Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,485 T→G 8.5% Y416S (TAC→TCC)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4850TG 8.5% 653.7 / 6.2 288Y416S (TAC→TCC) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (80/174);  new base G (31/3);  total (111/177)
Fisher's exact test for biased strand distribution p-value = 1.53e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

ATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTC  >  CP009273/3721337‑3721633
                                                                                                                                                      |                                                                                                                                                    
aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGt                                                                                                                                                      <  1:1068350/149‑1 (MQ=255)
   aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGGGCGGttgg                                                                                                                                                   >  1:2193953/1‑146 (MQ=255)
    gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCACTGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTaagc                                                                                                                                                  <  2:1899766/149‑3 (MQ=255)
       gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGGCCGGtgctcggg                                                                                                                                               >  1:1169265/1‑142 (MQ=255)
        cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAgc                                                                                                                                              <  2:1837618/149‑1 (MQ=255)
         aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCATAT‑CCCC‑CCCCGTACGGTAATCGAgct                                                                                                                                             <  1:4322073/149‑1 (MQ=255)
         aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTGCGGTAATCGAgcg                                                                                                                                             >  2:806370/1‑148 (MQ=255)
             cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGctgctg                                                                                                                                         <  1:1797560/149‑1 (MQ=255)
                  cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                                                    <  1:1465339/149‑1 (MQ=255)
                   gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                                   <  2:1035515/149‑1 (MQ=255)
                     aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CGCC‑CCCCGTCCGGGCCTCGGGCTGGTGGCCGcggt                                                                                                                                 >  2:4565773/1‑146 (MQ=255)
                     aTCTGGTAGAGGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGa                                                                                                                                 <  2:3324115/149‑1 (MQ=255)
                        tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                                                              <  1:2628458/149‑1 (MQ=255)
                          gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                            <  2:2337872/149‑1 (MQ=255)
                          gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                            <  2:3153006/149‑1 (MQ=255)
                              aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                                        <  1:4375553/149‑1 (MQ=255)
                               tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTCCGGGAATCGAGCTGCTGACCGCTGATATCCGGCAg                                                                                                                       >  2:3519642/1‑149 (MQ=255)
                                  gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                                    <  1:1552773/149‑1 (MQ=255)
                                  gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                                    <  1:2304313/149‑1 (MQ=255)
                                   aCTGTTCTACCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCGTGCTGCGCCCAGTGCTGGCCCCCCAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                                   <  2:3400391/149‑1 (MQ=255)
                                   aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTGCGGTCATCGAGCGGCGGACCGCTGATATccgcccgctgc                                                                                                                   >  2:2530172/1‑146 (MQ=255)
                                    cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGCTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                                  <  1:1983296/149‑1 (MQ=255)
                                          tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGGCCGGTGGGCGGGGTGCTGGCCGCGGTTCTCCGCCCGCCTCTGACGcc                                                                                                            >  1:4682828/1‑149 (MQ=255)
                                           aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                                                           <  1:740924/149‑1 (MQ=255)
                                               gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTCCGGTCATCGAGCTGCTGCCCGCTGATATCCGCCAGCATCTGACGCCCGtgc                                                                                                       >  2:1506670/1‑147 (MQ=255)
                                                  gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGGAATCGGGCTGCGGTCCGCGGATATCCGCCCGCATGTGACGCCAGTCCTcg                                                                                                    >  1:616858/1‑148 (MQ=255)
                                                  gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGGTGGGGAATCGGGGTGCTGCCCGCTGCTATCCGCCAGCATCTGACGCCGGGACtga                                                                                                    >  2:2305515/1‑147 (MQ=255)
                                                   ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                                   <  2:3285572/149‑1 (MQ=255)
                                                    tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                                  <  1:1194098/149‑1 (MQ=255)
                                                      cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                                <  1:3709270/149‑1 (MQ=255)
                                                       ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGGCCGCGGATGTCCGCCCGCATCGGGCGCCCGTACTCGCTAcg                                                                                               >  1:1234040/1‑149 (MQ=255)
                                                       ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGTCCGCTGTTGTCCGCCAGCATCTGGCGCCAGTACTCACTAcg                                                                                               >  1:4762463/1‑149 (MQ=255)
                                                          aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACgcgc                                                                                            <  2:21378/149‑1 (MQ=255)
                                                            caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                                                          <  1:2504974/149‑1 (MQ=255)
                                                              aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTGCGGGAATCGGGCTGCTGAGCGGTGATATCCGCCCGCCTCTGACGCCAGTCCTCACGACGcgccccg                                                                                        >  2:2830462/1‑149 (MQ=255)
                                                               ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTACGGTACTCGAGCTGCTGACCGCTGATTGCCGCCAGCCGCTGACGCCAGTTCTCACTCCGcgccccgc                                                                                       >  1:2651163/1‑149 (MQ=255)
                                                               ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTGATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCGAGTACGCACTACGcgccccgc                                                                                       >  2:1541449/1‑149 (MQ=255)
                                                                gccaTGAGCGATTTCTCGGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                                                      <  2:4292700/146‑1 (MQ=255)
                                                                    tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGGAATCGAGCTGCGGACCGCTGTTATCCGCCAGCCTCTGACGCCCGTACTCACTACGCGCCCCGcccccc                                                                                  >  1:2686599/1‑148 (MQ=255)
                                                                     gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTTCTGGACCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGTTGATATCCGCCAGCATCTGACGCCAGTAATCACTACGCGCCCCGCCCCCaa                                                                                 <  2:1929440/149‑1 (MQ=255)
                                                                     gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTGCGGGAATGGGGCGGCTGTCCGGTGTTGGCCGCCCGCATGTGTGGGCAGTTCTGACGACGCGGCCCGgccccca                                                                                 >  2:840152/1‑147 (MQ=255)
                                                                     gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTCAGCGAGCTGCTGGCCGCTGATATCCGCCAGCCTCTGTCGCCAGTTCTCACTACGCGCCCCGccccccc                                                                                 >  1:3973536/1‑147 (MQ=255)
                                                                     gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                                 <  2:4658350/149‑1 (MQ=255)
                                                                      aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGGCCGGGAATCGAGCTGCTGGCCGCGGTTTTCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGcccccgcg                                                                                >  1:3525897/1‑146 (MQ=255)
                                                                          aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCCT‑CCCC‑CCCCGGCCGGTAATCGAGCTGCGGGCCGCGGGTGTCCGCCCGGGTCTGTGGCCAGTAGTCGCGACGCGCCCCGCCCCCCAGCAAc                                                                            >  2:485142/1‑149 (MQ=255)
                                                                          aTTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                            <  2:615465/149‑1 (MQ=255)
                                                                            ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                                          <  1:2403623/149‑1 (MQ=255)
                                                                            ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                                          <  1:3043481/149‑1 (MQ=255)
                                                                            ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                                                         <  2:2645914/149‑1 (MQ=255)
                                                                               tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCGC‑CCCCGTACGGTAATCGGGCTGCTGAGCGCTGTTATCCGCCAGCATCTGACGCCGGTACTCGCTGCGCGCCCCGCCCCCCCTCAACGGAac                                                                       >  1:3652461/1‑149 (MQ=255)
                                                                                   gtttCGCCGCGATGTGGGCCTGCCTTGGTGCGCCCTGTTCTGGCCCCACTT‑CCCC‑CGCCGTAGGGTAATCTAGCTGCTGACCGATGATATCAGCCAGCATCTGACGCCAGTACTCACTTCGCGCCCCGCCCCCAATCACCGTAACACtt                                                                   <  2:137308/147‑1 (MQ=255)
                                                                                   gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGGACGGGAATCGGGCGGCGGGCCGCGGGTATGCGCCCGCGTGTGGCGCCGGTTCTCGCGACGCGGCCCGGCGCCCAGCCACGTAgcacgt                                                                   >  2:3773175/1‑147 (MQ=255)
                                                                                   gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGGCCGGTACGCGCGCGGCTGCCCGCTGATATCCGCCCGCATCTGACGCCAGGACGCCCTACGCGCCCCGCCCGCCAGCAACGGAgcacgg                                                                   >  2:3772492/1‑147 (MQ=255)
                                                                                   gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCC‑CCCCGTGCGGTAATCGCGCTGCTGACCGCGGATATCCGCCCGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAacacgt                                                                   >  1:1100025/1‑147 (MQ=255)
                                                                                    aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGGACGGTAATGGCGCTGCTGTCCGCTGATATCCGCCAGCTTCTGACGCCAGTGGTCACTACGCGCCCCGCCCCCCATCGACGTGACACttt                                                                  >  2:4440695/1‑149 (MQ=255)
                                                                                     ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTGCGGGAATCGAGCTGCTGACCGCGGTTGTCCGCCAGCATCTGACGCCCGGACTCACTACGCGCCCCGCCCCCCATCAACGTAACCCTTTg                                                                 >  1:2328812/1‑149 (MQ=255)
                                                                                     ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                                                 <  2:4032765/149‑1 (MQ=255)
                                                                                      tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCACGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                                <  2:4528942/149‑1 (MQ=255)
                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg                                                              <  1:4511281/149‑1 (MQ=255)
                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCGCCCCGTGCGGGGAGCGAGCTGCGGCCCGCGGTGGTCCGCCCGCATCTGTCGCCGGGACGGACGGCGCGGGCCGCGCGCAAGCCACGTTACACTTGGCg                                                               >  2:754050/1‑149 (MQ=255)
                                                                                         ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCC‑CCCCGGACGGGAATCGAGCTGCGGGCCGCGGAGAGCCGCCAGCATCTGACGCCAGGACTCCCGACGCGCCCCGCCCCCACGCAACGTAACACGTTGCggg                                                             >  1:3151591/1‑148 (MQ=255)
                                                                                          cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                            <  1:530333/149‑1 (MQ=255)
                                                                                           gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                           >  2:3009715/1‑149 (MQ=255)
                                                                                               tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                                                       <  1:1860790/149‑1 (MQ=255)
                                                                                                cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                      <  1:2552490/149‑1 (MQ=255)
                                                                                                cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                      <  2:2193953/149‑1 (MQ=255)
                                                                                                 tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                     <  2:3734705/149‑1 (MQ=255)
                                                                                                      cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAgg                                                <  1:1335956/149‑1 (MQ=255)
                                                                                                        gCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCC‑CCCCGTACGGTGATAGAGCTGCTGTCCTGTGATATGCGCGAGCATGTGTCGCGAGTACTGACGACGGGCCCCGCCGCCAATCAAGGTAACTCTTTGCGGGTTTTTACCgggggc                                               >  2:2910389/1‑144 (MQ=255)
                                                                                                        gCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCC‑CCCCGGCCGGGAGTCGGGCGGGGGGCCGCGGATGTCCGCCCGCCGCGGCCGCCAGTACTCCCTACGCGCCCCGCCCCCCCGCCACGTACGTCTGTGCGGTGTAATACCGCAGGc                                               >  2:237288/1‑149 (MQ=255)
                                                                                                        gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa                                              <  1:919242/149‑1 (MQ=255)
                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                                             <  1:991864/149‑1 (MQ=255)
                                                                                                          cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg                                            <  1:1896410/149‑1 (MQ=255)
                                                                                                          cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGGCATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCACCGTACCCCTTTGCGGGTGAATACCGCAGGCCTg                                            >  2:3236845/1‑149 (MQ=255)
                                                                                                           ttGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc                                           <  2:729533/149‑1 (MQ=255)
                                                                                                            tgctgcGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                                          <  1:845923/149‑1 (MQ=255)
                                                                                                            tgctgcGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                                          <  1:2064169/149‑1 (MQ=255)
                                                                                                               tgcGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGGAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa                                       <  2:4722063/149‑1 (MQ=255)
                                                                                                                 cgcCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                     <  1:4673348/149‑1 (MQ=255)
                                                                                                                 cgcCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                     <  2:3906764/149‑1 (MQ=255)
                                                                                                                  gcCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACAt                                    <  1:557876/149‑1 (MQ=255)
                                                                                                                   cccAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                                   >  1:3331012/1‑149 (MQ=255)
                                                                                                                    ccAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTAATACCGCAGGCCTGCACGACATcc                                  >  1:2154746/1‑149 (MQ=255)
                                                                                                                     cAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGGAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                                 <  2:4137948/149‑1 (MQ=255)
                                                                                                                         gCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGcc                             <  1:4439092/149‑1 (MQ=255)
                                                                                                                               cccACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGc                       <  1:3609531/149‑1 (MQ=255)
                                                                                                                                 cacaT‑CCCC‑CCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa                     <  2:1609393/149‑1 (MQ=255)
                                                                                                                                   caT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgc                   >  1:3880794/1‑149 (MQ=255)
                                                                                                                                    acatccc‑ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgc                   <  1:4517858/145‑1 (MQ=255)
                                                                                                                                     t‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCAGACGCCAGTACACACTACGCGCCCCGCCCCCAATCAAAGTAACACTTTGCGGTTTAAAACCGCAGGCATGCACGACATCCATCCCATCTGCCAgcgc                 >  1:4163450/1‑149 (MQ=255)
                                                                                                                                     t‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcgc                 <  2:3799696/149‑1 (MQ=255)
                                                                                                                                        ccc‑ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAt               <  1:2450775/149‑1 (MQ=255)
                                                                                                                                         cc‑ccccGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa              <  2:974574/149‑1 (MQ=255)
                                                                                                                                               cGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCa         <  1:3188095/149‑1 (MQ=255)
                                                                                                                                                 tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACg       >  2:2758737/1‑149 (MQ=255)
                                                                                                                                                     gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCtt   >  2:3827715/1‑149 (MQ=255)
                                                                                                                                                     gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCtt   >  2:1438810/1‑149 (MQ=255)
                                                                                                                                                     gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCtt   <  1:2841407/149‑1 (MQ=255)
                                                                                                                                                     gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCtt   >  1:1094941/1‑149 (MQ=255)
                                                                                                                                                      tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc  <  1:634219/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
ATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCC‑CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTC  >  CP009273/3721337‑3721633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑