BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,740,731	G→T	5.6%	G96G (GGG→GGT)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,740,731	0	G	T	5.6%	1050.1 / 23.0	268	G96G (GGG→GGT)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base G (137/116); new base T (6/9); total (143/125)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 272 total aligned reads displayed.

TATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCA  >  CP009273/3740584‑3740877

 atGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGaa                                                                                                                                                  <  1:4723904/149‑1 (MQ=255)

  tGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAt                                                                                                                                                 <  1:3986727/149‑1 (MQ=255)

   ggCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATg                                                                                                                                                >  2:609884/1‑149 (MQ=255)

       gAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAt                                                                                                                                            <  2:2001046/149‑1 (MQ=255)

        aaCTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAtt                                                                                                                                           <  1:4761572/149‑1 (MQ=255)

          cTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAtttt                                                                                                                                         <  2:1814843/149‑1 (MQ=255)

              ggCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTcc                                                                                                                                     >  2:3589097/1‑149 (MQ=255)

                cAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTAGCGGCAGCTCCGCACAGGGAAAGGGGTTGTTTTGGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTc                                                                                                                                   <  2:2822106/149‑1 (MQ=255)

                   tgctgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGa                                                                                                                                <  2:1265231/149‑1 (MQ=255)

                   tgctgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGa                                                                                                                                <  1:2321107/149‑1 (MQ=255)

                      tgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAccg                                                                                                                             >  2:765976/1‑149 (MQ=255)

                      tgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAccg                                                                                                                             >  2:1929924/1‑149 (MQ=255)

                       gcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAccgc                                                                                                                            >  2:4099388/1‑149 (MQ=255)

                               gTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGAt                                                                                                                    >  2:3918638/1‑149 (MQ=255)

                                 cATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAATTTGTCGGTATCGGCATCTCCGCACAGGTAAAGGGCTTGTTTTTGCTGGTTAAAAACTAAAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATgg                                                                                                                  <  1:661318/149‑1 (MQ=255)

                                  aTTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGa                                                                                                                 >  1:1568175/1‑149 (MQ=255)

                                     cgcgCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGAGGGAAAt                                                                                                              >  1:1338102/1‑149 (MQ=255)

                                      gcgcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtc                                                                                                             >  2:2307404/1‑149 (MQ=255)

                                        gcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgt                                                                                                           <  1:3500136/149‑1 (MQ=255)

                                        gcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgt                                                                                                           <  2:391679/149‑1 (MQ=255)

                                         cccTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgtt                                                                                                          <  1:2772835/149‑1 (MQ=255)

                                         cccTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgtt                                                                                                          >  2:2998345/1‑149 (MQ=255)

                                           cTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgttcg                                                                                                        >  2:2690812/1‑149 (MQ=255)

                                           cTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgttcg                                                                                                        >  2:3699417/1‑149 (MQ=255)

                                            tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGCAAtcgttcgt                                                                                                       >  2:3639270/1‑149 (MQ=255)

                                            tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgttcgt                                                                                                       <  2:463812/149‑1 (MQ=255)

                                             gCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTtcgtc                                                                                                      <  2:1499113/149‑1 (MQ=255)

                                               ttACTCATTCTGGTGTTAGCGGGGAACAATTTGTCGGTATCGGCATCTCCGCACTGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGc                                                                                                    <  1:4128398/149‑1 (MQ=255)

                                               ttACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGc                                                                                                    <  1:1354693/149‑1 (MQ=255)

                                                  cTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTgg                                                                                                 >  1:1965965/1‑149 (MQ=255)

                                                  cTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTgg                                                                                                 >  1:2553821/1‑149 (MQ=255)

                                                    cATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCa                                                                                               >  1:3547358/1‑149 (MQ=255)

                                                          ggTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGa                                                                                         <  2:218029/149‑1 (MQ=255)

                                                          ggTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGa                                                                                         <  1:2235349/149‑1 (MQ=255)

                                                           gtgtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGAt                                                                                        >  1:1918966/1‑149 (MQ=255)

                                                            tgtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATg                                                                                       <  1:3101108/149‑1 (MQ=255)

                                                                aGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCAt                                                                                   >  1:81914/1‑149 (MQ=255)

                                                                  cGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATcc                                                                                 <  1:752411/149‑1 (MQ=255)

                                                                   ggggAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATccc                                                                                >  1:3026473/1‑149 (MQ=255)

                                                                   ggggAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATccc                                                                                >  1:4184809/1‑149 (MQ=255)

                                                                   ggggAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATccc                                                                                >  1:3241106/1‑149 (MQ=255)

                                                                    gggAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCg                                                                               >  1:3701132/1‑149 (MQ=255)

                                                                    gggAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCg                                                                               >  1:3818845/1‑149 (MQ=255)

                                                                       aaCAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaa                                                                            >  1:4277506/1‑149 (MQ=255)

                                                                        aCAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaaa                                                                           >  1:4615096/1‑149 (MQ=255)

                                                                         cAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaaaa                                                                          >  1:900173/1‑149 (MQ=255)

                                                                          aaaTTGTCGGTATCGGCTTCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACACACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaaaaa                                                                         <  1:2615141/149‑1 (MQ=255)

                                                                           aaTTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAAc                                                                        <  1:4374398/149‑1 (MQ=255)

                                                                           aaTTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAAc                                                                        <  2:1702636/149‑1 (MQ=255)

                                                                             ttGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAActc                                                                      >  1:4037414/1‑149 (MQ=255)

                                                                             ttGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAActc                                                                      >  1:1649668/1‑149 (MQ=255)

                                                                                tCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTAc                                                                   <  1:1176384/149‑1 (MQ=255)

                                                                                 cGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTAcc                                                                  <  2:1518865/149‑1 (MQ=255)

                                                                                  ggTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTAccc                                                                 <  2:1228905/149‑1 (MQ=255)

                                                                                  ggTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTAccc                                                                 >  2:1014810/1‑149 (MQ=255)

                                                                                    tATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGc                                                               <  1:2921352/149‑1 (MQ=255)

                                                                                     aTCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCt                                                              >  2:901056/1‑149 (MQ=255)

                                                                                      tCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTg                                                             >  1:4482416/1‑149 (MQ=255)

                                                                                         gCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGAcc                                                          >  2:4508031/1‑149 (MQ=255)

                                                                                            tctcCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGa                                                       >  1:4446570/1‑149 (MQ=255)

                                                                                             ctcCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGAc                                                      <  2:3797761/149‑1 (MQ=255)

                                                                                              tcCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACa                                                     >  1:2081875/1‑149 (MQ=255)

                                                                                               ccGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACaa                                                    >  2:2043532/1‑149 (MQ=255)

                                                                                                cGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACaaa                                                   <  1:1725060/149‑1 (MQ=255)

                                                                                                 gCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAAc                                                  <  2:3501192/149‑1 (MQ=255)

                                                                                                 gCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAAc                                                  >  1:3453223/1‑149 (MQ=255)

                                                                                                  cacaGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAAcc                                                 >  2:662141/1‑149 (MQ=255)

                                                                                                   acaGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCt                                                <  2:2377811/149‑1 (MQ=255)

                                                                                                     aGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTtg                                              <  1:3850719/149‑1 (MQ=255)

                                                                                                      gggAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTtgt                                             >  1:652534/1‑149 (MQ=255)

                                                                                                       ggAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTtgtg                                            <  2:4179577/149‑1 (MQ=255)

                                                                                                        gAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTgg                                           >  1:502726/1‑149 (MQ=255)

                                                                                                         aaaGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGa                                          >  1:1034125/1‑149 (MQ=255)

                                                                                                               cTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGTCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGc                                    >  1:3218401/1‑149 (MQ=255)

                                                                                                                     tttGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCgg                              <  2:4645591/149‑1 (MQ=255)

                                                                                                                        gCTGTATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGgtgt                           <  1:1017777/149‑1 (MQ=255)

                                                                                                                        gCTGGATAAAAACGACACACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGgtgt                           <  1:3405219/149‑1 (MQ=255)

                                                                                                                           ggATAAAAACGACAAACCGCTTGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGc                        >  2:466498/1‑149 (MQ=255)

                                                                                                                           ggATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGc                        >  1:1429767/1‑149 (MQ=255)

                                                                                                                           ggATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGc                        <  2:3323264/149‑1 (MQ=255)

                                                                                                                             aTAAAAACGACACACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTg                      <  1:3058507/149‑1 (MQ=255)

                                                                                                                                aaaaCGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTa                   <  2:303833/149‑1 (MQ=255)

                                                                                                                                aaaaCGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTa                   >  2:2591876/1‑149 (MQ=255)

                                                                                                                                 aaaCGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTAc                  >  2:3447792/1‑149 (MQ=255)

                                                                                                                                 aaaCGACAAACCGCTAGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTAc                  >  1:2357526/1‑149 (MQ=255)

                                                                                                                                  aaCGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACg                 >  2:2745298/1‑149 (MQ=255)

                                                                                                                                   aCGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgc                <  1:215932/149‑1 (MQ=255)

                                                                                                                                       cAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctggc            >  1:4675289/1‑149 (MQ=255)

                                                                                                                                        aaaCCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTTGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctggct           >  2:1409408/1‑149 (MQ=255)

                                                                                                                                        aaaCCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctggct           <  2:3968303/149‑1 (MQ=255)

                                                                                                                                         aaCCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACgctggctg          <  2:4254346/149‑1 (MQ=255)

                                                                                                                                          aCCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGa         >  1:1801594/1‑149 (MQ=255)

                                                                                                                                            cGCTCGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATAGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGaaa       >  1:4426424/1‑149 (MQ=255)

                                                                                                                                            cGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGaaa       <  2:3067018/149‑1 (MQ=255)

                                                                                                                                             gCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAag      >  2:469938/1‑149 (MQ=255)

                                                                                                                                               tCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAagag    <  1:398176/149‑1 (MQ=255)

                                                                                                                                               tCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAagag    <  1:3915077/149‑1 (MQ=255)

                                                                                                                                                cGGTAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGc   <  2:4228632/149‑1 (MQ=255)

                                                                                                                                                cGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGc   >  1:2886300/1‑149 (MQ=255)

                                                                                                                                                 gggAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCa  >  2:11591/1‑149 (MQ=255)

TATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCA  >  CP009273/3740584‑3740877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`