Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,698,875 G→T 100% F92L (TTC→TTA)  dppB ← dipeptide/heme transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,698,8750GT98.7% 1092.8 / 3.0 323F92L (TTC→TTA) dppBdipeptide/heme transporter
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base T (153/165);  total (157/166)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 323 total aligned reads displayed.

TAACCTGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAG  >  CP009273/3698729‑3699019
                                                                                                                                                  |                                                                                                                                                
tAACCTGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTaa                                                                                                                                                >  2:572966/1‑149 (MQ=255)
  aCCTGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAAct                                                                                                                                              >  1:1693945/1‑149 (MQ=255)
   ccTGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAActc                                                                                                                                             >  2:1092200/1‑149 (MQ=255)
    cTGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAActct                                                                                                                                            >  2:4487938/1‑149 (MQ=255)
     tGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCtt                                                                                                                                           >  1:811282/1‑149 (MQ=255)
     tGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCtt                                                                                                                                           >  2:221786/1‑149 (MQ=255)
      gTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTc                                                                                                                                          <  2:2664832/149‑1 (MQ=255)
       tCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTcc                                                                                                                                         <  1:3252584/149‑1 (MQ=255)
       tCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTcc                                                                                                                                         <  1:1415784/149‑1 (MQ=255)
          gcgcCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCaa                                                                                                                                      >  1:2820342/1‑149 (MQ=255)
             ccAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAAcc                                                                                                                                   >  2:2539617/1‑149 (MQ=255)
             ccAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAAcc                                                                                                                                   >  1:3783898/1‑149 (MQ=255)
              cAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCg                                                                                                                                  <  1:2274645/149‑1 (MQ=255)
               aGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCgg                                                                                                                                 >  1:434814/1‑149 (MQ=255)
                ggCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCggg                                                                                                                                >  1:4488267/1‑149 (MQ=255)
                  ccAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGAt                                                                                                                              <  1:1466111/149‑1 (MQ=255)
                    aaCCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGc                                                                                                                            >  2:1776068/1‑149 (MQ=255)
                       cGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGc                                                                                                                         <  2:1325747/149‑1 (MQ=255)
                        gCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCt                                                                                                                        <  1:1968882/149‑1 (MQ=255)
                        gCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCt                                                                                                                        >  2:3469726/1‑149 (MQ=255)
                        gCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCt                                                                                                                        >  2:3661884/1‑149 (MQ=255)
                           gtgtgATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCtttt                                                                                                                     <  2:3235811/149‑1 (MQ=255)
                           gtgtgATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCtttt                                                                                                                     <  1:4786523/149‑1 (MQ=255)
                             gtgATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCa                                                                                                                   >  2:312749/1‑149 (MQ=255)
                              tgATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCAt                                                                                                                  <  2:857470/149‑1 (MQ=255)
                               gATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCAtt                                                                                                                 >  2:3389616/1‑149 (MQ=255)
                                    aaaaTGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAAt                                                                                                            <  2:4262077/149‑1 (MQ=255)
                                     aaaTGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATg                                                                                                           >  1:4619684/1‑149 (MQ=255)
                                       aTGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGcc                                                                                                         <  2:1360336/149‑1 (MQ=255)
                                        tGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCt                                                                                                        <  1:3681249/149‑1 (MQ=255)
                                        tGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCt                                                                                                        >  1:3853712/1‑149 (MQ=255)
                                            aCCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGAt                                                                                                    >  1:3079823/1‑149 (MQ=255)
                                            aCCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGAt                                                                                                    <  1:1287871/149‑1 (MQ=255)
                                             ccGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATc                                                                                                   >  2:4116915/1‑149 (MQ=255)
                                                  tttAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCAt                                                                                              <  2:4380253/149‑1 (MQ=255)
                                                      aCCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCAt                                                                                          >  2:1631307/1‑149 (MQ=255)
                                                      aCCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCGTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCAt                                                                                          <  2:4319591/149‑1 (MQ=255)
                                                         gCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAAc                                                                                       >  1:1133060/1‑149 (MQ=255)
                                                            gccagcACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGcc                                                                                    >  1:4581762/1‑149 (MQ=255)
                                                             ccagcACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTACCTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGccc                                                                                   >  1:3099430/1‑149 (MQ=255)
                                                             ccagcACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGccc                                                                                   <  1:2879854/149‑1 (MQ=255)
                                                             ccagcACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTAATTGAAATGCCTAGATCGCCATGCATAACGccc                                                                                   >  1:1871536/1‑149 (MQ=255)
                                                              cagcACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGcccc                                                                                  >  1:645478/1‑149 (MQ=255)
                                                                gcACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCaa                                                                                >  1:2771075/1‑149 (MQ=255)
                                                                gcACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCaa                                                                                >  1:3421988/1‑149 (MQ=255)
                                                                     ccGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTa                                                                           <  2:669800/149‑1 (MQ=255)
                                                                     ccGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGAGTGGACTGGAAGCGCGGCACTAACTCTTCCCATACCGGTATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTa                                                                           <  2:1960599/149‑1 (MQ=255)
                                                                         ccGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATgg                                                                       <  1:1003522/149‑1 (MQ=255)
                                                                         ccGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATgg                                                                       <  1:2397505/149‑1 (MQ=255)
                                                                           ggAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGgag                                                                     >  2:4418507/1‑149 (MQ=255)
                                                                           ggAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGgag                                                                     >  2:4555339/1‑149 (MQ=255)
                                                                           ggAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGgag                                                                     >  1:2171906/1‑149 (MQ=255)
                                                                            gAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGgaga                                                                    >  1:3755353/1‑149 (MQ=255)
                                                                               tACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATAc                                                                 <  1:4248881/149‑1 (MQ=255)
                                                                               tACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATAc                                                                 <  1:1656261/149‑1 (MQ=255)
                                                                               tACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATAc                                                                 >  1:845031/1‑149 (MQ=255)
                                                                                aCCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATAct                                                                >  2:2092124/1‑149 (MQ=255)
                                                                                 ccGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATActg                                                               >  2:4058540/1‑149 (MQ=255)
                                                                                 ccGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATActg                                                               >  2:237140/1‑149 (MQ=255)
                                                                                  cGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATActgc                                                              <  2:3566889/149‑1 (MQ=255)
                                                                                   gACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCACACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATActgct                                                             <  2:793495/149‑1 (MQ=255)
                                                                                   gACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATActgct                                                             <  2:286092/149‑1 (MQ=255)
                                                                                   gACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATActgct                                                             >  2:3832821/1‑149 (MQ=255)
                                                                                   gACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATActgct                                                             <  1:2872763/149‑1 (MQ=255)
                                                                                     cTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACtgctgc                                                           <  1:3000920/149‑1 (MQ=255)
                                                                                     cTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACtgctgc                                                           >  2:4779768/1‑149 (MQ=255)
                                                                                        ccGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCcac                                                        <  1:3505747/149‑1 (MQ=255)
                                                                                          gTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACAt                                                      >  1:2691778/1‑149 (MQ=255)
                                                                                            aGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCg                                                    >  1:4133128/1‑149 (MQ=255)
                                                                                            aGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCg                                                    >  1:1153506/1‑149 (MQ=255)
                                                                                            aGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGCGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATCGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCg                                                    <  1:2310423/149‑1 (MQ=255)
                                                                                              cAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGt                                                  <  2:1483010/149‑1 (MQ=255)
                                                                                               aaaaaTCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGtt                                                 <  1:1092200/149‑1 (MQ=255)
                                                                                               aaaaaTCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGtt                                                 >  1:2258263/1‑149 (MQ=255)
                                                                                                 aaaTCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTa                                               <  2:2885102/149‑1 (MQ=255)
                                                                                                   atcatcGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATc                                             <  1:3091809/149‑1 (MQ=255)
                                                                                                   atcatcGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATc                                             >  2:276671/1‑149 (MQ=255)
                                                                                                    tcatcGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCt                                            >  2:1030821/1‑149 (MQ=255)
                                                                                                       tcGCGCAGAGGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAg                                         >  1:159976/1‑149 (MQ=255)
                                                                                                        cgcgCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGc                                        <  1:4200655/149‑1 (MQ=255)
                                                                                                         gcgcAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGcc                                       <  2:3883303/149‑1 (MQ=255)
                                                                                                         gcgcAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGcc                                       <  1:2058566/149‑1 (MQ=255)
                                                                                                            cAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAg                                    <  2:19156/149‑1 (MQ=255)
                                                                                                             aGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGt                                   >  2:248992/1‑149 (MQ=255)
                                                                                                             aGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGt                                   >  1:441317/1‑149 (MQ=255)
                                                                                                              gACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGtt                                  <  1:2530146/149‑1 (MQ=255)
                                                                                                                cGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTca                                <  1:3539822/149‑1 (MQ=255)
                                                                                                                    aaGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTcagcca                            <  2:1700243/149‑1 (MQ=255)
                                                                                                                            gCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTgc                    >  1:4072428/1‑149 (MQ=255)
                                                                                                                            gCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATATAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTgc                    <  2:181396/149‑1 (MQ=255)
                                                                                                                               tGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGcgcg                 >  1:3830144/1‑149 (MQ=255)
                                                                                                                                    tGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACg            >  2:3307928/1‑149 (MQ=255)
                                                                                                                                     ggAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGc           >  1:2476621/1‑149 (MQ=255)
                                                                                                                                      gAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAACTGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGct          <  1:4680534/149‑1 (MQ=255)
                                                                                                                                        aGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGctct        >  2:4077934/1‑149 (MQ=255)
                                                                                                                                        aGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGctct        <  1:4469389/149‑1 (MQ=255)
                                                                                                                                        aGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGctct        >  1:1929614/1‑149 (MQ=255)
                                                                                                                                        aGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGctct        <  1:1032690/149‑1 (MQ=255)
                                                                                                                                         gcgcGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTg       >  2:717773/1‑149 (MQ=255)
                                                                                                                                              gCACTAACTCTTCCCCAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGgag  <  2:1272533/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TAACCTGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAG  >  CP009273/3698729‑3699019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑