BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	335,247	C→A	87.2%	N404K (AAC→AAA)	yahG →	DUF1116 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	335,247	0	C	A	87.2%	636.3 / 103.1	281	N404K (AAC→AAA)	yahG	DUF1116 family protein
Reads supporting (aligned to +/- strand): ref base C (18/18); new base A (130/115); total (148/133)
Fisher's exact test for biased strand distribution p-value = 8.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.67e-01

Only 100 of 281 total aligned reads displayed.

GACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGT  >  CP009273/335101‑335390

 aCAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTc                                                                                                                                              >  2:3091875/1‑149 (MQ=255)

 aCAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATAACCCTCGGTGAAAACCCCAAAGTc                                                                                                                                              <  2:34093/149‑1 (MQ=255)

   aGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGTGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCAc                                                                                                                                            >  2:1326356/1‑149 (MQ=255)

    gcgcCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCAcc                                                                                                                                           >  1:2963047/1‑149 (MQ=255)

       ccATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCAtt                                                                                                                                        >  2:204561/1‑149 (MQ=255)

        cATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTc                                                                                                                                       <  1:227991/149‑1 (MQ=255)

         aTCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATAACCCTCGGTGAAAACCCCAAAGTCACCATTcc                                                                                                                                      <  1:987662/149‑1 (MQ=255)

         aTCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATAACCCTCGGTGAAAACCCCAAAGTCACCATTcc                                                                                                                                      <  1:988001/149‑1 (MQ=255)

            aCCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCgct                                                                                                                                   >  1:4442443/1‑149 (MQ=255)

            aCCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCgct                                                                                                                                   <  1:4664215/149‑1 (MQ=255)

            aCCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCgct                                                                                                                                   <  1:667598/149‑1 (MQ=255)

            aCCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCgct                                                                                                                                   >  1:3108753/1‑149 (MQ=255)

                aaaCCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTc                                                                                                                               <  1:3540794/149‑1 (MQ=255)

                   ccTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGt                                                                                                                            <  2:96254/149‑1 (MQ=255)

                   ccTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGt                                                                                                                            <  1:4194018/149‑1 (MQ=255)

                   ccTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGt                                                                                                                            <  2:589100/149‑1 (MQ=255)

                    cTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGtt                                                                                                                           <  2:788247/149‑1 (MQ=255)

                    cTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGtt                                                                                                                           <  1:2645127/149‑1 (MQ=255)

                        ggTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTAt                                                                                                                       <  1:2353388/149‑1 (MQ=255)

                        ggTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTAt                                                                                                                       >  1:2234266/1‑149 (MQ=255)

                         gTATTGGCGGGTTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATg                                                                                                                      <  2:4295616/149‑1 (MQ=255)

                             tGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCg                                                                                                                  <  1:1249760/149‑1 (MQ=255)

                                     ttGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCg                                                                                                          >  2:2299761/1‑149 (MQ=255)

                                       gCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGc                                                                                                        >  1:1214109/1‑149 (MQ=255)

                                         tATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCaa                                                                                                      <  1:3677520/149‑1 (MQ=255)

                                          aTGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAAt                                                                                                     <  2:3115270/149‑1 (MQ=255)

                                          aTGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAAt                                                                                                     >  2:1650249/1‑149 (MQ=255)

                                           tGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATc                                                                                                    <  1:1706658/149‑1 (MQ=255)

                                             gCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGa                                                                                                  <  1:1889296/149‑1 (MQ=255)

                                                aCCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACAt                                                                                               >  2:352419/1‑149 (MQ=255)

                                                aCCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACAt                                                                                               >  2:2744598/1‑149 (MQ=255)

                                                aCCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACAt                                                                                               <  1:1131928/149‑1 (MQ=255)

                                                aCCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACAt                                                                                               >  2:2184210/1‑149 (MQ=255)

                                                   gcgcCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCAc                                                                                            >  2:4075402/1‑149 (MQ=255)

                                                    cgcCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCAcc                                                                                           <  1:3248254/149‑1 (MQ=255)

                                                      cccGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCcg                                                                                         >  2:350256/1‑149 (MQ=255)

                                                         gCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGt                                                                                      >  2:1883020/1‑149 (MQ=255)

                                                           tATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTgg                                                                                    <  1:2746589/149‑1 (MQ=255)

                                                           tATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTgg                                                                                    >  2:3824080/1‑149 (MQ=255)

                                                           tATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTgg                                                                                    >  1:3277660/1‑149 (MQ=255)

                                                            aTCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTggg                                                                                   <  2:1300141/149‑1 (MQ=255)

                                                             tcgtcgCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGt                                                                                  <  2:2403565/149‑1 (MQ=255)

                                                             tcgtcgCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGt                                                                                  <  1:3395477/149‑1 (MQ=255)

                                                                 cgcgCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTagca                                                                              >  1:3985839/1‑149 (MQ=255)

                                                                       ggtggGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCa                                                                        >  2:1503793/1‑149 (MQ=255)

                                                                       ggtggGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCa                                                                        >  1:1638914/1‑149 (MQ=255)

                                                                         tggGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCAtt                                                                      <  1:2939984/149‑1 (MQ=255)

                                                                             cggcACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGc                                                                  <  2:124104/149‑1 (MQ=255)

                                                                              ggcACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGcc                                                                 >  2:387190/1‑149 (MQ=255)

                                                                              ggcACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGcc                                                                 >  1:4722165/1‑149 (MQ=255)

                                                                              ggcACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGcc                                                                 <  1:731784/149‑1 (MQ=255)

                                                                              ggcACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGcc                                                                 >  1:4025564/1‑149 (MQ=255)

                                                                               gcACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCCTTCTGCCg                                                                >  1:2548598/1‑149 (MQ=255)

                                                                                   ggTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGa                                                            <  2:4736170/149‑1 (MQ=255)

                                                                                    gTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGAt                                                           >  1:114634/1‑149 (MQ=255)

                                                                                         agaagCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAaca                                                      >  2:2888243/1‑149 (MQ=255)

                                                                                          gaagCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAacac                                                     <  2:4467662/149‑1 (MQ=255)

                                                                                          gaagCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAacac                                                     <  1:3894743/149‑1 (MQ=255)

                                                                                           aagCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACAcc                                                    <  1:4472516/149‑1 (MQ=255)

                                                                                           aagCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACAcc                                                    <  1:512777/149‑1 (MQ=255)

                                                                                            agCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCg                                                   >  1:1966170/1‑149 (MQ=255)

                                                                                              cTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGcc                                                 <  1:592546/149‑1 (MQ=255)

                                                                                               tATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCa                                                >  1:290003/1‑149 (MQ=255)

                                                                                               tATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCa                                                >  2:4168999/1‑149 (MQ=255)

                                                                                                aTTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCAt                                               >  1:1865501/1‑149 (MQ=255)

                                                                                                    aTTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGcc                                           <  1:3538791/149‑1 (MQ=255)

                                                                                                    aTTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGcc                                           >  1:1048548/1‑149 (MQ=255)

                                                                                                      ttCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCATCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCa                                         <  2:426515/149‑1 (MQ=255)

                                                                                                          cccGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATaaa                                     >  2:4575373/1‑149 (MQ=255)

                                                                                                           ccGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAg                                    >  2:497587/1‑149 (MQ=255)

                                                                                                             gTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGAt                                  <  2:194943/149‑1 (MQ=255)

                                                                                                              tCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATg                                 >  1:4628145/1‑149 (MQ=255)

                                                                                                              tCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATg                                 <  2:458317/149‑1 (MQ=255)

                                                                                                              tCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATg                                 >  2:2034050/1‑149 (MQ=255)

                                                                                                                aGATGCGCGCAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCg                               <  2:3816456/149‑1 (MQ=255)

                                                                                                                aGATGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCg                               >  2:3376415/1‑149 (MQ=255)

                                                                                                                  aTGCGCGAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCggg                             <  1:4079208/149‑1 (MQ=255)

                                                                                                                      gcgAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTc                         >  2:2675026/1‑149 (MQ=255)

                                                                                                                      gcgAAATCACCCTCGGTGAAAACCCCAAAGTAACCATACCGCTGCTCGGATTTATGGGCGTGCCGTCTGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCCACACCGCCATAGCACATAAAGATGCGGGCGTc                         >  2:283461/1‑149 (MQ=255)

                                                                                                                       cgAAATCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCg                        <  2:2491146/149‑1 (MQ=255)

                                                                                                                         aaaTCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGc                      >  1:361279/1‑149 (MQ=255)

                                                                                                                         aaaTCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGc                      <  1:126602/149‑1 (MQ=255)

                                                                                                                          aaTCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCa                     <  2:1748510/149‑1 (MQ=255)

                                                                                                                          aaTCACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCa                     >  2:2009227/1‑149 (MQ=255)

                                                                                                                             cACCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGa                  <  1:4303989/149‑1 (MQ=255)

                                                                                                                              aCCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGAt                 <  2:3651443/149‑1 (MQ=255)

                                                                                                                              aCCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGAt                 >  1:873819/1‑149 (MQ=255)

                                                                                                                              aCCCTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGGGGGTAGCGGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCAGAATGTTGCGGGCGTCGGCATGAt                 >  1:1973349/1‑149 (MQ=255)

                                                                                                                                ccTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTg               <  1:2619761/149‑1 (MQ=255)

                                                                                                                                 cTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAAGACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTgg              <  2:3009237/149‑1 (MQ=255)

                                                                                                                                 cTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTgg              >  1:1400070/1‑149 (MQ=255)

                                                                                                                                 cTCGGTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGGCGGCATGATTgg              >  1:4279380/1‑149 (MQ=255)

                                                                                                                                    ggTGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGgcgc           >  1:4532889/1‑149 (MQ=255)

                                                                                                                                      tGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCgg         <  2:2563467/149‑1 (MQ=255)

                                                                                                                                      tGAAAACCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCgg         <  2:1089153/149‑1 (MQ=255)

                                                                                                                                          aaCCCCAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCAt     >  2:884893/1‑149 (MQ=255)

                                                                                                                                            ccccAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATtg   >  2:4533820/1‑149 (MQ=255)

                                                                                                                                            ccccAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATtg   >  1:276219/1‑149 (MQ=255)

                                                                                                                                             cccAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATtgt  >  1:2058507/1‑149 (MQ=255)

                                                                                                                                             cccAAAGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATtgt  >  1:1469389/1‑149 (MQ=255)

GACAGCGCCATCACCGAAACCTACGGTATTGGCGGATTTGCTATGGCGACCGCGCCCGCTATCGTCGCGCTGGTGGGCGGCACGGTGGAAGAAGCTATTGATTTCTCCCGTCAGATGCGCGAAATCACCCTCGGTGAAAACCCCAACGTCACCATTCCGCTGCTCGGTTTTATGGGCGTGCCGTCGGCAATCGACATCACCCGCGTGGGTAGCAGCGGCATTCTGCCGGTGATCAACACCGCCATCGCCCATAAAGATGCGGGCGTCGGCATGATTGGCGCGGGCATTGT  >  CP009273/335101‑335390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`