Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,966,854 C→A 100% R325S (CGT→AGT)  rffG → dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,966,8540CA99.3% 967.2 / ‑2.1 281R325S (CGT→AGT) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (138/141);  total (138/143)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 281 total aligned reads displayed.

ACAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGG  >  CP009273/3966708‑3966999
                                                                                                                                                  |                                                                                                                                                 
aCAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGt                                                                                                                                                 >  2:1450421/1‑149 (MQ=255)
   aGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCCTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTGTGAAAGTGGAATGAGTaaa                                                                                                                                              >  2:3679077/1‑149 (MQ=255)
   aGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaaa                                                                                                                                              >  1:2515503/1‑149 (MQ=255)
   aGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaaa                                                                                                                                              <  2:3730903/149‑1 (MQ=255)
        cACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGt                                                                                                                                         <  2:4191185/149‑1 (MQ=255)
        cACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGt                                                                                                                                         <  2:174560/149‑1 (MQ=255)
        cACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGt                                                                                                                                         <  2:648179/149‑1 (MQ=255)
         aCGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGtt                                                                                                                                        <  1:337768/149‑1 (MQ=255)
         aCGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGtt                                                                                                                                        <  2:2639932/149‑1 (MQ=255)
           ggCGTGGCGCACTATCGTGACCTGATCTCCTTTGTCGCTGACCGTCCGGGGCATGTTCTGCGTTATGCCATTGTTGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCa                                                                                                                                      <  1:3951604/149‑1 (MQ=255)
           ggCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCa                                                                                                                                      <  1:1217402/149‑1 (MQ=255)
            gCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAg                                                                                                                                     <  1:2944610/149‑1 (MQ=255)
             cGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGt                                                                                                                                    <  1:3434845/149‑1 (MQ=255)
              gTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTg                                                                                                                                   <  2:3230890/149‑1 (MQ=255)
                ggCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGt                                                                                                                                 <  1:3600048/149‑1 (MQ=255)
                ggCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGt                                                                                                                                 >  2:4071134/1‑149 (MQ=255)
                     aCTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTg                                                                                                                            <  1:643359/149‑1 (MQ=255)
                      cTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTgg                                                                                                                           <  2:275084/149‑1 (MQ=255)
                      cTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTgg                                                                                                                           >  2:3906124/1‑149 (MQ=255)
                       tATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGc                                                                                                                          >  1:2351723/1‑149 (MQ=255)
                           gTGACCTGATCACCTTTGTCGCTGACCGTCCGGGTCATGATCGGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAATACGGTTCAGTGGTATCTGGCTAAt                                                                                                                      <  2:211322/149‑1 (MQ=255)
                           gTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAAt                                                                                                                      >  2:3143679/1‑149 (MQ=255)
                             gACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGa                                                                                                                    >  1:422471/1‑149 (MQ=255)
                             gACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGa                                                                                                                    >  2:460792/1‑149 (MQ=255)
                               ccTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGaaa                                                                                                                  >  2:1371394/1‑149 (MQ=255)
                               ccTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGaaa                                                                                                                  >  2:1323637/1‑149 (MQ=255)
                                 tGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc                                                                                                                <  2:26993/149‑1 (MQ=255)
                                  gATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCt                                                                                                               >  2:3455628/1‑149 (MQ=255)
                                   aTCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtg                                                                                                              <  2:4175419/149‑1 (MQ=255)
                                     cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggt                                                                                                            <  2:4406924/149‑1 (MQ=255)
                                     cACCTTGGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggt                                                                                                            <  2:1455798/149‑1 (MQ=255)
                                        cTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGa                                                                                                         <  1:140735/149‑1 (MQ=255)
                                         tttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTCTCTGGCTAATGAAAGCTGGTGGaa                                                                                                        >  2:3196447/1‑149 (MQ=255)
                                         tttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGaa                                                                                                        >  2:1273209/1‑149 (MQ=255)
                                         tttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGaa                                                                                                        >  2:1798296/1‑149 (MQ=255)
                                               gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGt                                                                                                  >  1:1007826/1‑149 (MQ=255)
                                                 tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGc                                                                                                >  2:3648416/1‑149 (MQ=255)
                                                       tCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACg                                                                                          >  2:3538589/1‑149 (MQ=255)
                                                       tCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACg                                                                                          >  2:3538437/1‑149 (MQ=255)
                                                        ccGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACgg                                                                                         >  2:1957585/1‑149 (MQ=255)
                                                        ccGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACgg                                                                                         <  1:1423453/149‑1 (MQ=255)
                                                           gggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAg                                                                                      <  2:1167470/149‑1 (MQ=255)
                                                            ggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGc                                                                                     <  1:3929482/149‑1 (MQ=255)
                                                              cATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTa                                                                                   >  2:2536703/1‑149 (MQ=255)
                                                               aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACGTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTAt                                                                                  <  1:1285151/149‑1 (MQ=255)
                                                                tGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATc                                                                                 <  1:1967371/149‑1 (MQ=255)
                                                                 gATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTCTCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCa                                                                                >  2:677998/1‑149 (MQ=255)
                                                                   tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAgg                                                                              <  1:1938484/149‑1 (MQ=255)
                                                                      gCGTTCTGCCTTTGATGCGTCGAAAAGTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGATAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCg                                                                           <  1:2702487/149‑1 (MQ=255)
                                                                               cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAg                                                                  >  1:4171702/1‑149 (MQ=255)
                                                                               cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAg                                                                  >  1:2190723/1‑149 (MQ=255)
                                                                               cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAg                                                                  >  1:2387627/1‑149 (MQ=255)
                                                                               cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAg                                                                  >  1:3796344/1‑149 (MQ=255)
                                                                                 ttGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGt                                                                >  2:1759728/1‑149 (MQ=255)
                                                                                    aTGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTg                                                             <  1:2536703/149‑1 (MQ=255)
                                                                                      gCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGaa                                                           <  2:4740771/149‑1 (MQ=255)
                                                                                         tCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAgg                                                        <  2:2307139/149‑1 (MQ=255)
                                                                                           gAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTCTCAGGGCGAGCGTTTAGGACTGAAAGGCt                                                      >  1:4686235/1‑149 (MQ=255)
                                                                                             aaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTaa                                                    >  2:2331574/1‑149 (MQ=255)
                                                                                                ttGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAAttt                                                 <  1:3183937/149‑1 (MQ=255)
                                                                                                   cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCa                                              >  2:2378730/1‑149 (MQ=255)
                                                                                                      gTGAACGTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCg                                           <  2:2430628/149‑1 (MQ=255)
                                                                                                        gAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGa                                         <  1:414079/149‑1 (MQ=255)
                                                                                                        gAACTGGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGa                                         <  2:1054655/149‑1 (MQ=255)
                                                                                                         aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAg                                        >  1:2622811/1‑149 (MQ=255)
                                                                                                         aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAg                                        <  1:1750983/149‑1 (MQ=255)
                                                                                                                 tggctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACAt                                <  1:373385/149‑1 (MQ=255)
                                                                                                                 tggctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACAt                                <  1:2084218/149‑1 (MQ=255)
                                                                                                                  ggctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATg                               <  2:2169056/149‑1 (MQ=255)
                                                                                                                  ggctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATg                               >  1:3555531/1‑149 (MQ=255)
                                                                                                                   gctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGa                              >  2:3398932/1‑149 (MQ=255)
                                                                                                                   gctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGa                              <  2:1920238/149‑1 (MQ=255)
                                                                                                                   gctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGa                              <  2:3904438/149‑1 (MQ=255)
                                                                                                                     tgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGaaa                            >  1:3873366/1‑149 (MQ=255)
                                                                                                                      gCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAg                           <  1:1344842/149‑1 (MQ=255)
                                                                                                                         gCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGta                        >  1:2924274/1‑149 (MQ=255)
                                                                                                                           aGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtatt                      <  2:2360611/149‑1 (MQ=255)
                                                                                                                            ggAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtatta                     >  2:2979458/1‑149 (MQ=255)
                                                                                                                            ggAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtatta                     >  1:4569077/1‑149 (MQ=255)
                                                                                                                             gAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtattat                    >  1:1053673/1‑149 (MQ=255)
                                                                                                                               aaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATcc                  >  2:3024172/1‑149 (MQ=255)
                                                                                                                               aaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATcc                  >  2:771521/1‑149 (MQ=255)
                                                                                                                               aaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATcc                  >  2:3787406/1‑149 (MQ=255)
                                                                                                                               aaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATcc                  >  1:4362696/1‑149 (MQ=255)
                                                                                                                                aCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCt                 >  2:2829282/1‑149 (MQ=255)
                                                                                                                                 ccTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTg                <  2:4491281/149‑1 (MQ=255)
                                                                                                                                  cTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTgg               >  2:3828260/1‑149 (MQ=255)
                                                                                                                                  cTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTgg               <  2:4255202/149‑1 (MQ=255)
                                                                                                                                  cTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTgg               >  2:4398999/1‑149 (MQ=255)
                                                                                                                                  cTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTgg               >  1:2695113/1‑149 (MQ=255)
                                                                                                                                   tttGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTggc              <  1:896115/149‑1 (MQ=255)
                                                                                                                                       aaaGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTggcgggc          <  1:2115771/149‑1 (MQ=255)
                                                                                                                                       aaaGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTggcgggc          <  1:2054483/149‑1 (MQ=255)
                                                                                                                                         aGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGgcgggcgg        >  2:579294/1‑149 (MQ=255)
                                                                                                                                          gTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGt       >  1:3679145/1‑149 (MQ=255)
                                                                                                                                          gTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGt       <  1:3354754/149‑1 (MQ=255)
                                                                                                                                             gAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTcc    >  1:3025350/1‑149 (MQ=255)
                                                                                                                                             gAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTcc    >  1:1689080/1‑149 (MQ=255)
                                                                                                                                             gAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGGTcc    >  1:4064002/1‑149 (MQ=255)
                                                                                                                                               aTGAGTAAAACGGTTCAGTGGTATCTGGCTAATGTAAGCTGGTGGAAGCAGGTGCAGGTCGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCTGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCTGTTCCgg  <  2:185363/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ACAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGG  >  CP009273/3966708‑3966999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑