Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,996,346 T→A 86.7% I70F (ATC→TTC)  yigG ← PRK11371 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,996,3460TA86.7% 912.5 / 111.1 316I70F (ATC→TTC) yigGPRK11371 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (23/19);  new base A (131/142);  total (155/161)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 330 total aligned reads displayed.

GCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATGAA  >  CP009273/3996198‑3996494
                                                                                                                                                    |                                                                                                                                                    
gCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAt                                                                                                                                                      >  1:4757642/1‑149 (MQ=255)
gCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAt                                                                                                                                                      <  1:3460011/149‑1 (MQ=255)
gCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAt                                                                                                                                                      <  1:1070752/149‑1 (MQ=255)
      aTTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAAcc                                                                                                                                                <  1:508892/149‑1 (MQ=255)
      aTTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAAcc                                                                                                                                                <  1:2901019/149‑1 (MQ=255)
       ttAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCa                                                                                                                                               >  1:2279950/1‑149 (MQ=255)
       ttAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCa                                                                                                                                               >  1:2400279/1‑149 (MQ=255)
        tAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCAc                                                                                                                                              <  2:3928636/149‑1 (MQ=255)
         aGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACt                                                                                                                                             >  2:1369769/1‑149 (MQ=255)
          gCATACTTAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTa                                                                                                                                            <  1:3093508/149‑1 (MQ=255)
          gCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCTAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTa                                                                                                                                            <  2:937691/149‑1 (MQ=255)
             tACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCa                                                                                                                                         >  1:2707264/1‑149 (MQ=255)
             tACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCa                                                                                                                                         <  1:4275380/149‑1 (MQ=255)
              aCTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCAc                                                                                                                                        <  1:2076173/149‑1 (MQ=255)
               cTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACg                                                                                                                                       >  2:670335/1‑149 (MQ=255)
                tGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACgg                                                                                                                                      >  1:4067581/1‑149 (MQ=255)
                  aaaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAg                                                                                                                                    <  2:309720/149‑1 (MQ=255)
                  aaaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAg                                                                                                                                    >  1:2962254/1‑149 (MQ=255)
                    aGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTc                                                                                                                                  <  2:3360179/149‑1 (MQ=255)
                       ggAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCt                                                                                                                               <  1:2666352/149‑1 (MQ=255)
                         aaaaaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTgg                                                                                                                             <  2:1553311/149‑1 (MQ=255)
                         aaaaaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTgg                                                                                                                             >  1:1210601/1‑149 (MQ=255)
                           aaaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTACTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCa                                                                                                                           <  2:3200992/149‑1 (MQ=255)
                            aaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCaa                                                                                                                          >  1:1408085/1‑149 (MQ=255)
                            aaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCaa                                                                                                                          >  1:3314172/1‑149 (MQ=255)
                              tAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAtt                                                                                                                        <  2:190707/149‑1 (MQ=255)
                                 cAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCAt                                                                                                                     >  1:4391846/1‑149 (MQ=255)
                                 cAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCAt                                                                                                                     <  1:2407725/149‑1 (MQ=255)
                                  aaaTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATg                                                                                                                    >  1:1323201/1‑149 (MQ=255)
                                   aaTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCACTTCATGt                                                                                                                   >  1:1232439/1‑149 (MQ=255)
                                      cAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtga                                                                                                                <  2:3972968/149‑1 (MQ=255)
                                          aaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTAAAGTTGATGAc                                                                                                            >  1:2222350/1‑149 (MQ=255)
                                             atcatcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACgag                                                                                                         <  1:3052470/149‑1 (MQ=255)
                                              tcctcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCACAAACATAAGTTTTTATATAGAAGAAACCACTATCCCGGAGTCGCTGGCAATTCATGTTGATGACgaga                                                                                                        <  2:2936609/146‑1 (MQ=255)
                                                 tcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATaa                                                                                                     <  2:4280098/149‑1 (MQ=255)
                                                 tcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATaa                                                                                                     >  2:430428/1‑149 (MQ=255)
                                                 tcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATaa                                                                                                     >  2:859926/1‑149 (MQ=255)
                                                      ccATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAg                                                                                                >  1:4175619/1‑149 (MQ=255)
                                                        aTTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTa                                                                                              <  1:848805/149‑1 (MQ=255)
                                                        aTTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTa                                                                                              <  1:2569821/149‑1 (MQ=255)
                                                         ttGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTAc                                                                                             >  2:3788351/1‑149 (MQ=255)
                                                           gCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATTTTGATGACGAGATAATGGAGTACGa                                                                                           >  1:1091714/1‑149 (MQ=255)
                                                           gCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGa                                                                                           <  2:4431132/149‑1 (MQ=255)
                                                                aaCAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTa                                                                                      >  1:3942152/1‑149 (MQ=255)
                                                                aaCAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTa                                                                                      <  1:4344330/149‑1 (MQ=255)
                                                                 aCAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTag                                                                                     >  2:4716576/1‑149 (MQ=255)
                                                                     gCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGAc                                                                                 >  1:4643112/1‑149 (MQ=255)
                                                                      cAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGAca                                                                                >  2:3560597/1‑148 (MQ=255)
                                                                       aaaaTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACta                                                                               <  1:586293/149‑1 (MQ=255)
                                                                         aaTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACtata                                                                             >  2:2525433/1‑149 (MQ=255)
                                                                         aaTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTTCGATGGTAGAGACtata                                                                             >  1:2989418/1‑149 (MQ=255)
                                                                           tCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAAc                                                                           >  1:1747376/1‑149 (MQ=255)
                                                                           tCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAAc                                                                           >  1:3029528/1‑149 (MQ=255)
                                                                             ttCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACaa                                                                         >  2:2196743/1‑149 (MQ=255)
                                                                             ttCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACaa                                                                         <  2:1323201/149‑1 (MQ=255)
                                                                                ggCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGaa                                                                      <  2:4045732/149‑1 (MQ=255)
                                                                                 gCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGaaa                                                                     <  1:276137/149‑1 (MQ=255)
                                                                                    aTCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGcc                                                                  >  2:2345882/1‑149 (MQ=255)
                                                                                       aTTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATTGAGTACGATGGTAGAGACTATAAAAAGAAAGCCTGc                                                               >  2:1673087/1‑149 (MQ=255)
                                                                                        ttGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCt                                                              <  1:3754656/149‑1 (MQ=255)
                                                                                        ttGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTAATGTTGATGGCGAGATAATGGAGTACGATGGTAGAGACTATAACAAAAAAGCCGGCt                                                              >  1:3764205/1‑149 (MQ=255)
                                                                                        ttGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAATAATGTTGATGACGAGATAATGGAGTACGATGGTAGATACAATAAAAAAAAAACCAGCt                                                              >  1:4426085/1‑149 (MQ=255)
                                                                                          gtgATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTtc                                                            <  1:1850893/149‑1 (MQ=255)
                                                                                          gtgATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTtc                                                            <  2:2897968/149‑1 (MQ=255)
                                                                                           tgatgaTAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTtct                                                           <  2:3314172/149‑1 (MQ=255)
                                                                                            gatgatAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTtctc                                                          <  2:1352668/149‑1 (MQ=255)
                                                                                                 tAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCg                                                     <  2:2618602/149‑1 (MQ=255)
                                                                                                     gatgatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaa                                                 <  1:3431721/149‑1 (MQ=255)
                                                                                                       tgatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaa                                               >  1:1147834/1‑149 (MQ=255)
                                                                                                         atatatACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaaaa                                             <  1:2102327/149‑1 (MQ=255)
                                                                                                         atatatACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaaaa                                             <  2:3445029/149‑1 (MQ=255)
                                                                                                          tatataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACAATAACAAGAAAGCCTGCTTCTCCATAGTTaaaaaat                                            >  2:2978844/1‑148 (MQ=255)
                                                                                                            tataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGat                                          <  1:1679825/149‑1 (MQ=255)
                                                                                                                cTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGataata                                      <  2:1013688/149‑1 (MQ=255)
                                                                                                                 tGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGAtaataa                                     <  2:515443/149‑1 (MQ=255)
                                                                                                                 tGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGAtaataa                                     >  2:4783560/1‑149 (MQ=255)
                                                                                                                     aaTGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGaaa                                 <  1:533166/149‑1 (MQ=255)
                                                                                                                       tGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAgg                               <  2:3589370/149‑1 (MQ=255)
                                                                                                                        ttACCAAAAACATAAGTTTTTATATAGAAGAAACAACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATTGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGc                              <  2:409447/148‑1 (MQ=255)
                                                                                                                        gTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGc                              <  2:731929/149‑1 (MQ=255)
                                                                                                                         tACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTAATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAACCTGCATCTCCAAAGTTAAAAAAGATAAAAAGAAATGcc                             >  1:4049361/1‑148 (MQ=255)
                                                                                                                          aacAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCaa                            <  2:3475073/147‑1 (MQ=255)
                                                                                                                          aCCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCaa                            >  2:4582464/1‑149 (MQ=255)
                                                                                                                          aCCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCaa                            <  1:938830/149‑1 (MQ=255)
                                                                                                                                  cATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATTTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCAGCTTCTCCATCGTTAAAAAAGATAATAAGAAATGCaaaaaataaa                    >  2:46778/1‑144 (MQ=255)
                                                                                                                                  cATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTAATGTTGATGACGAGATAATTGAGTAAGATGGTATAGACTATAAAAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAAGCAAAAATGaaa                    >  1:1433976/1‑149 (MQ=255)
                                                                                                                                  cATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAATCATGTTGATTACGAGATAATGGAGTACGCTGGTAGAGACTATAACAATAAAGACTGCATCTCCAACGTTAAAAAATAAAAAAAGAAAGGCaaaaaataaa                    >  1:3252280/1‑144 (MQ=255)
                                                                                                                                    tAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAAtt                  <  2:531799/149‑1 (MQ=255)
                                                                                                                                       gTTTTTATATAGAATAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATttat               <  2:762924/149‑1 (MQ=255)
                                                                                                                                       gTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATttat               <  2:3791559/149‑1 (MQ=255)
                                                                                                                                       gTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATttat               >  2:4663548/1‑149 (MQ=255)
                                                                                                                                        tttttATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATttatt              <  1:4684904/149‑1 (MQ=255)
                                                                                                                                         ttttATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATttatta             <  2:1230291/149‑1 (MQ=255)
                                                                                                                                          tttATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTaa            <  1:1271078/149‑1 (MQ=255)
                                                                                                                                            tatataGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTaaaa          <  1:199488/149‑1 (MQ=255)
                                                                                                                                             atataGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaat         <  2:888205/149‑1 (MQ=255)
                                                                                                                                                taGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataaa      >  2:1955202/1‑149 (MQ=255)
                                                                                                                                                 agaagaAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataaat     <  2:2707849/149‑1 (MQ=255)
                                                                                                                                                 agaagaAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataaat     <  2:2789996/149‑1 (MQ=255)
                                                                                                                                                 agaagaAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataaat     <  1:2329448/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATGAA  >  CP009273/3996198‑3996494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑