Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,879,935 C→A 5.9% V500V (GTC→GTA)  yidC → membrane protein insertase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,879,9350CA 5.9% 1033.1 / 23.7 267V500V (GTC→GTA) yidCmembrane protein insertase
Reads supporting (aligned to +/- strand):  ref base C (143/107);  new base A (8/9);  total (151/116)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

TTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGAT  >  CP009273/3879787‑3880083
                                                                                                                                                    |                                                                                                                                                    
ttGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTc                                                                                                                                                      <  2:4747736/149‑1 (MQ=255)
 tGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCa                                                                                                                                                     <  1:706133/149‑1 (MQ=255)
 tGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCa                                                                                                                                                     <  2:3095295/149‑1 (MQ=255)
 tGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCa                                                                                                                                                     <  1:2478491/149‑1 (MQ=255)
      tgtgGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTc                                                                                                                                                >  2:3224662/1‑149 (MQ=255)
       gtgGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCa                                                                                                                                               >  1:1387035/1‑149 (MQ=255)
       gtgGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCa                                                                                                                                               >  2:4570047/1‑149 (MQ=255)
           aTCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTAACCgt                                                                                                                                           >  2:1003015/1‑149 (MQ=255)
             ccACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCgtgt                                                                                                                                         <  2:4455646/149‑1 (MQ=255)
               aCGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGttc                                                                                                                                       >  1:1040102/1‑149 (MQ=255)
               aCGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGttc                                                                                                                                       <  2:1589367/149‑1 (MQ=255)
               aCGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGttc                                                                                                                                       >  1:4551369/1‑149 (MQ=255)
               aCGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGttc                                                                                                                                       >  1:3429937/1‑149 (MQ=255)
               aCGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGttc                                                                                                                                       >  1:3415407/1‑149 (MQ=255)
               aCGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGttc                                                                                                                                       >  1:1040052/1‑149 (MQ=255)
                  aCCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCATTATGCCGGTAATCTTCACCGTGttcttc                                                                                                                                    >  1:4748589/1‑149 (MQ=255)
                      gTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCtgt                                                                                                                                <  1:825149/149‑1 (MQ=255)
                      gTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCtgt                                                                                                                                >  1:2018546/1‑149 (MQ=255)
                         ggCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGt                                                                                                                             >  2:285702/1‑149 (MQ=255)
                            acaGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTcc                                                                                                                          >  1:619096/1‑149 (MQ=255)
                            acaGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCATTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTcc                                                                                                                          <  2:4292469/149‑1 (MQ=255)
                                gACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTc                                                                                                                      >  2:3710095/1‑149 (MQ=255)
                                  cccGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAg                                                                                                                    <  1:4224491/149‑1 (MQ=255)
                                     gTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTc                                                                                                                 <  1:3718423/149‑1 (MQ=255)
                                      tactacATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCt                                                                                                                >  2:4428233/1‑149 (MQ=255)
                                      tactacATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCt                                                                                                                >  2:365826/1‑149 (MQ=255)
                                       actacATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTg                                                                                                               <  2:1846458/149‑1 (MQ=255)
                                        ctacATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTgg                                                                                                              >  2:2746877/1‑149 (MQ=255)
                                        ctacATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTAATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTgg                                                                                                              <  1:4335912/149‑1 (MQ=255)
                                             tCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTg                                                                                                         >  2:4490632/1‑149 (MQ=255)
                                               cTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTa                                                                                                       >  1:3944497/1‑149 (MQ=255)
                                                  ccGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACta                                                                                                    <  2:1351368/149‑1 (MQ=255)
                                                  ccGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACta                                                                                                    <  1:3582272/149‑1 (MQ=255)
                                                     aTCCGGATGGGAGTAACGTTGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGTTGCAGCAGAAGATCATGACCTTTAGGCCTGTCATCTTCACCGTGTTCTTCGTGTGGTTCCCGTAAGGTCTGGTGCTGTACTatat                                                                                                 <  2:3998196/149‑1 (MQ=255)
                                                       ccTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCg                                                                                               <  2:4671634/149‑1 (MQ=255)
                                                          gATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCa                                                                                            >  1:3160715/1‑149 (MQ=255)
                                                          gATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCa                                                                                            >  1:4186045/1‑149 (MQ=255)
                                                           aTGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAg                                                                                           >  2:667605/1‑149 (MQ=255)
                                                            tGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGc                                                                                          >  2:747721/1‑149 (MQ=255)
                                                               gCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTAATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAAc                                                                                       <  2:3308519/149‑1 (MQ=255)
                                                                cGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAAcc                                                                                      <  1:357483/149‑1 (MQ=255)
                                                                  tAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTg                                                                                    >  1:185726/1‑149 (MQ=255)
                                                                   aaCGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGTCCTTTATGCCGGTCTTCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTgg                                                                                   <  2:3919618/149‑1 (MQ=255)
                                                                      gATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTaa                                                                                <  1:4592483/149‑1 (MQ=255)
                                                                      gATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTaa                                                                                <  2:2462040/149‑1 (MQ=255)
                                                                       aTGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAAc                                                                               >  1:2252344/1‑149 (MQ=255)
                                                                        tGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAAcc                                                                              <  1:2407370/149‑1 (MQ=255)
                                                                        tGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAAcc                                                                              <  1:928608/149‑1 (MQ=255)
                                                                         gTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCa                                                                             <  2:2882586/149‑1 (MQ=255)
                                                                           tcttcATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCatt                                                                           <  2:828987/149‑1 (MQ=255)
                                                                            cttcATTCAGAAGATGTCGCCGACCACAGTGACCGGCCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCatta                                                                          >  1:548296/1‑149 (MQ=255)
                                                                             ttcattcaGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCattat                                                                         <  1:3221280/149‑1 (MQ=255)
                                                                               cattcaGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTc                                                                       >  2:4105791/1‑149 (MQ=255)
                                                                               cattcaGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTc                                                                       <  2:4736772/149‑1 (MQ=255)
                                                                                attcaGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTca                                                                      <  1:4109324/149‑1 (MQ=255)
                                                                                attcaGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTca                                                                      >  2:2162644/1‑149 (MQ=255)
                                                                                  tcaGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTcagc                                                                    >  2:208029/1‑149 (MQ=255)
                                                                                        gaTGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCagcagcagc                                                              <  2:1337708/149‑1 (MQ=255)
                                                                                        gaTGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCagcagcagc                                                              <  1:4463829/149‑1 (MQ=255)
                                                                                         aTGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCt                                                             >  2:643913/1‑149 (MQ=255)
                                                                                            tCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGAt                                                          <  1:295575/149‑1 (MQ=255)
                                                                                            tCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTAATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGAt                                                          <  2:1894344/149‑1 (MQ=255)
                                                                                               ccGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTa                                                       >  2:4753086/1‑149 (MQ=255)
                                                                                                cGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTAc                                                      <  2:4599751/149‑1 (MQ=255)
                                                                                                cGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTAc                                                      >  1:97614/1‑149 (MQ=255)
                                                                                                   ccACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGTTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGt                                                   >  2:3676466/1‑149 (MQ=255)
                                                                                                     acaGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTgg                                                 >  1:2895311/1‑149 (MQ=255)
                                                                                                      caGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGt                                                <  1:672116/149‑1 (MQ=255)
                                                                                                      caGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGt                                                >  1:25586/1‑149 (MQ=255)
                                                                                                      caGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATGTACCGTGGt                                                >  1:25495/1‑149 (MQ=255)
                                                                                                       aGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTc                                               >  1:40524/1‑149 (MQ=255)
                                                                                                        gTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCt                                              >  2:3454911/1‑149 (MQ=255)
                                                                                                          gaccgaccCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTgg                                            >  2:3540843/1‑149 (MQ=255)
                                                                                                               accCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGaaaaa                                       >  2:556215/1‑149 (MQ=255)
                                                                                                                cccGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAAc                                      <  1:2745851/149‑1 (MQ=255)
                                                                                                                 ccGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACg                                     >  1:3880745/1‑149 (MQ=255)
                                                                                                                   gATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTg                                   <  2:3826371/149‑1 (MQ=255)
                                                                                                                    aTGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTgg                                  <  1:2399258/149‑1 (MQ=255)
                                                                                                                           agaagaTCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCAt                           <  2:1387035/149‑1 (MQ=255)
                                                                                                                            gaagaTCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATa                          <  1:3854642/149‑1 (MQ=255)
                                                                                                                             aagaTCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAg                         >  2:339025/1‑149 (MQ=255)
                                                                                                                              agaTCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGc                        >  1:353113/1‑149 (MQ=255)
                                                                                                                              agaTCATGACCTTTATGCCGGTAATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGc                        >  1:2291176/1‑149 (MQ=255)
                                                                                                                               gaTCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGcc                       >  1:1345348/1‑149 (MQ=255)
                                                                                                                               gaTCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGcc                       <  2:1973014/149‑1 (MQ=255)
                                                                                                                               gaTCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGcc                       <  2:2539128/149‑1 (MQ=255)
                                                                                                                                aTCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAATCGTGGCCTGCATAGCcg                      >  2:1188989/1‑149 (MQ=255)
                                                                                                                                     gACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCgaga                 >  2:1579434/1‑149 (MQ=255)
                                                                                                                                       ccTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGagaag               >  2:2986431/1‑149 (MQ=255)
                                                                                                                                           tATGCCGGTCATATTCTCCGTGTTCTTCCTGTGGTTCCGGTCTGGTCTGGTGCTGTTCTATATCGTCAGCTACCTGGTTACGATTTTTCAGCAGCAGCTGATTTACGGTGGTCTTTAAATTCGTTGGCTGCTTTGCCGGTAGACGaaaa           <  2:2214462/149‑1 (MQ=255)
                                                                                                                                            aTGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGaaaaa          >  2:161205/1‑149 (MQ=255)
                                                                                                                                            aTGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGaaaaa          >  1:1505453/1‑149 (MQ=255)
                                                                                                                                             tGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGaaaaaa         >  2:509122/1‑149 (MQ=255)
                                                                                                                                               ccGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATc       >  2:2790712/1‑149 (MQ=255)
                                                                                                                                                 ggTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTATTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCt     <  1:3475669/149‑1 (MQ=255)
                                                                                                                                                 ggTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCt     <  1:517271/149‑1 (MQ=255)
                                                                                                                                                 ggTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCt     >  2:1828842/1‑149 (MQ=255)
                                                                                                                                                 ggTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCt     >  1:1632720/1‑149 (MQ=255)
                                                                                                                                                 ggTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACAATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCt     >  1:2645402/1‑149 (MQ=255)
                                                                                                                                                   tCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGa   <  1:1416848/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACCTTTATGCCGGTCATCTTCACCGTGTTCTTCCTGTGGTTCCCGTCAGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGAT  >  CP009273/3879787‑3880083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑