Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,480 T→G 7.4% T418P (ACG→CCG)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4800TG 7.4% 694.7 / 10.0 288T418P (ACG→CCG) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (85/174);  new base G (27/2);  total (112/176)
Fisher's exact test for biased strand distribution p-value = 1.86e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

GCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACG  >  CP009273/3721333‑3721628
                                                                                                                                                    |                                                                                                                                                    
gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCCT‑CCCCCCCCGtg                                                                                                                                                     >  1:2804103/1‑148 (MQ=255)
   gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACTT‑CCCCCCCCGTACgg                                                                                                                                                  >  1:1057345/1‑149 (MQ=255)
   gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACgg                                                                                                                                                  >  1:3057083/1‑149 (MQ=255)
   gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACgg                                                                                                                                                  >  1:4169211/1‑149 (MQ=255)
     tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTa                                                                                                                                                <  2:810271/149‑1 (MQ=255)
     tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGCCGGTa                                                                                                                                                >  2:3756561/1‑149 (MQ=255)
       aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACgggggt                                                                                                                                              >  2:1548978/1‑145 (MQ=255)
        gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCACTGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTaagc                                                                                                                                             <  2:1899766/149‑3 (MQ=255)
          ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGACGGTCCTCGa                                                                                                                                           >  1:2403736/1‑149 (MQ=255)
           gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGCCGGtgctcggg                                                                                                                                          >  1:1169265/1‑142 (MQ=255)
            cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgc                                                                                                                                         <  2:1837618/149‑1 (MQ=255)
            cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgc                                                                                                                                         <  1:333181/149‑1 (MQ=255)
            cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgc                                                                                                                                         <  1:2502194/149‑1 (MQ=255)
                 cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGctgctg                                                                                                                                    <  1:1797560/149‑1 (MQ=255)
                  gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGTCGGCGAGctgctgg                                                                                                                                   >  2:204708/1‑148 (MQ=255)
                      cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                                               <  1:1465339/149‑1 (MQ=255)
                       gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                              <  2:1035515/149‑1 (MQ=255)
                         aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CGCCCCCCGTCCGGGCCTCGGGCTGGTGGCCGcggt                                                                                                                            >  2:4565773/1‑146 (MQ=255)
                         aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGa                                                                                                                            <  2:4307074/149‑1 (MQ=255)
                         aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGa                                                                                                                            <  2:2170430/149‑1 (MQ=255)
                            tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                                                         <  1:2628458/149‑1 (MQ=255)
                              gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                       <  2:2337872/149‑1 (MQ=255)
                              gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                       <  2:3153006/149‑1 (MQ=255)
                               tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTCTTCGAGCTGCTGACCGCGGAGATCCg                                                                                                                      >  2:4081312/1‑149 (MQ=255)
                                aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                                                     <  1:376985/149‑1 (MQ=255)
                                  aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCGGATATCCGCCa                                                                                                                   >  2:4259058/1‑149 (MQ=255)
                                      gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                               <  1:1552773/149‑1 (MQ=255)
                                      gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                               <  1:2304247/149‑1 (MQ=255)
                                       aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGACGGGAATCGAGCTGCTGCCCGCGGATATCCGCCCGCATc                                                                                                              >  1:3567725/1‑149 (MQ=255)
                                       aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGTCATCGAGCGGCGGACCGCTGATATccgcccgctgc                                                                                                              >  2:2530172/1‑146 (MQ=255)
                                       aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                              <  2:3400385/149‑1 (MQ=255)
                                        cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                             <  1:4373166/149‑1 (MQ=255)
                                        cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGCTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                             <  1:1983296/149‑1 (MQ=255)
                                              tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                                                       <  2:3721420/149‑1 (MQ=255)
                                                      gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGGGCTGCGGTCCGCGGATATCCGCCCGCATGTGACGCCAGTCCTcg                                                                                               >  1:616858/1‑148 (MQ=255)
                                                      gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGTGGGGAATCGGGGTGCTGCCCGCTGCTATCCGCCAGCATCTGACGCCGGGACtga                                                                                               >  2:2305515/1‑147 (MQ=255)
                                                       ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                              <  1:2124733/149‑1 (MQ=255)
                                                       ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                              <  2:3285572/149‑1 (MQ=255)
                                                        tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                             <  1:1194098/149‑1 (MQ=255)
                                                         gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                            <  1:2184330/149‑1 (MQ=255)
                                                          cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                           <  1:231388/149‑1 (MQ=255)
                                                          cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                           <  2:3496659/149‑1 (MQ=255)
                                                           ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCGGATGTCCGCCCGCATCGGGCGCCCGTACTCGCTAcg                                                                                          >  1:1234040/1‑149 (MQ=255)
                                                              aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCGCCCGTGCGGTCATCGCGCTGCTGGCCGCTGATCTCCGCGCGCGTCTGACGCCGGTCCGCAGGAcgcgg                                                                                       >  2:3838571/1‑148 (MQ=255)
                                                              aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCGGCTGACCGCGGAGATCCGCCAGCATCTGACGCCAGGCCTCACTACgcgc                                                                                       >  2:1449384/1‑149 (MQ=255)
                                                                caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                                                     <  1:3978208/149‑1 (MQ=255)
                                                                 aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGACTCGAGCTGGTGACCGCTGGTATCCGCCCGCATCTGACGCCAGTCCTCACTCCGcgcccc                                                                                    >  1:4432848/1‑149 (MQ=255)
                                                                  aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGGAATCGGGCTGCTGAGCGGTGATATCCGCCCGCCTCTGACGCCAGTCCTCACGACGcgccccg                                                                                   >  2:2830462/1‑149 (MQ=255)
                                                                   ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATTGCCGCCAGCCGCTGACGCCAGTTCTCACTCCGcgccccgc                                                                                  >  1:2651163/1‑149 (MQ=255)
                                                                        tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCGGACCGCTGTTATCCGCCAGCCTCTGACGCCCGTACTCACTACGCGCCCCGcccccc                                                                             >  1:2686599/1‑148 (MQ=255)
                                                                         gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                            <  1:877139/149‑1 (MQ=255)
                                                                         gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                            <  2:4658350/149‑1 (MQ=255)
                                                                         gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTTAGCGCGCTGCTGACCGGTGTGATCCGCCCGCCTCTGACGGCTGGGCGGCCTGCGCGCCCCGccccccc                                                                            >  2:1515267/1‑147 (MQ=255)
                                                                              aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCCT‑CCCCCCCCGGCCGGTAATCGAGCTGCGGGCCGCGGGTGTCCGCCCGGGTCTGTGGCCAGTAGTCGCGACGCGCCCCGCCCCCCAGCAAc                                                                       >  2:485142/1‑149 (MQ=255)
                                                                              aTTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                       <  2:615465/149‑1 (MQ=255)
                                                                                ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                                                    <  2:2645914/149‑1 (MQ=255)
                                                                                 tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGCCGGTAATCGAGCTGCTGACCGCTGATCTCCGCCAGCCGCTGACGCCAGTACTCCCTACGCGCCCCGCCCCCACTCCACGtt                                                                    >  1:3203481/1‑148 (MQ=255)
                                                                                      ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACt                                                               <  2:3977414/149‑1 (MQ=255)
                                                                                       gtttCGCCGCGATGTGGGCCTGCCTTGGTGCGCCCTGTTCTGGCCCCACTT‑CCCCCGCCGTAGGGTAATCTAGCTGCTGACCGATGATATCAGCCAGCATCTGACGCCAGTACTCACTTCGCGCCCCGCCCCCAATCACCGTAACACtt                                                              <  2:137308/147‑1 (MQ=255)
                                                                                       gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGACGGGAATCGGGCGGCGGGCCGCGGGTATGCGCCCGCGTGTGGCGCCGGTTCTCGCGACGCGGCCCGGCGCCCAGCCACGTAgcacgt                                                              >  2:3773175/1‑147 (MQ=255)
                                                                                       gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGTGCGGTAATCGCGCTGCTGACCGCGGATATCCGCCCGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAacacgt                                                              >  1:1100025/1‑147 (MQ=255)
                                                                                        aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATGGCGCTGCTGTCCGCTGATATCCGCCAGCTTCTGACGCCAGTGGTCACTACGCGCCCCGCCCCCCATCGACGTGACACttt                                                             >  2:4440695/1‑149 (MQ=255)
                                                                                          tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCACGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                           <  2:4528942/149‑1 (MQ=255)
                                                                                            gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg                                                         <  1:4511281/149‑1 (MQ=255)
                                                                                             ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCGGTCCGCTGATAGCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCggg                                                        >  1:3151781/1‑148 (MQ=255)
                                                                                              cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                       <  1:530333/149‑1 (MQ=255)
                                                                                               gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                      >  2:3009715/1‑149 (MQ=255)
                                                                                                 gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                                                    <  1:811161/149‑1 (MQ=255)
                                                                                                   tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                                                  <  1:1860790/149‑1 (MQ=255)
                                                                                                    cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                 <  1:2552490/149‑1 (MQ=255)
                                                                                                    cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                 <  2:2193953/149‑1 (MQ=255)
                                                                                                     tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                <  2:958606/149‑1 (MQ=255)
                                                                                                          cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAgg                                           <  1:1335956/149‑1 (MQ=255)
                                                                                                           aGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc                                          <  1:4245447/149‑1 (MQ=255)
                                                                                                            gCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCGTACGGTGATAGAGCTGCTGTCCTGTGATATGCGCGAGCATGTGTCGCGAGTACTGACGACGGGCCCCGCCGCCAATCAAGGTAACTCTTTGCGGGTTTTTACCgggggc                                          >  2:2910389/1‑144 (MQ=255)
                                                                                                            gCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCGGCCGGGAGTCGGGCGGGGGGCCGCGGATGTCCGCCCGCCGCGGCCGCCAGTACTCCCTACGCGCCCCGCCCCCCCGCCACGTACGTCTGTGCGGTGTAATACCGCAGGc                                          >  2:237288/1‑149 (MQ=255)
                                                                                                            gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa                                         <  1:919242/149‑1 (MQ=255)
                                                                                                             ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                                        <  1:991864/149‑1 (MQ=255)
                                                                                                              cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg                                       <  1:1896410/149‑1 (MQ=255)
                                                                                                              cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGCATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCACCGTACCCCTTTGCGGGTGAATACCGCAGGCCTg                                       >  2:3236845/1‑149 (MQ=255)
                                                                                                               ttGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc                                      <  2:3298701/149‑1 (MQ=255)
                                                                                                               ttGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc                                      <  2:729533/149‑1 (MQ=255)
                                                                                                                tgctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                                     <  1:2063032/149‑1 (MQ=255)
                                                                                                                   tgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa                                  <  1:3006264/149‑1 (MQ=255)
                                                                                                                   tgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa                                  <  2:4722063/149‑1 (MQ=255)
                                                                                                                     cgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                <  1:4673363/149‑1 (MQ=255)
                                                                                                                      gcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTACCACTTTGCGGTTTAATACCGCCGGCATGCACGACAt                               >  1:3676549/1‑149 (MQ=255)
                                                                                                                      gcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACAt                               <  1:557876/149‑1 (MQ=255)
                                                                                                                          aGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                           <  1:4588126/149‑1 (MQ=255)
                                                                                                                              cTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCa                       <  1:718100/149‑1 (MQ=255)
                                                                                                                               tGGCCCCACATCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCa                       <  1:1086169/149‑1 (MQ=255)
                                                                                                                                 gCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                    <  2:783261/149‑1 (MQ=255)
                                                                                                                                   cccACAT‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTTACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACTCTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGc                  <  1:4730955/149‑1 (MQ=255)
                                                                                                                                   cccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGc                  <  1:2333159/149‑1 (MQ=255)
                                                                                                                                    ccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                 >  1:3882922/1‑149 (MQ=255)
                                                                                                                                     cacaT‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa                <  2:1609393/149‑1 (MQ=255)
                                                                                                                                         t‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcgc            <  2:3799696/149‑1 (MQ=255)
                                                                                                                                             ccccccGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa         <  2:974574/149‑1 (MQ=255)
                                                                                                                                                cccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAcc      >  1:3621737/1‑149 (MQ=255)
                                                                                                                                                    tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACg  >  2:2758737/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACG  >  CP009273/3721333‑3721628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑