BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,834,958	G→T	6.1%	Q48K (CAG→AAG)	nepI ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,834,958	0	G	T	6.1%	1012.6 / 24.7	269	Q48K (CAG→AAG)	nepI	putative transporter
Reads supporting (aligned to +/- strand): ref base G (148/104); new base T (9/8); total (157/112)
Fisher's exact test for biased strand distribution p-value = 8.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

AGCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTA  >  CP009273/3834810‑3835106

agCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTg                                                                                                                                                      <  1:4722257/149‑1 (MQ=255)

 gCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTgg                                                                                                                                                     <  1:2225021/149‑1 (MQ=255)

 gCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTgg                                                                                                                                                     >  2:4580027/1‑149 (MQ=255)

   gTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGc                                                                                                                                                   <  1:4602120/149‑1 (MQ=255)

   gTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGc                                                                                                                                                   <  1:2206109/149‑1 (MQ=255)

      aGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCAt                                                                                                                                                <  2:1400634/149‑1 (MQ=255)

       gCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCAtt                                                                                                                                               <  1:1033956/149‑1 (MQ=255)

       gCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCAtt                                                                                                                                               <  2:3193227/149‑1 (MQ=255)

       gCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCAtt                                                                                                                                               >  1:3427929/1‑149 (MQ=255)

          aaaCGGCAAACAAAATAACACCGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGc                                                                                                                                            <  1:537210/149‑1 (MQ=255)

          aaaCGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGc                                                                                                                                            >  2:4232119/1‑149 (MQ=255)

          aaaCGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGc                                                                                                                                            >  1:2741264/1‑149 (MQ=255)

           aaCGTCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCTACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCg                                                                                                                                           <  1:1615089/149‑1 (MQ=255)

               gCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTcaa                                                                                                                                       >  1:2392171/1‑149 (MQ=255)

                 aaacaaaATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTcaaca                                                                                                                                     <  2:310974/149‑1 (MQ=255)

                     aaaaTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACt                                                                                                                                 <  1:1910685/149‑1 (MQ=255)

                      aaaTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTg                                                                                                                                >  2:3236161/1‑149 (MQ=255)

                      aaaTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTg                                                                                                                                >  1:3237413/1‑149 (MQ=255)

                       aaTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGa                                                                                                                               <  2:2220954/149‑1 (MQ=255)

                       aaTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGa                                                                                                                               >  1:704675/1‑149 (MQ=255)

                       aaTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGa                                                                                                                               >  1:2938096/1‑149 (MQ=255)

                        aTAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGAc                                                                                                                              >  2:3634441/1‑149 (MQ=255)

                          aacaacGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACgg                                                                                                                            <  1:2567440/149‑1 (MQ=255)

                           acaacGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACggg                                                                                                                           >  1:2622461/1‑149 (MQ=255)

                           acaacGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACggg                                                                                                                           >  2:3566240/1‑149 (MQ=255)

                              acGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCaa                                                                                                                        >  2:3809150/1‑149 (MQ=255)

                              acGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCaa                                                                                                                        >  1:1025100/1‑149 (MQ=255)

                              acGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCaa                                                                                                                        >  2:3413584/1‑149 (MQ=255)

                                 tAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCaaaaa                                                                                                                     >  1:1200640/1‑149 (MQ=255)

                                  aGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAAc                                                                                                                    >  1:1274829/1‑149 (MQ=255)

                                     ggcggTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCa                                                                                                                 >  2:3337053/1‑149 (MQ=255)

                                         gTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGa                                                                                                             <  1:1992756/149‑1 (MQ=255)

                                             gTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAAt                                                                                                         >  2:44199/1‑149 (MQ=255)

                                             gTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAAt                                                                                                         >  2:2503874/1‑149 (MQ=255)

                                               aGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCa                                                                                                       <  2:2096770/149‑1 (MQ=255)

                                               aGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCa                                                                                                       <  1:1295056/149‑1 (MQ=255)

                                                gCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAg                                                                                                      >  1:4290192/1‑149 (MQ=255)

                                                   tGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACa                                                                                                   <  1:3344611/149‑1 (MQ=255)

                                                   tGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACa                                                                                                   >  1:1945970/1‑149 (MQ=255)

                                                   tGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACa                                                                                                   >  1:4689704/1‑149 (MQ=255)

                                                      aTTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGc                                                                                                >  2:3851291/1‑149 (MQ=255)

                                                      aTTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGc                                                                                                >  1:2150725/1‑149 (MQ=255)

                                                      aTTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGc                                                                                                >  2:4012610/1‑149 (MQ=255)

                                                       ttGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCg                                                                                               >  2:3044098/1‑149 (MQ=255)

                                                          tCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGaca                                                                                            <  1:1581016/149‑1 (MQ=255)

                                                               gTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACaaaa                                                                                       <  1:3573753/149‑1 (MQ=255)

                                                               gTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACaaaa                                                                                       >  2:2245456/1‑149 (MQ=255)

                                                               gTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGTCAGGCGACACaaaa                                                                                       >  2:1289440/1‑149 (MQ=255)

                                                               gTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACaaaa                                                                                       >  1:1075296/1‑149 (MQ=255)

                                                                taataaACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAAc                                                                                      <  1:4190859/149‑1 (MQ=255)

                                                                   taaACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGcc                                                                                   >  2:2647435/1‑149 (MQ=255)

                                                                    aaacaaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCa                                                                                  <  2:983152/149‑1 (MQ=255)

                                                                    aaacaaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCa                                                                                  <  2:4150133/149‑1 (MQ=255)

                                                                    aaacaaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCa                                                                                  <  2:2344544/149‑1 (MQ=255)

                                                                        aaacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAAACAGACAGGCGACACAAAACGCCACCga                                                                              >  2:2021628/1‑149 (MQ=255)

                                                                         aacTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCgag                                                                             >  1:958932/1‑149 (MQ=255)

                                                                                 aaCATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCATTTGGCGTCAACAACCTGAGGGGAAAAAAATCAACTATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGc                                                                     <  1:843175/149‑1 (MQ=255)

                                                                                   cATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAACACGGCTg                                                                   >  1:4507777/1‑149 (MQ=255)

                                                                                    aTTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTTGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGa                                                                  >  2:3520783/1‑149 (MQ=255)

                                                                                     ttGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGAc                                                                 >  1:4330072/1‑149 (MQ=255)

                                                                                      tGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGAcc                                                                <  2:1245548/149‑1 (MQ=255)

                                                                                          acaAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGtt                                                            <  1:1507122/149‑1 (MQ=255)

                                                                                          acaAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGACATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGACAACGGCTGACCAGtt                                                            >  2:1012580/1‑149 (MQ=255)

                                                                                           caAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGAAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtc                                                           <  1:615172/149‑1 (MQ=255)

                                                                                             aaGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcgg                                                         >  2:3157202/1‑149 (MQ=255)

                                                                                             aaGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTCCCGAAATGCCTAAATCCTGGGCCAGTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACTCCACCGAGAAAACGGCTGACCAGTtcgg                                                         >  1:2651352/1‑149 (MQ=255)

                                                                                              aGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcggt                                                        <  1:988725/149‑1 (MQ=255)

                                                                                               ggCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcggtc                                                       >  2:2432987/1‑149 (MQ=255)

                                                                                               ggCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcggtc                                                       >  1:4080691/1‑149 (MQ=255)

                                                                                               ggCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcggtc                                                       >  2:4329876/1‑149 (MQ=255)

                                                                                                 cggtcacggtcaCCGATGGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTtcggtcgg                                                     <  2:1635540/149‑1 (MQ=255)

                                                                                                    tcacggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTg                                                  >  2:1911874/1‑149 (MQ=255)

                                                                                                     cacggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGa                                                 <  1:2618919/149‑1 (MQ=255)

                                                                                                     cacggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAAACCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGa                                                 <  2:1065540/149‑1 (MQ=255)

                                                                                                      acggtcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGAt                                                <  2:3455259/149‑1 (MQ=255)

                                                                                                          tcacCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCa                                            >  1:2165083/1‑149 (MQ=255)

                                                                                                            acCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATc                                          <  1:504861/149‑1 (MQ=255)

                                                                                                               gATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgc                                       >  1:3680643/1‑149 (MQ=255)

                                                                                                                aTTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgcg                                      >  2:3036616/1‑149 (MQ=255)

                                                                                                                   gCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgcgccg                                   >  2:3009092/1‑149 (MQ=255)

                                                                                                                   gCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgcgccg                                   >  2:2890751/1‑149 (MQ=255)

                                                                                                                    cccGGCACCCCCTGCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTCACGGGCAAAAACTCCACGATAATCAGACAGGCGACACAACACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATccgcgccgc                                  <  2:2019714/149‑1 (MQ=255)

                                                                                                                      cGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCgg                                <  2:689385/149‑1 (MQ=255)

                                                                                                                       ggCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGt                               <  1:313070/149‑1 (MQ=255)

                                                                                                                        gCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGtt                              >  2:2120407/1‑149 (MQ=255)

                                                                                                                        gCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGtt                              >  2:1928939/1‑149 (MQ=255)

                                                                                                                            ccccTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCg                          >  2:2703366/1‑149 (MQ=255)

                                                                                                                               cTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCa                       >  2:4749915/1‑149 (MQ=255)

                                                                                                                                 tCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAACCTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAAt                     <  2:1157896/149‑1 (MQ=255)

                                                                                                                                 tCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAAt                     >  1:3865146/1‑149 (MQ=255)

                                                                                                                                     aaaTGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAAt                 <  1:4392571/149‑1 (MQ=255)

                                                                                                                                      aaTGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAAtt                >  1:3031966/1‑149 (MQ=255)

                                                                                                                                           cTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTc           >  1:2857204/1‑149 (MQ=255)

                                                                                                                                             aaaTCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCAt         >  2:4662957/1‑149 (MQ=255)

                                                                                                                                               aTCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATgg       >  1:2842869/1‑149 (MQ=255)

                                                                                                                                                tCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGgt      <  2:4710771/149‑1 (MQ=255)

                                                                                                                                                tCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGgt      >  2:2048298/1‑149 (MQ=255)

                                                                                                                                                 ccTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGgtg     >  1:4400789/1‑149 (MQ=255)

                                                                                                                                                 ccTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGgtg     <  1:588497/149‑1 (MQ=255)

                                                                                                                                                    gggCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTa  <  2:4114131/149‑1 (MQ=255)

AGCGTCAGCAAAACGGCAAACAAAATAACAACGTAGCGGCGGTCAGTAGCCTGAATTGTCTGGGTAATAAACAAACTGGCAAACATTGCCACAAAGGCGGTCACGGTCACCGATTGCCCGGCAACCCCTTCCGAAATGCCTAAATCCTGGGCCATTGGCGTCAACAAACTGACGGGCAAAAACTCAACGATAATCAGACAGGCGACACAAAACGCCACCGAGAAAACGGCTGACCAGTTCGGTCGGGTGATGGCATCCGCGCCGCGGTTTTCGGCAATAAATTCACTCATGGTGTTA  >  CP009273/3834810‑3835106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`