Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,932,671 G→T 5.1% I434I (ATC→ATA)  hsrA ← putative multidrug or homocysteine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,932,6710GT 5.1% 1083.5 / 20.7 278I434I (ATC→ATA) hsrAputative multidrug or homocysteine efflux system
Reads supporting (aligned to +/- strand):  ref base G (149/114);  new base T (7/8);  total (156/122)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

TTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCAT  >  CP009273/3932524‑3932819
                                                                                                                                                   |                                                                                                                                                    
ttCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                                                                                                                                     <  1:4738468/149‑1 (MQ=255)
ttCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                                                                                                                                     >  1:541305/1‑149 (MQ=255)
ttCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                                                                                                                                     <  1:1969938/149‑1 (MQ=255)
 tCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGAt                                                                                                                                                    <  2:2653892/149‑1 (MQ=255)
  cAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATa                                                                                                                                                   <  2:787907/149‑1 (MQ=255)
    gCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATaaa                                                                                                                                                 <  1:1481364/149‑1 (MQ=255)
    gCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATaaa                                                                                                                                                 <  1:2540834/149‑1 (MQ=255)
     caacaaCGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAAc                                                                                                                                                <  2:2872060/149‑1 (MQ=255)
     caacaaCGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAAc                                                                                                                                                <  2:1088525/149‑1 (MQ=255)
      aacaacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                                                                                                                                               >  1:799735/1‑149 (MQ=255)
         aacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGtat                                                                                                                                            >  1:2153545/1‑149 (MQ=255)
          acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGtata                                                                                                                                           >  1:4311119/1‑149 (MQ=255)
            gATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGt                                                                                                                                         <  1:3066896/149‑1 (MQ=255)
            gATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGt                                                                                                                                         <  1:1333248/149‑1 (MQ=255)
              ttACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTgg                                                                                                                                       >  2:598277/1‑149 (MQ=255)
                 cAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAAt                                                                                                                                    >  1:3846379/1‑149 (MQ=255)
                   aCTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTg                                                                                                                                  <  2:872637/149‑1 (MQ=255)
                    cTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGt                                                                                                                                 >  1:2371258/1‑149 (MQ=255)
                     ttACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGtt                                                                                                                                >  2:4469178/1‑149 (MQ=255)
                       aCTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCg                                                                                                                              <  2:3074531/149‑1 (MQ=255)
                        cTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGa                                                                                                                             >  2:475852/1‑149 (MQ=255)
                          ccGAGTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTTATAAACGTATAGTGGAATTGTTCGACa                                                                                                                           <  2:4686735/149‑1 (MQ=255)
                             aTTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTTATAAACGTATAGTGGAATTGTTCGACAGTc                                                                                                                        >  1:2793534/1‑149 (MQ=255)
                             aTTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTc                                                                                                                        >  1:1862655/1‑149 (MQ=255)
                             aTTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTc                                                                                                                        >  2:3568023/1‑149 (MQ=255)
                              ttCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCg                                                                                                                       >  2:2116902/1‑149 (MQ=255)
                              ttCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCg                                                                                                                       >  2:4652078/1‑149 (MQ=255)
                              ttCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCg                                                                                                                       >  2:2254783/1‑149 (MQ=255)
                               tCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGt                                                                                                                      <  1:664629/149‑1 (MQ=255)
                                cTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGtt                                                                                                                     <  1:428064/149‑1 (MQ=255)
                                 tGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtg                                                                                                                    >  1:4513178/1‑149 (MQ=255)
                                   aTGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtgtg                                                                                                                  >  2:3112731/1‑149 (MQ=255)
                                    tGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGc                                                                                                                 <  2:3989885/149‑1 (MQ=255)
                                     ggAACGCGGTTCGGCGTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGcc                                                                                                                >  1:2189881/1‑149 (MQ=255)
                                      gAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCt                                                                                                               <  2:4023794/149‑1 (MQ=255)
                                       aaCGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGccgt                                                                                                              >  2:1523270/1‑147 (MQ=255)
                                        aCGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTc                                                                                                             <  2:1828493/149‑1 (MQ=255)
                                        aCGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTc                                                                                                             <  1:4450601/149‑1 (MQ=255)
                                           cgGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCAt                                                                                                          <  2:4311119/149‑1 (MQ=255)
                                           cgGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCAt                                                                                                          <  1:3632027/149‑1 (MQ=255)
                                            ggTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCAtt                                                                                                         >  1:4257350/1‑149 (MQ=255)
                                             gTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTc                                                                                                        >  1:4151635/1‑149 (MQ=255)
                                              ttCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTcc                                                                                                       >  1:308324/1‑149 (MQ=255)
                                                cGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCtt                                                                                                     <  2:2600422/149‑1 (MQ=255)
                                                 ggCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCATTCCATTACTTc                                                                                                    >  2:3381167/1‑149 (MQ=255)
                                                  gCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCa                                                                                                   <  1:4373027/149‑1 (MQ=255)
                                                  gCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCa                                                                                                   >  1:2333836/1‑149 (MQ=255)
                                                   cTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCAt                                                                                                  >  1:4543215/1‑149 (MQ=255)
                                                    ttAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATa                                                                                                 >  1:670398/1‑149 (MQ=255)
                                                    ttAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATa                                                                                                 >  2:2829730/1‑149 (MQ=255)
                                                          ttACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAAcgcg                                                                                           >  1:1803991/1‑149 (MQ=255)
                                                            aCGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGaa                                                                                         >  2:104940/1‑149 (MQ=255)
                                                            aCGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGaa                                                                                         >  2:1888855/1‑149 (MQ=255)
                                                               cTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGa                                                                                      >  2:1079361/1‑149 (MQ=255)
                                                                tGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGAc                                                                                     <  2:1213904/149‑1 (MQ=255)
                                                                  tCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACgg                                                                                   >  2:2702775/1‑149 (MQ=255)
                                                                          tCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACtt                                                                           >  1:3386472/1‑149 (MQ=255)
                                                                          tCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACtt                                                                           <  1:601406/149‑1 (MQ=255)
                                                                                tattaCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCa                                                                     >  1:2515410/1‑149 (MQ=255)
                                                                                 attaCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTTATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCaa                                                                    >  1:4539457/1‑149 (MQ=255)
                                                                                 atgaCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCaa                                                                    <  2:3195541/146‑1 (MQ=255)
                                                                                  ttaCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAAc                                                                   <  2:3911886/149‑1 (MQ=255)
                                                                                  ttaCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAAc                                                                   >  1:3559100/1‑149 (MQ=255)
                                                                                  ttaCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAAc                                                                   >  1:3153308/1‑149 (MQ=255)
                                                                                   taCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACg                                                                  >  1:989334/1‑149 (MQ=255)
                                                                                   taCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCGTCCATTGCGTCATAATCGCGAAGGACGGCCGCACTTACAGCAACg                                                                  >  2:3142801/1‑149 (MQ=255)
                                                                                    aCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGc                                                                 >  1:1000050/1‑149 (MQ=255)
                                                                                      cATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCt                                                               <  1:3623767/149‑1 (MQ=255)
                                                                                        tCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTaa                                                             >  2:643155/1‑149 (MQ=255)
                                                                                          ggTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAAc                                                           <  1:913517/149‑1 (MQ=255)
                                                                                          ggTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAAc                                                           <  1:2116902/149‑1 (MQ=255)
                                                                                              gttTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAAt                                                       <  2:2896950/149‑1 (MQ=255)
                                                                                               ttttCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATc                                                      <  1:1669761/149‑1 (MQ=255)
                                                                                               ttttCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATc                                                      <  1:3024738/149‑1 (MQ=255)
                                                                                                    aGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTTATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAg                                                 >  2:4351830/1‑149 (MQ=255)
                                                                                                     gAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGt                                                >  1:3435144/1‑149 (MQ=255)
                                                                                                          aTGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTtgctg                                           >  2:1756944/1‑149 (MQ=255)
                                                                                                           tGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTtgctgc                                          >  1:3168812/1‑149 (MQ=255)
                                                                                                             aaCATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGt                                        >  2:4287858/1‑149 (MQ=255)
                                                                                                                aTTGCTGCTTAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGAc                                     <  1:1081/149‑1 (MQ=255)
                                                                                                                 ttGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGAcc                                    <  1:3496109/149‑1 (MQ=255)
                                                                                                                     tgctgAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCa                                <  2:3674545/149‑1 (MQ=255)
                                                                                                                          aaGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCATTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAaca                           <  2:940650/149‑1 (MQ=255)
                                                                                                                           aGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAacac                          >  2:1296688/1‑149 (MQ=255)
                                                                                                                            gCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACt                         >  2:4640209/1‑149 (MQ=255)
                                                                                                                              aaCAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGt                       <  2:541305/149‑1 (MQ=255)
                                                                                                                               aCAGTAATAATGCCCAGTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATAGACAGTTGCTGCGTGACCGCCAGAACACTGtt                      <  2:33917/149‑1 (MQ=255)
                                                                                                                                cAGTAATAATGCCCATTGTTATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTa                     <  2:1616190/149‑1 (MQ=255)
                                                                                                                                    aataatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgc                 >  2:2380962/1‑149 (MQ=255)
                                                                                                                                    aataatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgc                 >  2:724582/1‑149 (MQ=255)
                                                                                                                                      taatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgctg               >  1:4080225/1‑149 (MQ=255)
                                                                                                                                       aatGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTACTCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgctgc              <  1:4330032/149‑1 (MQ=255)
                                                                                                                                           cccATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGCAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGc          <  2:4543215/149‑1 (MQ=255)
                                                                                                                                             cATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCAt        >  2:3761493/1‑149 (MQ=255)
                                                                                                                                             cATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCAt        >  1:809565/1‑149 (MQ=255)
                                                                                                                                               ttGTTATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGACGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTg      <  1:496812/149‑1 (MQ=255)
                                                                                                                                               ttGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTg      <  1:2704339/149‑1 (MQ=255)
                                                                                                                                                tgtgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGt     <  2:2479342/149‑1 (MQ=255)
                                                                                                                                                 gtgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTc    >  1:4595849/1‑149 (MQ=255)
                                                                                                                                                  tgATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCa   >  2:4247294/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCAT  >  CP009273/3932524‑3932819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑