Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,235,869 G→T 100% A160E (GCG→GAG)  malE ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,235,8690GT99.3% 978.1 / ‑2.0 298A160E (GCG→GAG) malEmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (138/156);  total (140/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 298 total aligned reads displayed.

CGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTG  >  CP009273/4235723‑4236014
                                                                                                                                                  |                                                                                                                                                 
   ccACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATACAGCTCCggg                                                                                                                                              >  1:1719568/1‑149 (MQ=255)
      cGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGAtc                                                                                                                                           <  1:3033609/149‑1 (MQ=255)
       gTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGAtct                                                                                                                                          >  1:2713775/1‑149 (MQ=255)
             aaTGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTccc                                                                                                                                    <  2:1089888/149‑1 (MQ=255)
             aaTGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTccc                                                                                                                                    <  2:4357545/149‑1 (MQ=255)
              aTGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCGTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCa                                                                                                                                   <  1:1028904/149‑1 (MQ=255)
                gTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAgg                                                                                                                                 <  1:453955/149‑1 (MQ=255)
                 tCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGt                                                                                                                                >  2:4511954/1‑149 (MQ=255)
                 tCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGt                                                                                                                                >  1:516415/1‑149 (MQ=255)
                    tACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGtttt                                                                                                                             <  2:4465004/149‑1 (MQ=255)
                    tACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGtttt                                                                                                                             <  1:1002644/149‑1 (MQ=255)
                       ttGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTgg                                                                                                                          <  1:3820650/149‑1 (MQ=255)
                       ttGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTgg                                                                                                                          >  2:4061776/1‑149 (MQ=255)
                             ttttCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTACGGGATCTCTTCCCAGGTTTTTGGCGGGtt                                                                                                                    >  2:3202595/1‑149 (MQ=255)
                              tttCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTc                                                                                                                   <  2:1822305/149‑1 (MQ=255)
                               ttCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCGTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCg                                                                                                                  <  1:4668650/149‑1 (MQ=255)
                                    aCTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGgcagc                                                                                                             >  2:24700/1‑149 (MQ=255)
                                     cTTGAACGCATAACCCCCGTCAGCTGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGgcagca                                                                                                            <  1:3321786/149‑1 (MQ=255)
                                     cTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGgcagca                                                                                                            <  1:1215609/149‑1 (MQ=255)
                                      ttGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGcagcag                                                                                                           >  2:1368596/1‑149 (MQ=255)
                                       tGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGa                                                                                                          <  2:3521861/149‑1 (MQ=255)
                                       tGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGa                                                                                                          <  2:1845974/149‑1 (MQ=255)
                                         aaCGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATc                                                                                                        <  2:2960989/149‑1 (MQ=255)
                                         aaCGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATc                                                                                                        <  2:568762/149‑1 (MQ=255)
                                          aCGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCt                                                                                                       <  1:1671053/149‑1 (MQ=255)
                                            gCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCttt                                                                                                     <  1:4041914/149‑1 (MQ=255)
                                              aTAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCttttt                                                                                                   >  1:2330302/1‑147 (MQ=255)
                                               tAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCtttttt                                                                                                  >  2:3567177/1‑149 (MQ=255)
                                               tAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGGTTTTGGCGGGTTCGGCAGCAGATCtttttt                                                                                                  >  2:3122150/1‑149 (MQ=255)
                                                aaCCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTta                                                                                                 >  1:3371016/1‑149 (MQ=255)
                                                aaCCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTta                                                                                                 <  1:1589333/149‑1 (MQ=255)
                                                  cccccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTtata                                                                                               <  1:1335976/149‑1 (MQ=255)
                                                   ccccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATaa                                                                                              <  1:1248040/149‑1 (MQ=255)
                                                    cccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATaaa                                                                                             <  2:4133653/149‑1 (MQ=255)
                                                    cccGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATaaa                                                                                             <  1:4111130/149‑1 (MQ=255)
                                                      cGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATc                                                                                           <  1:2526233/149‑1 (MQ=255)
                                                           gcagcaATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGa                                                                                      <  1:212464/149‑1 (MQ=255)
                                                           gcagcaATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGa                                                                                      <  1:2019586/149‑1 (MQ=255)
                                                            cagcaATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGAt                                                                                     <  2:4079502/149‑1 (MQ=255)
                                                              gcaATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATaa                                                                                   >  2:4639917/1‑149 (MQ=255)
                                                                 aTCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGc                                                                                <  2:2657938/149‑1 (MQ=255)
                                                                 aTCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGc                                                                                <  2:1741528/149‑1 (MQ=255)
                                                                 aTCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGc                                                                                <  2:93289/149‑1 (MQ=255)
                                                                  tCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCt                                                                               <  1:2992797/149‑1 (MQ=255)
                                                                    aGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTc                                                                             >  1:3338153/1‑149 (MQ=255)
                                                                     gCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCa                                                                            <  1:1913922/149‑1 (MQ=255)
                                                                     gCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCa                                                                            >  2:2309603/1‑149 (MQ=255)
                                                                           aGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCg                                                                      <  1:668576/149‑1 (MQ=255)
                                                                           aGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCg                                                                      >  1:534128/1‑149 (MQ=255)
                                                                            ggTGAAGTACGGTTCTTGCAGGTTGAACATCAGCTCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCATGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGa                                                                     <  1:4265428/149‑1 (MQ=255)
                                                                            ggTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGa                                                                     >  1:3189145/1‑149 (MQ=255)
                                                                            ggTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGa                                                                     >  1:3189097/1‑149 (MQ=255)
                                                                            ggTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCAGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGa                                                                     >  1:797319/1‑149 (MQ=255)
                                                                            ggTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTGATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATACATCAGCGATAACGCTTCAACAGCGa                                                                     <  2:3732412/149‑1 (MQ=255)
                                                                             gTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGAt                                                                    <  2:2038711/149‑1 (MQ=255)
                                                                                aaGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCgg                                                                 >  2:1471461/1‑149 (MQ=255)
                                                                                   tACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTa                                                              >  1:63087/1‑149 (MQ=255)
                                                                                   tACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTa                                                              <  1:1648521/149‑1 (MQ=255)
                                                                                     cGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACCGCGATCGGGTAAg                                                            >  1:1386783/1‑149 (MQ=255)
                                                                                          cTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATc                                                       <  1:1519635/149‑1 (MQ=255)
                                                                                          cTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATc                                                       <  1:1169649/149‑1 (MQ=255)
                                                                                                ggTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTg                                                 >  1:3108199/1‑149 (MQ=255)
                                                                                                 gTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGc                                                <  1:2739745/149‑1 (MQ=255)
                                                                                                 gTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGc                                                >  2:2631134/1‑149 (MQ=255)
                                                                                                  ttGAACATCAGCGCGCTCTGACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGcc                                               <  1:17591/149‑1 (MQ=255)
                                                                                                     aaCATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGtt                                            <  1:2159694/149‑1 (MQ=255)
                                                                                                     aaCATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCATTATAAAGCTCCGGGATCTCATACCAGGTTTTTGGCGGGTTCGGCAGCAGATAATTTTTATAAATCATCGATAACGCTAAAACAGCCAACGGGTAACCAAACACCTAGCAGtt                                            >  1:2918315/1‑149 (MQ=255)
                                                                                                          cAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAAc                                       <  2:1203552/149‑1 (MQ=255)
                                                                                                             cgcgcTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTa                                    <  1:2356297/149‑1 (MQ=255)
                                                                                                              gcgcTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCATATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAATCAAACAGATTGCCGTTGTAACGTAc                                   >  2:2112797/1‑149 (MQ=255)
                                                                                                              gcgcTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTAc                                   >  2:4699637/1‑149 (MQ=255)
                                                                                                                gcTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACgg                                 >  2:118486/1‑149 (MQ=255)
                                                                                                                gcTCTTACCTTTCGCTTTCAGTTCATTATCCAGCTCCGGGAACTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACgg                                 >  2:1665580/1‑149 (MQ=255)
                                                                                                                 ctctTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGc                                >  2:2750864/1‑149 (MQ=255)
                                                                                                                 ctctTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGc                                <  1:4493740/149‑1 (MQ=255)
                                                                                                                   ctTACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCAt                              <  2:1721230/149‑1 (MQ=255)
                                                                                                                    ttACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATc                             <  1:1816874/149‑1 (MQ=255)
                                                                                                                     tACCTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCATCGATAACGCTTCAACATCGATCGGGTAAGCAATAAGCTTGCCGTTGTTACGTACTGCATcc                            >  2:4021623/1‑149 (MQ=255)
                                                                                                                       ccTTTCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCa                          >  2:103154/1‑149 (MQ=255)
                                                                                                                         tttCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAgg                        >  2:3685198/1‑149 (MQ=255)
                                                                                                                          ttCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGt                       <  2:1617305/149‑1 (MQ=255)
                                                                                                                           tCGCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTa                      <  1:4363720/149‑1 (MQ=255)
                                                                                                                             gCTTTCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTaaa                    >  2:891555/1‑149 (MQ=255)
                                                                                                                                 tCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGa                >  2:1547231/1‑149 (MQ=255)
                                                                                                                                 tCAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGa                <  1:4416454/149‑1 (MQ=255)
                                                                                                                                  cAGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGAt               >  1:4767514/1‑149 (MQ=255)
                                                                                                                                   aGTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATa              >  2:293877/1‑149 (MQ=255)
                                                                                                                                    gTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATAc             >  1:1956345/1‑149 (MQ=255)
                                                                                                                                    gTTCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATAc             >  2:1926807/1‑149 (MQ=255)
                                                                                                                                     ttCTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACa            <  1:1196106/149‑1 (MQ=255)
                                                                                                                                       cTTTATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACTGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGc          <  1:3001777/149‑1 (MQ=255)
                                                                                                                                        tttATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCt         <  1:3592289/149‑1 (MQ=255)
                                                                                                                                         ttATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGGTGTAACGTACGGCATCCCAGGTAAACGGATACAGCtt        >  2:3316480/1‑149 (MQ=255)
                                                                                                                                          tATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTg       >  2:3254793/1‑149 (MQ=255)
                                                                                                                                          tATCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTg       <  1:2665406/149‑1 (MQ=255)
                                                                                                                                            tCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTc     >  2:3437871/1‑149 (MQ=255)
                                                                                                                                            tCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTc     >  1:390424/1‑149 (MQ=255)
                                                                                                                                            tCCAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTc     <  2:3280860/149‑1 (MQ=255)
                                                                                                                                             ccAGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTTTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTcc    <  1:896489/149‑1 (MQ=255)
                                                                                                                                               aGCTCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTg  >  1:4567022/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTG  >  CP009273/4235723‑4236014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑