Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,469 A→C 6.9% D421E (GAT→GAG)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4690AC 6.9% 603.3 / 4.8 285D421E (GAT→GAG) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base A (88/165);  new base C (24/5);  total (114/171)
Fisher's exact test for biased strand distribution p-value = 8.83e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

TTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCG  >  CP009273/3721321‑3721617
                                                                                                                                                    |                                                                                                                                                      
tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCcaca                                                                                                                                                        >  2:3952889/1‑149 (MQ=255)
 ttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAt                                                                                                                                                       <  2:1596966/149‑1 (MQ=255)
  tctcGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAtg                                                                                                                                                      <  2:4567048/149‑2 (MQ=255)
    tcgtcgTGGCTGATTAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccc                                                                                                                                                   <  2:2761332/149‑1 (MQ=255)
      gtcgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAGTTCTCTGTATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCGGGCCCCACAT‑cccc‑c                                                                                                                                                >  2:3994238/1‑149 (MQ=255)
          tGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCg                                                                                                                                            >  2:1231113/1‑149 (MQ=255)
            gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCCT‑CCCC‑CCCCGtg                                                                                                                                          >  1:2804103/1‑148 (MQ=255)
               gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACTT‑CCCC‑CCCCGTACgg                                                                                                                                       >  1:1057345/1‑149 (MQ=255)
                  aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTaa                                                                                                                                    <  2:725342/149‑1 (MQ=255)
                   aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGGGCGGttgg                                                                                                                                   >  1:2193953/1‑146 (MQ=255)
                   aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCGC‑CCCCGTGCGGtggt                                                                                                                                   >  2:3782062/1‑146 (MQ=255)
                   aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGGACgggggt                                                                                                                                   >  2:1548978/1‑145 (MQ=255)
                    gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACggggatc                                                                                                                                  >  2:97450/1‑144 (MQ=255)
                      ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGGACGGTCCTCGa                                                                                                                                >  1:2403736/1‑149 (MQ=255)
                       gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCCGTGCTGGCCCCACAG‑CCCC‑CCCCGTACGGTCATCgcg                                                                                                                               >  2:1887466/1‑147 (MQ=255)
                       gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTACGGTAATCGAg                                                                                                                               >  1:4744282/1‑149 (MQ=255)
                        cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAgc                                                                                                                              <  2:1621201/149‑1 (MQ=255)
                         aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTGCGGTAATCGAgcg                                                                                                                             >  2:806370/1‑148 (MQ=255)
                                tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑CCCC‑CCCCGTACGGTCATCGAGCTGCTGTcc                                                                                                                      >  1:4007810/1‑149 (MQ=255)
                                 gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTCCGGGCATCGGGCGGCTGACCg                                                                                                                     >  1:3146452/1‑149 (MQ=255)
                                   gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                   <  2:1035515/149‑1 (MQ=255)
                                     aTCTGGTAGAGGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGa                                                                                                                 <  2:3324115/149‑1 (MQ=255)
                                        tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                                              <  1:2628458/149‑1 (MQ=255)
                                          gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                            <  2:2337872/149‑1 (MQ=255)
                                          gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                            <  2:3153006/149‑1 (MQ=255)
                                           tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTCCGGGGGGGGAGCTGCTGTCCGGTGGGATCCg                                                                                                           >  2:452270/1‑149 (MQ=255)
                                              aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTCCGGTACGCGAGCTGCTGACCGCGGGGAGCCGCCa                                                                                                        >  2:4259077/1‑149 (MQ=255)
                                              aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                        <  2:1477142/149‑1 (MQ=255)
                                               tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTCCGGGAATCGAGCTGCTGACCGCTGATATCCGGCAg                                                                                                       >  2:3519642/1‑149 (MQ=255)
                                                  gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                    <  1:2304313/149‑1 (MQ=255)
                                                   aCTGTTCTACCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCGTGCTGCGCCCAGTGCTGGCCCCCCAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                   <  2:3400391/149‑1 (MQ=255)
                                                   aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGGGCGGTCATCGAGCTGCTGTCCGCTGATGTCCGCCAGcggc                                                                                                   >  2:2530063/1‑146 (MQ=255)
                                                    cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGCTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                  <  1:1983296/149‑1 (MQ=255)
                                                      gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                <  1:4382207/149‑1 (MQ=255)
                                                      gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCGGTCCGCGGATATCCGCCAGCATCTGa                                                                                                >  1:3972559/1‑149 (MQ=255)
                                                       ttCTAACGGGAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                               <  2:4091647/149‑1 (MQ=255)
                                                             cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                                         <  2:3169873/149‑1 (MQ=255)
                                                                tAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                                      <  1:1632924/149‑1 (MQ=255)
                                                                  gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGGTGGGGAATCGGGGTGCTGCCCGCTGCTATCCGCCAGCATCTGACGCCGGGACtga                                                                                    >  2:2305515/1‑147 (MQ=255)
                                                                   ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                   <  1:3851306/149‑1 (MQ=255)
                                                                   ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                   <  2:3285572/149‑1 (MQ=255)
                                                                    tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                  <  1:1193889/149‑1 (MQ=255)
                                                                       ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGGCCGCGGATGTCCGCCCGCATCGGGCGCCCGTACTCGCTAcg                                                                               >  1:1234040/1‑149 (MQ=255)
                                                                        gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgc                                                                              <  2:56407/149‑1 (MQ=255)
                                                                         caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTACGGGAATCGTGCTGCTGGCCGGGGATGTCCGCCAGCATCGGACGCCAGTCCTCAGTAcgcg                                                                             >  1:549442/1‑149 (MQ=255)
                                                                          aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACgcgc                                                                            <  2:21378/149‑1 (MQ=255)
                                                                           acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGGGCGGTCAGCGGGCTGCGGGCCGCGGGTAGCCGCCGGCCTCTGACGCCCGTACGCACGACGcgcc                                                                           >  1:146133/1‑149 (MQ=255)
                                                                            caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                                          <  1:2504974/149‑1 (MQ=255)
                                                                              aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTGCGGGAATCGGGCTGCTGAGCGGTGATATCCGCCCGCCTCTGACGCCAGTCCTCACGACGcgccccg                                                                        >  2:2830462/1‑149 (MQ=255)
                                                                               ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTACGGTACTCGAGCTGCTGACCGCTGATTGCCGCCAGCCGCTGACGCCAGTTCTCACTCCGcgccccgc                                                                       >  1:2651163/1‑149 (MQ=255)
                                                                                   aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTCCGGTCCGCGAGCTGCTGGCCGCGGGGATCCGCCCGCGTCTGGCGCCCGGCCTCGCTCCGCGCCCCGgcccc                                                                   >  2:1403965/1‑149 (MQ=255)
                                                                                    tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGGAATCGAGCTGCGGACCGCTGTTATCCGCCAGCCTCTGACGCCCGTACTCACTACGCGCCCCGcccccc                                                                  >  1:2686599/1‑148 (MQ=255)
                                                                                     gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTTCTGGACCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGTTGATATCCGCCAGCATCTGACGCCAGTAATCACTACGCGCCCCGCCCCCaa                                                                 <  2:1929440/149‑1 (MQ=255)
                                                                                     gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTGCGGGAATGGGGCGGCTGTCCGGTGTTGGCCGCCCGCATGTGTGGGCAGTTCTGACGACGCGGCCCGgccccca                                                                 >  2:840152/1‑147 (MQ=255)
                                                                                     gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATGCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCGCCCac                                                                 >  1:4661051/1‑148 (MQ=255)
                                                                                     gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                 <  1:877139/149‑1 (MQ=255)
                                                                                     gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                 <  2:4774532/149‑1 (MQ=255)
                                                                                     gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGGAATCGAGCTGCTGACCGCTGTTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGcccccca                                                                 >  2:1515477/1‑147 (MQ=255)
                                                                                     gAGCGATTTCTCGGGATGCGCCGCGATCAGCGCCAGCCTGGCTGCGCCCAGTGCTGGCCCCACAG‑CCCC‑CCCCGTACGGTAATCGACCTGCTGACCGCTGATATCCGCCATCATCTGACGCCAGTACTCACTCCGCGCCCCGCCCCCaa                                                                 <  2:4624082/149‑1 (MQ=255)
                                                                                      aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGGCCGGGAATCGAGCTGCTGGCCGCGGTTTTCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGcccccgcg                                                                >  1:3525897/1‑146 (MQ=255)
                                                                                            ttCTCTGGTTTCGCCGCGATCTGCGCCAGCCTTGCTGCGGCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATGGGCGAGCATCTGACGACAGTACTCACTACGCGCCCCGCGAACATTCAACGt                                                          <  2:3881829/149‑1 (MQ=255)
                                                                                            ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                          <  1:3043481/149‑1 (MQ=255)
                                                                                            ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                          <  1:2403623/149‑1 (MQ=255)
                                                                                            ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                                         <  2:2645914/149‑1 (MQ=255)
                                                                                               tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTTCGGTACTCGTGCTGCTGTCCGCTGGTTTCCGCCAGCATCTGACGCCAGTACTCACTGCGCGCCCCGCCCCCCCTCAACGTCac                                                       >  1:4445882/1‑149 (MQ=255)
                                                                                                 tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAacac                                                     <  1:567938/149‑1 (MQ=255)
                                                                                                  ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTGATCGGGCTGGGGTCGGCGGGGGTGCGCCAGCAGCTGCCGCCGGTCCTCACTACGCGCCCCGCCCCCCAGCAACGGAACGCt                                                    >  1:1386925/1‑149 (MQ=255)
                                                                                                   gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGGCCGGTACGCGCGCGGCTGCCCGCTGATATCCGCCCGCATCTGACGCCAGGACGCCCTACGCGCCCCGCCCGCCAGCAACGGAgcacgg                                                   >  2:3772492/1‑147 (MQ=255)
                                                                                                   gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCC‑CCCCGTGCGGTAATCGCGCTGCTGACCGCGGATATCCGCCCGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAacacgt                                                   >  1:1100025/1‑147 (MQ=255)
                                                                                                     ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTGCGGGAATCGAGCTGCTGACCGCGGTTGTCCGCCAGCATCTGACGCCCGGACTCACTACGCGCCCCGCCCCCCATCAACGTAACCCTTTg                                                 >  1:2328812/1‑149 (MQ=255)
                                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTCCGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGTCGCCCGTACTCACTACGCGCCCCGCCCCCGCGCAACGGACCCCTTTGCgg                                              >  2:4350757/1‑149 (MQ=255)
                                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCGCCCCGTGCGGGGAGCGAGCTGCGGCCCGCGGTGGTCCGCCCGCATCTGTCGCCGGGACGGACGGCGCGGGCCGCGCGCAAGCCACGTTACACTTGGCg                                               >  2:754050/1‑149 (MQ=255)
                                                                                                         ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCGGTCCGCTGATAGCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCggg                                             >  1:3151781/1‑148 (MQ=255)
                                                                                                          cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTGCGGTAATCGGGCTGGTGACGGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCATTCAACGGCGCACTTTGCGGtt                                            >  1:631500/1‑149 (MQ=255)
                                                                                                          cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                            <  1:1515356/149‑1 (MQ=255)
                                                                                                           gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                           >  2:3009715/1‑149 (MQ=255)
                                                                                                             gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                                         <  1:811161/149‑1 (MQ=255)
                                                                                                              aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTGATCGAGCTGCTGGCCGCTGATATCCGCCAGCGTCTGACGCCAGGACTCACTGCGCGCCCCGCCCCCAATCCACGGACCACGTTGCGGtgttgt                                        >  2:4721574/1‑144 (MQ=255)
                                                                                                              aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGGAGTCGAGCTGGTGTCCGCTGCTGTCCGCCAGCATCTGGCGCCAGTACTCACTCCGCGCCCCGCCCCCCATCAACGTAACCCGTTGCGGTTTAAt                                        >  1:3264776/1‑149 (MQ=255)
                                                                                                               tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                                       <  1:1860790/149‑1 (MQ=255)
                                                                                                                cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                      <  2:3826376/149‑1 (MQ=255)
                                                                                                                cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                      <  1:2552490/149‑1 (MQ=255)
                                                                                                                cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                      <  2:2193953/149‑1 (MQ=255)
                                                                                                                 tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                     <  2:3734705/149‑1 (MQ=255)
                                                                                                                     ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                                 <  2:2351600/149‑1 (MQ=255)
                                                                                                                       aGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc                               <  1:4245447/149‑1 (MQ=255)
                                                                                                                        gCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCC‑CCCCGTACGGTGATAGAGCTGCTGTCCTGTGATATGCGCGAGCATGTGTCGCGAGTACTGACGACGGGCCCCGCCGCCAATCAAGGTAACTCTTTGCGGGTTTTTACCgggggc                               >  2:2910389/1‑144 (MQ=255)
                                                                                                                        gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa                              <  1:4361483/149‑1 (MQ=255)
                                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                             <  2:2536205/149‑1 (MQ=255)
                                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                             <  1:991864/149‑1 (MQ=255)
                                                                                                                          cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg                            <  1:1896410/149‑1 (MQ=255)
                                                                                                                            tgctgcGCCCAGTGCTGGCCCCACCT‑CCCC‑CACCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                          <  2:4550513/149‑1 (MQ=255)
                                                                                                                            tgctgcGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                          <  1:2064169/149‑1 (MQ=255)
                                                                                                                                 cgcCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                     <  1:4673419/149‑1 (MQ=255)
                                                                                                                                  gcCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTACCACTTTGCGGTTTAATACCGCCGGCATGCACGACAt                    >  1:3676549/1‑149 (MQ=255)
                                                                                                                                    ccAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTAATACCGCAGGCCTGCACGACATcc                  >  1:2154746/1‑149 (MQ=255)
                                                                                                                                          cTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCa            <  1:718100/149‑1 (MQ=255)
                                                                                                                                           tGGCCCCACATCCCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCa            <  1:1086169/149‑1 (MQ=255)
                                                                                                                                               cccACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGc       <  1:3609531/149‑1 (MQ=255)
                                                                                                                                                    aT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg  >  1:1824744/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCG  >  CP009273/3721321‑3721617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑