Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,275,862 C→A 82.4% D480Y (GAT→TAT)  acs ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,275,8620CA82.4% 581.0 / 154.2 283D480Y (GAT→TAT) acsacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (36/14);  new base A (128/105);  total (164/119)
Fisher's exact test for biased strand distribution p-value = 2.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 283 total aligned reads displayed.

CCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGC  >  CP009273/4275717‑4276007
                                                                                                                                                 |                                                                                                                                                 
cccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGcc                                                                                                                                                >  2:1499245/1‑149 (MQ=255)
   aGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCaaa                                                                                                                                             <  1:3837470/149‑1 (MQ=255)
       ggTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGc                                                                                                                                         <  1:2205264/149‑1 (MQ=255)
         tGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGt                                                                                                                                       <  1:795147/149‑1 (MQ=255)
          gACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTa                                                                                                                                      >  1:3254555/1‑149 (MQ=255)
           aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTAc                                                                                                                                     <  2:786564/149‑1 (MQ=255)
           aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTAc                                                                                                                                     <  2:3771820/149‑1 (MQ=255)
             cGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAcgc                                                                                                                                   >  1:2682659/1‑149 (MQ=255)
             cGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTAcgc                                                                                                                                   <  2:2946674/149‑1 (MQ=255)
                agaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGcc                                                                                                                                <  1:2890599/149‑1 (MQ=255)
                 gaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGACATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCt                                                                                                                               >  1:1402544/1‑149 (MQ=255)
                 gaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCt                                                                                                                               >  1:3792111/1‑149 (MQ=255)
                 gaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCt                                                                                                                               >  2:3794697/1‑149 (MQ=255)
                  aCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTg                                                                                                                              >  2:4196568/1‑149 (MQ=255)
                    gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGAc                                                                                                                            <  1:3380049/149‑1 (MQ=255)
                     ttCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGAcc                                                                                                                           >  1:2329047/1‑149 (MQ=255)
                             gtcgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAgg                                                                                                                   <  2:399811/149‑1 (MQ=255)
                              tcgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGa                                                                                                                  >  1:312814/1‑149 (MQ=255)
                              tcgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGa                                                                                                                  >  2:2714484/1‑149 (MQ=255)
                                 tcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTc                                                                                                               <  1:4057420/149‑1 (MQ=255)
                                   cacaCGCCCGGTTATCCAGTAATAGCCATCTTCATCTCGAAGCGCGCCGTCGCCGCTGAAATACATATTTTTGAATGTGGACAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACATCGAACGCGCCTGACCCGCCCAGGAGTCgg                                                                                                             >  1:2034509/1‑149 (MQ=255)
                                   cacaCGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCgg                                                                                                             >  2:2208941/1‑149 (MQ=255)
                                    acacGCCCGGTTATCCAGTAAGAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGt                                                                                                            <  2:123248/149‑1 (MQ=255)
                                     cacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTg                                                                                                           <  2:1808093/149‑1 (MQ=255)
                                     cacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTg                                                                                                           >  2:2787911/1‑149 (MQ=255)
                                        gCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                                                                                                        >  2:3166310/1‑149 (MQ=255)
                                        gCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                                                                                                        <  2:3035182/149‑1 (MQ=255)
                                        gCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                                                                                                        >  1:1287618/1‑149 (MQ=255)
                                         cccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTa                                                                                                       <  1:4677046/149‑1 (MQ=255)
                                            ggTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCa                                                                                                    <  1:436938/149‑1 (MQ=255)
                                            ggTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCa                                                                                                    <  1:2502901/149‑1 (MQ=255)
                                                aTCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCt                                                                                                <  2:483377/149‑1 (MQ=255)
                                                   cAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTAcc                                                                                             <  1:2957755/149‑1 (MQ=255)
                                                   cAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTAcc                                                                                             <  1:262627/149‑1 (MQ=255)
                                                   cAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTAcc                                                                                             >  1:3216274/1‑149 (MQ=255)
                                                      tAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTc                                                                                          <  1:1662519/149‑1 (MQ=255)
                                                      tAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTc                                                                                          >  1:2458788/1‑149 (MQ=255)
                                                           gCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTgg                                                                                     >  2:1402057/1‑149 (MQ=255)
                                                               tCTTCATCGCGACGCGCGCCGTCGCCGCTGATATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcccc                                                                                 <  1:2867201/149‑1 (MQ=255)
                                                               tCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcccc                                                                                 >  2:3892395/1‑149 (MQ=255)
                                                                 ttCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCt                                                                               <  1:4038477/149‑1 (MQ=255)
                                                                   cATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAGGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTcc                                                                             >  1:1200341/1‑149 (MQ=255)
                                                                    aTCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCa                                                                            <  1:1053277/149‑1 (MQ=255)
                                                                    aTCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCa                                                                            <  2:2256504/149‑1 (MQ=255)
                                                                     tCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAg                                                                           >  2:356843/1‑149 (MQ=255)
                                                                     tCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAg                                                                           >  2:4745582/1‑149 (MQ=255)
                                                                         gACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCggg                                                                       >  1:2637628/1‑149 (MQ=255)
                                                                         gACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCggg                                                                       >  1:1058461/1‑149 (MQ=255)
                                                                          aCGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGt                                                                      <  1:4065952/149‑1 (MQ=255)
                                                                             cgcgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTAc                                                                   <  2:1584465/149‑1 (MQ=255)
                                                                                 ccGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTc                                                               <  1:2097436/149‑1 (MQ=255)
                                                                                  cGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGGGGCCCCCTCCAGCGGGTTACCTTCg                                                              <  2:1184092/149‑1 (MQ=255)
                                                                                   gTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGt                                                             <  1:4550525/149‑1 (MQ=255)
                                                                                   gTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGt                                                             <  1:3095163/149‑1 (MQ=255)
                                                                                    tCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGtt                                                            <  1:4521779/149‑1 (MQ=255)
                                                                                      gccgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTAt                                                          <  1:50488/149‑1 (MQ=255)
                                                                                         gcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGa                                                       <  1:3443468/149‑1 (MQ=255)
                                                                                          cTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGAc                                                      <  1:3794627/149‑1 (MQ=255)
                                                                                           tGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGAcc                                                     >  1:464541/1‑149 (MQ=255)
                                                                                           tGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGAcc                                                     >  1:3042266/1‑149 (MQ=255)
                                                                                            gAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCa                                                    >  1:4712949/1‑149 (MQ=255)
                                                                                              aaTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAgc                                                  <  1:1255260/149‑1 (MQ=255)
                                                                                              aaTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAgc                                                  <  2:1883684/149‑1 (MQ=255)
                                                                                               aTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAgcg                                                 >  2:202679/1‑149 (MQ=255)
                                                                                                 aCATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGcc                                               <  1:3488157/149‑1 (MQ=255)
                                                                                                      tttttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTg                                          >  1:3583960/1‑149 (MQ=255)
                                                                                                      tttttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTg                                          >  2:4572178/1‑149 (MQ=255)
                                                                                                        tttGAAGGTGGAGAAGTAGGTCTGTTCAACACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCa                                        <  2:1146411/149‑1 (MQ=255)
                                                                                                        tttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCa                                        >  2:37143/1‑149 (MQ=255)
                                                                                                         ttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCAc                                       <  1:1481750/149‑1 (MQ=255)
                                                                                                             aGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCg                                   >  2:1676243/1‑149 (MQ=255)
                                                                                                             aGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCg                                   >  1:1702465/1‑149 (MQ=255)
                                                                                                             aGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCg                                   >  2:209879/1‑149 (MQ=255)
                                                                                                             aGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCg                                   <  2:286806/149‑1 (MQ=255)
                                                                                                                tGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaag                                >  2:1859539/1‑149 (MQ=255)
                                                                                                                tGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaag                                <  2:894906/149‑1 (MQ=255)
                                                                                                                 ggAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaaga                               >  1:3966124/1‑149 (MQ=255)
                                                                                                                    gaAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACg                            >  2:3872233/1‑149 (MQ=255)
                                                                                                                      aGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGa                          <  1:3762882/149‑1 (MQ=255)
                                                                                                                       gTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGAc                         >  2:1660530/1‑149 (MQ=255)
                                                                                                                        tAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGGTGCACGCCGAAGAACGGACg                        >  1:3595766/1‑149 (MQ=255)
                                                                                                                        tAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACg                        >  1:9865/1‑149 (MQ=255)
                                                                                                                         aGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgt                       >  2:2181612/1‑149 (MQ=255)
                                                                                                                          ggTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgtg                      >  1:619916/1‑149 (MQ=255)
                                                                                                                           gTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgtgt                     >  1:4086322/1‑149 (MQ=255)
                                                                                                                           gTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgtgt                     <  2:3451076/149‑1 (MQ=255)
                                                                                                                              tGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGc                  <  1:2838759/149‑1 (MQ=255)
                                                                                                                              tGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGc                  <  1:4390130/149‑1 (MQ=255)
                                                                                                                               gTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGcc                 >  1:4740749/1‑149 (MQ=255)
                                                                                                                               gTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGcc                 >  1:2389297/1‑149 (MQ=255)
                                                                                                                                    aaaCGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAAcc            >  1:1555215/1‑149 (MQ=255)
                                                                                                                                      aCGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCgg          >  2:2527979/1‑149 (MQ=255)
                                                                                                                                      aCGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCgg          >  2:2301330/1‑149 (MQ=255)
                                                                                                                                       cGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCATCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGc         >  1:1636665/1‑149 (MQ=255)
                                                                                                                                       cGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGc         <  1:945325/149‑1 (MQ=255)
                                                                                                                                        gTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCt        >  1:719847/1‑149 (MQ=255)
                                                                                                                                        gTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCt        >  1:2780459/1‑149 (MQ=255)
                                                                                                                                          tCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCttt      <  1:1975726/149‑1 (MQ=255)
                                                                                                                                            gTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCa    >  1:4173109/1‑149 (MQ=255)
                                                                                                                                              gATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGc  <  1:762935/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGC  >  CP009273/4275717‑4276007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑