGTTGTCTATATCGACACCCACGCCGGGGCCGGGCGTGGACAATTATGCAGCGAACAATCCGAGCCTACCGGCGAAAATCTCAAAAGc                                                                                                                                           >  1:3257529/1‑149 (MQ=255)
              ccTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGGTATCAGTTATGCAGCGCACAAGCCGAGCGTACCGGCGAAAATCTCCAAGGCAACg                                                                                                                                       >  1:3693324/1‑149 (MQ=255)
                       tACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCCGCGAACAAACCGAGCGTACCGGCGAAAATCTCGAAGGCAACGCCCCTAACt                                                                                                                              >  2:2829752/1‑149 (MQ=255)
                         ccGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTTTCAGTTAGGCAGCGAACAAGCCGAGCGTACCGGCGCATATCTCGAAGGCAACGCCCGGATCTgg                                                                                                                            >  1:1765960/1‑149 (MQ=255)
                           gTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGgca                                                                                                                          >  2:696019/1‑149 (MQ=255)
                            ttCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGgcag                                                                                                                         >  2:4291710/1‑149 (MQ=255)
                                gagCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCACGAAGGCATCGCCCGTGTCTTGCAGCAgg                                                                                                                     >  1:4234818/1‑149 (MQ=255)
                                 agCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTATGCAGCGAACAATCCGAGCGTACCGGCGAATATCTCGAAGGCAACGCCCGTATCTGGCAGCAGGa                                                                                                                    >  1:2337297/1‑149 (MQ=255)
                                 agCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGa                                                                                                                    >  2:1177598/1‑149 (MQ=255)
                                 agCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGa                                                                                                                    <  1:599204/149‑1 (MQ=255)
                                  gCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGAc                                                                                                                   >  2:1013751/1‑149 (MQ=255)
                                       atcatcGAGTCGCTGAAAGATAAAGATAAACCGTTTCTCTATCTCGACACCCACCCAGGGGCCGGGCGTTATAAGTTAGGCAGCGAACAAGCCGAGCGTAACCGCGAATATAGAGAATGCAACGCCCCTATCTGGCAGCAGGTCGAttt                                                                                                              >  1:1778744/1‑149 (MQ=255)
                                       atcatcGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGAttt                                                                                                              <  1:1355385/149‑1 (MQ=255)
                                       atcatcGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGAttt                                                                                                              <  1:1422567/149‑1 (MQ=255)
                                       atcatcGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGAttt                                                                                                              <  1:2849561/149‑1 (MQ=255)
                                         catcGAGTCGCTGAAAGAGAAAGATAAACCGTTTATCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCCGCGAATATCTCGAAGGCATCGCCCGTATCAGGCAGCAGGACGATTTGc                                                                                                            >  1:3083601/1‑149 (MQ=255)
                                           tcGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGccc                                                                                                          <  2:2605206/149‑1 (MQ=255)
                                           tcGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGccc                                                                                                          <  2:3446150/149‑1 (MQ=255)
                                           tcGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGccc                                                                                                          <  2:3954556/149‑1 (MQ=255)
                                           tcGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGccc                                                                                                          <  2:4536109/149‑1 (MQ=255)
                                           tcGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGccc                                                                                                          <  2:1402554/149‑1 (MQ=255)
                                             gAGTCGCTGAAAGAGAAAGATAAAACCTTTATCTATCTCGACACCCACGCAGGGGCCGGGCGTTATAAGTTAGGCAGCGAACAAGCCGAGCGTACCCGCGCATAAAACGAAGGCAAAGCCCCTCTATGGCAGCAGGACGATTTTCCCGc                                                                                                        >  1:511457/1‑149 (MQ=255)
                                               gTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAg                                                                                                      >  2:4631502/1‑149 (MQ=255)
                                               gTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAg                                                                                                      >  2:2901189/1‑149 (MQ=255)
                                                  gCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAAc                                                                                                   >  1:2957764/1‑149 (MQ=255)
                                                   cTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACt                                                                                                  >  2:2086733/1‑149 (MQ=255)
                                                    tGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTg                                                                                                 <  1:3924160/149‑1 (MQ=255)
                                                      aaagagaaagaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGa                                                                                               >  1:353810/1‑149 (MQ=255)
                                                      aaagagaaagaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGa                                                                                               <  2:526388/149‑1 (MQ=255)
                                                      aaagagaaagaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGa                                                                                               >  1:3847781/1‑149 (MQ=255)
                                                        agagaaagaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAgg                                                                                             >  1:1529812/1‑149 (MQ=255)
                                                         gagaaagaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGc                                                                                            <  2:3072723/149‑1 (MQ=255)
                                                          agaaagaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACCTGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCg                                                                                           >  2:816968/1‑149 (MQ=255)
                                                          agaaagaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCg                                                                                           >  1:3509241/1‑149 (MQ=255)
                                                           gaaagaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGt                                                                                          <  1:2880850/149‑1 (MQ=255)
                                                            aaagaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATTTCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTa                                                                                         <  1:595952/149‑1 (MQ=255)
                                                            aaagaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTa                                                                                         >  1:3437904/1‑149 (MQ=255)
                                                               gaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGTCAGCGAACATGCCGAGCGTACCGGCGAATTTCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACAt                                                                                      <  2:162478/149‑1 (MQ=255)
                                                               gaTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACAt                                                                                      >  2:3225398/1‑149 (MQ=255)
                                                                aTAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATc                                                                                     >  2:3752305/1‑149 (MQ=255)
                                                                   aaCCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAAt                                                                                  >  2:3882653/1‑149 (MQ=255)
                                                                   aaCCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAAt                                                                                  <  1:2025729/149‑1 (MQ=255)
                                                                   aaCCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAAt                                                                                  <  1:4065092/149‑1 (MQ=255)
                                                                     ccGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAAtgt                                                                                <  1:2914386/149‑1 (MQ=255)
                                                                      cGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATTTCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAAtgtg                                                                               <  2:1887365/149‑1 (MQ=255)
                                                                      cGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATTTCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAAtgtg                                                                               <  2:1714069/149‑1 (MQ=255)
                                                                       gTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTgg                                                                              >  2:3639132/1‑149 (MQ=255)
                                                                         ttCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTa                                                                            <  2:4434590/149‑1 (MQ=255)
                                                                            tctATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTaaaa                                                                         >  2:477975/1‑149 (MQ=255)
                                                                                 ctcGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACtt                                                                    <  2:2567895/149‑1 (MQ=255)
                                                                                 ctcGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACtt                                                                    >  2:2530192/1‑149 (MQ=255)
                                                                                 ctcGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACtt                                                                    >  2:4273771/1‑149 (MQ=255)
                                                                                   cGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGCATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCa                                                                  >  1:2422675/1‑149 (MQ=255)
                                                                                    gACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCaa                                                                 <  1:4553443/149‑1 (MQ=255)
                                                                                      cacCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAAcc                                                               >  1:1099617/1‑149 (MQ=255)
                                                                                        cccACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGt                                                             <  1:2737297/149‑1 (MQ=255)
                                                                                             gCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCgg                                                        <  1:4376582/149‑1 (MQ=255)
                                                                                              cAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGt                                                       <  1:2757652/149‑1 (MQ=255)
                                                                                               aGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTc                                                      >  2:4401354/1‑149 (MQ=255)
                                                                                                 gggCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAAAATCTCGAAGGCATCGCCCGTATCTTGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAg                                                    >  1:3822914/1‑149 (MQ=255)
                                                                                                  ggCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGt                                                   <  2:2238671/149‑1 (MQ=255)
                                                                                                        gCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATTTCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGtt                                             <  2:3726228/149‑1 (MQ=255)
                                                                                                           ttATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTtact                                          >  1:1733536/1‑149 (MQ=255)
                                                                                                             aTCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTtactac                                        >  1:2500177/1‑149 (MQ=255)
                                                                                                               cAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTAccc                                      <  1:541274/149‑1 (MQ=255)
                                                                                                                 gTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCg                                    <  1:4603916/149‑1 (MQ=255)
                                                                                                                  ttAGGCAGCGAACATGCCGAGCGTACCGGCGAATTTCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCgg                                   <  1:194687/149‑1 (MQ=255)
                                                                                                                  ttAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCgg                                   >  2:1644881/1‑149 (MQ=255)
                                                                                                                    aGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGtt                                 <  1:2096629/149‑1 (MQ=255)
                                                                                                                    aGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGtt                                 >  1:2268334/1‑149 (MQ=255)
                                                                                                                     ggCAGCGAACATGCCGAGCGTACCGGCGAATTTCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTc                                <  2:2314312/149‑1 (MQ=255)
                                                                                                                     ggCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTc                                <  1:4654780/149‑1 (MQ=255)
                                                                                                                       cAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGc                              <  1:3478250/149‑1 (MQ=255)
                                                                                                                         gCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCg                            >  1:4188031/1‑149 (MQ=255)
                                                                                                                             aCATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGa                        >  2:4598615/1‑149 (MQ=255)
                                                                                                                              cATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGAt                       >  1:4291054/1‑149 (MQ=255)
                                                                                                                              cATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGAt                       <  1:947057/149‑1 (MQ=255)
                                                                                                                               aTGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGAtt                      <  2:1475330/149‑1 (MQ=255)
                                                                                                                               aTGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGAtt                      <  1:2683129/149‑1 (MQ=255)
                                                                                                                               aTGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGAtt                      <  2:3369487/149‑1 (MQ=255)
                                                                                                                                    gAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCg                 >  2:598281/1‑149 (MQ=255)
                                                                                                                                       cGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCt              <  1:1237823/149‑1 (MQ=255)
                                                                                                                                        gTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCTg             <  2:34860/149‑1 (MQ=255)
                                                                                                                                        gTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCTg             >  1:4161662/1‑149 (MQ=255)
                                                                                                                                        gTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCTg             >  2:926263/1‑149 (MQ=255)
                                                                                                                                           ccGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCTGCTa          <  1:3646691/149‑1 (MQ=255)
                                                                                                                                             ggCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCTGCTACt        <  1:2655697/149‑1 (MQ=255)
                                                                                                                                                 aaTATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCTGCTACTGCGt    <  1:1040529/149‑1 (MQ=255)
                                                                                                                                                  atatCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGCACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCTGCTACTGCGTg   >  1:638763/1‑149 (MQ=255)
                                                                                                                                                   tatCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCTGCTACTGCGTGa  >  2:4081526/1‑149 (MQ=255)
                                                                                                                                                   tatCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCTGCTACTGCGTGa  >  1:3299989/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AACCACGCCGACGTCCTTAAACATACCGTTCAGAGCCTGATCATCGAGTCGCTGAAAGAGAAAGATAAACCGTTTCTCTATCTCGACACCCACGCAGGGGCCGGGCGTTATCAGTTAGGCAGCGAACATGCCGAGCGTACCGGCGAATATCTCGAAGGCATCGCCCGTATCTGGCAGCAGGACGATTTGCCCGCAGAACTGGAGGCGTACATCAATGTGGTAAAACACTTCAACCGTAGCGGTCAGTTGCGTTACTACCCCGGTTCGCCGTTGATTGCTCGCCTGCTACTGCGTGA  >  CP009273/3638778‑3639073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑