Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 319,024 C→A 85.3% R326S (CGC→AGC)  ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273319,0240CA85.3% 692.1 / 120.2 300R326S (CGC→AGC) ykgFferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (26/19);  new base A (101/153);  total (127/173)
Fisher's exact test for biased strand distribution p-value = 3.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 301 total aligned reads displayed.

CGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGT  >  CP009273/318878‑319169
                                                                                                                                                  |                                                                                                                                                 
  cgcgAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCa                                                                                                                                               <  1:3228702/149‑1 (MQ=255)
  cgcgAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCa                                                                                                                                               <  1:4001751/149‑1 (MQ=255)
  cgcgAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCa                                                                                                                                               <  1:947779/149‑1 (MQ=255)
   gcgAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCat                                                                                                                                              >  1:2689938/1‑149 (MQ=255)
     gAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCatat                                                                                                                                            <  1:1258241/149‑1 (MQ=255)
      aaGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATAtt                                                                                                                                           <  1:4636137/149‑1 (MQ=255)
      aaGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATAtt                                                                                                                                           <  1:2495067/149‑1 (MQ=255)
       aGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTg                                                                                                                                          >  2:4266512/1‑149 (MQ=255)
        gCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCAGGCATATCGCCATATTgg                                                                                                                                         >  2:4060786/1‑149 (MQ=255)
           gggCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCgg                                                                                                                                      >  1:1169138/1‑149 (MQ=255)
            ggCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGt                                                                                                                                     <  2:2226994/149‑1 (MQ=255)
              cACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCa                                                                                                                                   <  2:1651117/149‑1 (MQ=255)
                 gTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATgg                                                                                                                                <  2:1003993/149‑1 (MQ=255)
                      tGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATg                                                                                                                           >  2:2034650/1‑149 (MQ=255)
                      tGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATg                                                                                                                           >  2:3468261/1‑149 (MQ=255)
                       ggTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATgg                                                                                                                          >  2:1077537/1‑149 (MQ=255)
                        gTCCAGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGc                                                                                                                         <  2:373162/149‑1 (MQ=255)
                                   tttCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATcc                                                                                                              <  1:1398184/149‑1 (MQ=255)
                                   tttCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATcc                                                                                                              >  1:4189867/1‑149 (MQ=255)
                                      cATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAgg                                                                                                           <  1:4662611/149‑1 (MQ=255)
                                        tCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGc                                                                                                         >  1:2704773/1‑149 (MQ=255)
                                        tCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGc                                                                                                         <  2:2240864/149‑1 (MQ=255)
                                         cTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCAGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGcc                                                                                                        <  2:3336917/149‑1 (MQ=255)
                                           ggTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCac                                                                                                      >  2:2818312/1‑148 (MQ=255)
                                           ggTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCaa                                                                                                      >  1:4574545/1‑149 (MQ=255)
                                           ggTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCaa                                                                                                      >  2:2577034/1‑149 (MQ=255)
                                                    cGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCgg                                                                                             <  2:3338581/149‑1 (MQ=255)
                                                     gATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGt                                                                                            <  1:1264196/149‑1 (MQ=255)
                                                      aTAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTg                                                                                           >  1:3126562/1‑149 (MQ=255)
                                                         aCGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGAtt                                                                                        >  2:3833433/1‑149 (MQ=255)
                                                             gCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTtctc                                                                                    <  2:1836046/149‑1 (MQ=255)
                                                             gCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGACATATTGGCGGTCATGGATATGGCTCTATTTATCCATGGCCAATTGGTGCGGTTATTtctc                                                                                    <  1:2892124/149‑1 (MQ=255)
                                                                ttCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGc                                                                                 >  2:233596/1‑149 (MQ=255)
                                                                 tCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCt                                                                                >  2:4003538/1‑149 (MQ=255)
                                                                 tCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCt                                                                                >  2:588614/1‑149 (MQ=255)
                                                                  cTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTCTGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTa                                                                               >  1:2759325/1‑149 (MQ=255)
                                                                   tGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTAc                                                                              >  1:238317/1‑149 (MQ=255)
                                                                     aGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACtt                                                                            <  1:2634232/149‑1 (MQ=255)
                                                                      ggTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTg                                                                           <  2:3126562/149‑1 (MQ=255)
                                                                        tGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                         <  2:2467613/149‑1 (MQ=255)
                                                                        tGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                         <  2:74308/149‑1 (MQ=255)
                                                                        tGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                         <  2:1158404/149‑1 (MQ=255)
                                                                          cTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATAGTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTggcgg                                                                       >  2:2694895/1‑149 (MQ=255)
                                                                            ggCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATAATTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATATCTCCGCTACTTGGCGTCt                                                                     >  2:4683071/1‑149 (MQ=255)
                                                                              ccTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtat                                                                   <  1:4393717/149‑1 (MQ=255)
                                                                               ctctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtata                                                                  >  1:4262292/1‑149 (MQ=255)
                                                                               ctctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtata                                                                  >  2:2936376/1‑149 (MQ=255)
                                                                                 ctGTATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGATTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaa                                                                <  2:3167618/149‑1 (MQ=255)
                                                                                 ctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaa                                                                <  2:775784/149‑1 (MQ=255)
                                                                                 ctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaa                                                                >  1:531446/1‑149 (MQ=255)
                                                                                 ctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACGTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaa                                                                <  1:4766231/149‑1 (MQ=255)
                                                                                   gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaaga                                                              >  1:3738797/1‑149 (MQ=255)
                                                                                     aTTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatt                                                            <  2:326977/149‑1 (MQ=255)
                                                                                      tttCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattt                                                           >  2:2014432/1‑149 (MQ=255)
                                                                                      tttCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattt                                                           <  2:2092488/149‑1 (MQ=255)
                                                                                         cGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaa                                                        <  2:2388991/149‑1 (MQ=255)
                                                                                          gggATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaa                                                       >  2:684560/1‑149 (MQ=255)
                                                                                           ggATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaag                                                      <  2:3738797/149‑1 (MQ=255)
                                                                                            gATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaaga                                                     <  2:4671446/149‑1 (MQ=255)
                                                                                             aTGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagat                                                    >  2:1916816/1‑149 (MQ=255)
                                                                                             aTGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagat                                                    <  1:1368032/149‑1 (MQ=255)
                                                                                               gtgCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagattt                                                  >  2:3936218/1‑149 (MQ=255)
                                                                                                tgctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTa                                                 <  1:3837670/149‑1 (MQ=255)
                                                                                                 gctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAc                                                <  1:3024451/149‑1 (MQ=255)
                                                                                                  ctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAcc                                               <  2:719641/149‑1 (MQ=255)
                                                                                                    gcgcTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCt                                             <  1:2345209/149‑1 (MQ=255)
                                                                                                      gcTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTAc                                           >  2:442420/1‑149 (MQ=255)
                                                                                                         gTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGcc                                        <  1:4730108/149‑1 (MQ=255)
                                                                                                         gTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGcc                                        <  1:447289/149‑1 (MQ=255)
                                                                                                          tATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                       <  2:4783947/149‑1 (MQ=255)
                                                                                                              cGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGctc                                   <  1:2233525/149‑1 (MQ=255)
                                                                                                               gCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGctct                                  <  1:1077537/149‑1 (MQ=255)
                                                                                                               gCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGctct                                  >  2:3568142/1‑149 (MQ=255)
                                                                                                               gCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGctct                                  <  2:1873434/149‑1 (MQ=255)
                                                                                                                cTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCtt                                 <  1:3534342/149‑1 (MQ=255)
                                                                                                                  gCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTa                               >  1:1757037/1‑149 (MQ=255)
                                                                                                                  gCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTa                               >  1:4146940/1‑149 (MQ=255)
                                                                                                                    ggggCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATg                             >  2:4372152/1‑149 (MQ=255)
                                                                                                                    ggggCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATg                             <  2:3037555/149‑1 (MQ=255)
                                                                                                                     gggCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGc                            >  1:3293256/1‑149 (MQ=255)
                                                                                                                      ggCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                           >  1:3373119/1‑149 (MQ=255)
                                                                                                                       gCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGcac                          <  2:1579030/149‑1 (MQ=255)
                                                                                                                       gCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGcac                          <  2:4532027/149‑1 (MQ=255)
                                                                                                                         ttGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAg                        >  1:2145905/1‑149 (MQ=255)
                                                                                                                          tGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGc                       <  1:3590898/149‑1 (MQ=255)
                                                                                                                          tGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGc                       >  2:3612359/1‑149 (MQ=255)
                                                                                                                            tATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCtt                     >  2:1254077/1‑149 (MQ=255)
                                                                                                                             aTGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtg                    <  2:4789172/149‑1 (MQ=255)
                                                                                                                              tGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtgt                   >  1:1657916/1‑149 (MQ=255)
                                                                                                                               gAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtgtg                  >  2:4643811/1‑149 (MQ=255)
                                                                                                                                   aCTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACaa              <  1:4055031/149‑1 (MQ=255)
                                                                                                                                       gTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtg          >  2:1758046/1‑149 (MQ=255)
                                                                                                                                       gTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtg          <  2:4574552/149‑1 (MQ=255)
                                                                                                                                         ccGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgtg        <  1:1280275/149‑1 (MQ=255)
                                                                                                                                          cGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgtgt       <  1:3468261/149‑1 (MQ=255)
                                                                                                                                          cGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgtgt       >  1:85836/1‑149 (MQ=255)
                                                                                                                                           ggCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTc      >  2:2171541/1‑149 (MQ=255)
                                                                                                                                            gCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTcc     >  1:3914070/1‑149 (MQ=255)
                                                                                                                                              atatAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCgg   >  1:1525825/1‑149 (MQ=255)
                                                                                                                                               tataGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGt  <  2:415330/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGT  >  CP009273/318878‑319169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑