Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,245,537 G→T 87.5% N286K (AAC→AAA)  plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,245,5370GT87.5% 736.9 / 111.2 302N286K (AAC→AAA) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (19/19);  new base T (81/183);  total (100/202)
Fisher's exact test for biased strand distribution p-value = 2.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 304 total aligned reads displayed.

AGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT  >  CP009273/4245391‑4245684
                                                                                                                                                  |                                                                                                                                                   
aGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGAGACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCttt                                                                                                                                                   <  2:1420887/149‑1 (MQ=255)
   ttGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATg                                                                                                                                                >  1:3865951/1‑149 (MQ=255)
   ttGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATg                                                                                                                                                >  2:2706248/1‑149 (MQ=255)
    tGATCCCGGCGGCGATATGCGGCGGCACCAGCCGCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATgg                                                                                                                                               <  2:3858962/149‑1 (MQ=255)
     gATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGa                                                                                                                                              >  2:2185465/1‑149 (MQ=255)
      aTCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGAc                                                                                                                                             <  1:3996371/149‑1 (MQ=255)
           ggcggcGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGa                                                                                                                                        <  1:1588761/149‑1 (MQ=255)
            gcggcgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGAt                                                                                                                                       <  2:2863870/149‑1 (MQ=255)
                cgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGccc                                                                                                                                   <  1:3785314/149‑1 (MQ=255)
                cgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGccc                                                                                                                                   <  1:2131441/149‑1 (MQ=255)
                 gATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCt                                                                                                                                  <  2:3609214/149‑1 (MQ=255)
                  atatGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTg                                                                                                                                 >  1:3259082/1‑149 (MQ=255)
                   tatGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTgg                                                                                                                                >  2:1294250/1‑149 (MQ=255)
                   tatGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTgg                                                                                                                                <  1:4635937/149‑1 (MQ=255)
                         gcggcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGt                                                                                                                          <  2:375443/149‑1 (MQ=255)
                          cggcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTc                                                                                                                         <  1:1675604/149‑1 (MQ=255)
                           ggcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCg                                                                                                                        <  2:690991/149‑1 (MQ=255)
                           ggcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCg                                                                                                                        >  2:4225333/1‑149 (MQ=255)
                            gcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCgg                                                                                                                       <  2:1012045/149‑1 (MQ=255)
                            gcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCgg                                                                                                                       >  1:3620060/1‑149 (MQ=255)
                            gcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCgg                                                                                                                       <  2:3367181/149‑1 (MQ=255)
                                cAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc                                                                                                                   <  1:2767742/149‑1 (MQ=255)
                                cAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc                                                                                                                   <  2:2836629/149‑1 (MQ=255)
                                 aGCCCTTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCa                                                                                                                  <  2:1186061/149‑1 (MQ=255)
                                 aGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCa                                                                                                                  <  1:1137766/149‑1 (MQ=255)
                                 aGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCa                                                                                                                  <  1:1003805/149‑1 (MQ=255)
                                  gCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAg                                                                                                                 <  2:3696526/149‑1 (MQ=255)
                                  gCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAg                                                                                                                 <  2:4467318/149‑1 (MQ=255)
                                  gCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAg                                                                                                                 <  2:2069687/149‑1 (MQ=255)
                                     ccTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTg                                                                                                              <  1:695333/149‑1 (MQ=255)
                                     ccTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTg                                                                                                              <  2:4070021/149‑1 (MQ=255)
                                      cTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGa                                                                                                             >  2:760800/1‑149 (MQ=255)
                                       tGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGaa                                                                                                            <  2:2764669/149‑1 (MQ=255)
                                        ggTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAg                                                                                                           >  2:1857244/1‑149 (MQ=255)
                                            aTACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCa                                                                                                       >  2:555217/1‑149 (MQ=255)
                                                aGCACGTAAGAAAGCTGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAAt                                                                                                   <  2:419754/149‑1 (MQ=255)
                                                 gCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATa                                                                                                  <  1:479446/149‑1 (MQ=255)
                                                    cGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACgg                                                                                               >  2:3579325/1‑149 (MQ=255)
                                                     gTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGTCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgt                                                                                              <  1:4265293/149‑1 (MQ=255)
                                                       aaGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtca                                                                                            >  1:1647038/1‑149 (MQ=255)
                                                        aGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcag                                                                                           <  1:4560706/149‑1 (MQ=255)
                                                          aaaGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagtc                                                                                         >  1:2552160/1‑149 (MQ=255)
                                                           aaGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagtca                                                                                        >  2:3181518/1‑149 (MQ=255)
                                                              cagcagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGc                                                                                     <  2:3405192/149‑1 (MQ=255)
                                                              cagcagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGc                                                                                     >  2:4391956/1‑149 (MQ=255)
                                                                gcagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGa                                                                                   >  1:3116381/1‑149 (MQ=255)
                                                                gcagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGa                                                                                   <  2:329814/149‑1 (MQ=255)
                                                                 cagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGaa                                                                                  <  1:2485236/149‑1 (MQ=255)
                                                                 cagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGaa                                                                                  >  2:2666401/1‑149 (MQ=255)
                                                                    gTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatca                                                                               <  1:4120859/149‑1 (MQ=255)
                                                                      aGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcatc                                                                             >  2:1841229/1‑149 (MQ=255)
                                                                       gTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAtct                                                                            >  2:112157/1‑149 (MQ=255)
                                                                       gTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAtct                                                                            <  1:314227/149‑1 (MQ=255)
                                                                           aTGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTa                                                                        >  1:4166508/1‑149 (MQ=255)
                                                                           aTGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTa                                                                        <  2:2436833/149‑1 (MQ=255)
                                                                            tgtgACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTaa                                                                       <  2:3262120/149‑1 (MQ=255)
                                                                                aCTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAgaga                                                                   <  2:2623837/149‑1 (MQ=255)
                                                                                aCTGCGGTGGAAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGATCGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAgaga                                                                   <  2:4383410/149‑1 (MQ=255)
                                                                                 cTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGaa                                                                  <  2:3331276/149‑1 (MQ=255)
                                                                                   gCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAAt                                                                <  1:2061053/149‑1 (MQ=255)
                                                                                    cGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAAtt                                                               <  1:4338406/149‑1 (MQ=255)
                                                                                          cAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgccgc                                                         <  2:458815/149‑1 (MQ=255)
                                                                                          cAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgccgc                                                         <  2:58579/149‑1 (MQ=255)
                                                                                           aaGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCa                                                        >  1:2944425/1‑149 (MQ=255)
                                                                                                acaTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAtctc                                                   <  2:103719/149‑1 (MQ=255)
                                                                                                acaTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCAAGAATACGGTCAGTCAGGCGAATCATATCGTAAGAGAAATTCGCCGCAAtctc                                                   <  1:1080314/149‑1 (MQ=255)
                                                                                                 caTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATctct                                                  >  2:1782843/1‑149 (MQ=255)
                                                                                                   tataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTc                                                >  1:1443336/1‑149 (MQ=255)
                                                                                                   tataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCACAATCTCTTc                                                >  1:1070171/1‑149 (MQ=255)
                                                                                                      aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAt                                             <  1:2824440/149‑1 (MQ=255)
                                                                                                         aGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAg                                          >  1:1164817/1‑149 (MQ=255)
                                                                                                         aGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAg                                          <  1:291286/149‑1 (MQ=255)
                                                                                                         aGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAg                                          <  1:2325540/149‑1 (MQ=255)
                                                                                                            tCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGc                                       <  1:3527640/149‑1 (MQ=255)
                                                                                                            tCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGc                                       <  1:4352945/149‑1 (MQ=255)
                                                                                                            tCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGc                                       >  2:3439254/1‑149 (MQ=255)
                                                                                                               tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCact                                    >  1:1381094/1‑147 (MQ=255)
                                                                                                               tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAAt                                    <  2:1635256/149‑1 (MQ=255)
                                                                                                                  ccGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgc                                 <  1:3399815/149‑1 (MQ=255)
                                                                                                                  ccGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgc                                 <  1:2017852/149‑1 (MQ=255)
                                                                                                                    gtcgtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGt                               >  2:935427/1‑149 (MQ=255)
                                                                                                                    gtcgtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGt                               >  1:501418/1‑149 (MQ=255)
                                                                                                                      cgtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc                             <  1:868865/149‑1 (MQ=255)
                                                                                                                       gtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTct                            <  1:4714008/149‑1 (MQ=255)
                                                                                                                       gtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTct                            <  2:1148425/149‑1 (MQ=255)
                                                                                                                          ggCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctgct                         <  1:4487333/149‑1 (MQ=255)
                                                                                                                               gCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCt                    <  2:3508209/149‑1 (MQ=255)
                                                                                                                                cTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCtt                   <  1:4655521/149‑1 (MQ=255)
                                                                                                                                 tGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttt                  >  2:4647299/1‑149 (MQ=255)
                                                                                                                                   gCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt                <  1:3467701/149‑1 (MQ=255)
                                                                                                                                   gCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt                <  1:2289676/149‑1 (MQ=255)
                                                                                                                                     gAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCa              >  1:1846588/1‑149 (MQ=255)
                                                                                                                                      aaCGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCAt             >  1:2397760/1‑149 (MQ=255)
                                                                                                                                      aaCGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCAt             <  2:2219873/149‑1 (MQ=255)
                                                                                                                                       aCGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATg            <  2:428593/149‑1 (MQ=255)
                                                                                                                                       aCGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTATTTCATg            >  2:3042531/1‑149 (MQ=255)
                                                                                                                                        cgcgCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATgg           <  2:3776797/149‑1 (MQ=255)
                                                                                                                                          cgcTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGga         <  2:1820210/149‑1 (MQ=255)
                                                                                                                                              cAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAtt     >  2:2362271/1‑149 (MQ=255)
                                                                                                                                              cAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAtt     >  1:3234579/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT  >  CP009273/4245391‑4245684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑