Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,106,085 A→T 24.0% Y356N (TAC→AAC)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,106,0850AT24.0% 588.6 / 203.0 283Y356N (TAC→AAC) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base A (91/124);  new base T (36/32);  total (127/156)
Fisher's exact test for biased strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 283 total aligned reads displayed.

GCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACAC  >  CP009273/4105938‑4106232
                                                                                                                                                   |                                                                                                                                                   
gCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAc                                                                                                                                                    >  1:3841384/1‑149 (MQ=255)
   gACGGATACCAGAGTCGGCCTGCATCGCTTCCCGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCTTACGGGTCCCAGTACGgc                                                                                                                                                 <  2:545363/149‑1 (MQ=255)
   gACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGgc                                                                                                                                                 <  2:1639749/149‑1 (MQ=255)
      ggATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCa                                                                                                                                              >  1:1248099/1‑149 (MQ=255)
         tACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAccc                                                                                                                                           >  2:3816792/1‑149 (MQ=255)
         tACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAccc                                                                                                                                           <  1:1275955/149‑1 (MQ=255)
          aCCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCa                                                                                                                                          >  2:2538588/1‑149 (MQ=255)
            cAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGc                                                                                                                                        <  2:1261743/149‑1 (MQ=255)
            cAGAGTCGGCCTACATCGCTTCCAGCACGTCACGCGTCTTATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGc                                                                                                                                        <  1:1113995/149‑1 (MQ=255)
            cAGAGTCGGCCTACATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGc                                                                                                                                        <  1:3502147/149‑1 (MQ=255)
             agagTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGcc                                                                                                                                       <  1:4351649/149‑1 (MQ=255)
                gTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCgg                                                                                                                                    <  2:1900034/149‑1 (MQ=255)
                  cGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTa                                                                                                                                  <  1:1708438/149‑1 (MQ=255)
                     ccTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAAt                                                                                                                               >  2:1168729/1‑149 (MQ=255)
                         cATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCg                                                                                                                           <  2:3119820/149‑1 (MQ=255)
                            cGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGaa                                                                                                                        <  2:2344386/149‑1 (MQ=255)
                             gcgtCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAAc                                                                                                                       >  2:2748237/4‑149 (MQ=255)
                             gCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAAc                                                                                                                       <  1:3044741/149‑1 (MQ=255)
                             gCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAAc                                                                                                                       <  1:3647517/149‑1 (MQ=255)
                                     cACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATaca                                                                                                               <  2:3590706/149‑1 (MQ=255)
                                      aCGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATacac                                                                                                              <  1:2321878/149‑1 (MQ=255)
                                        gTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATacacac                                                                                                            <  2:3533038/149‑1 (MQ=255)
                                           aCGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAt                                                                                                         <  1:3785710/149‑1 (MQ=255)
                                             gcgTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTg                                                                                                       <  2:4255641/149‑1 (MQ=255)
                                               gTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGgt                                                                                                     >  1:3767265/1‑149 (MQ=255)
                                                tCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGgtg                                                                                                    <  2:2934531/149‑1 (MQ=255)
                                                tCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGgtg                                                                                                    >  2:4297789/1‑149 (MQ=255)
                                                  tGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGtt                                                                                                  >  1:1676628/1‑149 (MQ=255)
                                                   gATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGttt                                                                                                 <  2:63577/149‑1 (MQ=255)
                                                    aTAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGtttt                                                                                                >  1:4332502/1‑149 (MQ=255)
                                                      aaGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGc                                                                                              >  1:1979345/1‑149 (MQ=255)
                                                       aGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCa                                                                                             >  1:3989619/1‑149 (MQ=255)
                                                       aGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCa                                                                                             >  2:3681431/1‑149 (MQ=255)
                                                        gCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCAc                                                                                            >  2:697592/1‑149 (MQ=255)
                                                        gCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCAc                                                                                            <  2:2729851/149‑1 (MQ=255)
                                                           aTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACttt                                                                                         >  1:98632/1‑149 (MQ=255)
                                                            tAGACTCCAGCGTCGCGCGTATACTGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCAATTtg                                                                                        <  1:4732040/149‑1 (MQ=255)
                                                            tAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtg                                                                                        <  1:2199628/149‑1 (MQ=255)
                                                            tAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtg                                                                                        >  1:3813662/1‑149 (MQ=255)
                                                            tAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtg                                                                                        <  2:2059692/149‑1 (MQ=255)
                                                            tAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtg                                                                                        >  2:2036148/1‑149 (MQ=255)
                                                            tAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtg                                                                                        >  1:1423721/1‑149 (MQ=255)
                                                             aGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtgg                                                                                       <  2:1733348/149‑1 (MQ=255)
                                                              gACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtggt                                                                                      <  1:2423907/149‑1 (MQ=255)
                                                              gACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtggt                                                                                      <  2:4728640/149‑1 (MQ=255)
                                                              gACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtggt                                                                                      >  1:442159/1‑149 (MQ=255)
                                                              gACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtggt                                                                                      <  2:4729204/149‑1 (MQ=255)
                                                               aCTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtggtg                                                                                     >  1:4045966/1‑149 (MQ=255)
                                                               aCTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtggtg                                                                                     <  1:262351/149‑1 (MQ=255)
                                                               aCTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTtggtg                                                                                     <  1:262264/149‑1 (MQ=255)
                                                                 tCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGc                                                                                   >  2:2212532/1‑149 (MQ=255)
                                                                  ccAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCg                                                                                  <  1:2004089/149‑1 (MQ=255)
                                                                     gCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGaaa                                                                               >  1:3004548/1‑149 (MQ=255)
                                                                      cGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAat                                                                              <  1:508512/149‑1 (MQ=255)
                                                                       gTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAGTGGTGTTTTGCACTTTGGTGGCGAActa                                                                             >  2:4485072/1‑149 (MQ=255)
                                                                         cgcgcgTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATAtt                                                                           >  1:618363/1‑149 (MQ=255)
                                                                         cgcgcgTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATAtt                                                                           <  1:3221325/149‑1 (MQ=255)
                                                                          gcgcgTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTc                                                                          <  1:2237726/149‑1 (MQ=255)
                                                                           cgcgTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCg                                                                         <  2:41655/149‑1 (MQ=255)
                                                                           cgcgTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCg                                                                         <  1:3483633/149‑1 (MQ=255)
                                                                              gTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGaa                                                                      <  1:2670837/149‑1 (MQ=255)
                                                                              gTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGaa                                                                      <  1:1080396/149‑1 (MQ=255)
                                                                               tataATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAAt                                                                     >  1:1283193/1‑149 (MQ=255)
                                                                               tataATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAAt                                                                     <  1:2025012/149‑1 (MQ=255)
                                                                               tataATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAAt                                                                     <  1:2789660/149‑1 (MQ=255)
                                                                               tataATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAAt                                                                     >  2:1419487/1‑149 (MQ=255)
                                                                                 taATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCg                                                                   >  1:4662529/1‑149 (MQ=255)
                                                                                   aTGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTa                                                                 >  1:647064/1‑149 (MQ=255)
                                                                                         ttAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGACTCGTAGGcgtc                                                           >  2:3338960/1‑149 (MQ=255)
                                                                                            gCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGtt                                                        >  2:1130898/1‑149 (MQ=255)
                                                                                            gCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGtt                                                        <  2:846714/149‑1 (MQ=255)
                                                                                              gTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTaa                                                      <  1:1882855/149‑1 (MQ=255)
                                                                                              gTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTaa                                                      <  1:3873697/149‑1 (MQ=255)
                                                                                                  aCCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCaa                                                  <  1:3551324/149‑1 (MQ=255)
                                                                                                  aCCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCaa                                                  <  1:1163127/149‑1 (MQ=255)
                                                                                                   ccccACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAAc                                                 >  1:2462196/1‑149 (MQ=255)
                                                                                                    cccACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACt                                                <  1:1490822/149‑1 (MQ=255)
                                                                                                     ccACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACtt                                               <  2:1982170/149‑1 (MQ=255)
                                                                                                       aCGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACttca                                             >  1:2164570/1‑149 (MQ=255)
                                                                                                       aCGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACttca                                             >  2:617538/1‑149 (MQ=255)
                                                                                                       aCGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACttca                                             >  1:874953/1‑149 (MQ=255)
                                                                                                         gAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACttcatt                                           >  1:1849267/1‑149 (MQ=255)
                                                                                                          aGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACttcattt                                          <  2:320128/149‑1 (MQ=255)
                                                                                                          aGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACttcattt                                          <  2:4193243/149‑1 (MQ=255)
                                                                                                           gTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGGTAATCAACttcatttc                                         >  2:753181/1‑149 (MQ=255)
                                                                                                               gACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGAACGGCGAACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATAGTAGGCGTCGTTAATCAACTTCATTTCATcg                                     >  2:3939512/1‑149 (MQ=255)
                                                                                                                   gAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCa                                 <  2:4047308/149‑1 (MQ=255)
                                                                                                                      aaTCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGcc                              <  2:3271899/149‑1 (MQ=255)
                                                                                                                       aTCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCa                             <  1:2837199/149‑1 (MQ=255)
                                                                                                                        tCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCAc                            <  1:4574238/149‑1 (MQ=255)
                                                                                                                             ccGCGCGCATACGGGTCCCAGGACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAAt                       <  2:2674848/149‑1 (MQ=255)
                                                                                                                               gcgcgcATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATgg                     <  1:1897155/149‑1 (MQ=255)
                                                                                                                                cgcgcATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGa                    <  1:4302510/149‑1 (MQ=255)
                                                                                                                                   gcATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATgc                 <  1:1314096/149‑1 (MQ=255)
                                                                                                                                     aTACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATgcgc               <  1:3402741/149‑1 (MQ=255)
                                                                                                                                      tACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGcc              >  2:965582/1‑149 (MQ=255)
                                                                                                                                      tACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGcc              >  2:1861462/1‑149 (MQ=255)
                                                                                                                                          ggTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGcc          >  1:856205/1‑149 (MQ=255)
                                                                                                                                            tCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCAt        >  2:1328655/1‑149 (MQ=255)
                                                                                                                                              ccAGTTCGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATaa      >  2:3765470/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTGGTGTTTTGCACTTTGGTGGCGAAATATTCGGAATCGTAGGCGTCGTTAATCAACTTCATTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACAC  >  CP009273/4105938‑4106232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑