Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,910,894 C→A 100% M244I (ATG→ATT)  atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,910,8940CA99.6% 1033.4 / ‑4.4 302M244I (ATG→ATT) atpGF1 sector of membrane‑bound ATP synthase, gamma subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (156/144);  total (157/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 305 total aligned reads displayed.

ACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCA  >  CP009273/3910748‑3911038
                                                                                                                                                  |                                                                                                                                                
aCGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAAt                                                                                                                                                <  2:3932835/149‑1 (MQ=255)
  gAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATAc                                                                                                                                              >  1:84816/1‑149 (MQ=255)
   aaaTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACg                                                                                                                                             >  2:2036373/1‑149 (MQ=255)
   aaaTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACg                                                                                                                                             >  2:730530/1‑149 (MQ=255)
    aaTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATCCCAACGGCAGCTCTATAATCAGGCTGCCGCCATAGTCGGTCGCGGATTTCATCGCCACAATACgg                                                                                                                                            >  2:331739/1‑149 (MQ=255)
     aTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACggg                                                                                                                                           >  1:1401990/1‑149 (MQ=255)
         ccTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGggcggc                                                                                                                                       >  2:746911/1‑149 (MQ=255)
          cTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGcc                                                                                                                                      >  1:245227/1‑149 (MQ=255)
           tGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCt                                                                                                                                     >  1:3609374/1‑149 (MQ=255)
           tGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCt                                                                                                                                     >  1:4663475/1‑149 (MQ=255)
               tAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTc                                                                                                                                 <  2:3391551/149‑1 (MQ=255)
                 aaCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGc                                                                                                                               >  1:1044465/1‑149 (MQ=255)
                  aCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCt                                                                                                                              >  1:3341525/1‑149 (MQ=255)
                  aCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCt                                                                                                                              >  1:2865288/1‑149 (MQ=255)
                      cggcggCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGcc                                                                                                                          <  2:4725064/149‑1 (MQ=255)
                        gcggcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAg                                                                                                                        <  2:271214/149‑1 (MQ=255)
                          ggcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGt                                                                                                                      <  2:3853032/149‑1 (MQ=255)
                           gcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGtt                                                                                                                     <  2:1035686/149‑1 (MQ=255)
                                 gagaCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAAc                                                                                                               >  2:2199891/1‑149 (MQ=255)
                                  agaCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAAcc                                                                                                              >  2:3218368/1‑149 (MQ=255)
                                  agaCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAAcc                                                                                                              <  2:3074606/149‑1 (MQ=255)
                                   gaCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCa                                                                                                             >  1:3745849/1‑149 (MQ=255)
                                   gaCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCa                                                                                                             >  2:1933657/1‑149 (MQ=255)
                                   gaCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCa                                                                                                             <  2:2421987/149‑1 (MQ=255)
                                    aCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCAc                                                                                                            >  1:730995/1‑149 (MQ=255)
                                     cGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACg                                                                                                           <  1:4774584/149‑1 (MQ=255)
                                             gTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATa                                                                                                   <  1:41598/149‑1 (MQ=255)
                                              tGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATaa                                                                                                  >  2:2785158/1‑149 (MQ=255)
                                               gAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATaaa                                                                                                 >  1:2649288/1‑149 (MQ=255)
                                               gAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATaaa                                                                                                 <  2:1342233/149‑1 (MQ=255)
                                               gAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATaaa                                                                                                 <  2:1563962/149‑1 (MQ=255)
                                                 gTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAAcc                                                                                               >  1:1684871/1‑149 (MQ=255)
                                                   tCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTg                                                                                             >  1:356017/1‑149 (MQ=255)
                                                      tGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTgaga                                                                                          <  2:893673/149‑1 (MQ=255)
                                                      tGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTgaga                                                                                          >  2:43793/1‑149 (MQ=255)
                                                      tGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTgaga                                                                                          >  2:2870292/1‑149 (MQ=255)
                                                        aGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGAtt                                                                                        <  1:1147177/149‑1 (MQ=255)
                                                         gTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTc                                                                                       >  1:329011/1‑149 (MQ=255)
                                                           aaTGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGa                                                                                     <  1:3767913/149‑1 (MQ=255)
                                                           aaTGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGa                                                                                     >  1:1848591/1‑149 (MQ=255)
                                                            aTGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGAc                                                                                    >  1:1531350/1‑149 (MQ=255)
                                                            aTGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGAc                                                                                    <  2:245277/149‑1 (MQ=255)
                                                            aTGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGAc                                                                                    <  2:3523622/149‑1 (MQ=255)
                                                             tGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACa                                                                                   <  1:2112641/149‑1 (MQ=255)
                                                                 ggCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAac                                                                               >  1:2580834/1‑149 (MQ=255)
                                                                 ggCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAac                                                                               >  2:4758782/1‑149 (MQ=255)
                                                                   ccTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAacga                                                                             <  1:4763195/149‑1 (MQ=255)
                                                                    cTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAacgac                                                                            <  1:1473406/149‑1 (MQ=255)
                                                                       aCGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACgca                                                                         <  1:1618937/149‑1 (MQ=255)
                                                                        cGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCAGTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACgcag                                                                        >  2:455532/1‑149 (MQ=255)
                                                                         gAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACgcagc                                                                       >  1:1739101/1‑149 (MQ=255)
                                                                           gCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGcagcag                                                                     >  2:1676056/1‑149 (MQ=255)
                                                                           gCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGcagcag                                                                     <  1:636145/149‑1 (MQ=255)
                                                                           gCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGcagcag                                                                     >  1:3245017/1‑149 (MQ=255)
                                                                             tttGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTACTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAggg                                                                   <  1:91574/149‑1 (MQ=255)
                                                                                  tgtATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATcc                                                              <  1:973878/149‑1 (MQ=255)
                                                                                   gtATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCa                                                             <  1:3006486/149‑1 (MQ=255)
                                                                                   gtATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCa                                                             <  2:3754039/149‑1 (MQ=255)
                                                                                      taCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACCTAACGACGCAGCAGGGTATCCAGCa                                                          >  2:1047180/1‑149 (MQ=255)
                                                                                       aCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCaa                                                         >  1:3874936/1‑149 (MQ=255)
                                                                                       aCCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCaa                                                         <  1:2772953/149‑1 (MQ=255)
                                                                                        ccAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAAc                                                        >  1:2229393/1‑149 (MQ=255)
                                                                                        ccAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAAc                                                        >  1:4370370/1‑149 (MQ=255)
                                                                                           aCTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGcc                                                     <  1:892612/149‑1 (MQ=255)
                                                                                           aCTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGcc                                                     <  1:1073936/149‑1 (MQ=255)
                                                                                               cAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCg                                                 >  2:4141005/1‑149 (MQ=255)
                                                                                                aGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCgg                                                >  1:1529864/1‑149 (MQ=255)
                                                                                                aGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCgg                                                >  1:1924491/1‑149 (MQ=255)
                                                                                                aGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCgg                                                >  1:2352597/1‑149 (MQ=255)
                                                                                                 gCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGa                                               >  2:1141574/1‑149 (MQ=255)
                                                                                                  ctctTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGAt                                              >  2:2436237/1‑149 (MQ=255)
                                                                                                   tctTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATc                                             >  2:1782968/1‑149 (MQ=255)
                                                                                                       tAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGt                                         <  1:1823798/149‑1 (MQ=255)
                                                                                                        aaTCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGtt                                        >  2:801125/1‑149 (MQ=255)
                                                                                                        aaTCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGtt                                        <  2:3642198/149‑1 (MQ=255)
                                                                                                         aTCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTc                                       >  1:2171512/1‑149 (MQ=255)
                                                                                                            aGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTa                                    >  1:4138639/1‑149 (MQ=255)
                                                                                                            aGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTa                                    >  1:2616980/1‑149 (MQ=255)
                                                                                                              gCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACa                                  >  2:591274/1‑149 (MQ=255)
                                                                                                              gCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACa                                  >  2:162803/1‑149 (MQ=255)
                                                                                                              gCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACa                                  <  2:3427380/149‑1 (MQ=255)
                                                                                                                 gccgccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGt                               <  2:466653/149‑1 (MQ=255)
                                                                                                                  ccgccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTa                              >  1:3161447/1‑149 (MQ=255)
                                                                                                                   cgccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTaa                             <  2:83845/149‑1 (MQ=255)
                                                                                                                    gccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAAt                            <  1:805867/149‑1 (MQ=255)
                                                                                                                     ccATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATc                           >  1:4288222/1‑149 (MQ=255)
                                                                                                                      cATTGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATcc                          <  2:1160119/149‑1 (MQ=255)
                                                                                                                       aTTGTCGGTCGCGGCTTTCATCGCCACAAGACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATccc                         <  2:2832133/149‑1 (MQ=255)
                                                                                                                         tGTCGGTCGCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAg                       <  1:4251398/149‑1 (MQ=255)
                                                                                                                           tcggtcgCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGa                     >  2:3145848/1‑149 (MQ=255)
                                                                                                                             ggtcgCGGCTTTCATCGCCACAAGACGGGCGGCCTGCTCGCTGGCCAGGATTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGAtt                   <  2:2590045/149‑1 (MQ=255)
                                                                                                                              gtcgCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGAttt                  >  2:814711/1‑149 (MQ=255)
                                                                                                                              gtcgCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGAttt                  <  2:2847490/149‑1 (MQ=255)
                                                                                                                              gtcgCGGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGAttt                  <  2:4226181/149‑1 (MQ=255)
                                                                                                                                cgcgGCTTTCATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTAt                <  1:1331934/149‑1 (MQ=255)
                                                                                                                                         cATCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGat       >  2:1889729/1‑149 (MQ=255)
                                                                                                                                           tCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatca     >  1:1645430/1‑149 (MQ=255)
                                                                                                                                           tCGCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatca     >  1:4033098/1‑149 (MQ=255)
                                                                                                                                             gCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGatcatc   >  1:1813122/1‑149 (MQ=255)
                                                                                                                                             gCCACAATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGAGTTATGTTTCAGatcatc   >  1:3392780/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTCAACCACGCCCTGATAAACCTGAGATTCGACATAACGACGCAGCAGGGTATCCAGCAACGCCTTCGGATCGGGTTCGTACAGGTAATCCCAGGATTTATGTTTCAGATCATCA  >  CP009273/3910748‑3911038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑