Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,094,362 C→A 100% W181L (TGG→TTG)  cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,094,3620CA99.6% 978.8 / ‑4.2 283W181L (TGG→TTG) cpxAsensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (107/174);  total (108/175)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 296 total aligned reads displayed.

TGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGCCATCACGCACGGA  >  CP009273/4094216‑4094507
                                                                                                                                                  |                                                                                                                                                 
tGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCaacaac                                                                                                                                                 <  2:3192309/149‑1 (MQ=255)
 gACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAacaaca                                                                                                                                                <  1:387531/149‑1 (MQ=255)
 gACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAacaaca                                                                                                                                                >  2:4215416/1‑149 (MQ=255)
   ccATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCaacaacaac                                                                                                                                              >  2:2326969/1‑149 (MQ=255)
   ccATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCaacaacaac                                                                                                                                              >  2:3633361/1‑149 (MQ=255)
     aTCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAAcag                                                                                                                                            <  2:2033106/149‑1 (MQ=255)
     aTCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAAcag                                                                                                                                            >  2:3576025/1‑149 (MQ=255)
     aTCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAAcag                                                                                                                                            <  2:2804241/149‑1 (MQ=255)
       cTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAAcagca                                                                                                                                          <  1:4677937/149‑1 (MQ=255)
       cTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAAcagca                                                                                                                                          <  2:1872136/149‑1 (MQ=255)
        tGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAAcagcag                                                                                                                                         <  1:2062601/149‑1 (MQ=255)
        tGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAAcagcag                                                                                                                                         <  1:3301669/149‑1 (MQ=255)
        tGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAAcagcag                                                                                                                                         <  1:49274/149‑1 (MQ=255)
        tGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAAcagcag                                                                                                                                         <  1:1378123/149‑1 (MQ=255)
            tAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAAGTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAgcggc                                                                                                                                     <  1:169388/149‑1 (MQ=255)
              aaaCTGGCACCTGCGGCACGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCCGGCCAACAACAACAGCAGCGGCGt                                                                                                                                   >  1:3713662/1‑149 (MQ=255)
                aCTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTAc                                                                                                                                 <  1:2076831/149‑1 (MQ=255)
                aCTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTAc                                                                                                                                 >  2:3482758/1‑149 (MQ=255)
                 cTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACt                                                                                                                                <  2:4700/149‑1 (MQ=255)
                 cTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACt                                                                                                                                >  2:3855235/1‑149 (MQ=255)
                  tGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTg                                                                                                                               <  1:1475925/149‑1 (MQ=255)
                  tGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTg                                                                                                                               <  1:4515491/149‑1 (MQ=255)
                  tGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTg                                                                                                                               >  2:1111642/1‑149 (MQ=255)
                    gCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGAc                                                                                                                             <  2:2064159/149‑1 (MQ=255)
                    gCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGAc                                                                                                                             <  2:2768109/149‑1 (MQ=255)
                     cACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGAcc                                                                                                                            <  2:2738851/149‑1 (MQ=255)
                       ccTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCaa                                                                                                                          <  2:4357300/149‑1 (MQ=255)
                        cTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAAc                                                                                                                         <  1:4598885/149‑1 (MQ=255)
                          gCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACAt                                                                                                                       >  2:2409003/1‑149 (MQ=255)
                          gCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACAt                                                                                                                       <  2:2652085/149‑1 (MQ=255)
                             gCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGt                                                                                                                    >  2:4331735/1‑149 (MQ=255)
                                 ggAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACa                                                                                                                >  2:978122/1‑149 (MQ=255)
                                   aaTTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAAt                                                                                                              <  1:664839/149‑1 (MQ=255)
                                   aaTTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAAt                                                                                                              >  1:4776142/1‑149 (MQ=255)
                                   aaTTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAAt                                                                                                              <  1:2024714/149‑1 (MQ=255)
                                    aTTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCCGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATc                                                                                                             >  1:2729307/1‑149 (MQ=255)
                                    aTTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATc                                                                                                             >  1:1620374/1‑149 (MQ=255)
                                        cTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcagca                                                                                                         <  2:717939/149‑1 (MQ=255)
                                           tgGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTa                                                                                                      >  1:2550887/1‑149 (MQ=255)
                                            ggCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTaa                                                                                                     <  1:1723897/149‑1 (MQ=255)
                                            ggCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTaa                                                                                                     <  1:419972/149‑1 (MQ=255)
                                            ggCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTaa                                                                                                     <  1:1276250/149‑1 (MQ=255)
                                              ccccGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATa                                                                                                   >  2:3017987/1‑149 (MQ=255)
                                              ccccGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATa                                                                                                   <  1:962856/149‑1 (MQ=255)
                                               cccGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAg                                                                                                  <  1:1774795/149‑1 (MQ=255)
                                               cccGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAg                                                                                                  <  1:2295859/149‑1 (MQ=255)
                                               cccGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAg                                                                                                  <  1:4050748/149‑1 (MQ=255)
                                               cccGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAg                                                                                                  <  1:2498849/149‑1 (MQ=255)
                                                   cTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcggg                                                                                              >  1:1728154/1‑149 (MQ=255)
                                                        aGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTc                                                                                         >  2:249116/1‑149 (MQ=255)
                                                            ccGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAAc                                                                                     >  2:2688111/1‑149 (MQ=255)
                                                            ccGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAAc                                                                                     <  2:2580412/149‑1 (MQ=255)
                                                            ccGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAAc                                                                                     <  1:3502658/149‑1 (MQ=255)
                                                                       cGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAAt                                                                          <  2:1685678/149‑1 (MQ=255)
                                                                         tAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGa                                                                        >  1:3780098/1‑149 (MQ=255)
                                                                           aGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGaaa                                                                      <  2:1896504/149‑1 (MQ=255)
                                                                           aGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGaaa                                                                      <  1:3005661/149‑1 (MQ=255)
                                                                            gTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAAt                                                                     <  2:1801327/149‑1 (MQ=255)
                                                                            gTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAAt                                                                     >  1:2691192/1‑149 (MQ=255)
                                                                               tCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCgg                                                                  >  1:3233557/1‑149 (MQ=255)
                                                                                cccTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGa                                                                 <  1:2675388/149‑1 (MQ=255)
                                                                                 ccTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGAt                                                                <  1:4295404/149‑1 (MQ=255)
                                                                                   tGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTg                                                              >  2:4538927/1‑149 (MQ=255)
                                                                                       cAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGaa                                                          <  2:2218741/149‑1 (MQ=255)
                                                                                        aaCTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAAc                                                         <  2:1183825/149‑1 (MQ=255)
                                                                                        aaCTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAAc                                                         <  2:791344/149‑1 (MQ=255)
                                                                                         aCTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAAct                                                        >  1:2869692/1‑149 (MQ=255)
                                                                                         aCTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAAct                                                        >  1:3192088/1‑149 (MQ=255)
                                                                                          cTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAActg                                                       >  2:885860/1‑149 (MQ=255)
                                                                                            tCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAActgct                                                     >  1:3950166/1‑149 (MQ=255)
                                                                                            tCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAActgct                                                     >  1:275506/1‑149 (MQ=255)
                                                                                               tCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGc                                                  <  1:3061383/149‑1 (MQ=255)
                                                                                                  gCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCgg                                               >  2:3760412/1‑149 (MQ=255)
                                                                                                     gCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACg                                            >  2:639923/1‑149 (MQ=255)
                                                                                                       gTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGaa                                          >  2:4586483/1‑149 (MQ=255)
                                                                                                        tttttCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAAt                                         <  1:1679217/149‑1 (MQ=255)
                                                                                                        tttttCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAAt                                         <  1:1305534/149‑1 (MQ=255)
                                                                                                         ttttCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATc                                        <  2:3772205/149‑1 (MQ=255)
                                                                                                          tttCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCa                                       <  1:3449894/149‑1 (MQ=255)
                                                                                                          tttCAGCTTACGCGCCGGTTTTGCAAGACTCCAGGCCAACAACAACTGCAGCGGCGTACTGACCAACATGGTGTCATTCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCa                                       <  1:1566718/149‑1 (MQ=255)
                                                                                                           ttCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAg                                      <  2:4603665/149‑1 (MQ=255)
                                                                                                                cTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATaaa                                 <  1:2805187/149‑1 (MQ=255)
                                                                                                                  tACGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGt                               >  1:1262952/1‑149 (MQ=255)
                                                                                                                   aCGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGtt                              <  1:4641094/149‑1 (MQ=255)
                                                                                                                   aCGCGCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGtt                              <  2:1978333/149‑1 (MQ=255)
                                                                                                                    cgcgCCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTg                             <  1:23444/149‑1 (MQ=255)
                                                                                                                     gcgcCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTgg                            >  1:4754982/1‑149 (MQ=255)
                                                                                                                       gcCGGTTTTGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTa                          <  1:1078733/149‑1 (MQ=255)
                                                                                                                            ttttGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTAt                     >  2:1262174/1‑149 (MQ=255)
                                                                                                                               tgtcAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCtt                  <  1:2828289/146‑1 (MQ=255)
                                                                                                                               tGCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCtt                  <  1:898745/149‑1 (MQ=255)
                                                                                                                                gCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTc                 >  2:3233787/1‑149 (MQ=255)
                                                                                                                                gCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTc                 <  1:4356456/149‑1 (MQ=255)
                                                                                                                                gCCAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTc                 <  2:3642666/149‑1 (MQ=255)
                                                                                                                                  cAGACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGc               >  2:3714121/1‑149 (MQ=255)
                                                                                                                                    gACTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGCCa             >  1:1855183/1‑149 (MQ=255)
                                                                                                                                     aCTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGCCAt            <  2:2644786/149‑1 (MQ=255)
                                                                                                                                      cTCCAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGCCATc           >  1:1086143/1‑149 (MQ=255)
                                                                                                                                        ccAGGCCAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGCCATcac         <  1:1204018/149‑1 (MQ=255)
                                                                                                                                             ccAACAACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGCCATcacgcacg    >  1:754615/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTTAATGAAATCGGATTGAGAACTGCTGGCCGGACGAATCAGATAAAGTTGGTAATTATCTTCGCCATCACGCACGGA  >  CP009273/4094216‑4094507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑