| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,652,308 | G→T | 10.4% | intergenic (+55/‑284) | gadE → / → mdtE | gad regulon transcriptional activator/anaerobic multidrug efflux transporter, ArcA‑regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,652,308 | 0 | G | T | 10.4% | 915.0 / 32.3 | 298 | intergenic (+55/‑284) | gadE/mdtE | gad regulon transcriptional activator/anaerobic multidrug efflux transporter, ArcA‑regulated |
| Reads supporting (aligned to +/- strand): ref base G (165/90); new base T (10/33); total (175/123) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 300 total aligned reads displayed.
AACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCAC > CP009273/3652160‑3652456 | aacaTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTg > 1:193767/1‑149 (MQ=255) aTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAtt < 2:678678/149‑1 (MQ=255) aTTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAtt > 1:4584923/1‑149 (MQ=255) ttAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAttt > 1:101255/1‑149 (MQ=255) ttAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGAttt > 1:1980282/1‑149 (MQ=255) aaaGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCa > 1:231206/1‑149 (MQ=255) aaGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAt > 1:1527919/1‑149 (MQ=255) gATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑a > 1:438188/1‑149 (MQ=255) gATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑a > 2:3232149/1‑149 (MQ=255) aGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑Accccc < 1:4090396/149‑1 (MQ=255) gAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCg > 2:3824657/1‑149 (MQ=255) aaTTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGt > 2:2006250/1‑149 (MQ=255) ttCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTTATTTCAT‑T‑ACCCCCGGTGa < 1:3697608/149‑1 (MQ=255) cAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGAtt < 1:778133/149‑1 (MQ=255) cAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGAtt < 1:1275605/149‑1 (MQ=255) aaCATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTa > 1:88008/1‑149 (MQ=255) aTTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTa < 1:4427023/149‑1 (MQ=255) aTTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACACCCGGTGATTACTa > 2:3870322/1‑149 (MQ=255) tATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTaaa < 2:3848587/149‑1 (MQ=255) tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAgg < 2:3115446/149‑1 (MQ=255) tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAgg > 2:2655691/1‑149 (MQ=255) tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAgg > 2:2357253/1‑149 (MQ=255) tGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAgg > 1:3825429/1‑149 (MQ=255) tGTACAAGATGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCAAATCTTTTTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGTCATGTCGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTTCTAAAgg < 2:783654/149‑1 (MQ=255) gTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGTTGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGga < 2:1921634/149‑1 (MQ=255) tACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGgag > 2:2984809/1‑149 (MQ=255) tACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGgag > 2:594995/1‑149 (MQ=255) aCAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTTATTTCAT‑T‑ACCCCCGGTGATTACTAAAGgaga > 2:2509147/1‑149 (MQ=255) aCAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGgaga < 2:3895329/149‑1 (MQ=255) aCAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGgaga < 1:4214432/149‑1 (MQ=255) cAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGagag > 2:1648549/1‑149 (MQ=255) cAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGagag > 2:4036074/1‑149 (MQ=255) aGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGc > 1:250912/1‑149 (MQ=255) gCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCt < 1:3235965/149‑1 (MQ=255) aaaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTaaaa < 1:4507676/149‑1 (MQ=255) aaCTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAAc > 2:4438103/1‑149 (MQ=255) cTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGa < 1:932649/149‑1 (MQ=255) aaGACGTATGAGCGAAATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTTAAATTTATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTAATAAAGGAGAGGCTAAAACGACt < 1:1549128/149‑1 (MQ=255) aTGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGAAATAAGCAACTGAAATTTATGGCTGGCATGACGTGGGATGCAGATGCTTATTTCTT‑T‑ACCCCCGGTGATTACTAAAGGAGTGGCTAAAACGACTTTATTcc < 2:3378098/149‑1 (MQ=255) tGAGCGACATCTTCACCCTGGGTATCACATCTTATTTTTAGTCAGTACATAAGAAACTGAATTTGATGGCTGGCTTGACGAGGGATGCTGTTGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc < 2:3053324/149‑1 (MQ=255) tGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTccc > 2:2843612/1‑149 (MQ=255) gACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGt > 2:3597714/1‑149 (MQ=255) aCATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTTATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTa > 1:772514/1‑149 (MQ=255) cATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTAt > 2:4024915/1‑149 (MQ=255) cATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTAt > 2:4268447/1‑149 (MQ=255) cggggTATCACATCTTATTTTTAGTCCGGACATAAGCAACTGAAATTGAGGGCTGGCATGACGTGGGAGGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAACGGAGCGGATAACACGACTTTATTCCCCTGGTATGTGTATccac < 2:10259/147‑1 (MQ=255) tGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATccacc > 1:176641/1‑149 (MQ=255) gggTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATccacca > 1:4769383/1‑149 (MQ=255) ggTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAg > 2:1087072/1‑149 (MQ=255) ggTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAg > 1:3869643/1‑149 (MQ=255) tATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTa > 2:3363114/1‑149 (MQ=255) tATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTa > 2:1381524/1‑149 (MQ=255) tCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGa > 1:2892288/1‑149 (MQ=255) acaTCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAc > 2:3444796/1‑149 (MQ=255) caTCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAcc > 2:2770440/1‑149 (MQ=255) caTATTTTTTTTCTTCAGTAAATAAGCAACTTAATTTGTTGGCTGGCTTGACTTGGGAGGCATTTGGTTTTTTATT‑T‑ACCCCCGGTTATTACTAACGGAGTGGCTAACACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAcc < 2:4402267/149‑1 (MQ=255) caTATTTTTTTTAGTCTGTAAATAAGAAACTGAATTTGTGGGCTGGATTGACGTGGGTTGCAGTTGCTTTTTTCTT‑T‑ACCCCGGGTGATTAATAAAGGAGAGGCTAACACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAcc < 1:3449999/149‑1 (MQ=255) aTCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAccc > 2:1429106/1‑149 (MQ=255) aTCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAAccc > 1:1751135/1‑149 (MQ=255) tCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCt > 1:3928006/1‑149 (MQ=255) cTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCtt > 1:3983394/1‑149 (MQ=255) ttATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTTATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTc > 2:1623545/1‑149 (MQ=255) ttATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTc > 2:3527657/1‑149 (MQ=255) ttATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTc > 2:859214/1‑149 (MQ=255) ttATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTc > 1:2077748/1‑149 (MQ=255) aTTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGt > 2:1170347/1‑149 (MQ=255) tttAGTCAGGACATAAGAAACTGATTTTGTTGGGGGTCATGAGGTGGGATGCATTTGCTTATTTCAT‑T‑CCCCCGGTTGATTAATACCGGAGCGGATAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGc < 1:981941/149‑1 (MQ=255) gTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGa > 2:19061/1‑149 (MQ=255) gTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGa > 1:4074437/1‑149 (MQ=255) tCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGaa > 2:3950994/1‑149 (MQ=255) cAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAAt > 1:603536/1‑149 (MQ=255) aCATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTg > 1:3724950/1‑149 (MQ=255) tAAGAAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTTTTTTCAT‑T‑ACCCCCGGTGATTAATAACGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCa < 1:2670518/149‑1 (MQ=255) acaaaCTTAAATTGTGGGCTGGCATTCCGTGGGTTGCTGTTGGTGTTTTCAT‑T‑CCCCCGGGTTATTAATACCGGAGAGTAAAACACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAgg < 1:809181/146‑1 (MQ=255) gCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGa > 2:1275624/1‑149 (MQ=255) ccAACTTAAATTGGGGGCGGGCATGAGGTGGTTTGCTGTTGCTTTTTTCAT‑T‑CCCCCCGGTTATTACTAACGGAGCGCATAACACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGa < 1:2418096/148‑1 (MQ=255) cAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGTTGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGaa < 2:185423/149‑1 (MQ=255) cAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTTATTTCAT‑T‑CCCCCCGTTGATTACTAACGTAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGaa < 1:3524820/149‑1 (MQ=255) aaCTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAAc > 1:1617279/1‑149 (MQ=255) ttaatttGAGGGCTGGCATGACGTGGTATGCTGTTGCTGTTTTCAT‑T‑ACCCCCGGTGTTTAATAAAGGCGAGGATAACACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGt < 2:4474668/144‑1 (MQ=255) tGAAATTGTTGGCTGGCATGACGTGGGATGCAGTTGCTGATTTCAT‑T‑ACCCCGGGTGATTACTAACGGAGTGGCTAACACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGt < 1:1561420/149‑1 (MQ=255) tGAAATTGATGGCGGGCATGACGTGGGATGCAGATGCTTATTTAAT‑T‑ACCCCCGGTTTTTACTAAAGTAGAGGATAACACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGt < 1:1070636/149‑1 (MQ=255) gAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGtt > 1:2135682/1‑149 (MQ=255) aaTTGATGGCTGGCATGACGAGGGATGCAGATGCTTATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTgg < 2:4308450/149‑1 (MQ=255) ttGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCa > 2:2901758/1‑149 (MQ=255) ttagggctggcATGACGTGGTATGCAGATGCTGATTTCAT‑TCCCCCCCGGTTATTACTAACGGAGAGCCAAACACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCa < 1:543771/145‑1 (MQ=255) tGATGGCTGGCAGGACGTGGGATGCAGCTGCTGATTTCATAC‑CCCCCCGGTTATTACTAACGGAGAGGCTAACACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCa < 1:1458642/149‑1 (MQ=255) tggctggcTTGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCGGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAAc < 2:488067/149‑1 (MQ=255) tggcgggcATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTAATAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAAc < 2:1524001/149‑1 (MQ=255) ggcATGACGTGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAACGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCaa < 2:4305894/149‑1 (MQ=255) tGACGTGGGATGCTGATGCTGATTTCAT‑T‑CCCCCGGGTGATTACTAACGGAGCGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACAtt < 1:1195248/149‑1 (MQ=255) aCGTGGGAGGCAGATGCTGATTTCAT‑T‑ACCCCCGGTTATTACTAACGGCGCGGCTAACACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACAtttt < 1:235708/149‑1 (MQ=255) aCGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACAtttt > 2:1573210/1‑149 (MQ=255) cgtgggATGCAGATGCTGTTTTAAT‑T‑ACCCCCGGTGATTACTAACGGAGCGGATAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACAttttt < 1:3935084/146‑1 (MQ=255) ggttGCTGATGCTTTTTTCAT‑T‑ACCCCGGGTGATTACTAACGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTg < 2:2400574/146‑1 (MQ=255) gATGCAGATGGTGATTTCAT‑T‑ACCCCCGTTGATTACTAAAGTAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGt < 2:1770165/149‑1 (MQ=255) ggCAGTTGCTGATTTCAT‑C‑CCCCCCGGTGATTCCTACCGGAGTGGCTACCACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCg < 1:2161652/148‑1 (MQ=255) cAGTTGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAACGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGtt < 2:1970759/149‑1 (MQ=255) gATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGa > 2:2036750/1‑149 (MQ=255) tGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGAcc < 2:4138138/149‑1 (MQ=255) | AACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAACTAAGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAGTCAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAT‑T‑ACCCCCGGTGATTACTAAAGGAGAGGCTAAAACGACTTTATTCCCCTGGTATGTGTATCCACCAGTAGAACCCTTCGTTGCCCGAATGCTGGCAGGAACTGTTGGCAGAACGGCAACATTTTTTTTGTCGTTGACCTCAC > CP009273/3652160‑3652456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |