BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,893,713	C→A	7.7%	P75P (CCG→CCT)	yieL ←	putative xylanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,893,713	0	C	A	7.7%	1063.3 / 36.8	288	P75P (CCG→CCT)	yieL	putative xylanase
Reads supporting (aligned to +/- strand): ref base C (135/130); new base A (17/6); total (152/136)
Fisher's exact test for biased strand distribution p-value = 4.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

TACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCA  >  CP009273/3893565‑3893861

tACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATc                                                                                                                                                      <  1:2155640/149‑1 (MQ=255)

  cTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCgg                                                                                                                                                    >  2:2801805/1‑149 (MQ=255)

   tGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCggg                                                                                                                                                   <  2:3476091/149‑1 (MQ=255)

    ggAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGt                                                                                                                                                  <  2:3700140/149‑1 (MQ=255)

    ggAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGt                                                                                                                                                  <  1:4202590/149‑1 (MQ=255)

     gAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTg                                                                                                                                                 >  2:1083091/1‑149 (MQ=255)

         tAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAata                                                                                                                                             <  2:3526827/149‑1 (MQ=255)

          aaCCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAatat                                                                                                                                            >  2:2726382/1‑149 (MQ=255)

             caggCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGt                                                                                                                                         >  1:639983/3‑149 (MQ=255)

              tGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGGACACCATCAACATTGAAAAAATACTCGTACAGGGTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTc                                                                                                                                        <  2:199028/149‑1 (MQ=255)

               ggCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATAGGGTGAATATTGTcc                                                                                                                                       >  2:2613278/1‑149 (MQ=255)

                gcgcTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccg                                                                                                                                      <  1:2043092/149‑1 (MQ=255)

                    tGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccggaac                                                                                                                                  <  2:2311332/149‑1 (MQ=255)

                    tGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccggaac                                                                                                                                  <  2:2270120/149‑1 (MQ=255)

                     gAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccggaacc                                                                                                                                 <  2:3193142/149‑1 (MQ=255)

                     gAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccggaacc                                                                                                                                 >  2:2484605/1‑149 (MQ=255)

                      aGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccggaaccg                                                                                                                                <  2:2932595/149‑1 (MQ=255)

                       ggATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATAGGGTGAATATTGTccggaaccgg                                                                                                                               >  1:426465/1‑149 (MQ=255)

                        gATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTccggaaccgga                                                                                                                              >  2:3148055/1‑149 (MQ=255)

                            aGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAacac                                                                                                                          >  1:702344/1‑149 (MQ=255)

                                 ttGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCCGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAAcacccacc                                                                                                                     >  2:4160788/1‑149 (MQ=255)

                                 ttGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATAGGGTGAATATTGTCCGGAACCGGAAcacccacc                                                                                                                     >  2:3621450/1‑149 (MQ=255)

                                   gCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCcaccac                                                                                                                   <  2:4704508/149‑1 (MQ=255)

                                   gCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCcaccac                                                                                                                   <  2:838513/149‑1 (MQ=255)

                                    cGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACt                                                                                                                  <  2:870029/149‑1 (MQ=255)

                                         ccTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACaga                                                                                                             >  1:3720349/1‑149 (MQ=255)

                                         ccTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACaga                                                                                                             <  1:2647703/149‑1 (MQ=255)

                                          cTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACagag                                                                                                            >  1:347401/1‑149 (MQ=255)

                                          cTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACagag                                                                                                            >  1:3237648/1‑149 (MQ=255)

                                           tGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAgaga                                                                                                           <  1:2236406/149‑1 (MQ=255)

                                           tGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAgaga                                                                                                           <  1:4633545/149‑1 (MQ=255)

                                            gTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGAc                                                                                                          >  1:101720/1‑149 (MQ=255)

                                             tATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACa                                                                                                         >  2:1746218/1‑149 (MQ=255)

                                             tATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACa                                                                                                         >  1:3018028/1‑149 (MQ=255)

                                               tCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACAtt                                                                                                       >  1:1044909/1‑149 (MQ=255)

                                               tCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACAtt                                                                                                       >  2:3005420/1‑149 (MQ=255)

                                               tCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACAtt                                                                                                       <  2:4638839/149‑1 (MQ=255)

                                                 aaTGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATAGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACAtttt                                                                                                     <  2:4668942/149‑1 (MQ=255)

                                                  aTGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACAttttt                                                                                                    >  1:3774794/1‑149 (MQ=255)

                                                   tgctgcGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGACTATTGTCCGGAACCGGAACACCCACCACTACAGAGACAtttttt                                                                                                   >  2:4779263/1‑149 (MQ=255)

                                                   tgctgcGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACAtttttt                                                                                                   >  2:3848432/1‑149 (MQ=255)

                                                     ctgcGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGc                                                                                                 <  2:676895/149‑1 (MQ=255)

                                                       gcGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGccc                                                                                               >  1:4171815/1‑149 (MQ=255)

                                                          tACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAg                                                                                            >  1:4014218/1‑149 (MQ=255)

                                                             acCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAgggg                                                                                         <  2:3135883/149‑1 (MQ=255)

                                                              ccATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGc                                                                                        >  2:3568019/1‑149 (MQ=255)

                                                               cATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCa                                                                                       >  2:4212455/1‑149 (MQ=255)

                                                                aTCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCaa                                                                                      >  2:4452971/1‑149 (MQ=255)

                                                                aTCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCaa                                                                                      >  1:1788467/1‑149 (MQ=255)

                                                                 tCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATAGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCaaa                                                                                     >  2:3749774/1‑149 (MQ=255)

                                                                    aCATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTa                                                                                  <  1:1100534/149‑1 (MQ=255)

                                                                    aCATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATAGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTa                                                                                  <  2:1242903/149‑1 (MQ=255)

                                                                     cATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAg                                                                                 <  1:3586633/149‑1 (MQ=255)

                                                                      aTTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGc                                                                                <  1:1770251/149‑1 (MQ=255)

                                                                         gAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGaa                                                                             <  2:4435118/149‑1 (MQ=255)

                                                                         gAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGaa                                                                             <  2:587200/149‑1 (MQ=255)

                                                                          aaaaaaTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGaaa                                                                            <  2:1922729/149‑1 (MQ=255)

                                                                           aaaaaTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAg                                                                           <  2:266893/149‑1 (MQ=255)

                                                                            aaaaGACTCGGACCGGTTGCCTGTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATAGTGGCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAgg                                                                          <  2:3800527/149‑1 (MQ=255)

                                                                               aTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCa                                                                       <  2:1397256/149‑1 (MQ=255)

                                                                               aTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCa                                                                       >  2:3249359/1‑149 (MQ=255)

                                                                                tACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCAc                                                                      <  1:2753767/149‑1 (MQ=255)

                                                                                     gTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGt                                                                 >  2:4512943/1‑149 (MQ=255)

                                                                                      tACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGtt                                                                <  1:2910652/149‑1 (MQ=255)

                                                                                      tACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGtt                                                                >  1:3161226/1‑149 (MQ=255)

                                                                                      tACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGtt                                                                <  2:4732698/149‑1 (MQ=255)

                                                                                      tACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGtt                                                                >  1:1496345/1‑149 (MQ=255)

                                                                                        cAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTAt                                                              <  2:4197183/149‑1 (MQ=255)

                                                                                        cAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTAt                                                              <  2:4529519/149‑1 (MQ=255)

                                                                                        cAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTAt                                                              <  2:1253568/149‑1 (MQ=255)

                                                                                         aGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATc                                                             >  2:2424173/1‑149 (MQ=255)

                                                                                         aGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATc                                                             <  1:610064/149‑1 (MQ=255)

                                                                                             tGCCCTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCa                                                         <  2:1930325/149‑1 (MQ=255)

                                                                                              gCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCAt                                                        >  1:1435192/1‑149 (MQ=255)

                                                                                              gCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCAt                                                        >  1:1435028/1‑149 (MQ=255)

                                                                                                cTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTg                                                      >  2:1723097/1‑149 (MQ=255)

                                                                                                 tttCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGa                                                     >  2:4045375/1‑149 (MQ=255)

                                                                                                  ttCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGAc                                                    <  1:2484605/149‑1 (MQ=255)

                                                                                                    cAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCt                                                  <  1:2303361/149‑1 (MQ=255)

                                                                                                    cAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCt                                                  >  2:2207266/1‑149 (MQ=255)

                                                                                                      ggATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGa                                                >  1:4279547/1‑149 (MQ=255)

                                                                                                           ggTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTcac                                           >  1:536363/1‑149 (MQ=255)

                                                                                                             tgtgCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATAGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACAt                                         >  2:2889641/1‑149 (MQ=255)

                                                                                                                 cgcCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCGGTTATCGGCATTGACCTGAGTCACATACTg                                     >  1:1770477/1‑149 (MQ=255)

                                                                                                                        aCCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCAcc                              >  1:96952/1‑149 (MQ=255)

                                                                                                                            gACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGa                          >  2:2530485/1‑149 (MQ=255)

                                                                                                                            gACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGa                          <  2:698525/149‑1 (MQ=255)

                                                                                                                             aCTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGAt                         <  1:4322460/149‑1 (MQ=255)

                                                                                                                              cTCCTGCTTCGTCTTTGGTCATAGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATa                        >  1:4675488/1‑149 (MQ=255)

                                                                                                                                  tGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTgg                    >  2:3315681/1‑149 (MQ=255)

                                                                                                                                   gCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTggg                   <  2:1121853/149‑1 (MQ=255)

                                                                                                                                   gCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTggg                   <  2:4724138/149‑1 (MQ=255)

                                                                                                                                       cGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGcc               <  1:3359094/149‑1 (MQ=255)

                                                                                                                                        gTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCg              >  2:1547033/1‑149 (MQ=255)

                                                                                                                                        gTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCg              >  2:1109436/1‑149 (MQ=255)

                                                                                                                                           tttGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggc           <  2:2380151/149‑1 (MQ=255)

                                                                                                                                               gTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggctggc       <  1:4515759/149‑1 (MQ=255)

                                                                                                                                                    cGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCa  <  1:4072416/149‑1 (MQ=255)

                                                                                                                                                    cGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCa  <  1:3455553/149‑1 (MQ=255)

                                                                                                                                                    cGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTGGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCAGTGACCTGAGTCACATACTGGTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCa  <  1:1682819/149‑1 (MQ=255)

TACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCA  >  CP009273/3893565‑3893861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`