BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,791,032	C→T	7.6%	S242F (TCC→TTC)	waaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,791,032	0	C	T	7.6%	963.7 / 44.4	278	S242F (TCC→TTC)	waaL	O‑antigen ligase
Reads supporting (aligned to +/- strand): ref base C (116/138); new base T (9/12); total (126/152)
Fisher's exact test for biased strand distribution p-value = 8.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 285 total aligned reads displayed.

AATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATG  >  CP009273/3790884‑3791180

aaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTc                                                                                                                                                      >  1:3938969/1‑149 (MQ=255)

 atcatcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTcc                                                                                                                                                     <  1:4554932/149‑1 (MQ=255)

     tcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTa                                                                                                                                                 <  2:230413/149‑1 (MQ=255)

     tcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTa                                                                                                                                                 <  2:2008377/149‑1 (MQ=255)

     tcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTa                                                                                                                                                 <  2:1884987/149‑1 (MQ=255)

        ttttttATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTTCTCTATTg                                                                                                                                              >  1:3965972/1‑149 (MQ=255)

        ttttttATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTg                                                                                                                                              >  2:3658999/1‑149 (MQ=255)

          ttttatttttatTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTTCTCTATTGtt                                                                                                                                            <  1:3541552/149‑1 (MQ=255)

             tatttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTcta                                                                                                                                         >  1:4393291/1‑149 (MQ=255)

             tatttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTcta                                                                                                                                         <  2:2320499/149‑1 (MQ=255)

              atttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTctac                                                                                                                                        <  1:4418003/149‑1 (MQ=255)

              atttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTctac                                                                                                                                        <  2:1270815/149‑1 (MQ=255)

              atttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTctac                                                                                                                                        >  1:1358748/1‑149 (MQ=255)

                ttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTctacta                                                                                                                                      <  2:2563128/149‑1 (MQ=255)

                   tattaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAAtt                                                                                                                                   >  2:1721559/1‑149 (MQ=255)

                     ttaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGc                                                                                                                                 <  2:3936178/149‑1 (MQ=255)

                        aaTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCtat                                                                                                                              >  1:2776094/1‑149 (MQ=255)

                        aaTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCtat                                                                                                                              <  2:1004473/149‑1 (MQ=255)

                         aTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCtata                                                                                                                             >  2:1409257/1‑149 (MQ=255)

                          tAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTatat                                                                                                                            >  2:3212491/1‑149 (MQ=255)

                          tAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTatat                                                                                                                            <  2:3463826/149‑1 (MQ=255)

                          tAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTatat                                                                                                                            >  1:796143/1‑149 (MQ=255)

                           aGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATAtt                                                                                                                           >  2:3033611/1‑149 (MQ=255)

                           aGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATAtt                                                                                                                           <  2:4303902/149‑1 (MQ=255)

                            gTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATta                                                                                                                          <  1:101233/149‑1 (MQ=255)

                              tGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATtagc                                                                                                                        <  1:365222/149‑1 (MQ=255)

                                 gcgGTACTTTATGTTCTGGCTCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTACTCTATTGTTCTACTAATTGCTATATtagctag                                                                                                                     <  1:3359200/149‑1 (MQ=255)

                                     tACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCAtt                                                                                                                 >  1:1205655/1‑149 (MQ=255)

                                       cTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGt                                                                                                               <  1:2063803/149‑1 (MQ=255)

                                        tttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGtt                                                                                                              >  1:2807912/1‑149 (MQ=255)

                                        tttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGtt                                                                                                              <  2:2232349/149‑1 (MQ=255)

                                        tttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGtt                                                                                                              <  2:476592/149‑1 (MQ=255)

                                         ttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGtta                                                                                                             <  2:500481/149‑1 (MQ=255)

                                          tATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGttat                                                                                                            <  1:4156942/149‑1 (MQ=255)

                                           aTGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGttatt                                                                                                           <  1:4765940/149‑1 (MQ=255)

                                            tGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGttatta                                                                                                          <  2:1139342/149‑1 (MQ=255)

                                            tGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGttatta                                                                                                          <  2:2859361/149‑1 (MQ=255)

                                                cTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTAGTATCATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATAtt                                                                                                      >  1:1544307/1‑149 (MQ=255)

                                                 tGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATAttt                                                                                                     >  2:2360400/1‑149 (MQ=255)

                                                   gcgcTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTtaa                                                                                                   <  1:2357654/149‑1 (MQ=255)

                                                   gcgcTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTtaa                                                                                                   >  1:626754/1‑149 (MQ=255)

                                                      cTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTTCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTtaataa                                                                                                <  1:688109/149‑1 (MQ=255)

                                                        aaCACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAAc                                                                                              >  1:855501/1‑149 (MQ=255)

                                                          cacaAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCa                                                                                            <  1:1841247/149‑1 (MQ=255)

                                                             aaaCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTTCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATa                                                                                         <  2:933069/149‑1 (MQ=255)

                                                             aaaCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATa                                                                                         >  1:2272598/1‑149 (MQ=255)

                                                              aaCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATAc                                                                                        <  1:1122150/149‑1 (MQ=255)

                                                              aaCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATAc                                                                                        >  1:4036967/1‑149 (MQ=255)

                                                              aaCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGGTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATAc                                                                                        <  1:2801830/149‑1 (MQ=255)

                                                               aCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACa                                                                                       <  2:2766141/149‑1 (MQ=255)

                                                               aCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACa                                                                                       >  2:1939614/1‑149 (MQ=255)

                                                               aCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACa                                                                                       <  2:374365/149‑1 (MQ=255)

                                                                ccAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTTCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAg                                                                                      >  2:2067249/1‑149 (MQ=255)

                                                                   gagCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAAGAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAAt                                                                                   >  2:3580927/1‑149 (MQ=255)

                                                                    agCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATc                                                                                  >  2:4127147/1‑149 (MQ=255)

                                                                        aCCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCta                                                                              <  1:1972383/149‑1 (MQ=255)

                                                                              cTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGa                                                                        <  1:3486612/149‑1 (MQ=255)

                                                                                  tGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCa                                                                    >  1:1746078/1‑149 (MQ=255)

                                                                                  tGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCa                                                                    <  1:3974490/149‑1 (MQ=255)

                                                                                  tGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCa                                                                    <  2:888989/149‑1 (MQ=255)

                                                                                   gTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCAt                                                                   <  2:1318833/149‑1 (MQ=255)

                                                                                    ttCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCAtt                                                                  >  2:902507/1‑149 (MQ=255)

                                                                                     tCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTTCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTa                                                                 >  2:3796775/1‑149 (MQ=255)

                                                                                       ccTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTaaa                                                               <  2:422640/149‑1 (MQ=255)

                                                                                       ccTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTaaa                                                               <  1:2047412/149‑1 (MQ=255)

                                                                                       ccTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTaaa                                                               <  1:464596/149‑1 (MQ=255)

                                                                                        cTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAAt                                                              >  1:3204387/1‑149 (MQ=255)

                                                                                            aaTTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACt                                                          >  2:4481112/1‑149 (MQ=255)

                                                                                            aaTTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACt                                                          >  1:3039068/1‑149 (MQ=255)

                                                                                             aTTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACtt                                                         <  2:3326037/149‑1 (MQ=255)

                                                                                               ttGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTaa                                                       <  1:2395150/149‑1 (MQ=255)

                                                                                                  tgtTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACa                                                    <  1:2532389/149‑1 (MQ=255)

                                                                                                   gtTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAg                                                   <  2:4639873/149‑1 (MQ=255)

                                                                                                              aaTAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATg                                        <  1:1710069/149‑1 (MQ=255)

                                                                                                              aaTAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATg                                        >  2:829376/1‑149 (MQ=255)

                                                                                                                tAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCt                                      <  2:1475747/149‑1 (MQ=255)

                                                                                                                tAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCt                                      <  1:357161/149‑1 (MQ=255)

                                                                                                                tAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCt                                      <  2:3865188/149‑1 (MQ=255)

                                                                                                                 aGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCNa                                     <  1:1639861/149‑1 (MQ=255)

                                                                                                                   cTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAAt                                   <  1:1401179/149‑1 (MQ=255)

                                                                                                                         ataataAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCTATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGtt                             <  2:1585355/149‑1 (MQ=255)

                                                                                                                         ataataAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGtt                             <  2:791531/149‑1 (MQ=255)

                                                                                                                         ataataAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGtt                             <  2:2634075/149‑1 (MQ=255)

                                                                                                                          taataaATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTa                            <  2:569787/149‑1 (MQ=255)

                                                                                                                           aataaATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTAc                           >  1:2654259/1‑149 (MQ=255)

                                                                                                                            ataaATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACt                          >  1:1070184/1‑149 (MQ=255)

                                                                                                                                aTCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTccc                      <  2:4541198/149‑1 (MQ=255)

                                                                                                                                     ccAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGt                 >  1:573557/1‑149 (MQ=255)

                                                                                                                                     ccAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGt                 >  1:472986/1‑149 (MQ=255)

                                                                                                                                      cAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTg                >  1:2916324/1‑149 (MQ=255)

                                                                                                                                        aGAAATTCACTTTCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCa              >  2:214314/1‑149 (MQ=255)

                                                                                                                                         gAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCaa             >  1:2724123/1‑149 (MQ=255)

                                                                                                                                           aaTTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAAAAGTTATACCAATGCTAATAGTGTTACTTCACTAGTTGCAAGa           >  1:4293924/1‑149 (MQ=255)

                                                                                                                                            aTTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGAc          >  1:3783432/1‑149 (MQ=255)

                                                                                                                                             ttCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACt         >  1:426166/1‑149 (MQ=255)

                                                                                                                                              tCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTg        <  2:2272598/149‑1 (MQ=255)

                                                                                                                                              tCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTg        >  2:686951/1‑149 (MQ=255)

                                                                                                                                                 cTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCa     >  2:2701372/1‑149 (MQ=255)

                                                                                                                                                  ttCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCaa    <  2:4564559/149‑1 (MQ=255)

                                                                                                                                                    ccTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATg  >  1:3814258/1‑149 (MQ=255)

AATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATG  >  CP009273/3790884‑3791180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`