Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,058,809 G→T 86.9% I106I (ATC→ATA)  yihO ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,058,8090GT86.9% 667.9 / 105.6 292I106I (ATC→ATA) yihOputative transporter
Reads supporting (aligned to +/- strand):  ref base G (24/15);  new base T (112/141);  total (136/156)
Fisher's exact test for biased strand distribution p-value = 5.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 292 total aligned reads displayed.

GCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC  >  CP009273/4058663‑4058956
                                                                                                                                                  |                                                                                                                                                   
gcgAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTAt                                                                                                                                                   >  2:526936/1‑149 (MQ=255)
   aGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATaaa                                                                                                                                                >  2:1298638/1‑149 (MQ=255)
   aGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATaaa                                                                                                                                                <  2:600182/149‑1 (MQ=255)
    gCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAAc                                                                                                                                               <  2:2899165/149‑1 (MQ=255)
    gCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAAc                                                                                                                                               <  2:3746783/149‑1 (MQ=255)
        cgcgcgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGa                                                                                                                                           <  1:898911/149‑1 (MQ=255)
            cgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGt                                                                                                                                       <  2:2181024/149‑1 (MQ=255)
            cgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGt                                                                                                                                       <  1:2084207/149‑1 (MQ=255)
                 aTTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCaa                                                                                                                                  <  2:740983/149‑1 (MQ=255)
                   tCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAAtt                                                                                                                                <  2:1621569/149‑1 (MQ=255)
                       gTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcg                                                                                                                            <  1:4027305/149‑1 (MQ=255)
                        tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcgc                                                                                                                           >  1:2287500/1‑149 (MQ=255)
                        tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcgc                                                                                                                           >  1:4683960/1‑149 (MQ=255)
                        tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAAACGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcgc                                                                                                                           >  1:4063161/1‑149 (MQ=255)
                         ttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcgcc                                                                                                                          <  2:931155/149‑1 (MQ=255)
                         ttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAAcgcc                                                                                                                          <  1:271638/149‑1 (MQ=255)
                           ttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcgccgc                                                                                                                        <  1:3048666/149‑1 (MQ=255)
                           ttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcgccgc                                                                                                                        <  1:4123956/149‑1 (MQ=255)
                              gTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCgg                                                                                                                     >  2:4665422/1‑149 (MQ=255)
                              gTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCgg                                                                                                                     <  2:3118216/149‑1 (MQ=255)
                                aaTTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATAATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGaa                                                                                                                   <  2:2738919/149‑1 (MQ=255)
                                aaTTGCCGGGATAATCGCACCATACGAGCAGTTCATCAGACTTTATGAAAGTCAAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGaa                                                                                                                   <  1:1621444/149‑1 (MQ=255)
                                    gCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGc                                                                                                               >  1:70259/1‑149 (MQ=255)
                                    gCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGc                                                                                                               <  2:4108512/149‑1 (MQ=255)
                                       gggATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGc                                                                                                            >  1:3066574/1‑149 (MQ=255)
                                       gggATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGc                                                                                                            >  2:4758259/1‑149 (MQ=255)
                                          atcatcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCata                                                                                                         <  2:4146865/149‑1 (MQ=255)
                                           tcatcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCatat                                                                                                        <  1:4536677/149‑1 (MQ=255)
                                            catcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCAtata                                                                                                       <  1:4342278/149‑1 (MQ=255)
                                             atcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataa                                                                                                      >  1:1422003/1‑149 (MQ=255)
                                             atcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataa                                                                                                      >  1:135384/1‑149 (MQ=255)
                                              tcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGTAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaa                                                                                                     >  2:1640519/1‑149 (MQ=255)
                                               cGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaa                                                                                                    <  1:747899/149‑1 (MQ=255)
                                               cGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaa                                                                                                    <  1:245551/149‑1 (MQ=255)
                                                 cACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaata                                                                                                  >  2:4216768/1‑149 (MQ=255)
                                                   ccATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaataaa                                                                                                >  1:1490648/1‑149 (MQ=255)
                                                     aTACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAgg                                                                                              <  2:209735/149‑1 (MQ=255)
                                                      tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAggg                                                                                             >  2:1445460/1‑149 (MQ=255)
                                                      tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAggg                                                                                             >  2:3799307/1‑149 (MQ=255)
                                                       aCGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGa                                                                                            <  2:2324041/149‑1 (MQ=255)
                                                       aCGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGa                                                                                            <  1:2897443/149‑1 (MQ=255)
                                                        cGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGAc                                                                                           <  2:3048490/149‑1 (MQ=255)
                                                          aGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGa                                                                                         <  1:3304053/149‑1 (MQ=255)
                                                               ttCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAttt                                                                                    <  2:3629413/149‑1 (MQ=255)
                                                                 catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGc                                                                                  >  1:3690433/1‑149 (MQ=255)
                                                                 catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGc                                                                                  <  2:3412912/149‑1 (MQ=255)
                                                                     aGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAACAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATGTGCCttt                                                                              <  2:680469/149‑1 (MQ=255)
                                                                     aGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCttt                                                                              <  2:1744564/149‑1 (MQ=255)
                                                                      gACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTa                                                                             >  1:1146039/1‑149 (MQ=255)
                                                                       aCTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAg                                                                            >  1:254359/1‑149 (MQ=255)
                                                                        cTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAgg                                                                           >  2:3365192/1‑149 (MQ=255)
                                                                         tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGc                                                                          <  1:2996379/149‑1 (MQ=255)
                                                                         tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGc                                                                          <  1:354667/149‑1 (MQ=255)
                                                                          gTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGcc                                                                         <  2:3144968/149‑1 (MQ=255)
                                                                             tGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCaa                                                                      >  2:1091945/1‑149 (MQ=255)
                                                                               aaaGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAata                                                                    >  1:1080678/1‑149 (MQ=255)
                                                                               aaaGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAata                                                                    <  1:2670910/149‑1 (MQ=255)
                                                                               aaaGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAata                                                                    >  2:137363/1‑149 (MQ=255)
                                                                                 aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtt                                                                  <  2:3254720/149‑1 (MQ=255)
                                                                                  gTCCAAACATCATACACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAttt                                                                 <  2:997003/149‑1 (MQ=255)
                                                                                    ccAAAAATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGAAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAttttt                                                               <  1:4211875/149‑1 (MQ=255)
                                                                                       aaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCcg                                                            >  2:1457235/1‑149 (MQ=255)
                                                                                        aCATCATAAACAGCGCGGTGGCAATCGTCGTGTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGGTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCcgc                                                           >  1:3327304/1‑149 (MQ=255)
                                                                                          atcatAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcga                                                         <  2:550134/149‑1 (MQ=255)
                                                                                           tcatAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgag                                                        <  1:1531906/149‑1 (MQ=255)
                                                                                              tAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcg                                                     <  2:2602655/149‑1 (MQ=255)
                                                                                                caCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcgag                                                   <  2:2803607/148‑1 (MQ=255)
                                                                                                aaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCCAACAAAAGGTGGTGGCTATCAACGGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATCTAAAATAAAGGGACGAAATTTGCCGTTAGGCCCAATATTTTTCCGcgagtcgag                                                   >  2:4621859/1‑149 (MQ=255)
                                                                                                aaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcgag                                                   >  2:4416606/1‑149 (MQ=255)
                                                                                                aaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcgag                                                   <  2:2008617/149‑1 (MQ=255)
                                                                                                aaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcgag                                                   <  1:4396180/149‑1 (MQ=255)
                                                                                                aaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcgag                                                   <  2:3324759/149‑1 (MQ=255)
                                                                                                aaCAGCGCGGTGGCAATCGTCGTTTTAAACGGCAAACAAAAGGTGGTGGCTAAAAACTTGAGCGTTTCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAAAAATTTTCCGcgagtcgag                                                   >  1:3483929/1‑149 (MQ=255)
                                                                                                         gTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATcc                                          >  2:2965005/1‑149 (MQ=255)
                                                                                                         gTGGCAATCGTCGTGTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATcc                                          >  1:2384316/1‑149 (MQ=255)
                                                                                                          tGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCg                                         >  2:1699995/1‑149 (MQ=255)
                                                                                                           ggCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCgg                                        >  2:2308815/1‑149 (MQ=255)
                                                                                                             cactcgtcgTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTg                                      <  1:3857302/146‑1 (MQ=255)
                                                                                                             cAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTg                                      <  1:588436/149‑1 (MQ=255)
                                                                                                             cAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTg                                      <  1:2761815/149‑1 (MQ=255)
                                                                                                             cAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTg                                      <  1:4755602/149‑1 (MQ=255)
                                                                                                             cAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTg                                      <  2:264249/149‑1 (MQ=255)
                                                                                                                   tcgtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCata                                >  1:496532/1‑149 (MQ=255)
                                                                                                                    cgtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCatat                               <  1:3715964/149‑1 (MQ=255)
                                                                                                                     tttttAACATGCAAACAAAAGGTTGTGGATATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCTCTGCATATAAAATAAAGTGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATc                              <  2:3587002/148‑1 (MQ=255)
                                                                                                                     gtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATc                              >  1:1007914/1‑149 (MQ=255)
                                                                                                                     gtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATc                              >  1:4582949/1‑149 (MQ=255)
                                                                                                                      ttttAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCg                             >  1:4634549/1‑149 (MQ=255)
                                                                                                                       tttAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCgg                            >  2:3856750/1‑149 (MQ=255)
                                                                                                                        ttAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGt                           >  1:2037923/1‑149 (MQ=255)
                                                                                                                            ccGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAc                       <  2:4302952/149‑1 (MQ=255)
                                                                                                                                caaacaaaAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCgg                   <  2:1923506/149‑1 (MQ=255)
                                                                                                                                 aaacaaaAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGt                  <  1:4416606/149‑1 (MQ=255)
                                                                                                                                  aacaaaAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTa                 >  1:316821/1‑149 (MQ=255)
                                                                                                                                   acaaaAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaa                >  2:3711047/1‑149 (MQ=255)
                                                                                                                                      acaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaa             <  2:4053436/147‑1 (MQ=255)
                                                                                                                                      aaaGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaa             <  1:4006280/149‑1 (MQ=255)
                                                                                                                                        aGGTTGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAAc           <  2:2349607/149‑1 (MQ=255)
                                                                                                                                        aGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAAc           >  1:3392187/1‑149 (MQ=255)
                                                                                                                                            ggtggCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCg       <  1:1930847/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC  >  CP009273/4058663‑4058956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑